<pre>LOCUS       EU296421               10990 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Escherichia coli serogroup O130 O-antigen gene cluster, complete
            sequence.
ACCESSION   EU296421
VERSION     EU296421.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10990)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 10990)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..10990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O130"
     misc_feature    1370..9543
                     /note="O-antigen gene cluster"
     gene            1370..2644
                     /gene="wzx"
     CDS             1370..2644
                     /gene="wzx"
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ACD37150.1"
                     /translation="MLDILRIQEKYFMKFTLKKTNIQNRNVVIYLISDLLAKALPFLF
                     IPVLTKYLTPEQYGNIALFNIGVETFLIIIIMGGNSYYKIEYPKFKDPILSLYIIIYN
                     VTLLFLVFFILSIVIWFFYERIYIIDVLPLILLCAYFQSLFYLYISYYQCNEKALVVG
                     MVNLFFSLSSSLLMILFLVEFKQAESSRYWSLLGGLIASVLLLGSILANKSNRKYKLV
                     NKIDFELIKFGVGVFPHAVSWWARSGMERLLIGWFLSVSTLGIYSLAMQLTSIMPLFC
                     NAINQALMPRIVRCLNDNKIEETKKILVKSSLIVAIACVFSSVVMPLFLGYVLNDRYH
                     EALEYLPYMILSFVFQGVIIIYTNVLYFYKQVKYLSVATFGTSCVHLLLSMLLIKINL
                     NVYSVIISSVITFLFASIVLVRKAYQIMSERY"
     gene            2645..3445
                     /gene="wffF"
     CDS             2645..3445
                     /gene="wffF"
                     /note="glycerol-3-phosphate cytidyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WffF"
                     /protein_id="ACD37151.1"
                     /translation="MILTKIKNRLIIFVSKLLYQSRAYYYRCKNRKKKKIFIYTDSRG
                     YEVSNLWNKKNPFSSYVGDLVKEYNVEYHICEYSSTTIIDFLYEYYGEGTRGKKYDYV
                     IAHVGLVDFSPRPRSMANDIIKSKSHKIKFFKWDLRMFDKYLEREISSEIYNGELLSN
                     LYDCEFLKNEIIPLLQNIPNLIYIGCNPIISNWRGNYWQDRPKDINRILMAYNSVMLT
                     ELKEKKVVDIKDWNECDIRNYTVDNIHLNREGYNKITLNLKQILTEIK"
     gene            3445..4644
                     /gene="wffG"
     CDS             3445..4644
                     /gene="wffG"
                     /note="glycerophosphotransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WffG"
                     /protein_id="ACD37152.1"
                     /translation="MKFIFTRLVIFLLSVFVPTKKGLFIFGSWFGKKYGDNTRALFEH
                     LSNIQPENVYWYTDNEKIASKIIASGNKCISGVNMKNIFLHLRAEAVFCNCSANSDLL
                     GGYINKRTKVFNLWHGTPMKKIGKDAVKSGLSHTRLGMSKPNLTLSLIKKILPSTFYN
                     FLKLDTYYLASSPEVSKILQGAMGVNEEKIIICGYPKLDKIFIESGYAEPYKILYAPT
                     YRGEYNSELDILTMFGFNIELADKVLKENKATLTIRLHPANKLPVAVINRINNTNTIS
                     IDNEDDIYESLGKYSLVITDYSSVYFDALAVGINVLIAPFGYKDYLENDRDLYFSLSE
                     LYPERMPIDWKDFFEHFSYFASNKREQFSSIRNKFYSFPYAPCSDKLLSLVYLKLGRE
                     YVNKKNY"
     gene            4622..5737
                     /gene="wzy"
     CDS             4622..5737
                     /gene="wzy"
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ACD37157.1"
                     /translation="MLTKRTTKNTSFLYFSLFCIYFSVSFSPVNPTYILGCGLILILF
                     MQVLLRMRIQKFTLITIVFAMMFLLEQLIGINIVSFYPEGPVYLTPLLFSYSILISAC
                     VIEGFQGLKSDSRLIIYKRVYNYAIIFLVIELFTRVLNYNKENNGFYSLKYSVPYFDS
                     NFTGLVILSLCSYLLYLKYVKGNERKIHRRILYFLLLATFSRGAIAALILTRIGLGSK
                     KRIRGKAFLIIAVAVVIFSMLTIEYIEQSQSYSSIDGSFNSKFYIISQALSFYESLPI
                     LVHWFGIGMGNTSQFIGIFAHNIFVTMILEMGIVGTVLFLLYVFYTLKLSNGYALFIW
                     VPIFVGGISLFSAFSPFAFIICALICCENMVDKKYEF"
     gene            5727..6665
                     /gene="wffH'"
     CDS             5727..6665
                     /gene="wffH'"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WffH'"
                     /protein_id="ACD37153.1"
                     /translation="MSFRDNPKVSVLIPAYNVAEWIEESLLSILGQSYENIEVIVVDD
                     CSTDGTYEIIRRVAENEPRMKVLRNERNMKIVDTLNYGLTHCTGDFVLRHDGDDVSEI
                     KRIEVQLKHLLENELDLVGTQMLPIGRSGRVIGAQSRLPLSHELIEKIANFSSPLTHI
                     WICKKEIYDKLGGYRKVPYAEDFDFLLRAIDSGFKCGNTSVALTKIRHRHGNTSDIAS
                     LSQRKGYFYALELHKQRKAQNYDSYNDVDAAKLIKHNKLVNYFHSLSTKCLHKAFQSN
                     NKLEMFFYTLLSAVMSFYNAHYIYQRIKVKMLLSNQ"
     gene            6736..7779
                     /gene="wffI"
     CDS             6736..7779
                     /gene="wffI"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WffI"
                     /protein_id="ACD37154.1"
                     /translation="MRILILIEDITLGGGTERVALTLATNLNADGINCDIFSLSKSNT
                     DTFYPSENINICYAKSKVGVFAKIEAIKYAKKNNMKLLVFSMGRLSVEIALLSRLFLF
                     RHFGCYEHISFSSFSGFIQKIKLFAYRLSHGVIFLTEHDRDIIRHKLSHVPVTSIENI
                     SPFRAIKKSNVGARKNIVLAVGRLTNQKNFSRLINLWSMVNRPDWSLIIAGDGPDRDF
                     LDKQINHLCLKNVEIVGAVKEISNMYLASKILVLTSRYEGFPMVMVEAQCFGLPAVSF
                     DCKTGPSEIIINESTGYIIPYHDDSLFIEKLQRLIDHPKELETFSYNSIKNAQRFTYE
                     NTKYKWLEILEKI"
     gene            7779..8531
                     /gene="wffJ"
     CDS             7779..8531
                     /gene="wffJ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WffJ"
                     /protein_id="ACD37155.1"
                     /translation="MNENEHEIVSIIMPAYNAEATIKSSVYSVIGQTFSKFKLYIIND
                     ASTDCTEEIIRSFEDSRIVYIKNDYNIGVAESRNKGIKKCKGKYIAFIDSDDLWEPNK
                     LELQYIHLNAGMDIVCSNYVTFSENPMRIKNKRIAPQYIRYNDMLKSNFIGNLTGIYN
                     SDKLGKIYQKKIGHEDYVMWLELIKRAKCCYCVQEYLAKYRESNKSLSGNKLRAIVWQ
                     WSIYRKELNMGLISTIYYFSSYIYNGILKRNN"
     gene            8545..9543
                     /gene="gne"
     CDS             8545..9543
                     /gene="gne"
                     /note="GlcNAc-4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gne"
                     /protein_id="ACD37156.1"
                     /translation="MAILVTGGTGYIGSHTVTELLKEGYEVIVIDNLINSAYDVVDRI
                     KKLSKRDFVFYKGDITDESLLFKIFTENNITDVIHFAGLKSVGESFSKPVEYYNINVT
                     GTLKLVNAMLLAGVQHIIFSSSATVYGIPEKIPLTEQCSVGATTNPHGTSKFMAERIL
                     QDIANANKKFHVTLLRYFNPVGAHPSGLIGEQPQGIPNNLVPFLTMVASGKLDMLSVF
                     GNDYPTKDGSGVRDYIHVMDLAEGHIAALKHNPTHSNFHVYNLGTGQGYSVLELIKIF
                     EEVTGIAVKYNIADRRPGDIAECWADPSLAEEELRWRAHRTIEQMMVDAWNWQLKN"
ORIGIN      
        1 attgtggctg cagggatcaa agaaattctc ctggtaactc acgcgtccaa gaacgcagtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgaacagcg cgtgaagcgt
      121 caactgctgg cggaagtaca atctatctgt ccgccgggcg tgaccattat gaacgtgcgt
      181 cagggtgaac ctttaggttt aggccactcc attttatgtg cgcgacctgc cattggtgac
      241 aatccatttg tcgtggtgct gccagacgtt gtgatcgacg acgccagcgc cgacccgcta
      301 cgctacaacc ttgctgccat gattgcgcgc ttcaatgaaa cgggccgtag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactccgtca ttcagacaaa agaaccactg
      421 gatcgtgaag gtaaagtcag ccgcattgtt gaattcatcg aaaaaccgga tcagccacaa
      481 acgctggact cagacatcat ggccgttggt cgctatgtgc tttctgccga tatttggccg
      541 gaacttgaac gcactcaacc tggtgcatgg gggcgtattc agctgactga tgccattgcc
      601 gaactggcga aaaaacagtc cgttgatgca atgctgatga ctggtgaaag ctacgactgc
      661 ggtaaaaaaa tgggctatat gcaggcgttt gtgaagtatg gcttacgcaa cctgaaagaa
      721 ggggcgaagt tccgtaaagg gattgagaag ctgttaagcg aataatgaaa atctgaccgg
      781 atgtaacggt tgataagaaa attataacgg cagtgaggat tcgtggcgaa agtaatttgt
      841 tgcgaatatt cctgccgttg ttttatataa acaatcagaa taacaacgag ttagcaatag
      901 gattttagtc aaagttttcc aggattttcc ttgtttccag agcggattgg taagacaatt
      961 agtatatagg tggttttaat ttccgttgat tatggaatgt tcattacatt aaaaatgtta
     1021 acgcagtgca ctggtagctg ttaagccagg ggcggtagcg ttaaatttga tgctaagcgg
     1081 taaaaagaat atagaatgta atattttatt atttataatc tttaatctgg tgtttcatca
     1141 ttgtttagta tgctcataaa atggtggcaa aatcattttt tttaaattta tagtaaaata
     1201 cgaattcaga cataatgcaa ttatctcaat tgagagagat actaatgtta atatttattg
     1261 taataaatat tggaactata tagataagat aataacttgt ggataagtag cataaattgc
     1321 tgtgaaaatg gctgagcgtg acctgttaac atagaatggt catttattaa tgcttgatat
     1381 attaagaata caagaaaaat atttcatgaa atttacgtta aaaaaaacaa atattcaaaa
     1441 tagaaatgtt gttatttact taataagtga tctacttgca aaggccttgc catttttatt
     1501 tattccagta ttgacaaagt atctcacgcc agagcaatat ggcaatattg cattattcaa
     1561 tataggtgtg gaaacctttc ttataataat tattatggga gggaactcct attataagat
     1621 tgaatatcca aaatttaaag acccaattct ttctttgtat ataattatat ataatgttac
     1681 tttgttattt ttggtatttt ttattttatc gatagtaata tggttttttt atgaaaggat
     1741 ttatattata gatgtgctgc ctttgatatt attatgcgca tattttcagt cgttatttta
     1801 tttatatata agctattatc aatgtaatga aaaagccttg gttgtaggaa tggtgaattt
     1861 atttttctca ctatcaagtt cattgttgat gattctattt cttgtcgaat ttaaacaggc
     1921 tgaaagttca cgatattggt ctttgttagg agggctaatt gcatccgtat tattgttagg
     1981 tagcatttta gctaacaaaa gcaatagaaa atataaatta gtcaataaga ttgattttga
     2041 gttaattaag tttggagtcg gagtgtttcc gcatgctgta agctggtggg ccagaagtgg
     2101 aatggagcgt ctgttgattg gttggttctt atccgtatca acattgggta tttattcgct
     2161 agctatgcag ttgacatcca taatgccatt attttgtaat gcaatcaacc aagcattgat
     2221 gccaagaatt gtaaggtgtt taaatgataa taaaattgaa gaaacaaaga agatattagt
     2281 taagtcatca ctaattgtag caatagcatg tgtattttca tcagtagtta tgcctctgtt
     2341 ccttggatat gttcttaacg atagatacca tgaggcccta gagtatttgc cttatatgat
     2401 tctatcattt gtatttcaag gtgtaattat catttatact aacgttttat atttttataa
     2461 acaagtaaaa tatctttctg ttgctacttt tggaacgtca tgcgttcatt tgttgctatc
     2521 tatgttgttg ataaaaatta atttaaacgt atattctgta ataatatcat ctgtaatcac
     2581 gtttttattc gcatctattg tacttgttag aaaagcatat cagataatga gtgagaggta
     2641 ttaagtgatc ttaactaaga taaaaaatag actaataata tttgtatcta aattattgta
     2701 tcaatcccgt gcctattact atagatgtaa aaacaggaaa aagaaaaaga tatttattta
     2761 tacggattcg agaggttatg aggtaagcaa cttatggaat aagaaaaatc ccttttcatc
     2821 atacgtaggg gatttggtaa aagaatataa tgttgaatat catatatgtg agtactcgag
     2881 tacaacaata attgattttt tatatgaata ttatggggag ggtactagag ggaaaaaata
     2941 tgattacgta attgcgcatg taggattagt tgatttttct ccaaggccaa gaagtatggc
     3001 taacgatata attaagagta agtctcataa aattaaattt tttaaatggg atttacggat
     3061 gtttgataaa tatttggaaa gagaaatttc atcagaaatt tataatggcg aattattatc
     3121 taatttgtat gattgtgagt tcttaaagaa tgaaatcatt ccattattac aaaatatacc
     3181 caacttaatc tacattgggt gtaaccctat tatatccaat tggcgaggta attattggca
     3241 agaccgacca aaagatatca acagaatact tatggcatac aatagcgtta tgttaactga
     3301 attaaaagaa aagaaagttg ttgatattaa agattggaat gaatgcgata taagaaatta
     3361 tacggttgat aatatacatt taaatcgaga aggttataat aaaataacat tgaatctaaa
     3421 acaaatatta acggaaatta agtgatgaag tttatattta caagattagt aatattttta
     3481 ctttctgtat tcgttccaac caaaaaaggt ctttttattt ttggtagttg gtttggtaaa
     3541 aaatatggtg acaatacacg agctttgttt gaacatttat caaatataca accagaaaat
     3601 gtatattggt atacagataa tgaaaagatt gcctctaaaa taatagcctc tgggaataaa
     3661 tgtatttctg gagttaatat gaaaaatatt tttttgcatt tgcgcgcaga agctgtgttt
     3721 tgtaattgtt ctgcaaattc tgatttatta ggggggtata taaataagcg aacaaaggta
     3781 tttaatcttt ggcatggcac acctatgaaa aaaataggca aagatgcagt aaaatccgga
     3841 ttgagtcata ctcggctagg gatgtcgaaa ccaaacttaa cattgagtct aataaaaaaa
     3901 atattaccaa gcacatttta taatttttta aagttagaca cttattattt ggctagttct
     3961 cctgaggttt ctaaaatatt acagggagca atgggggtga acgaagaaaa aataattatt
     4021 tgtggttacc ctaaattaga taaaatcttt atagaaagtg gatatgctga accttacaaa
     4081 attttgtatg cccctaccta tagaggtgaa tataatagtg agttggatat attgacaatg
     4141 ttcggtttta atattgaact tgcggataaa gttttaaaag agaataaagc tactttgact
     4201 attagacttc accctgctaa taaattgcca gtagctgtaa ttaatagaat aaacaataca
     4261 aatacaatat caatagataa tgaagatgat atttatgaaa gtttaggtaa atattcactg
     4321 gtaataactg attattcaag tgtttatttc gatgcattag cagtgggaat taatgtactg
     4381 attgctccat ttggttataa ggattattta gaaaatgacc gagatttgta ttttagccta
     4441 agtgagttat atccagaacg aatgcctatt gattggaagg atttttttga acattttagc
     4501 tattttgcgt cgaataaaag agagcaattt tcatccatca gaaacaagtt ttacagcttt
     4561 ccatatgcac cttgttcgga taagttatta agtttggtat atttaaaatt aggtagagaa
     4621 tatgttaaca aaaagaacta ctaaaaatac tagctttctt tatttctctc ttttttgcat
     4681 ttattttagt gtaagtttta gcccggtcaa tccaacttat atattaggat gtggattaat
     4741 tcttatactt tttatgcaag ttttgcttcg tatgaggata caaaaattta cactgataac
     4801 aattgttttc gctatgatgt ttttattgga acaattgata gggattaaca ttgtctcatt
     4861 ttatcctgag ggtcctgtat atttaacccc tttacttttt tcctattcta ttctgatttc
     4921 tgcttgcgtt attgaaggtt ttcaggggtt aaaaagtgat tctagattga taatatataa
     4981 aagagtttat aattacgcaa tcatattttt agttatagaa ttattcacta gagttttgaa
     5041 ttataacaag gaaaacaatg ggttttattc tttaaaatat agtgttcctt attttgattc
     5101 taattttact gggttggtta ttctttcgtt atgttcatat ttgctttatc taaaatatgt
     5161 taaaggtaat gagcgaaaaa tccaccgtag aattctatat tttttgttat tagcaacgtt
     5221 ttctcgtggt gcaatagctg cattgatctt aactcgtatt ggcttgggta gtaaaaaaag
     5281 aattagaggt aaagcatttt taataatagc agttgctgtt gtcattttta gtatgctaac
     5341 gatagaatat attgagcagt cacaaagtta ttcatcgatt gatgggagtt ttaatagtaa
     5401 gttttacatt attagccaag cgctatcatt ttatgaaagt cttccaattc ttgtgcattg
     5461 gtttggcatt ggaatgggaa atacttctca atttataggt atatttgcac acaatatatt
     5521 tgtaacaatg atcttagaaa tggggatagt tggtactgtt ctttttttat tatatgtatt
     5581 ttatacgtta aagttatcaa atggttatgc attatttata tgggttccta tatttgttgg
     5641 cggaatttca ctttttagtg ctttttcacc atttgctttc atcatctgtg ctttaatttg
     5701 ttgtgaaaat atggtggata aaaaatatga gttttagaga taatccgaaa gtcagtgtct
     5761 taatccctgc gtacaatgta gccgaatgga ttgaagaatc gctattaagt atattagggc
     5821 aatcgtatga aaatatagaa gttattgtag tggatgattg ctcaactgat ggaacgtatg
     5881 aaattatacg tcgggttgcc gagaatgaac cgcgaatgaa ggttttaaga aatgaacgta
     5941 atatgaaaat agtagataca ttaaattatg gcttaaccca ttgcacaggt gattttgttt
     6001 tgaggcatga cggtgatgat gtatcggaaa ttaaacggat tgaggtgcaa cttaaacacc
     6061 tgttagaaaa tgagctagac ttagttggaa ctcaaatgct accaattggt aggtctggtc
     6121 gagttattgg tgctcaaagt agattacctc tatctcatga gttgatcgaa aagattgcaa
     6181 actttagctc accattgacc cacatatgga tatgtaaaaa agagatatat gataaactgg
     6241 gtggctatcg taaggtacca tatgcagaag attttgattt tctgttaagg gcgattgatt
     6301 caggttttaa atgtggcaac acatctgttg cgttaacaaa aattagacat cgtcatggaa
     6361 atacgagtga tattgctagt ctttcccagc gtaaaggata tttttatgca ttagaattgc
     6421 ataaacaaag gaaagcgcaa aactatgata gttataatga tgttgacgca gcaaaattaa
     6481 taaaacacaa taaactagta aactatttcc actcattatc tacaaagtgt ttgcataaag
     6541 catttcagag caataataaa ttagaaatgt ttttttatac attgctatcg gcagtaatgt
     6601 cattttataa tgcgcattat atatatcaaa gaatcaaagt taaaatgcta ttaagcaacc
     6661 agtagtattt ttatattgga aggacttgct aagattattt attttctaca atgagaggaa
     6721 ttgcaaatat aaattatgag gatactaatt ttaatagaag atataacgtt aggtggaggc
     6781 actgaacgcg tagccctcac attagcgaca aatttaaatg ctgacggaat taattgtgat
     6841 atattttcac tttcaaaatc aaatacggat acattttatc cgtcagagaa tattaatata
     6901 tgttatgcta agtcgaaggt tggtgttttt gcaaaaattg aagcaattaa atatgcaaaa
     6961 aaaaacaata tgaaactgtt agttttttct atggggaggt tgtctgttga aatcgcttta
     7021 cttagcagac tcttcttatt taggcatttt ggctgctacg agcatatttc tttctcatca
     7081 ttttctggtt ttatccaaaa aatcaaatta ttcgcatacc gtttatccca tggtgttatt
     7141 tttttgactg agcatgatcg tgatataatt cgacataagt tgtctcatgt cccagttacc
     7201 tctattgaaa acattagtcc ttttcgagca ataaaaaaaa gtaatgtagg tgctaggaag
     7261 aatattgttt tagctgtggg acgattgact aatcagaaaa atttttctcg tttaattaat
     7321 ttatggagca tggtaaacag acctgactgg agtttgatta ttgctggaga tggtccagac
     7381 agagatttcc ttgataaaca aattaatcat ttatgtttga aaaacgtaga gattgttggc
     7441 gctgtaaaag aaattagtaa tatgtatttg gcttctaaaa tactagtgtt gacatctcgc
     7501 tatgaaggtt ttcctatggt aatggttgaa gcccagtgtt ttggactacc tgcagtttca
     7561 tttgattgta aaacggggcc ttcagaaatc ataatcaatg aatctacggg atatataata
     7621 ccctaccatg atgactcgct tttcattgaa aagttgcaaa ggttgataga tcatccgaaa
     7681 gaattagaaa cgtttagcta taattcaatt aagaatgcac aacgttttac atatgaaaac
     7741 actaagtaca agtggttgga aatactagag aaaatataat gaatgaaaat gaacatgaaa
     7801 ttgtatcaat tattatgcct gcttataatg ctgaagcaac gattaaaagt agtgtttatt
     7861 cagttattgg acagaccttt tcaaaattta agttgtatat tattaatgat gcgtcaacgg
     7921 attgtacaga agaaattata agaagttttg aagattcaag gattgtttat atcaaaaatg
     7981 attataatat aggtgtggcc gaatcaagaa ataaaggaat taaaaaatgt aagggcaaat
     8041 atattgcttt cattgacagc gatgacctgt gggaacctaa caaattggaa ttacaatata
     8101 ttcatttgaa cgcaggtatg gacattgttt gttcaaatta tgtaactttc tctgaaaacc
     8161 caatgcgtat taaaaataaa agaatagcac cacagtacat aagatataat gatatgctga
     8221 agtctaactt tatcggtaat cttacaggga tctataactc ggataagtta gggaagatat
     8281 atcaaaaaaa aataggccac gaagattatg ttatgtggct tgaattaata aagcgtgcaa
     8341 aatgttgtta ttgcgttcaa gaatacctgg ctaaatatcg cgagtcaaat aaatcccttt
     8401 cgggaaataa gttaagagca attgtatggc aatggagtat ttatagaaaa gaattaaata
     8461 tgggattaat cagtacaata tattactttt caagttatat ttataatggg atcttaaaaa
     8521 gaaacaacta aactggggag gggtatggct attttagtta caggcggaac aggttatatt
     8581 ggatctcata cggttacaga gttacttaag gagggatatg aagttattgt aattgataat
     8641 cttattaact ctgcatatga tgtggtcgat aggataaaga aactttcaaa acgtgatttc
     8701 gttttttata aaggtgatat tactgatgaa agtttattgt ttaaaatatt tactgaaaat
     8761 aacatcactg atgtgattca ttttgctggt ttaaaatctg taggcgaatc tttttctaaa
     8821 ccagttgaat attacaatat aaatgttaca ggcacgctaa aattagttaa tgcaatgctt
     8881 cttgctggtg tgcaacatat tatttttagt tcctcagcaa ctgtttatgg aatacctgaa
     8941 aaaataccat taacagaaca atgttctgta ggtgcaacaa ccaacccaca tggaacttca
     9001 aagtttatgg ctgagcgcat actacaagat attgctaatg caaataaaaa attccatgtc
     9061 accttattaa gatatttcaa cccagtgggt gctcatccat caggattaat tggtgaacag
     9121 cctcaaggaa ttccaaataa tttggttccg tttttaacta tggttgcaag tggtaaactt
     9181 gatatgttat ctgtttttgg caatgattac cccacaaaag atggaagcgg agttcgtgat
     9241 tatatccacg taatggattt agctgaaggc catatagcag cattaaaaca taatccgacg
     9301 cattctaatt tccatgtata caatttaggt actggtcaag gatactctgt attagagttg
     9361 attaagatat ttgaagaagt tacgggaata gcagtgaaat ataatatagc tgatagaagg
     9421 cccggtgata tagcagaatg ttgggctgat ccttctttag cagaagagga gctaagatgg
     9481 agggctcata ggactatcga gcagatgatg gtagatgcat ggaattggca acttaaaaac
     9541 tgatatagct aagtagtaaa tttattttga aaggctatga agcttcattt gaactaagtt
     9601 aattttattt tatccatagt gcacacctta ctgtgcgtat gataggagta aacaatgtca
     9661 aagcaacaga tcggcgtcgt cggtatggca gtgatggggc gcaaccttgc gctcaacatc
     9721 gaaagccgtg gttataccgt ctctattttc aaccgttccc gtgagaaaac ggaagaagtg
     9781 attgccgaaa atccaggcaa aaaactggtt ccttactata cggtgaaaga gttcgttgaa
     9841 tctctggaaa cgcctcgtcg catcctgctg atggtgaaag caggtgcagg cacggatgct
     9901 gctattgatt ccctcaagcc atacctcgat aaaggtgaca tcattattga tggtggtaat
     9961 accttcttcc aggacactat tcgtcgtaat cgtgagcttt ctgcagaagg ctttaacttc
    10021 atcggtacgg gtgtttctgg tggtgaagaa ggtgcgctga aaggaccttc catcatgcct
    10081 ggtggccaga aagaagccta tgaactggtt gctccgatcc tgacgaaaat cgccgcagtg
    10141 gctgaagacg gtgagccatg cgttacctat atgggtgccg atgggcgcag gtcactatgt
    10201 gaagatgggt tcacaacggg tatcgaatac ggtgatatgc aactgattgc tgaagcctat
    10261 tctctactta aaggcggcct gaacctttcc aacgaagagt tggcgcagac ctttaccgag
    10321 tggaataacg gtgaactgag cagctacctg atcgacatca ccaaagatat cttcaccaaa
    10381 aaagatgaag acggtaacta cctggttgat gtgattctgg atgaagcagc aaacaaaggt
    10441 acgggcaaat ggaccagcca gagtgcgctg gatctcggcg aaccgctgtc gctgattacc
    10501 gagtctgtgt ttgcacgtta tatctcttct ctgaaagatc agcgtgttgc cgcatctaaa
    10561 gttctctctg gcccgcaagc gcagccagca ggcgataagg ctgagtttat cgagaaagtt
    10621 cgtcgtgcgc tgtatctggg caaaatcgtt tcttatgctc agggcttctc tcagctgcgt
    10681 gctgcgtctg aagagtacaa ctgggatctg aactacggcg aaatcgcgaa gattttccgt
    10741 gctggctgta tcatccgtgc gcagttcctg cagaaaatca ctgatgcata tgctgaaaac
    10801 cccctgctcg ctaacttgct gctggctccg tacttcaagc aaattgccga tgactatcag
    10861 caggcactgc gcgatgttgt cgcttatgca gtacagaatg gtatcccggt tccgaccttc
    10921 gccgctgcgg ttgcctatta tgacagctac cgcgccgctg ttctgcctgc gaacctaatt
    10981 caggcccagc
//

</pre>