<pre>LOCUS       EU296424               13480 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Escherichia coli serogroup O129 O-antigen gene cluster, complete
            sequence.
ACCESSION   EU296424
VERSION     EU296424.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13480)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 13480)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..13480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O129"
     misc_feature    1139..8869
                     /note="O-antigen gene cluster"
     gene            1139..2224
                     /gene="rmlB"
     CDS             1139..2224
                     /gene="rmlB"
                     /note="dTDP-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ACD37174.1"
                     /translation="MRILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            2224..3123
                     /gene="rmlD"
     CDS             2224..3123
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ACD37175.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPKFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPYNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            3182..4060
                     /gene="rmlA"
     CDS             3182..4060
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ACD37176.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            4065..4610
                     /gene="rmlC"
     CDS             4065..4610
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ACD37177.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYLI"
     gene            4626..5882
                     /gene="wzx"
     CDS             4626..5882
                     /gene="wzx"
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ACD37178.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQISSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            5912..6802
                     /gene="rfbF"
     CDS             5912..6802
                     /gene="rfbF"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RfbF"
                     /protein_id="ACD37179.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNSTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREIVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            6795..7700
                     /gene="rfbG"
     CDS             6795..7700
                     /gene="rfbG"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RfbG"
                     /protein_id="ACD37180.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQVSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            7721..8869
                     /gene="wzy"
     CDS             7721..8869
                     /gene="wzy"
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ACD37181.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKLFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSSYFFGAGAIISFVNKKYLKWLLIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIGIVPLEILQKTGVIGVYGSLVPMLLIFVKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
ORIGIN      
        1 attgtggctg cagggatcaa cagaaatcct cctggtaact catgcgtcca agaacgcggt
       61 cgaaaaccac ttcgacacct cttatgaatt agaatctctc cttgaacagc gcgtaaagcg
      121 tcaactactg gcggaagtac agtccatttg cccgccgggc gtgaccatta tgaacgtgcg
      181 acagggcgaa cctttaggtt tgggccactc cattttatgt gcacggcctg ccattggtga
      241 caatccattt gtcgtggtgc tgccagacgt tgtgatcgac gacgccagcg ccgacccgct
      301 gcgctacaac cttgctgcca tgattgcgcg cttcaacgaa acgggccgca gccaggtgct
      361 ggcaaaacgt atgccgggtg acctctctga atactctgtc atccagacca aagagccgct
      421 ggaccgcgaa ggtaaagtca gccgcattgt tgaattcatc gaaaaaccgg atcagccgca
      481 gacgctggac tcagacatca tggccgttgg tcgctatgtg ctttctgccg atatttggcc
      541 ggaacttgaa cgcactcagc caggtgcatg ggggcgtatt cagctgactg atgccatcgc
      601 tgaactgggg aaaaaacagt ccgttgatgc catgctgatg acaggtgaca gctacgactg
      661 cggtaaaaaa atgggctata tgcaagcgtt cgtgaagtat ggactacgca acctgaaaga
      721 aggggcgaag ttccgtaaag ggattgagaa gttgttaagc gaataatgaa aatctgaccg
      781 gatgtaacgg ttgataagaa aattataacg gcagtgaaga ttcgtggcat aagttttgct
      841 cattgaattt tcctgccgtt gccgttttta taaagcctat aaaaacaaga agttacatag
      901 ctcatattat tcaaattttt ccaggatttt ccttgtttct acggctgttt ggtaagacaa
      961 ttagcgtttg aatttttcgg atttggcgcg agtggctaac gctcgccaca tcgttgagat
     1021 gcatgcagtg cactggtagc tgttaagcca agggcggtag cgtgtattaa tacctctatt
     1081 aatcaaatct ggagcagtct atttcacagc atgctctata gctatatgga ataaaaaagt
     1141 gaggatactt gttactggtg gcgcagggtt tattggttct gctgttgttc gtcacataat
     1201 aaataatacg caagatagtg ttgttaatgt cgataaatta acatacgccg ggaacctgga
     1261 atcactcgct gatgtttctg attctgaacg ttatgctttt gagcatgcag atatctgcga
     1321 tgctgtagcc atgtcgcgta ttttcgcaca gcaccagcca gacgcggtga tgcacctggc
     1381 ggcagagagc cacgttgacc gttcaataac gggccctgcg gcatttattg aaaccaatat
     1441 tgtggggact tatgttcttt tagaagcggc gcgcaattac tggtctgctc tgaatgatga
     1501 aaagaaaaaa agtttccgct ttcatcatat ttctactgat gaagtttatg gtgacttacc
     1561 ccatccggat gaagcaaata ataacgaagc gttaccgcta tttacggaaa cgacagctta
     1621 cgcgccaagt agcccgtatt ctgcgtctaa agcttccagc gatcatttgg tacgtgcctg
     1681 gaagcggact tatggtttac caaccattgt gactaattgc tcgaacaatt atggtcctta
     1741 tcatttcccg gaaaaactta ttccattggt tattctgaat gctctggaag gtaaggcatt
     1801 acctatttat ggtaaagggg atcaaattcg cgactggttg tatgtagagg atcatgctcg
     1861 tgcgttatat accgtcgtaa ccgaaggtaa agcgggtgaa acttataaca ttggtggaca
     1921 taacgaaaag aaaaacatcg acgtagtgct cactatttgt gatttgttgg atgagattgt
     1981 accgaaagag aaatcttatc gtgagcaaat tacttatgtt gccgatcgcc cggggcatga
     2041 ccgccgttac gccattgatg ccgataaaat tagccgcgaa ttgggctgga aaccgcaaga
     2101 aacgtttgag agcgggattc gtaaaacggt ggaatggtat ctggctaata caaattgggt
     2161 tgagaatgtg aaaagcggtg cttatcagtc gtggattgaa caaaactatg agggccgtca
     2221 gtaatgaata tcctgctttt cggcaaaaca gggcaggtgg gttgggaact gcagcgtgct
     2281 ctggctcctc tgggtaactt gattgcgctc gatgttcact ccactgatta ttgtggagat
     2341 ttcagcaacc ccgaaggtgt ggctgaaacc gtcaaaaaaa ttcgccccga tgttattgtt
     2401 aatgctgctg ctcacactgc agtagacaaa gcggaatcag aaccgaaatt cgcgcaatta
     2461 cttaacgcga caagcgtcga agcgattgca aaagcagcta atgaagtcgg agcctgggtt
     2521 atacactact ccactgatta tgttttccca ggcaacggtg acacgccatg gctggaaacg
     2581 gatgcaacag caccgctaaa tgtctacggt gaaaccaagc tagctgggga aaaagcgtta
     2641 caagaacatt gcgcaaagca tcttattttc cgtaccagct gggtatacgc tggtaaagga
     2701 aataactttg ccaaaacgat gttgcgcctg gcaaaagaac gcgaagagtt ggctgtaata
     2761 aacgatcaat tcggcgcacc aacgggggct gaattactgg ctgattgcac cgctcatgcc
     2821 attcgcgtgg ccgcaaataa accagaagtt gctggtttgt accatctggt tgctggtggt
     2881 acaacaacct ggcacgatta tgccgctctg gtatttgaag aagcgcgcag agcggggatc
     2941 aaccttgctc ttaacaaact taatgccgtg ccgacaacgg cctatcccac accagcacgt
     3001 cgtccctata attctcgcct caataccgaa aaatttcagc agaactttgc gcttgtcttg
     3061 cctgactggc aggtgggcgt gaaacgtatg ctcaacgaat tatttacgac cacggcaatt
     3121 taacaaatta ttgcatctcg ctcatgatgc cagagcgaga tgaattaaaa ggaatggtga
     3181 aatgaaaacg cgtaaaggta ttattctggc tggtggttcc ggcactcgtc tttatcctgt
     3241 gactatggct gtgagcaaac aactgctacc gatttatgat aaaccaatga tttattatcc
     3301 gctttcaacg cttatgttgg cgggtattcg agatattctg attatcagta cgccgcagga
     3361 tacaccacgt tttcaacaat tgcttggtga tggaagccag tgggggctta atctgcaata
     3421 taaagtacaa ccgagtccgg atggtctggc gcaagcattt atcattggtg aagaatttat
     3481 aggtggtgat gattgtgcgc tggtacttgg tgataatatc ttctatggtc acgacctgcc
     3541 gaaattaatg gataccgctg ttaacagaga gagtggcgca acggtttttg cctatcacgt
     3601 aaatgatcca gagcgctacg gtgtggtgga atttgatgat aacggtacgg ctattagtct
     3661 ggaagaaaaa ccgctggaac caaaaagtaa ctatgcggtt actgggcttt atttctatga
     3721 caatgacgtt gtagaaatgg cgaaaaacct taaaccatct gcacgtggcg aactggaaat
     3781 tactgatatt aaccgtattt atatggaaca aggacgtttg tctgtcgcta tgatggggcg
     3841 tggttatgca tggctggaca cggggacaca tcaaagtctt attgaagcaa gcaacttcat
     3901 tgcaacaatt gaagagcgtc aggggctgaa agtttcctgc ccggaagaaa ttgcttaccg
     3961 taaaggattt attgatgctg agcaagtgaa ggtattagcc gagccgctga agaaaaatgc
     4021 ttatggtcag tatctgctga aaatgattaa aggttattaa taaaatgaac gtaattaaaa
     4081 ctgaaattcc tgatgtgcta atttttgaac caaaagtatt tggtgatgag cggggctttt
     4141 ttatggaaag cttcaatcag aaagttttcg aagaggctgt aggacgaaag gttgaatttg
     4201 ttcaggataa ccattctaaa tcaactaagg gtgtgttacg cggactgcac tatcagctgg
     4261 aaccttatgc tcaaggaaaa ctggtacgct gtgttgtcgg tgaagttttt gacgtagctg
     4321 ttgatattcg gaaatcatca ccaacatttg gcaagtgggt tggtgtaaat ttgtcggcgg
     4381 agaataagcg tcaattatgg ataccggaag gattcgctca tgggttttgc gttcttagtg
     4441 atgaagcaga atttgtctac aaaacaaata atttctattc aaaaatgcaa gagcgtggga
     4501 ttttatggag cgacaaaagt ataaatatag aatggccggt tcaaaatcca ttgctttctg
     4561 ataaagatat taatggtcaa aaatttgtag atgctgatta tttgatatga taaagaaatg
     4621 taataatgag cataataaaa aatagtgtct ggaacctttt tggctatgca ataccaactt
     4681 taattgctat cccctcgcta ggatttctcg ctcgaggttt ggggcctgaa ggtttcggtg
     4741 tttatacaat tgcaattgca cttgttgggt atgctggaat ttttgatgta ggcctgactc
     4801 gctctgttat tagagaaatt gcaattcatc gtgataatca tcatgaaaga accaaggtaa
     4861 tttcaacaag tacatctttt ttggtgctat tttcatgctt tggtgctttt ttattattga
     4921 ttttctctga tggaattgtt aattatctaa aaatttctgg tgttgaacat agtgatatac
     4981 aactagcatt taaactgttg gctatttgca ttccattatt tattctaaat caattatggt
     5041 cagccattct tgagggggat gaaaaatttg gcattgtaaa tattcaaaaa tctatatcaa
     5101 gctcttgcat cgcgggaatt ccggccatat ttgtttttta tagtgctaca ttgtcggcag
     5161 cggttgctgg tttaatattt gcaagagtta tttcgatttt agtctctgcc tattatgtca
     5221 ggaatgatat taaaatttcg ggggttcatt tttgttataa aacctttaaa cgactctttt
     5281 tctttggcgg ttggatgaca gtaagtaata tcataagtcc ggtcatggtg tattttgatc
     5341 gatttatagt gtcaaatatt atgggggcag ataaagttgc attttattct gcaccagcgg
     5401 aggttattct aaaattagga ataatacctg cagcaatcgg gagagcagtg tttccaaggt
     5461 taagtaacat caaagacttt aaagaattta aacgtaatgt aaataaatca ttgcttttaa
     5521 tgtttctaat ctgtttgccg gtgataatca taggcttgtt atattcaggg cttgtattga
     5581 aaatatggtt tggtgagaat taccaaatta gttcctttaa tatattaaat gtgttattga
     5641 tcggtttttt cttcaatgcg ctggcaatga taccattctc tgcaatccag gcattaggaa
     5701 aatctaaaat tactgctttg attcattgtg ctgaattggt tccttattta gcccttttgt
     5761 attttatggt cgaaaaatat gggttactgg gtgccgcaat atcctggagc atacgtgtaa
     5821 ttttagatgc tctgttatta caatggcttt atactagaat gtgttctgta tatgaaaact
     5881 aaaattaacc aattactgag tcacttggat aatgaatagt aatatttacg ctgtcattgt
     5941 gacatataat cccgaactta aaaatctgaa tgcactgatc actgaactaa aggagcaaaa
     6001 ttgttacgtt gtagtagtcg ataatagtac taatttcacc ttgaaagata aattagcaga
     6061 cattgaaaaa gttcatttaa tttgtcttgg taggaatgaa ggtatagcaa aagcacaaaa
     6121 tattgggatt cgatattctc ttgaaaaagg ggcagagaag ataatttttt ttgatcaaga
     6181 tagccgtatt cgcaatgagt ttatcaaaaa actttcctgc tatatggata atgaaaatgc
     6241 aaaaatagct ggcccagttt ttattgatag agataagtca cattattatc ctatttgtaa
     6301 tatcaaaaaa aatggtcttc gagagaaaat tcatgtcact gaaggacaga caccgtttaa
     6361 aagttcagta acaatctcat ccggaaccat ggtttcaaaa gaagtttttg agattgttgg
     6421 aatgatggat gaggaacttt ttattgatta tgtcgataca gaatggtgtc ttagatgctt
     6481 aaactatggc atattagttc atatcattcc tgatatagaa atggttcatg ctattgggga
     6541 taagtcagta aaaatctgtg ggattaacat accaattcac tcgccagtac gtcgttatta
     6601 tcgagtaaga aatgcatttc ttttgcttag aaaaaatcat gtgcctcttt tactctctat
     6661 tagggaaatt gttttttctt taattcatac gactttgatt atcgcaactc aaaaaaataa
     6721 aattgaatat atgaaaaaac atattttggc tacgctggat ggaataagag gaattactgg
     6781 gggcggtcgt tataatgcat agttctgatc aaaaaagagt agctgtactt atggctacgt
     6841 ataatggcga atgctggatt gaggaacaat taaaaagtat tattgaacaa aaagatgtag
     6901 acatctcaat ttttatatca gatgatttat caactgataa tacattaaat atttgtgaag
     6961 aatttcaagt atcatatccc tcaattataa atatacttcc aagcgtcaat aagtttgggg
     7021 gggcaggtaa aaatttttat agattaatta aagatgtcga tcttgaaaac tatgattata
     7081 tctgttttag tgatcaggac gatatttggt ataaagataa aataaaaaat gcaattgact
     7141 gcttggtatt taataatgcg aattgttatt ccagcaatgt aattgcatat tatccatcag
     7201 ggcaaaaaaa tttagttgat aaggctcagt cgcaaactca attcgattat ttttttgaag
     7261 cagcaggtcc ggggtgtaca tatgttataa aaaaagaaac gcttattgaa tttaagaagt
     7321 tcatcataaa taataaaaat gctgcacaag atatctgtct gcatgattgg tttttatatt
     7381 catttgcgcg gacgagaaat tattcgtggt atattgatag aaaaccaaca atgctgtaca
     7441 gacagcatga aaacaaccaa gtgggagcaa acattagttt taaagcaaaa tacaaacggc
     7501 ttggtttggt gagaaacaaa tggtatcgaa aagaagtaac taaaatagct aatgccttag
     7561 ctgatgatag ctttgtgaat aatcaattgg gtaaaggtta tataggcaat ttgatcttgg
     7621 ctttaagttt ttggaagtta aggcggaaaa aggctgataa aatttatatt ttattaatgt
     7681 tgattttaaa catattttaa aaaaacatca ccttacgaat atgaataata taaataaaat
     7741 ttttataaca tttttatgta ttgaactgat tattggtggt ggtggaagat tactggagcc
     7801 attgggaata ttccctttgc gatatttatt atttgtattt agttttatac ttttaatttt
     7861 taatttagtt acattcaatt tttcaatcac ccaaaaatgt gtcagtcttt ttatatggtt
     7921 gcttttattc cctttttatg gcttctttgt cggcttatta gctggtaata aaataaatga
     7981 catactgttt gatgtgcaac catacctttt tatgctgtca cttatatatc tatttacact
     8041 aagatatact ttaaaattat ttttatgtga gatttttatt aaaatagtta atgcatttgc
     8101 attatatgga tcactgttat atatttcata cataattttg ttgaatttcg gtttgttaaa
     8161 ttttaattta atttatgaac acttatcatt gactagcgag ttcttttttc gtcccgatgg
     8221 ggcttttttt tcaaaatcct cctacttttt tggtgccggt gcaattatca gttttgtcaa
     8281 caaaaaatat ttaaaatggc tactaatagt gcttgcgata ttattgacag aatcaagagg
     8341 tgtattactt tttacaacat tatcactgtt attagccagt tttaaattac ataagctata
     8401 tttaaatact attataataa tattgggcag cgttctattt ataattatgc tttacatggt
     8461 cggatcacgc agtgaagatt ctgactctgt tagatttaat gatttatatt tttattataa
     8521 aaatgttgat ttagcgacgt tcttgtttgg aagaggattt ggttcattta tattagatcg
     8581 attaaggatt ggaatagtac ctcttgagat acttcagaaa acaggcgtta ttggtgtata
     8641 tggatcatta gttcctatgt tgcttatctt tgtgaaaggc tattttttaa atagtacaaa
     8701 aacatcatta atgatgtcgt taatactttt tttcagtatt accgtttcta taactaatcc
     8761 atttcttttt acacccatgg gaatttttat tataggcgtt gtagttttat gggtattttc
     8821 tatagaaaat atccaaatta gtaataacct cacttctgga gcaaaataaa ggatgttaaa
     8881 aatagggaag ttattgacca gttctttttt tagttatttt ttaattggaa ttgtcaatac
     8941 tgctttacat tggggggtat tttatgcgtg ttataataat ttagcttttg gtcaaggccg
     9001 aagtaacatt gttggtttta tatgtgcagc aaccttttct ttttttgcaa atgcaagata
     9061 ctcatttaaa gtatctgcaa ccaaggctag atattttata tttatatttt ttatgggggc
     9121 gatgagttat ttattcggcg ttttatttga tctcttagct ttatctccaa tatttacatt
     9181 gttcaccttt tctcttttta gtttggtttt aggatattgc gcatcaaaat atttcatttt
     9241 tcgttagtaa acgatttgtt aaaaacacaa ttacttgcac attagctaaa tagtttatat
     9301 gaggggctta tgtatcactc ttttaatatc gctgtaatta ttccttgtta taatgagcag
     9361 aaggcaatag caaaagtcat aaatgatttc aaaacgaata ttccaactgc ttctatatat
     9421 gtatttgata ataattcgac tgacagtaca gcacaagtgg cagaggatgc tggagcaaca
     9481 gttcactttg ttcctctgaa aggcaagggg aatgtcgtaa gacgtatgtt ctcagatgta
     9541 gatgcggata tttatctaat ggttgatgga gatgacactt atgatgcaag ttctgctcca
     9601 gaaatgatta accatctcat aaaaaatcaa ttggatatgg ttgtaggatg tcgtcaagag
     9661 aatggagatc aaaatacata cagaaaaggg catcgctggg gaaataaact gttgactggt
     9721 actgttcaga ctatctttaa agggaatttt actgatatgt tgtcggggta tcgtgtattt
     9781 tcctgacgct atgttaaatc ttttccatgt ctatcaaggg gatttgaaat cgaaactgag
     9841 cttacaattc atgctttaga attacgtatg aagtatggtg aggttaatac aaaatatggc
     9901 gagcgacctg aagggagtgt gagcaaacta tcaacttgga gtgatgggtt caagattctg
     9961 aaaacaataa ttaaacttta ctcgcttgaa cggccactat acttttttag tattatcggc
    10021 gttttacttg ctgctttgtc tataatttta ggattgccca tcattgttga ttatattgat
    10081 acaggtttgg tgagaagatt tccgactgcc tttttaactg cctccatcat gttatcgtca
    10141 ataatggcat ttgtatgtgg cattattttg cattcaaata caacaactag acgtgaaatg
    10201 aaagcattat tttatctttc tgaaaaaaaa ctataaactg ataatgtaaa tagagcattt
    10261 tactatgatt gaattgccgt tgtatttgta tgttgtgtat tgttttgctt ttggaatgat
    10321 attttttgtc attggtaata ttgtattgtc atcttttttt aaggaggggg tggcaggact
    10381 taacaaattt tctttatttt ttcattgcct atgcatagga atgtctttaa gtattttgat
    10441 aatttacttt ctgggcatgg tcgggcaatt taataagatt gctatctttt taatttttac
    10501 ggtgtgttgg gttttaagca ttattaaacg tcagcaattt agatggctgg caataaataa
    10561 tattgagttc agtacgctgt gtgttatttt atttcttgtt ttgatttttg cggttacatt
    10621 gttatcgtcc ctgcgagctc ccggtgattg ggatgatact atgtatcact tacctttggc
    10681 tcgttcatta gttgagcacc atgcaattgt tgttgaacaa tatttgcgtt ttcctctgtt
    10741 cccgcaaaat gcagatcttt taatggcttt aggtttacag ttaggagatg taaggttagc
    10801 tcagttttag caaatatctg tttttttgta atagcctgtg gacttgttgg ctgttcatgg
    10861 gaaattacga aaacttatta tcctggcatt attgctacaa tcctactatt tactatcaat
    10921 ccgcttaaag atcatcttgg ttatgcatat attgatttaa cgctatcttt gttttgtttt
    10981 gctgcagcca gtattcttat atatattcgc tcagaaaaca ataaaacaca aaatgaatta
    11041 atttttttat gtggtttttt tattggaatt gcttgtggcg taaaactatt tggtgccata
    11101 tatggagtca tcatatttgt tatattgata gcgaaaaaga gaaacataca gacatttctt
    11161 atttttagct tggcaacaat tcttggtggc atttattggt atgtccgtag tttttggatc
    11221 tctggtgatc cttttagtcc tgctggagga aatatatttg gtcactacct atggaatgaa
    11281 attgatctac aagttcaaac agcagaacaa gctcgacatg gtataagtcc tgccacatta
    11341 gatttgcaac aagcaattaa taaagttggc agtgatgttg ttttttatgc tatactgtct
    11401 gtctttacac ttaggaataa taaaatactt tgggtatttt tctcgattgt tttaacatat
    11461 gtcttatttt ggttttctgt tacgcaagtt gacaggtatt tatcacccat ttacccattg
    11521 tcagtattgc tggttgcgat ctcaattgca agtttaataa aagatatcaa cattacaaat
    11581 tacaagttgg gacgatattt tatccttgcg tgtattattt tttttcgggg cgatcgttgt
    11641 attttgaatt caaatatttt tattctgaat acttgaattt taattcacgc ttagaaagtc
    11701 gctatagcta tgaactgatg aagaaagcaa gtgaatatag tgagttatat ggagataatc
    11761 ttatccaatt aggcttggag gatggtattt acttctataa agggatggca attggtgacg
    11821 tctttggatt agcaagatat agtgattgga ctatttgtaa tcccgagtgt gaggtaatcc
    11881 cacaggatga tttaatagag aagatgaaat cttttaatag ttcgtttatt gttatatcta
    11941 aacgaagcta tgctaatttt aatccagaaa agtatccaaa attcaaagtt cttatggata
    12001 cacccaatgg catacttatc gctataaagt aagaagcatg ctgtaaccat atgtattcct
    12061 tgattagaag atgaaaatca cgacggttaa taatataacg gttaatagac ggttaataat
    12121 atagaagaca ggagtaaaca atgtcaaagc aacagatcgg cgtagtcggt atggcagtga
    12181 tggggcgcaa ccttgcgctc aacatcgaaa gccgtggtta taccgtctct attttcaacc
    12241 gttcccgtga aaagacggaa gaagtgattg ccgaaaatcc aggcaagaaa ctggttcctt
    12301 actatacggt gaaagagttt gttgaatctc tggaaacgcc tcgtcgcatc ctgttaatgg
    12361 tgaaagcagg tgcaggcacg gatgctgcta ttgattccct taagccatac ctcgataaag
    12421 gtgacatcat cattgatggt ggtaatacct tcttccagga caccattcgt cgtaacggag
    12481 agctttctgc cgaaggcttt aacttcatcg gtaccggtgt ttccggcgga gaagaggggg
    12541 cgctgaaagg gccgtccatc atgcctggtg gccagaaaga agcctatgaa ctggttgcgc
    12601 cgatcctgac caaaatcgcc gccgttgctg aagatggcga accgtgcgtt acctatatcg
    12661 gtgctgatgg cgcaggccat tatgtgaaga tggttcacaa cggtatggaa tacggtgata
    12721 tgcaactgat tgctgaagcc tattctctcc tgaaaggcgg cctgaatctc tctaacgaag
    12781 aactggcaca gacctttacc gagtggaata acggtgaact gagcagctac ctgatcgaca
    12841 tcaccaaaga catcttcact aaaaaagatg aagacggtaa ctacctggtt gatgtgatcc
    12901 tggatgaagc ggctaacaaa ggtaccggta aatggaccag ccagagcgcg ctggatctcg
    12961 gagaaccgct gtcgctgatt accgagtctg tgtttgcacg ttatatctct tctctgaaag
    13021 atcagcgcgt tgccgcatct aaagttctct ctggtccgca agctcagtca gcaggcgaca
    13081 aggctgagtt catcgaaaaa gttcgccgtg cgctgtatct gggcaaaatc gtttcttacg
    13141 ctcagggctt ctctcagctg cgcgcagcgt ctgaagagta caactgggat ctgaactacg
    13201 gcgaaatcgc gaagattttc cgtgctggct gcatcatccg tgcgcagttc ctgcagaaaa
    13261 tcaccgatgc ctatgccgaa aatccgcaga tcgctaacct gctgctggct ccgtacttca
    13321 agcaaattgc cgatgactac cagcaggcgc tgcgcgatgt cgtcgcttac gcagtacaga
    13381 acggtattcc ggttccgacc ttcgccgctg cggttgccta ttatgacagc taccgttctg
    13441 ctgttctgcc tgcgaaccta atccaggcgc agcgcgacta
//

</pre>