<pre>LOCUS       KJ755544               10560 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain S 239 serotype O131:K-:H26 O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ755544
VERSION     KJ755544.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10560)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10560)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Liu,Y. and Debroy,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: Average 50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S 239"
                     /serotype="O131:K-:H26"
                     /db_xref="taxon:562"
     misc_feature    1..10560
                     /note="O-antigen gene cluster"
     gene            114..734
                     /gene="nnaD"
     CDS             114..734
                     /gene="nnaD"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="AIG62396.1"
                     /translation="MKKKLIIIGAGGFAKAVIDSLDYDEYVLEGFIDSLKSGMHQGYP
                     IVGHSLSDIRLPTDYYYFIAIGNPDDRALWLRQIQDLNLDTINVIDRTAIVSTRSSIG
                     TCIYIGKMAIVNCDSQLEDGVVINTRALIEHGNYISYCTNVSTNVVLNGDVYVGEKSF
                     IGSCSVVNGQIKIGKSTIIGSGSVVIRDIPDNVVVAGTPTRLIRER"
     gene            736..1776
                     /gene="nnaB"
     CDS             736..1776
                     /gene="nnaB"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl neuramic acid synthetase"
                     /protein_id="AIG62397.1"
                     /translation="MSKVYIVAEIGCNHNGDYNLAKKMVEEAKIAGVDAVKFQTFKAE
                     QLISKYAPKAEYQIKVTGNEESQLEMTRKLELPYEEFIELEEYAKELGLDVFSTPFDF
                     ESIDFLASRNQKMWKIPSGEILNLPYLEKICRLPIEDKKIVLSTGMATLDEIRICLDV
                     LNKNGVLPENITILHCNTEYPTPFEDVNLNSIHGLKKEFKEYNIGFSDHSPGFFAGIA
                     AVPYGITFIEKHFTLDKNFEGPDHKASVSPDELRLLCQGIREVEKSLGSYIKLVTKSE
                     RKNKIVARKSIVAKRNIKKGDIFTIDNITTKRPGNGISPMYWYELLGKKAERDFDEDQ
                     LIIHSGFVTQEV"
     gene            1779..3041
                     /gene="nnaC"
     CDS             1779..3041
                     /gene="nnaC"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="AIG62398.1"
                     /translation="MANRLAIIPARSGSKGLANKNILMLMGKPLIAYTIEAAVNSKCF
                     SKVIVSTDSIEYKYIAEQYGAEVLIRNERLASDTATSFMVIEDVLKKYRGFDYFVLLQ
                     PTSPFRNTKHIQESIDLFESKEGINFLVSVTESNKNSELIKPLDELHRLTNFGSDFSN
                     YRRQNVKEYSPNGAIFIGRNDSYLKKKHFFGSDSIAYIMNKEDSIDIDDRIDFEIAIA
                     LATKKLKQELLIKKIKHRIAEKFSKINSGAPITLIGHSLFDNWEIDSLAGKKVNNYGI
                     AGINSEQYYQFVLKNGIISELGDVVFLYSGTNDIVLENWTVEYTIYWTTKIIEELKKI
                     NSNVRVYLLAVPPVNGRVDRNNYIINELNHLLFRHVKCLSNVCWVPLSKAFYDEFGNL
                     KHSFTSDGLHFTKKAYEQLQRDLEEVMK"
     gene            3038..4183
                     /gene="phaR"
     CDS             3038..4183
                     /gene="phaR"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor"
                     /protein_id="AIG62399.1"
                     /translation="MKKILYVTGSRAEYGIMKRLLLQLDKEDDIKLTIIATGMHCDEK
                     YGLTYKFIENDGLTIERLINIDIDNSSNGGILRTMAKCQTEFGDYFENNKFDAVMILG
                     DRYEILSVAIAAAIHNLPIIHLHGGEQTLGNYDEFIRHAITKMSNLHLVSTDEYRNRI
                     IQMGEHPDSVHNIGALGAENVIKMKLPTIIELENKFGSLARPYFMVVFHPETLSEESP
                     ITQVEELLGALDLLSPSFDFIFIGSNSDTKADEIAIRLKKYCQQNKNRFIPSIKPEEY
                     LALNKYSQGLIGNSSSGLIEIPSLKIPTINIGNRQKGRVRGDSVIDTDCKRYNIYHSI
                     RLSQTRDFKNKISSSENPYYRDNALYHALKIIKSFIINNRKDVKKFL"
     gene            4217..5485
                     /gene="wzx"
     CDS             4217..5485
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62400.1"
                     /translation="MSVYKNSSIYLISNIFNALIPFILLPILTRNLTPYEYGQIAMFQ
                     TLVSGLASLTGLNTVGAANRRYYDITSKSGLAVYNTNCFYILILSCAALLLLSLLLSD
                     LLSSLLTIPNNWVYLSVVISGATFIIQFRLGQWQIREKAFWFGILQISQSVIVSVLTI
                     IFLICMHQGASSRVNSLFMVSIVYALVSLYSLYKSKLIMLTKINLLDVRDALYFGVPL
                     IPHILGIFCLSALDRVLINGVLGASEAGIYMLAAQLSLGMMVFFDALNKALVPWLFNA
                     LSLNDVNKINSLIRFTYLFFIIVAILGGLSFWIGPLVVELVAGNEYLNAKNIIGWLCL
                     GQAFNGMYLMVTNYLFYAKCTGRLSIVTIFSGGVNIVLLLVLMKFLGITGVAISFSIS
                     MFIRFLATWWLAAKVCGFSWRMSIRRIIFG"
     CDS             5525..6466
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 52"
                     /protein_id="AIG62401.1"
                     /translation="MNLFICRTIFQVYYAGILIKNKNIKDVMLAYLSEGLSDREKNVL
                     SCCGINRIVVIEGSNPYKRLLKQIWFLNYLKRITKKQSVSLYFSSIDDPFIQAIISKN
                     KFGDVYTFDDGTANYIQNSFFYVETKRNIFVRIKYYLIGNRIKSLCEVKKLIKIHYST
                     NHLKNIIDNVEYIPLYGKSNHSDLSVESKKHEVVNLYLCPNFDEIYIDAEKVKRKFIS
                     TLTKHDIIIPHPRDKCMWDAEANYKIRNDTMAEIVIDEYLSKGCLINLFGIANSTQYH
                     YLQNNKVVNHVIDIGEIKPKFKEVIPKQIDCFKKLTK"
     gene            6488..7792
                     /gene="wzy"
     CDS             6488..7792
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG62402.1"
                     /translation="MGFIKVFNLKNSIVDDRYNLLWLFAGLLVVFVLGVLLFPLAGFF
                     LFFFVAFLIANSSKFKLKRMVFFVLYFMLVMCIIINENSIQRFIYREDDFTTYYNNYL
                     ELLNGNYEFLFQFGGGAEIGLPALNYIFSFFIGNPFPYFLQMTYIGMYIVMLYYLVSI
                     DRYFGNRDKSNKLDLLLWATLFLKITAMLTIERQAVASFFILYAISDIRRKYLWLFIG
                     CLFHLSTPVVYLAVRFVLNTKTNKKVLVSCIALILFVVFSHQLLSVINHILPNDKVGY
                     VLYYINNGDFIKNELVKSIKQVSYVIPLLLLDFAMRLQGYRWKLSSSLQLFVYSMLIL
                     SFLPGVPTRIFMPIVFILYGFYYYDFICLFRIKTRVIIFLIITSFFSVYKFFLPGYYY
                     RYPIANIYPGYYISSFFDKYGYVERYSLPYSSDININNDDKL"
     CDS             7789..8649
                     /codon_start=1
                     /transl_table=11
                     /product="core-2/I-branching enzyme"
                     /protein_id="AIG62403.1"
                     /translation="MKIIYLLQCHKSFEQIKLLLESLELSREDFVIIHVDAKNINLRH
                     RISSYYFDSDNVFVVNDPVVVNWSGFSQIKATLKMIGYVYRKKINFDYCCLLSGEDIV
                     LDVFRFKKYLSLSGQKSFLEFRDDRERYTWRINRFNLFRDNRFSRKYLLRLISFLLIK
                     IQLKGNIRRSNFIDDDIFLGSQWFVFSKRHLDLIYNLVDDNYINKFRYTSCCDEHFFQ
                     MFFKKFVHQEEYETYNLRYAHFPTGKNSPKYLTLLDLKNIKGKDKVFIARKVSYDVLY
                     DYMKNKGSGG"
     gene            9114..9920
                     /gene="amsE"
     CDS             9114..9920
                     /gene="amsE"
                     /codon_start=1
                     /transl_table=11
                     /product="amylovoran biosynthesis protein"
                     /protein_id="AIG62404.1"
                     /translation="MKFTVLLSLYNKEDKCYLYDCLDSIRLNTRTPEQVVIVYDGPIG
                     EELNSVVTYFSNLLPIEIVRIPHNVGLGQALNRGLEFCRNEIVFRMDTDDKCDRLRFE
                     KQLDMLKIHPDIVLMGGAVEEFDETMTKSLGVRYTVESHEQIKSYVRKRNPFNHMTIM
                     FKKSIIQSLGGYEHHHLMEDYNLWLRVIAAGYKSYNIPQVLVYVRAGRGMLGRRRGIL
                     YVFSEIKLAKLKYKLKTDNIYGVVKYSFIRILPRLLPVFILRHLYAMLRK"
ORIGIN      
        1 aaaatatatc tctacagtgt actggtagct gttaagccag gggcggtagc gtgtctgata
       61 gtttcttcag tggaagctaa attcaaatac tgaacctcac ttatggtgcg taaatgaaga
      121 aaaaattgat cattatcgga gctggtgggt ttgctaaagc ggtaatagat agtttggatt
      181 atgatgaata tgttctcgag gggtttattg attctcttaa atcaggaatg catcaaggat
      241 atcctatagt tggtcattca ttaagtgata ttcgtttgcc aactgattac tactatttta
      301 ttgccattgg taatcctgac gatagagcat tgtggttaag acaaatacaa gatctaaatc
      361 ttgacaccat aaatgttatt gatagaacag caattgtttc aacgagatcc tctatcggaa
      421 catgtattta tattggtaaa atggctatag taaattgtga ttcacaatta gaagatggtg
      481 ttgttattaa taccagagcc ttgatagagc atgggaatta tatctcatat tgtacaaatg
      541 tttctactaa tgtagtcctt aatggtgatg tatatgtcgg ggagaaaagt tttataggta
      601 gttgtagtgt tgtcaatgga caaataaaaa taggcaagtc aactattatc ggctcggggt
      661 cagtggttat tcgtgatatt cctgataatg tagttgttgc aggtacacca acaagattaa
      721 taagagaaag gtaaaatgag taaagtatat attgtggcag agattggttg taatcataat
      781 ggtgattata atcttgcaaa gaaaatggtt gaagaggcaa aaatcgcagg ggttgatgca
      841 gttaaattcc agacatttaa agcagaacaa ttaatttcga agtatgcacc taaggcagaa
      901 tatcagataa aagttacagg gaatgaggaa tcccagttag aaatgacaag aaagctcgaa
      961 ttaccttatg aagagtttat agagcttgaa gagtatgcaa aagaattagg gcttgatgtt
     1021 ttttcaactc catttgattt tgaatcgatt gattttttgg catcacgaaa ccaaaagatg
     1081 tggaaaattc cttcaggaga aatacttaat ttaccgtatc tggaaaagat ttgtcgatta
     1141 ccaatagaag ataaaaaaat tgttctttcg acaggaatgg cgacgcttga tgaaataaga
     1201 atatgtttag atgttcttaa taaaaatgga gtgttgccag aaaatataac aatattacat
     1261 tgtaatactg aatatcctac accatttgag gatgtaaatt taaactcaat tcatgggtta
     1321 aagaaagaat ttaaagaata taatattgga ttttcagatc attctcctgg atttttcgct
     1381 ggaatagctg cagtgcccta tgggattacc tttattgaga aacattttac gctggacaag
     1441 aattttgaag ggccagatca taaagcatca gtaagtcctg atgaattacg cctcctatgc
     1501 caagggattc gtgaagttga gaaatctctt ggtagctata ttaaattagt tactaagtct
     1561 gaacgaaaaa ataaaatcgt agctagaaag tctatcgtag ctaagcggaa tataaaaaaa
     1621 ggtgatatat ttacaattga taatattacg acaaaacgac caggtaatgg aataagtcct
     1681 atgtattggt atgaactatt gggtaaaaaa gccgagaggg atttcgatga agatcaactt
     1741 atcatccatt caggttttgt tactcaagag gtataataat ggcaaatcga ttggctatta
     1801 ttccggcacg ttctggttcg aaaggattag ctaataaaaa tatactaatg ttgatgggga
     1861 aacctttgat tgcttataca atagaagctg ccgtaaactc taaatgtttc tctaaggtaa
     1921 tcgtttcaac agattctata gaatataaat atattgccga gcagtacggt gctgaagttt
     1981 tgatacgtaa tgagcggctt gcaagtgata ctgcaacgtc ttttatggtg atagaagatg
     2041 ttttaaaaaa atatagaggt tttgattatt tcgttttgtt gcaacctact tcaccatttc
     2101 gaaataccaa gcatatacaa gaatctattg acttatttga gtcgaaggag ggaataaatt
     2161 tcttagtgtc ggtaacagag agtaataaga attcagaatt aattaaacca ttagatgaac
     2221 tgcacaggtt aacaaatttt ggaagtgatt tcagtaacta tcgacgacaa aatgttaaag
     2281 aatattcgcc caatggagca atttttattg ggcgtaatga tagctattta aagaaaaaac
     2341 atttttttgg ttctgacagt atcgcttata taatgaataa agaagattct attgatattg
     2401 atgaccgaat tgattttgaa atagcgatcg cgttagcaac aaaaaaactg aaacaggaac
     2461 tgctaataaa aaaaattaag catcgtattg ctgaaaaatt tagcaagata aattctggtg
     2521 cacctattac attaatcggt cattctctat ttgataattg ggaaattgat agtttagcag
     2581 ggaaaaaagt taataactat ggtatcgcag gaataaatag cgagcaatac tatcagtttg
     2641 ttttaaaaaa tggaattata tcagagcttg gagatgttgt ttttttatac tcgggtacaa
     2701 atgatattgt actcgagaat tggactgtag agtatactat ttattggaca accaaaataa
     2761 ttgaagagtt aaagaagata aattcgaatg ttagagttta tctcttagct gtacctccag
     2821 taaatggacg agttgatcgt aacaactata ttattaatga gttgaatcat ttgttgttcc
     2881 gacatgtaaa atgcttaagt aatgtatgtt gggtaccact gtcaaaagca ttttatgatg
     2941 aatttggtaa ccttaagcat tcatttacta gcgacggatt gcattttaca aaaaaagcat
     3001 atgagcaact tcaacgagat ttagaagagg taatgaaatg aagaaaatat tatatgttac
     3061 aggctctcgt gctgaatacg gtataatgaa aaggctatta ctacaattag ataaagaaga
     3121 tgatattaag ctaacaatta ttgctacggg aatgcattgt gatgagaaat atggattgac
     3181 gtataaattt attgaaaatg atggtttgac gatagagagg ttgattaata tcgatataga
     3241 taatagctct aatgggggta ttcttagaac aatggctaaa tgccaaacag aatttggtga
     3301 ttactttgaa aataataaat ttgatgctgt catgatatta ggtgatcgat atgagatttt
     3361 atcagtagcc attgccgccg caattcataa tttaccaatt atccatttac atgggggaga
     3421 gcaaacatta gggaattacg atgaatttat acgccacgca atcacaaaaa tgagcaatct
     3481 tcatctggta tcaaccgatg aataccgtaa tagaattatc caaatggggg aacatcctga
     3541 ctcggtccat aatataggag ctcttggagc tgaaaacgtt attaagatga aattgccaac
     3601 tatcattgag cttgaaaata aatttggctc tcttgctcgc ccctatttta tggttgtttt
     3661 tcatcctgaa acactaagtg aagaatcacc aattacgcaa gtagaagaac tacttggagc
     3721 attggattta ttaagtcctt cctttgattt tatttttatt ggttcaaact ctgatacaaa
     3781 agctgacgag atcgctatta gattaaaaaa gtattgccaa cagaataaaa acagattcat
     3841 tccatcaatt aaacctgaag agtatctcgc attaaataaa tattcgcaag gattaattgg
     3901 aaactcatca tctggcctaa tagagatacc ctctttgaaa atcccgacta taaatatagg
     3961 aaatagacaa aaaggacgcg tccgtggaga ttctgtgatt gataccgatt gtaaaagata
     4021 taatatctat cattcaatac gtttaagtca aacacgtgat tttaaaaata aaatatcttc
     4081 gtctgaaaat ccttattatc gtgataacgc tttatatcat gccttgaaga taattaagag
     4141 ttttataata aataatagga aggatgtaaa aaaattttta tgatataaag ataaatgcgg
     4201 acaatgagtc aaacgtatga gtgtatataa aaactcaagt atttacttga tatcaaatat
     4261 attcaatgca ttaattccat ttatattgct tcctattttg acaagaaacc taactcctta
     4321 tgaatacggg caaatagcca tgttccaaac attagtaagt ggattggctt ctttaacagg
     4381 tttaaataca gttggcgctg caaatcggcg ttattatgat ataacctcaa agagtggttt
     4441 ggctgtttac aatactaact gtttttatat tttaatttta agttgtgctg cgttacttct
     4501 tttatctcta ttgctatctg atctattgag ttcattgttg acaattccaa ataattgggt
     4561 atacctatcg gtcgtgattt ctggggcaac atttataatt caatttcgtt tagggcaatg
     4621 gcagattcgt gagaaggctt tttggtttgg aatattacag attagtcaga gtgtcattgt
     4681 atccgtgtta accattattt ttttaatttg tatgcatcaa ggagcatcta gtagagtaaa
     4741 ttctttattt atggtaagca tagtatatgc gctggttagt ctttatagtt tatataaaag
     4801 caaattaatc atgcttacaa aaattaatct tttggatgtc agagatgctc tatattttgg
     4861 agtccctttg ataccacata tcttaggaat tttttgttta agcgcattgg atagggtttt
     4921 aattaatgga gtattaggtg ctagtgaggc tgggatatat atgttggcgg ctcagctaag
     4981 tttggggatg atggtttttt ttgatgcgtt aaataaagca ttagtgcctt ggttgtttaa
     5041 tgctctatct ttaaatgacg taaacaaaat aaatagtttg ataagattta catatctgtt
     5101 ttttattatt gttgcaattt tgggggggct ctctttttgg attggccctc tcgttgttga
     5161 gttagttgcc ggaaatgaat atctaaatgc taaaaatatt attggctggc tttgtttagg
     5221 gcaggctttt aatggtatgt accttatggt tacaaattat ttattttatg caaaatgtac
     5281 gggaaggtta tcaattgtaa ccattttcag tgggggggta aatatagttt tattgttggt
     5341 gttgatgaag tttttaggga taactggagt agctatctca ttttcaatat caatgtttat
     5401 tcgattttta gctacctggt ggttagcagc gaaggtatgt ggattttcat ggcgaatgtc
     5461 aattaggaga attatttttg gatgaggctt tttgtgtaaa tgtattgtaa ggagaaatat
     5521 aaaaatgaac ttatttatat gccggaccat atttcaggta tattatgcag ggatcttaat
     5581 taaaaataaa aacattaaag atgttatgct cgcttattta tctgaaggat tatctgatag
     5641 agaaaaaaat gtattatcat gttgtggtat taaccgtata gtagtcattg aaggttcgaa
     5701 tccgtataag cggcttttaa aacaaatttg gtttttgaat tatttgaaaa gaataacaaa
     5761 aaaacaatct gtaagtcttt acttttcaag tattgatgat ccattcattc aagcaataat
     5821 ttcgaaaaat aagtttggag atgtatatac gtttgatgat ggaaccgcta attatataca
     5881 aaattcattt ttctatgtcg agacaaaaag aaatatattt gtaagaatca agtattatct
     5941 tattggaaat agaataaaaa gtctgtgtga ggttaaaaaa ctaattaaaa tacactactc
     6001 aacgaatcat ttaaaaaaca tcattgataa tgtggaatat atccctttat atggtaaaag
     6061 caaccattcc gatttgtcgg tggaatcgaa aaaacatgag gtggttaatt tatacttgtg
     6121 ccctaatttt gatgagattt atattgatgc tgaaaaggta aagagaaaat ttatatctac
     6181 acttactaaa catgatatca taatacctca tccaagagat aaatgtatgt gggatgctga
     6241 agcgaattat aaaataagaa atgacacaat ggcagaaata gtgatagatg aatatctgtc
     6301 taaagggtgt ttgataaatc tatttggaat tgcaaactca actcaatatc attacctcca
     6361 gaataataag gttgtaaatc atgtgattga cattggagaa ataaaaccaa aatttaaaga
     6421 agtgattcca aagcaaattg attgtttcaa aaagttgaca aagtaaaata attgcattta
     6481 tataaatgtg ggttttataa aggtatttaa tttgaaaaat tcaattgttg atgataggta
     6541 taacttattg tggctatttg caggcttgtt agtggttttt gtcttgggag tactattatt
     6601 tccacttgct ggattttttt tatttttttt tgttgcgttt ttgattgcga attccagcaa
     6661 atttaaacta aaaagaatgg tgttttttgt tttatacttc atgttagtaa tgtgtatcat
     6721 tattaatgag aatagtattc agcgattcat ttacagagaa gacgatttta cgacatatta
     6781 taacaattat ttagagcttc tgaatggaaa ttatgagttt ttgtttcaat ttggaggggg
     6841 ggcagaaatt ggattgcctg ccctcaatta tatattttca ttttttatag ggaatccctt
     6901 tccttatttt ctgcaaatga catatattgg tatgtatatt gtcatgctat attatttagt
     6961 atccatcgac cgatactttg ggaatagaga taagagtaat aaattagatt tactgctgtg
     7021 ggcaactctt tttctgaaaa taacagcaat gttaactata gagcgacagg cggttgcatc
     7081 tttttttata ttatatgcta tttcagatat taggcgaaag tatttatggt tgtttatagg
     7141 atgcctcttt catctcagta ccccggttgt atatctggct gttaggtttg tattaaatac
     7201 aaaaacgaat aagaaagtat tagtctcatg cattgcttta attttgtttg ttgttttttc
     7261 acatcaactt ttatctgtaa taaatcatat tctgccaaat gataaagtag ggtatgtttt
     7321 gtattatatt aacaatggtg attttattaa aaatgaactt gtaaaatcaa ttaagcaagt
     7381 tagctatgta ataccgttat tacttttgga tttcgctatg agacttcaag gatacaggtg
     7441 gaagttaagt tcatcactac agctctttgt ctatagtatg ctgattttat catttctgcc
     7501 cggggtgcct acaagaatat ttatgccaat tgtctttatt ttgtatggtt tttattatta
     7561 tgactttatt tgtttgtttc gcataaaaac gcgtgtcata atattcctca ttatcacatc
     7621 ctttttttca gtctataaat tctttttacc tggttattat tacagatacc caatagctaa
     7681 tatctatccc ggatattata tatcatcttt ttttgataag tatggatatg tagaacgata
     7741 ttctcttcct tattcatctg atataaatat taataatgac gataaactat gaaaataata
     7801 tatttattac agtgccataa gtcatttgag caaataaaac ttcttttgga atcacttgag
     7861 ttatctcggg aggattttgt tataatacac gttgatgcta aaaatataaa tctgaggcat
     7921 cgaattagtt cctattattt tgatagtgat aatgtttttg ttgttaatga ccctgttgtg
     7981 gtgaactggt ctggtttttc gcagataaaa gcaacattaa aaatgattgg ttatgtatat
     8041 aggaagaaaa ttaattttga ctattgttgt ttattgagtg gtgaggatat tgttcttgat
     8101 gtgttcagat ttaaaaagta tctttcatta tctgggcaaa agtcattttt ggaatttaga
     8161 gatgacaggg aacgctatac atggagaatt aataggttta atttattcag ggataataga
     8221 ttttcacgaa agtatttgtt gcgtttgatt tcattcttac taataaaaat tcagttaaaa
     8281 ggtaatataa ggcggagtaa tttcattgat gatgatattt ttttgggaag tcaatggttt
     8341 gtttttagta aaagacattt ggatttgatt tataatcttg tagatgacaa ctatattaat
     8401 aagtttcgtt atacctcatg ttgtgatgag catttttttc aaatgttttt taagaaattt
     8461 gttcatcagg aggaatatga aacttataac ttaagatatg cacattttcc gactggaaag
     8521 aatagtccga agtatttaac acttttagat ttgaaaaata taaagggtaa ggacaaggtg
     8581 tttattgcta gaaaagtatc gtatgatgtt ttatacgatt acatgaaaaa taaagggagt
     8641 ggtggatagt atataatcaa aatgtatttg cctttttttg aaactcagct ttgttgtttt
     8701 tataggtcaa tgtaaacatg tttttctgta tatattaata tagcaagagg taccacgctg
     8761 atattctgat ttttcggagt gctgtttatt atttgatgtt gttttttata gtcatatgat
     8821 caaatagttc tttctatata catgatgaga gtaaaataga atattggaag tataagggta
     8881 gcatggtttt attttgcttg tatatatatt tgcgattttc gtaagataca tcgtgattct
     8941 tctaatttaa tagattgatt ttttcgtgga agtctttacg atatcattac tgggttttta
     9001 ttatgtctga attttgcaaa atccctgaga gataattata taatccacaa aaaaaagtaa
     9061 ttgagataaa gttttatatc taatttagaa tgtaaaatgt tggggatgta ataatgaaat
     9121 ttacagtttt actttctctg tataataaag aagataaatg ttatttatat gactgtttag
     9181 atagtattag gttgaatacc agaaccccag agcaagtagt gattgtctat gatggaccta
     9241 ttggagaaga gttgaattct gttgttacat atttttctaa tttgttgcct atagaaatag
     9301 tacgtattcc acataatgtt ggattggggc aggcgttgaa tcgtggtctg gagttttgtc
     9361 gaaatgaaat tgttttcaga atggatacag atgataaatg tgatcggttg agatttgaaa
     9421 aacagttgga tatgttaaaa attcatcctg atattgttct tatgggcggc gctgttgaag
     9481 aatttgatga aacaatgaca aaatcacttg gtgttagata tacagtggag tctcatgaac
     9541 aaataaagtc atatgtaagg aaacgtaacc cgtttaatca catgacaatt atgttcaaaa
     9601 aatcaataat tcaaagtttg ggtggatatg agcatcatca tttgatggag gattataatt
     9661 tatggctaag agtaattgct gcaggttata aaagttataa tattcctcag gtacttgttt
     9721 atgttagggc tggcaggggt atgttaggta gacgaagggg gattttgtat gtgtttagtg
     9781 aaattaaatt agcgaagcta aaatataagt taaaaacaga taatatatat ggggtggtta
     9841 agtattcatt tattcgtatt ttgccacgtc tacttcctgt ttttatatta agacacttgt
     9901 atgcaatgtt aagaaaatag attaaaacat acctgtgtta ttcgtaataa tgggtggtgg
     9961 ttggttactt tagtatgcta aatgttaacg atgaatttag caagtccgag actatggaat
    10021 tcttacagat attgtcgtta cataacaaat gaaaagaatt gcaacgaata aaaaagttaa
    10081 aaaattattc ttgtgactat catctctctc aataatggca gagaaaaagc tcgaatgatg
    10141 gtcagatatt atgtctgtct tgctgtgatt taatcgcaga gaaatttact atagtagtcc
    10201 gaaattacta gtacatagag aataagctgt ttgttgttgg agcagataaa tggagcagga
    10261 tgtctggacc ataagataag aataaggaac acaatagaat tattttcaga gataagtctc
    10321 atttctattg atatttagat gatcgataag gtatttaagg cgggattaag atataaatac
    10381 gaaggcaacc atagacagaa actatctcgt gtcaaccatt tataggaaat ggactcgcgt
    10441 aatcttgcct tggacatatt ttcgtgctta tattctggcc acaattctcg cggtaaccct
    10501 gacaggagta aacaaatgtc aaagcaacag atcggcgtcg tcggtatggc agttaagtgg
//

</pre>