<pre>LOCUS       KJ755553               11714 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain N 87 serotype O132:K+:H28 O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ755553
VERSION     KJ755553.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11714)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11714)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Liu,Y. and Debroy,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: Average 50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="N 87"
                     /serotype="O132:K+:H28"
                     /db_xref="taxon:562"
     misc_feature    1..11714
                     /note="O-antigen gene cluster"
     gene            300..1193
                     /gene="rmlB"
     CDS             300..1193
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AIG62495.1"
                     /translation="MARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIVGTYVLL
                     EAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTAYAPSSP
                     YSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKGLPIY
                     GKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVP
                     KEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWYLSNTKW
                     VDNVKSGAYQSWIEQNYEGRQ"
     gene            1193..2092
                     /gene="rmlD"
     CDS             1193..2092
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="AIG62494.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALKKPEVAGLYHLVAS
                     GTTTWYDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            2150..3028
                     /gene="rmlA"
     CDS             2150..3028
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AIG62493.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKRFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            3033..3563
                     /gene="rmlC"
     CDS             3033..3563
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="AIG62492.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSCKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFLYKTTNYYAPIYEKSILWSDNKINIAWPLQ
                     GGFILSDKDRNASCLE"
     gene            3592..4866
                     /gene="wzx"
     CDS             3592..4866
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62491.1"
                     /translation="MNVIKSRVFLNAVYLFVVQGVTYLVPLITLPYLVRVLGAQSYGV
                     LSFSLAIIQYFILLTDYGFNLSATQKISVICGDINKISNVFWSVTCCKILLMIISAIL
                     VYTVTKVFDVMNGHELVIFSCFGLVLGNILFPVWFFQGKEQMGLSSISNIISKLLAIP
                     LIFLLVNNPNDAWIAAIITSFTSISGGLISLTIIYHKKWVHWVGIRDLKIIQEFKDGW
                     HLFISTAAISMYTTSIVVVLGIFSGPISVGYFTAADRIRQAVQGLITPITQACYPRIN
                     YLYRTSPEKGFELVRKLLYFQGGITLTLSIILMMFASVIVKILFGNGFERSDSVLTIM
                     AFCPFIVGISNVLGIQILVALGYKKVFSKILLCSGGLCLILIGPSTYLYSENGAALSV
                     LITEVFVTIAMLIVVLAKKVPIFKRENLNNEV"
     gene            4856..5956
                     /gene="glf"
     CDS             4856..5956
                     /gene="glf"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="AIG62490.1"
                     /translation="MKYDYIIVGAGLYGAVLACELHKAGKKVMVIERREHIGGNIYTE
                     NVEGIHVHKYGAHIFHTNDKIIWDYVNSFVEFNRFTNSPLANYKGALYNLPFNMNTFY
                     QLWGVRTPDEAIKKINEQKQEINAKTPSNLEEQAISLVGRDIYERFIKGYTEKQWGRK
                     ATELPAFIIKRIPVRFTFDNNYFSDRYQGIPIGGYSGLVEKMLDGVDVHLNVDYLENR
                     DYFDSIADRIIYTGPIDAFYNYCFGYLEYRTLLFKTEIIKRPNFQGNAVINYTERDIP
                     FTRIIEHKHFDYIDSQLTVITKEFPGEWKPGDEPYYPVNDDKNMTIYKKYRELSKKNT
                     KFVFGGRLAEYKYYDMHQVIRSALNQVKLEIN"
     gene            5958..6764
                     /gene="wbbL"
     CDS             5958..6764
                     /gene="wbbL"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="AIG62489.1"
                     /translation="MLYCIIVSHNHEQHVKNLLSFADYNPCSDVKILVKDNIGNDELK
                     RVCEKYEVEYFCNYKKMGFGQNNNEMVSKIINKHKINDNLDYVLFLNPDVLIKHEVLV
                     SFYKYIISKNIKASTIDLFKNEDFTKRDEFIRKFPTLTDFVSSIIFNINKTKIDRSKI
                     IEPCEIDWCAGSFLCINLAIFRDIMGFDERYYMYCEDIDLCYRLKLKKIKLVYLPQFK
                     AIHLAMHENRKILSKAFRWHVSNAIYFIIKKSIFKSFDSYSNSVKTIFRG"
     gene            6766..7977
                     /gene="glgA"
     CDS             6766..7977
                     /gene="glgA"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="AIG62488.1"
                     /translation="MKVLHVATGFPFLYVGGITNYVRSLVISQRNAGHEVHVLSGGCS
                     SENLEYVTNIYSKKIKPFSLKLSIEDEKYAKEVLNICREYDIVHFHMVLDFPSSLLSR
                     LSKEIPKYVVSLHDYFLICPRIFMSDYKNNICHEVNIDKCNVCCGYFDSIDIARKISS
                     KLSIPLPRIKTNEPARRIENVKTFLEGASLLLPVSEKVKEIYSKLVPNASYKVLHIGN
                     ITATELPLPKEENGKIIIGILGTLSYIKGSDIILKIIKQTTNKNIEFHFYGRADKKWL
                     TRLEKYGLINKGTYVQENLKEIISTINLGLVVPIWEDNAPQVVMEFLNLKTPVLATSR
                     GGIPDFIQHLVNGYLFDPDDQCSFQAFIAWINNLNRKTIVQLNAGVKKLKTPEQHEKE
                     IDRVYHVIKNI"
     gene            7958..8539
                     /gene="lacA"
     CDS             7958..8539
                     /gene="lacA"
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="AIG62487.1"
                     /translation="MLSKIYKYGLVASIIIFFSKLKNSIINSSYKRRFGIKNISIGPG
                     GFISNSSRIYFSDNFYAGTNLWIENVKNKGIIVIGSNCRFSNNVHIACINRITIGKGV
                     LIGSNVLITDHSHGKLVAEEKFISPYERTLFSKGEVIIGDNVWICDGVMILPGVTIGS
                     GAIIAANSVVVSNIPSFTVAAGTPAKVIKCIGA"
     gene            8524..9723
                     /gene="wzy"
     CDS             8524..9723
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG62486.1"
                     /translation="MHWGVRTTSIILILLTVFFPGIVLWGGNFKIELIVLCIMSLCLI
                     LRINQVLLCIKPISIIIFSYMLYVLLGGTGLFSGTFDFFSLRNILYPFIKALVLVIFL
                     FLFLLKKTQTPLYDINRLILLCFFIDSIFKVIEIMFPSVVSAWLNIISIDDWWKDTLG
                     MRNLGFRGVSIYDYSVAATLAVLIDFSLRSRKKINFIIYPLVLLSALSSGRTGLVVIF
                     IFTLVYFYRYAPKIIISGFIFIILIMYLSLFHSNELIHNQQLSWMFEPLLNILNGNIE
                     TESTDDLLENHLFMPDNILGYGLWGQYGDMLGSTIRGSDSGYILIILYGGWVGFIFFL
                     ICSFSYLLYAYIKSQDKKLSIAIFFTFMLIMIKGPIIYSDYNAFILMSLLIAPSFYIF
                     YKAKENE"
     CDS             9716..10576
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="AIG62485.1"
                     /translation="MSNNYYIVCLVVIYKEKIFASGTIRSLLHSFSELKDTNKKILVK
                     LWDNSPDSQSSTDLNRLKELFLQNDILLSYQHQKNNYPLSIVYNHFFQEFIHQARFLM
                     LLDQDSIFGPEYFQEFYNAEKRSNVDLFLPKIMHKGRIISPTKIYYAKGFYYKEIASG
                     FISSKKLGAINSGMIISSKYLIRTRFSYDIRLGNYCTDDFFMKKFRENKGIAYILDYS
                     FDHDLTLSTLNDNSDSLRRRYEEMIKGKRLVYSNNIFEMIFIRLYIILNRCYISLKYK
                     DLKYLKGFYE"
     CDS             10569..11561
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferases family 1"
                     /protein_id="AIG62484.1"
                     /translation="MNKLIISIPQFNLSGGNLVSLELGLFLKKKGLDVYGHSGFRLKK
                     IDEIELNIPKRGLCNTIINIISFFCSSFYSLFSKYYIATHHLTSLFNFIKPAKFSLIQ
                     DIESLFYPKRLSWFGTILWRNYLKSDTLLYTSKYLEQRIHNNNNPKTIGFPFIKTSTN
                     FSLTGEKIYDALMILRDGDYKNYNLTLSEFYRLNNNGFTVALVNASRNSLPFNPLIFS
                     NLCRDDFIKVLMMSKRFICLSKWEGLGLPNLEAYSLGLEIISTKIPSAQIIEEIDPNA
                     ITIIDSIEQVEKAIISNRVQISDNKMYLRSESLTDLDSLWKEHIFSTIRYYMGQ"
ORIGIN      
        1 ctggtagctg taaagccagg ggcggtagcg tgcattaata cctctattaa tcaaactgag
       61 agccgcttat ttcacagcat gctctgaagt aatatggaat aataaagtga agatacttgt
      121 tactggtggc gcaggattta ttggttctgc tgtagttcgt cacattataa ataatacgca
      181 ggatagtgtt gttaatgtcg ataaattaac gtacgccagg aaacctggaa tcgcttgctg
      241 atgtttctga ttctgaacgc tatgtttttg aacatgcgga tatttgcgat gcagctgcaa
      301 tggcacggat ttttgctcag catcagccgg atgcagtgat gcacctggct gctgaaagcc
      361 atgtggatcg ttcaattaca ggccctgcgg catttattga aaccaatatt gttggtactt
      421 atgtcctttt ggaagcggct cgcaattact ggtctgctct tgatggcgac aagaaaaata
      481 gcttccgttt tcatcatatt tctactgacg aagtctatgg tgatttgcct catcctgacg
      541 aagtaaataa taaagaacaa ttacccctct ttactgagac gacagcttac gcgccaagta
      601 gcccatattc tgcttcgaaa gcatcaagcg atcatttagt ccgcgcttgg aaacgtacct
      661 atggtttacc gaccattgtg actaattgct ctaacaatta tggtccttat catttcccgg
      721 aaaaattgat tccattggtt attctgaatg ctctggaagg taaaggatta cctatttatg
      781 gtaaagggga tcaaattcgc gactggctgt atgttgaaga tcatgcgcgt gcgttatata
      841 ccgtcgtaac cgaaggtaaa gcgggtgaaa cttataacat tggtggacac aacgaaaaga
      901 aaaacatcga tgtagtgctc actatttgtg atttgttgga tgagattgtc ccgaaagaga
      961 aatcttaccg cgagcaaatt acttatgtcg ccgatcgtcc gggacacgat cgacgttatg
     1021 caattgatgc tgagaagatt ggtcgcgaat tgggatggaa accacaggaa acgtttgaga
     1081 gcgggattcg gaagacagtg gaatggtacc tgtccaatac aaaatgggtt gataatgtga
     1141 aaagtggtgc ctatcaatcg tggattgaac agaactatga gggccgccag taatgaatat
     1201 tctccttttc ggcaaaacag ggcaggtagg ttgggaacta cagcgtgctc tggcaccttt
     1261 gggtaatttg attgctcttg atgttcactc cactgattat tgtggtgatt ttagtaatcc
     1321 tgaaggtgtg gctgaaaccg tcaaaaaaat tcgccctgat gttattgtta atgctgcggc
     1381 tcataccgca gtagataagg ctgagtcaga acccgaattt gcacaattac tcaatgcgac
     1441 cagcgttgaa tcaattgcaa aagcggctaa tgaagttggg gcctgggtaa ttcattactc
     1501 aactgactac gtatttccag gaaccggtga aataccatgg ctggagacgg atgcaaccgc
     1561 accgctaaat gtttacggtg aaaccaagtt agccggagaa aaagcgttac aggaacattg
     1621 cgcgaagcat cttattttcc gtaccagctg ggtatacgca ggtaaaggaa ataactttgc
     1681 caaaacgatg ttgcgtctgg caaaagagcg tgaagaatta gcggttatta acgatcagtt
     1741 tggtgcacca acgggtgctg agctgctggc tgattgtacg gcacatgcta ttcgtgttgc
     1801 actgaaaaaa ccagaagtcg caggcttgta ccatttggta gccagtggta ccacaacctg
     1861 gtacgattat gctgcgctgg tttttgaaga ggcgcgcaaa gcaggcattc cccttgcact
     1921 caacaagctc aacgcagtac caacaactgc ctatcctaca ccagctcgtc gtccacataa
     1981 ctctcgcctt aatacagaaa aatttcagca gaactttgcg cttgtcttgc ctgactggca
     2041 ggttggcgtg aaacgaatgc tcaacgaatt atttacgact acagcaattt aatagttttt
     2101 gcatcttgtt cgtgatggtg gagcaagatg aattaaaagg aatgatgaaa tgaaaacgcg
     2161 taaaggtatt attttagcgg gtggttctgg tacacgtctt tatcctgtga ctatggctgt
     2221 cagtaaacag ctgttaccga tttatgataa accgatgatc tattacccgc tctctacact
     2281 gatgttggcg ggtattcgcg atattctgat tatcagtacg ccacaggata ctcctcgttt
     2341 tcaacaactg ctgggtgacg ggagccagtg ggggctaaat cttcagtaca aagtgcaacc
     2401 gagtccagat ggtcttgcgc aggcatttat catcggtgaa gagtttatcg gtggtgatga
     2461 ttgtgctttg gttctaggtg ataatatctt ttacggtcac gatctgccga agttaatgga
     2521 tgtcgctgtt aacaaagaaa gtggtgcaac ggtatttgcc tatcacgtta atgatcctga
     2581 acgctacggt gtcgttgagt ttgataaaaa cggtacggca ataagcctgg aagaaaaacc
     2641 gttacaacca aaaagtaatt atgcggtaac cgggctttat ttctatgata acgacgttgt
     2701 cgaaatggcg aaaaatctta agccttctgc ccgcggtgaa ctggaaatta ccgatattaa
     2761 ccgtatttat atggaacagg ggcgtttatc cgttgccatg atggggcgtg gatatgcatg
     2821 gctggacacg ggaacacatc aaagtcttat tgaagcaagc aactttattg caacaattga
     2881 agaacgtcag ggactgaaag tttcctgtcc ggaagaaatt gcttaccgta aacggtttat
     2941 tgatgctgag caggtgaaag tattagccga accgctgaag aaaaatgctt atggtcagta
     3001 tctgctcaaa atgattaaag gttattaata aaatgaacgt aattaaaact gaaattccag
     3061 acgtactgat ttttgaaccg aaagtttttg gtgatgagcg tggtttcttt tttgagagct
     3121 ttaaccagaa ggtttttgag gaagctgtag gccgcaaagt tgaatttgtc caagataacc
     3181 attcgaagtc ttgtaaaggt gttttacgcg ggctgcatta tcagttagaa ccttatgcac
     3241 aagggaaatt ggtgcgctgc gttgttggtg aagtttttga cgtagctgtt gatattcgta
     3301 aatcgtcacc aacttttggc aaatgggttg gggtgaattt atctgcagag aataagcgcc
     3361 agttatggat ccctgaagga tttgcacatg gatttttggt gctgagtgag acggcagagt
     3421 ttttgtataa gacgacgaat tactatgcac cgatatatga aaaaagtatt ctttggtctg
     3481 ataataaaat taatattgct tggcctcttc aaggtggttt tattctatcc gataaagatc
     3541 gtaatgcatc gtgtttagaa taatgtataa gcgagtcata ccttgattta tatgaatgta
     3601 attaagagta gagtgttcct aaatgctgta tatctatttg ttgtacaggg cgtaacatat
     3661 ttagttccct taattactct accctattta gttcgggtat taggagcgca aagttacgga
     3721 gtattaagtt tttcactggc aatcatacag tattttatac ttttgaccga ttatggtttt
     3781 aacctatctg caacacaaaa aatttcagtt atatgtggtg atataaataa aatcagcaat
     3841 gtattttggt cggtaacatg ttgtaagatt ttgctgatga taatttcagc tatactagtt
     3901 tatactgtta ccaaagtatt tgatgtgatg aatggtcatg aattagtaat attttcatgc
     3961 tttggacttg tattaggaaa tattctgttt ccagtatggt tttttcaagg gaaggagcaa
     4021 atggggttga gctcgatctc gaatataata tcgaagctat tggcaatccc tctgatcttc
     4081 ttattggtca ataatcctaa tgatgcatgg attgctgcaa taataacatc ttttacttcg
     4141 atatctggtg gattaatttc actaactata atttaccata aaaaatgggt acattgggtt
     4201 ggtataagag atctaaaaat tattcaggaa tttaaagatg gatggcattt atttatttca
     4261 actgcggcaa taagcatgta taccacaagt attgttgtag ttttaggaat tttttctgga
     4321 ccaatttccg tcggttattt tactgctgca gatcgtatta ggcaagcagt tcaggggtta
     4381 attaccccta ttacgcaggc atgctatcca aggattaatt atctttacag aacttctcca
     4441 gaaaagggct ttgagctagt cagaaaatta ttatattttc agggaggtat aaccttaacg
     4501 ttaagtataa tactcatgat gttcgcaagt gttatcgtta aaatcttatt tggaaacggt
     4561 ttcgaaagat cagattctgt actaaccatt atggcatttt gcccattcat agtagggata
     4621 agtaatgtgt taggaattca gatattagtt gcattaggat ataaaaaagt attcagtaaa
     4681 atacttttat gctcaggcgg gttatgtttg atattgattg gcccgtctac atatctctat
     4741 agtgagaatg gtgcagcatt aagtgtgcta ataactgagg tttttgtgac gattgcaatg
     4801 cttattgttg tgcttgcaaa aaaagtccct atttttaaaa gagagaattt gaataatgaa
     4861 gtatgattac attattgttg gagctggtct ctacggcgct gtcctggcct gtgaacttca
     4921 taaggctgga aaaaaagtta tggttattga gagacgtgag catattggtg gtaatattta
     4981 cactgaaaat gttgaaggaa tacatgttca caagtatggc gctcatatat ttcatacgaa
     5041 tgataaaata atttgggatt atgtcaattc attcgtggaa tttaatcgct ttactaactc
     5101 acctttagca aattacaaag gtgcattata taatctccca tttaatatga atacatttta
     5161 tcaattatgg ggagtgcgga cgcctgatga agcaattaag aaaattaatg aacaaaaaca
     5221 agaaatcaat gctaaaacac cgtctaatct tgaggagcaa gcaatatcat tagttggtag
     5281 agatatatat gagaggttca ttaaaggtta tacagaaaaa cagtgggggc gaaaagctac
     5341 agaacttccc gcttttatta ttaaacgcat accagtaagg tttacctttg ataataacta
     5401 tttctctgat cgctatcaag gcattccgat cggtggatat tctggattag tagaaaagat
     5461 gcttgatggt gttgatgttc atttaaatgt tgattatcta gaaaaccgtg attattttga
     5521 ctctattgct gataggataa tttacacggg accaatcgat gccttttata attattgctt
     5581 tggatatctt gaatatcgga cgttattatt taaaacagaa attataaaac ggccaaactt
     5641 ccaaggtaat gcggttatca actatacaga acgcgatatt ccgtttacgc gtataattga
     5701 acataagcat tttgattata tagattcgca acttacagtt ataacaaaag agtttcccgg
     5761 cgaatggaaa cctggtgatg aaccgtacta tccagtaaat gatgacaaaa acatgacgat
     5821 ttataagaaa tatcgggaat tgtctaagaa aaataccaag tttgtatttg gaggcagatt
     5881 ggctgagtat aagtattatg atatgcatca ggttattaga tcagctttaa atcaagtaaa
     5941 attggaaata aattgatatg ctgtattgta taatagtaag ccataatcat gaacaacatg
     6001 tcaaaaatct actaagtttt gctgattata acccctgctc agatgtaaaa atcttggtaa
     6061 aagacaacat cggaaatgat gagttaaaga gagtctgtga aaaatatgag gttgaatatt
     6121 tttgtaacta taaaaaaatg gggtttggtc aaaataataa tgaaatggta agcaaaataa
     6181 ttaataaaca taaaataaac gataatttgg attatgtttt atttcttaat cctgacgttt
     6241 taattaagca tgaagtattg gttagttttt ataagtacat tatttctaaa aatataaagg
     6301 caagtacaat tgaccttttt aaaaatgaag attttactaa acgcgatgag tttatcagga
     6361 aatttcctac tcttactgat tttgtaagtt cgatcatatt taacatcaac aagacaaaaa
     6421 ttgacaggtc aaaaattatt gaaccatgtg aaatcgattg gtgtgctggt tcatttttat
     6481 gtattaattt ggctattttt cgcgatataa tgggattcga tgaacgctac tatatgtatt
     6541 gtgaagacat agatctttgt tatagattaa aattaaaaaa aatcaaatta gtttatctcc
     6601 ctcagtttaa agcaatacat ttagcaatgc atgagaatcg taagatttta tctaaggctt
     6661 tccgctggca tgtaagtaat gcaatatatt ttattattaa aaaatctatt ttcaaaagct
     6721 ttgattctta ttccaattca gtaaaaacca tcttcagagg atgaaatgaa agtattacat
     6781 gtagctacgg ggtttccttt tttatatgtt ggtggtatta caaactatgt cagatctcta
     6841 gttatttctc aacgtaatgc cggacacgaa gttcatgtct tgtctggtgg atgttcatca
     6901 gaaaatcttg agtatgtaac taatatttat tcaaaaaaaa ttaaaccttt ctcactaaag
     6961 ctgagcattg aagatgaaaa atatgcaaaa gaagtattaa atatatgccg agagtatgat
     7021 atcgtccatt ttcatatggt gttagacttt ccttcttcat tattaagtcg cttatcaaaa
     7081 gagattccca aatatgtagt gagcctgcat gactactttt tgatttgtcc tcgtatattt
     7141 atgagtgatt ataaaaacaa catttgtcat gaggtgaata ttgataagtg taatgtctgc
     7201 tgtggttatt ttgattctat tgatattgca cgtaaaataa gttctaaatt atctattcca
     7261 ctaccacgaa ttaaaactaa tgaacctgca agaagaattg aaaatgttaa aacatttcta
     7321 gagggtgcaa gtttattgct gcctgtttct gaaaaagtta aagagatata cagtaaactc
     7381 gtacctaatg cttcttacaa ggttcttcat attggtaata tcactgcaac ggaattgccc
     7441 ttgcctaaag aagaaaatgg aaagataatt attggtatat taggaactct ttcttatatt
     7501 aaggggagtg atattatttt gaaaataata aaacagacta ccaataaaaa tattgaattt
     7561 cacttctatg gaagagctga taagaaatgg ctgactagat tagaaaaata tggacttata
     7621 aacaagggga catatgtaca agaaaatttg aaagaaataa tatctactat caatttaggc
     7681 ctcgtcgttc cgatatggga agataatgcc cctcaggttg ttatggagtt tctcaaccta
     7741 aaaactcctg ttttagcgac aagtagaggt ggtataccag atttcataca acatttggta
     7801 aatggatatc tttttgatcc ggatgatcaa tgctccttcc aggcgtttat tgcttggata
     7861 aataatctaa atagaaaaac tatagtacag ttgaatgctg gtgtgaaaaa gcttaaaaca
     7921 cctgagcaac atgagaaaga aatcgatagg gtttatcatg ttatcaaaaa tatataaata
     7981 tggactggta gcttcaataa taattttttt ttctaaatta aagaactcta ttataaattc
     8041 atcatataaa cggcgctttg gtatcaaaaa tatatcaatt gggccggggg gatttatatc
     8101 aaactcttcc agaatatatt tttcagataa cttttatgct gggacaaatt tatggattga
     8161 gaatgtaaaa aacaaaggaa taattgtaat aggctctaat tgtaggttta gtaataatgt
     8221 tcatatagcg tgcataaacc gaataacaat tggcaaaggg gtgctaatag gtagtaatgt
     8281 attaattacg gatcatagtc atgggaaact tgtcgctgaa gaaaaattta ttagccctta
     8341 tgagcgtact ctcttttcta agggagaggt cattattggt gacaatgtat ggatatgcga
     8401 tggtgttatg atcttgccag gagttacgat tggctctgga gctattatag ctgctaattc
     8461 cgttgttgtt agcaacatac catcgtttac tgttgcggca ggaacacctg ctaaggttat
     8521 taaatgcatt ggggcgtgag aaccacttca ataatactta ttttattaac tgtttttttc
     8581 cctggaattg tgctttgggg aggaaatttt aaaatagagt tgattgtatt atgtattatg
     8641 tcattatgct taatattaag gataaatcag gtactacttt gcataaaacc tatttctatt
     8701 attatattct cttatatgct ttatgtattg ttgggtggaa ctggtttatt ttctggaaca
     8761 tttgattttt tttcgttaag aaatattctt tatcccttta ttaaagcatt ggttttagtg
     8821 atttttctgt ttttattttt gttaaaaaaa acacaaacac ccttgtatga tattaatagg
     8881 cttattttat tatgtttctt tatagattca attttcaaag ttatagaaat aatgtttcct
     8941 tcagttgtta gtgcttggct aaatataatt agtatagatg attggtggaa agatacccta
     9001 ggtatgagaa atcttgggtt cagaggtgtg tcgatatacg attactcagt agcagctacg
     9061 ttggctgttt taattgattt ctctttacga agcagaaaaa aaataaattt tataatttac
     9121 ccattagtat tactctctgc tttatcatcc ggtagaacag gtttagtagt tatatttata
     9181 tttacattgg tttattttta tcgttatgca cctaaaatta taatatcagg ttttatattt
     9241 ataatactaa taatgtattt gtcactattt cactctaatg agcttataca taatcagcaa
     9301 ctatcatgga tgttcgagcc tttgttaaac attcttaatg gaaatattga gaccgagtcg
     9361 acagatgatc ttttagagaa tcatctgttt atgccagata atatcttagg atatggtcta
     9421 tgggggcaat atggagatat gcttggaagc actattagag gatcagactc tggttatatt
     9481 ctgattatcc tatatggtgg ttgggtaggt tttattttct ttcttatttg ttcattttcc
     9541 tatttattgt atgcatatat taaaagtcag gataaaaaat tatctattgc aatatttttt
     9601 acttttatgc tgattatgat aaaaggtcca ataatttata gcgattataa tgcattcatt
     9661 ttaatgtctt tattgattgc accatcgttc tatatttttt ataaggctaa agaaaatgag
     9721 taacaattat tatattgttt gtcttgtagt aatatataaa gaaaaaatct tcgcgtctgg
     9781 aactattaga tctctccttc attctttttc tgaattaaaa gacactaata aaaaaatact
     9841 cgttaagtta tgggataact cccccgactc acagagttcc actgacttaa atagacttaa
     9901 agaattgttt ttacaaaatg atatattatt aagttatcag catcaaaaga ataattatcc
     9961 gttatcgatt gtgtataatc atttttttca agaatttata catcaagctc gctttctaat
    10021 gttgttggat caagattcaa tatttggtcc tgagtatttt caagagtttt ataatgctga
    10081 gaagagaagt aatgtagatc tatttttgcc caaaatcatg cataaaggca gaataatcag
    10141 tcctacaaag atatattatg caaaaggttt ttattacaag gaaattgcat ctggttttat
    10201 ttcatcaaaa aaactaggtg ctataaatag tggtatgatt atttcttcta aatatcttat
    10261 ccgaacgcgt ttttcttatg atataagact tggcaattac tgtacagatg acttttttat
    10321 gaagaaattc cgggaaaata aaggtattgc atatattcta gattattcat tcgatcatga
    10381 cttaacattg tccaccttaa atgataatag tgactccttg agaagacgct atgaggaaat
    10441 gatcaaaggt aaaaggttag tatatagcaa taatatattt gagatgatat ttatcaggct
    10501 atatattatt ctaaatagat gttatatatc attgaaatat aaagatctga aatatttgaa
    10561 aggattttat gaataagtta atcatttcta tcccacaatt taatctctct ggtggaaatt
    10621 tggtgtcatt ggagctaggt cttttcctaa agaaaaaagg attagatgta tacgggcatt
    10681 ctggatttag gttaaaaaaa attgatgaga ttgagcttaa tattcctaaa agaggtctat
    10741 gtaacactat cataaatata ataagtttct tttgttcttc tttttattct ttattttcta
    10801 aatattatat agctacacac catttgacgt cattatttaa ttttataaaa cctgcaaaat
    10861 tctcacttat ccaagacatt gaatcattgt tttaccctaa gcgtctgagt tggtttggaa
    10921 ctatattatg gagaaattat ctaaaatctg acactctgtt atatacaagt aagtatcttg
    10981 aacaaagaat acataacaat aataacccta aaacaattgg ctttccattt attaaaacat
    11041 ctactaactt tagtcttaca ggtgagaaaa tttatgacgc tttaatgatt ttacgtgatg
    11101 gagattataa aaattataat ctaactttaa gtgagtttta tagactgaat aacaacggat
    11161 ttacagttgc attagtaaat gcgtctcgaa atagcttacc atttaatcca ttgatattct
    11221 ctaatttgtg tagggatgat tttattaaag tgcttatgat gtcaaaaagg tttatatgtt
    11281 tgtcaaagtg ggaaggactt ggtcttccta atttagaagc atattcctta ggattagaaa
    11341 tcatatcaac aaaaatacct tcagcacaaa taatagagga gatagatcct aatgctatta
    11401 ctattataga tagtattgaa caagttgaaa aggccattat cagtaataga gttcaaatct
    11461 ctgataataa aatgtactta agatccgaat cattgactga cttggattcg ctgtggaaag
    11521 aacacatatt cagcacaatc cgatattata tgggccaata gtgttcttaa gtctataaaa
    11581 tcctattaac tatgaatcaa caaaatgttg ataaatataa tgaatacatt caaactgcac
    11641 tttgttgtgc gttgcgccac tgataggagt aaacaatgtc aaagcaacag atcggcgtag
    11701 tcggtatggc agtg
//

</pre>