<pre>LOCUS       DQ465248               11783 bp    DNA     linear   BCT 13-AUG-2007
DEFINITION  Escherichia coli strain O126 O antigen gene cluster, complete
            sequence.
ACCESSION   DQ465248
VERSION     DQ465248.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11783)
  AUTHORS   Liu,Y., DebRoy,C. and Fratamico,P.
  TITLE     Sequencing and analysis of the Escherichia coli serogroup O117,
            O126, and O146 O-antigen gene clusters and development of PCR
            assays targeting serogroup O117-, O126-, and O146-specific DNA
            sequences
  JOURNAL   Mol. Cell. Probes 21 (4), 295-302 (2007)
   PUBMED   17452091
REFERENCE   2  (bases 1 to 11783)
  AUTHORS   Liu,Y., Debroy,C. and Fratamico,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAR-2006) ERRC-USDA, 600 E. Mermaid Lane, Wyndmoor,
            PA 19038, USA
FEATURES             Location/Qualifiers
     source          1..11783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="O126"
                     /db_xref="taxon:562"
     misc_feature    1..11783
                     /note="O antigen gene cluster, complete sequence"
     gene            43..1086
                     /gene="wzy"
     CDS             43..1086
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O antigen polymerase"
                     /protein_id="ABE98419.1"
                     /translation="MYIKFYGFSLMENITIYDYVIVNNGLFNKDAVIVFFIIFYLFGC
                     LINIVSNYVSCIYVLLVLIPLSLLFALNGPDKASYLSLYNAIDTVSDVFKYYNTIDSP
                     LYYITMVLFKNIGLTFYSFMVVQNLIISFSLSYLIYKYKWGYHIAMWLYFTHLLVPFS
                     VRGHLVIITILFANRSVVSKIISSLGLVAIHPLISALPITYQLRGLIRGKRSILIIVL
                     TICALVFSSSYLISKYTSYAINGDEYVKNAGLSLIYIVDAIELLLFWSIIRPIALIDK
                     LFLLFPVVLSFGTIIIPMLGRFSYACDLVYILILINNANRLKNKKTLFMCLSLIALIR
                     FVKNYYSIEYFIL"
     gene            1083..2084
                     /gene="wbgK"
     CDS             1083..2084
                     /gene="wbgK"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ABE98420.1"
                     /translation="MMLFFKNPKNKKSTGILYLQTLLHERLSNVRVITSTIMIIKRYF
                     NIMKSTEVAIFLPFTFSCIFAKKNDLVIMPDDSIRSITSVSKVRYENKKILKWLFQNI
                     RLIITKNIISLKKDVVYLTVSKDDQWSLQSKFPKHKIVYIPHPIQSASNKEDVLDRCE
                     NISTICFMNLQEHYSVNPSTFIRPKTKVNLNDLHNKVNIIFHGSYSQAWYGKYMYYYG
                     KANCKAVRYVEDFEQFYNNIDLIILPLAAGAGIKNIVLNSVYRNKLILGTEEAFSGIP
                     KEFTRKLIIDSNDDILTKINSEVECLAIKESCNILRDYLVVEHTEKAFMDTIFKNII"
     gene            2094..2987
                     /gene="wbgL"
     CDS             2094..2987
                     /gene="wbgL"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ABE98421.1"
                     /translation="MSIIRLQGGLGNQLFQFSFGYALSKINGTPLYFDISHYAENDDH
                     GGYRLNNLQIPEEYLQYYTPKINNIYKLLVRGSRLYPDIFLFLGFCNEFHAYGYDFEY
                     IAQKWKSKKYIGYWQSEHFFHKHILDLKEFFIPKNVSEQANLLAAKILESQSSLSIHI
                     RRGDYIKNKTATLTHGVCSLEYYKKALNKIRDLAMIRDVFIFSDDIFWCKENIETLLS
                     KKYNIYYSEDLSQEEDLWLMSLANHHIIANSSFSWWGAYLGSSASQIVIYPTPWYDIT
                     PKNTYIPIVNHWINVDKHSSC"
     gene            2994..3764
                     /gene="wbsT"
     CDS             2994..3764
                     /gene="wbsT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ABE98422.1"
                     /translation="MIKEFVVERPYFSFLLCVNKDEGFLNDALESIINQDYSYEYEII
                     VIANNCDDNLWAYLNSVESDKLKIHRTNIGQLAFNLNYGANIARGEYIVRMDADDVST
                     PNRLKILEAAIQENNYPDVIGTSVYFISETGRIIKKYVPPLGEVKSALIKNPFVHPTV
                     AIKKVSLFKIGGYLGGFQSEDNDLWIRMLRAHEFKLVNIDSVTLFYRINDHQTRGRVL
                     PYAEVASYALREFLLKKRIKYFAALLINIGKALILPKR"
     gene            3768..4898
                     /gene="wbsU"
     CDS             3768..4898
                     /gene="wbsU"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ABE98423.1"
                     /translation="MKLMKIMHTVLNTFTAKKFVEPLLDNVNEQHNKEKIIHKILIDP
                     VNGDERFLKELHVQYEIKKFDIDYLFIKHIIELYFYFRKERPDIVIAHMSRGAILPLI
                     SAFIARVPKRIYFNHGVPYIGYKNLPRMCFKLLEKLNCFFSNQVLTVSQGMIEPLTAI
                     TKKPVSILGPGSACGIDHNEFQRKSKYDIENIKQQYAASDTFIFTFVGRPVARKGFDI
                     TLKAFTKAFSGADNVILLLAGATSEDIFRVLGSKPNNIKPLGFIMDLDNVYLATDVVV
                     VPSEHEGFCYALLEGAARECALICSDIPGPDAIVINKRNGILITPKNIDSLMVSMSEL
                     FNDREMTKKYGEQAYMIASEFERKKIMLYYSYYIRSLFIEQR"
     gene            4972..6093
                     /gene="gmd"
     CDS             4972..6093
                     /gene="gmd"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-D-mannose dehydratase"
                     /protein_id="ABE98424.1"
                     /translation="MKKKALITGITGQDGSYLAEFLLDKGYEVHGIKRRASLFNTNRI
                     DHIYQDLHHEKQSFFLHYGDLTDSLNIVRLVQEIQPDEIYNLGAQSHVAVSFESPEYT
                     ADVDAIGTLRLLEAIRICGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KMYAYWITVNYRESYGIYACNGILFNHESPRRGETFVTRKITRALANIAYGLEPCLYL
                     GNMDALRDWGHAKDYVRMQWLMLQQETPDDFVIATGKQISVREFVRMAAANLGIELEF
                     IGCGLKEVGVVKAIHGNEAPSVKIGDVIVQVDPRYFRPAEVETLLGYPEKAKIKLGWE
                     PEITIEEMCKEMIRNDLNIAKQHSLLKLNGYDVSLSVEK"
     gene            6059..7063
                     /gene="fcl"
     CDS             6059..7063
                     /gene="fcl"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthetase"
                     /protein_id="ABE98425.1"
                     /translation="MGMMCPCQWRNNEMKMKKIYIAGHRGMVGSAIKRQLMMNSSVEL
                     IFRSSHELDLMNQDAVQNFFNTERIDEVYLAAAKVGGIYANNKYPADFIYNNILIQAN
                     VINSAHRAGVQKLLFLGSSCIYPKFAEQPIKEESLLCGSLEPTNEPYAIAKITGIKLC
                     ESYNRQYGRDYRSVMPTNLYGPNDNFHHENSHVLPALIRRFHEAKINNLPEVSVWGSG
                     NALREFLHVDDMAAASIYIMNLPNERYKEYTKPECSHINVGSGKDYSIRQLAEMVAEI
                     TNYKGNIYFDSTKPDGTPRKLMSNERLSKLGWSPKIELYDGVKNTYQWFLNNIENIRK
                     "
     gene            7150..8547
                     /gene="manC"
     CDS             7150..8547
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="ABE98426.1"
                     /translation="MLFPIIMAGGTGSRLWPLSRESFPKQYLKLYGDMTMLQSTIARL
                     NTLDCSTPLVICNEEHRFIVAEQLKKINKLDKNILLEPQGKNTAPAITLCAFAALKYA
                     KNDEDPLLLVLAADHVIGNISSFTKSVKSALPHAHAGKLVTFGIIPTTPETGYGYIHR
                     GEKFNDAYKVSSFTEKPDIYRAQEFLKTGEYYWNSGMFLFSAKRLLAEMEKYRPDIFS
                     ACEQAFLSPSNDLDFIRVDRDKFSLCPSESIDYAVMENTDDAIVVPMDAEWSDVGSWS
                     SLWDISDKDVNGNAVSGDLISFNSYNNFIKAEDKLVALVGVNDLVLVHTDDAILVSSK
                     YEVQNVKKIVEVLNQNGRDESKKHKEVYRPWGEYHYITEAEKYNVRRVLINPGQKIST
                     QTHNHRSEHWVIVSGIAKITINNDINIIKENESIYIPPGTMHTIENSEMFPLEIIEIQ
                     TGQLLMKMTLFVLKL"
     gene            8611..10050
                     /gene="manB"
     CDS             8611..10050
                     /gene="manB"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="ABE98427.1"
                     /translation="MSKKFNTLSIIRNSGVAFGTSGARGLVTEFTPEVCGAFSHAFIN
                     VMKQKYKFHGVALAIDNRPSSYSIAQACASVIAQYGLKVNYYGVIPTPALAYKALEDK
                     IPCIMVTGSHIPHDRNGLKFYSPIGEITKEDEIRIKDVSIDFTLDSSIPNLYPCPDAA
                     SCYFKRYIDFFPEGILKGKRVGFYEHSSAGRDLYIPIFEKLGAEIVPIGRSDKFIPID
                     TEAVGHDLVIQAKKWALEYKLDAIFSTDGDGDRPMLSDENGKWLRGDILGLLCAETLN
                     IKSIAIPISCNSLIMQRNCFEEVVLTKIGSPYVLDAFSSLKDKYHNYAGFEANGGFIL
                     GSDITVDNRKIKALPTRDALLPILVLLSANKKISSMVHSLPSRFTYSDRIANFLPEKR
                     DRLFTICKNNPHTIVDLLGENEAGIEVKDINNLDGYRFTLNNNVIIHLRSSGNAPELR
                     CYCEADSEFCAQKYVERILSSVACLIKIN"
     gene            10154..11638
                     /gene="wzx"
     CDS             10154..11638
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ABE98428.1"
                     /translation="MQNNKRVVFMNVSLNIMQQLINTVYTFIIPPLIIINYGSDINGL
                     ISSVKQCLVYLALVEAGVGGAAIVALFKPLSEGDRVLQSQIISEAKYFYSKIGVVFII
                     LLLLFSIIYAQIISDQININLAISLFVIMGLINAVDFLLISKYQVFLTADNKLYVTSA
                     TKILTIILDMGCAYILITEGINIILVQICSVFIYMIRYVIYYFYFLKKYSYISFDLKN
                     KTALLHQKSAVLIHQISGALVFNSPMILITIFLNLKAVSIYSIYMLIFMAIKVFLSTI
                     TNGMQSVFGRMIVNENFNAVREYFSKFEILYFSCLGWFYSCALILSYDFIYLYTLKFN
                     DPDYVQTILIYLFVIIGIADNIRVPGMTLIAAKGHYEQTKYRSIIEALLNIVFSIVFI
                     KIYGLPGVLLGSIISYSYRTIDIIYYSSNKILKQSIIFKMIMLFFLVFFILGNYFVVE
                     SLKMPFFINDFFKWILYSTFVAVLSALLWLLFYKIMRLMSDKIY"
ORIGIN      
        1 attggtagct gtaagccaag ggcggtagcg tgcataattg aaatgtacat caaattttat
       61 ggtttttctc taatggaaaa tataaccatt tacgattatg tgattgtaaa taacggtcta
      121 tttaataaag atgctgtaat cgtttttttt ataatatttt atttgtttgg ttgtcttatt
      181 aatattgtat ccaattatgt tagctgtatt tatgttctat tggtactaat accattatca
      241 ctattattcg cattaaatgg acctgataaa gcatcgtatt tgtctttata taatgcaata
      301 gatacagtga gcgatgtttt taaatattac aatacaatag attctccatt atactatata
      361 acaatggtct tatttaaaaa tatagggctt acattttaca gttttatggt agtgcaaaat
      421 ttaattatat cattttcatt atcatatcta atttataaat ataaatgggg gtatcacata
      481 gcaatgtggc tatattttac ccatctactc gtaccatttt cggttcgtgg acatttggtc
      541 ataattacca ttttatttgc aaatagaagt gtggtatcta aaattataag ttcgttagga
      601 ttagtggcga ttcatccact aatatctgca ttaccaatca cttatcaatt gcgtggcttg
      661 ataagaggta aacgtagtat tctaataatc gtgctaacga tatgtgcgct agtattttca
      721 tcatcctatt tgattagcaa gtatactagt tatgctataa acggtgatga gtatgttaaa
      781 aacgcggggt tatcactaat atatatagtt gatgcaatag agttattgct attttggtca
      841 ataattagac caatagcttt aatcgataag ttatttttac ttttcccggt cgtattaagt
      901 tttggtacaa taataatccc aatgcttggt cgattttcat atgcatgtga tttggtgtat
      961 attttaattt taattaataa cgcaaatagg ctaaaaaata aaaagacact ttttatgtgt
     1021 ttatcactta ttgcattaat ccgatttgtt aaaaattatt attcaattga gtattttata
     1081 ttatgatgct tttttttaaa aaccccaaaa ataaaaaatc aactggtatt ctatatctcc
     1141 agacgttatt acatgaaagg ttatcaaatg taagagtgat aacatcaacg ataatgatta
     1201 ttaaacgtta tttcaatata atgaaatcta cagaggtagc aatttttctt ccctttactt
     1261 tctcttgcat atttgcaaaa aaaaatgatt tggtaataat gcctgatgac tcaattcgat
     1321 caataaccag cgtttcaaaa gtgaggtatg aaaataaaaa aattttaaaa tggctatttc
     1381 agaatatcag gttaattata actaagaata taatatcttt aaaaaaagac gtggtctatt
     1441 taaccgtctc taaagacgat cagtggtcat tgcaatcaaa attcccaaag cataaaattg
     1501 tctatattcc tcatcctata caatctgcaa gtaataaaga agatgtgttg gatagatgcg
     1561 aaaatataag tactatatgt tttatgaatt tacaggaaca ttattctgtt aatccaagta
     1621 cttttattcg gcctaaaaca aaagttaatc tgaatgattt gcataataaa gttaatatta
     1681 tttttcatgg ttcttattct caagcttggt acgggaagta tatgtattat tatgggaaag
     1741 caaattgcaa agcagtaagg tatgtcgagg atttcgaaca gttctataat aatatagatc
     1801 taatcatctt gccattagca gctggcgcgg gaattaagaa tatcgtttta aattcagtat
     1861 acaggaataa acttatttta ggtaccgaag aggcattttc aggaatacct aaggaattta
     1921 caagaaaatt aattattgat tcgaatgatg acatcttaac aaaaattaac tcagaagtag
     1981 agtgtttagc tattaaggaa agttgtaata ttttacgtga ctatctagtc gttgaacaca
     2041 cagaaaaagc ttttatggat acaattttta aaaacataat ttaaagagca aatatgtcta
     2101 ttataagatt acaaggcgga cttggaaatc aactttttca gttctcattt ggatatgcgc
     2161 tttccaaaat taatgggaca ccattgtatt ttgatataag tcattatgct gaaaatgatg
     2221 atcatggtgg ttacaggcta aacaatctcc aaattccaga ggaatattta cagtattaca
     2281 caccaaaaat taataatatt tataaattat tggttcgtgg gtcaagatta tatcctgaca
     2341 tctttctttt tttaggtttt tgcaatgaat ttcatgccta tggttatgat tttgaatata
     2401 tagcgcaaaa atggaaatcc aaaaaatata tagggtattg gcaatctgaa cactttttcc
     2461 ataaacatat attagatcta aaagaatttt ttattccaaa gaatgtgtct gaacaagcaa
     2521 atttacttgc agcgaaaatt cttgaatctc aatcatcact ttctattcat ataagaagag
     2581 gagattatat aaaaaacaaa acagctactt taactcatgg cgtttgttcg ttagagtatt
     2641 acaaaaaagc tttaaataaa atacgcgatt tggcaatgat acgtgacgta tttattttca
     2701 gtgatgatat tttttggtgt aaagaaaata tcgaaacatt actcagtaaa aaatataata
     2761 tatattattc agaagattta tcacaagaag aagatttatg gttaatgagc ttagctaacc
     2821 atcatattat agcgaatagt agttttagtt ggtggggggc ttatttaggt tcatcagcgt
     2881 cacagattgt tatttatcct actccttggt acgatataac tccaaaaaat acttatatcc
     2941 ccatagtcaa tcactggata aacgtggata aacatagctc gtgttaggga tagattataa
     3001 aggagtttgt agtggagaga ccatattttt cgtttttatt atgcgttaac aaagatgaag
     3061 gttttctcaa tgatgctctt gagagtatta taaaccaaga ctattcttat gaatatgaaa
     3121 ttattgttat tgcaaataat tgtgatgata atctatgggc atatttgaac tccgttgaga
     3181 gtgacaaatt gaaaatacat cgtaccaata ttgggcagtt agcttttaat ttaaactatg
     3241 gagcaaatat tgctagaggg gagtatattg ttaggatgga tgccgatgat gtatccaccc
     3301 cgaatcgatt aaagattctt gaggcggcta ttcaagaaaa taactatcca gatgtaatag
     3361 gaacttcagt ttattttata agtgagacag gtagaatcat aaagaaatat gttcctcctt
     3421 taggagaggt aaaaagtgct ctcattaaaa atccattcgt tcacccaact gtggccatca
     3481 aaaaagtgtc attatttaaa attgggggct atcttggtgg atttcagtca gaagataatg
     3541 atttatggat tcggatgctt agagcgcatg aatttaagtt agtaaatatt gattctgtaa
     3601 cattatttta tagaataaac gatcatcaaa ctcgtggtcg agttctacca tatgcagaag
     3661 ttgcgtctta tgcgttgaga gagtttcttt taaaaaagag aataaaatat tttgcggcgc
     3721 tacttatcaa tattggaaag gcgcttatac ttccaaaaag ataaggtatg aaacttatga
     3781 aaataatgca tacggtatta aatacattta ctgcaaagaa gtttgttgag ccgcttttag
     3841 ataatgttaa tgagcaacac aataaagaaa aaataattca taaaattttg attgatcctg
     3901 ttaatggtga tgagcgtttt ttaaaagaac ttcatgtaca atatgaaata aaaaaatttg
     3961 atatagatta tttatttatc aaacatataa ttgagcttta tttctatttt agaaaagaaa
     4021 gaccagatat agttatagcc catatgtcaa gaggggctat tctaccgtta atttctgctt
     4081 ttatagcccg agtacccaaa cgtatatatt ttaatcatgg ggtgccatat attggttata
     4141 agaaccttcc taggatgtgt tttaaattac ttgaaaagct aaattgtttt ttttctaacc
     4201 aagtattaac tgtttctcaa ggtatgattg agcctttaac cgctataact aaaaagccag
     4261 tatcaatact tgggcctggc tctgcttgtg gtattgacca caatgagttt caaagaaagt
     4321 caaaatatga tatagaaaat ataaaacagc aatacgctgc atcagatact ttcatcttta
     4381 ccttcgtagg aaggccggtt gcacgaaaag ggtttgatat aacgctgaaa gcgtttacta
     4441 aggcatttag tggtgcagat aatgtaattc ttttattagc aggggcaact tcggaagata
     4501 tttttcgcgt attaggaagt aaacctaata atatcaaacc attaggtttt ataatggatt
     4561 tagataacgt atatttagct acagatgtag ttgtcgtacc aagtgagcat gagggttttt
     4621 gctacgcact tttagaagga gctgcaagag agtgcgctct catttgtagc gatatccctg
     4681 ggcctgacgc aattgttatt aataaaagaa atgggatttt aataacccct aaaaatattg
     4741 atagccttat ggttagtatg tctgaattat ttaatgatag agaaatgaca aaaaaatatg
     4801 gagaacaagc atatatgata gcttccgaat ttgaacgaaa aaaaatcatg ttatattata
     4861 gttactacat aagaagttta tttattgaac aaaggtgatt tttaaatgta attatccgta
     4921 tacaactaat actgatataa ttttttgttt aatctcatta gcaggtattt tatgaaaaag
     4981 aaagctctga ttactggaat tactggacaa gatggttctt atctggccga atttttatta
     5041 gataaaggtt atgaagttca tggcataaaa agaagagcct ctctttttaa cactaataga
     5101 attgatcata tttatcagga tctacatcat gagaaacaaa gtttttttct tcattacggt
     5161 gatcttaccg attcgttgaa tatagttaga ttggtacaag aaatacaacc tgatgaaatt
     5221 tataacttag gggcacaatc gcacgtagca gtatcatttg aatcaccaga atatacagcc
     5281 gatgttgacg caattggtac gttacgattg ttagaagcta ttcgtatatg tgggcttgag
     5341 aaaaaaactc gtttttatca agcctctacc tcggaattat atgggttagt gcaagaaata
     5401 cctcaaaagg agactacacc attttatcca aggtcacctt atgcagtagc taagatgtat
     5461 gcatattgga ttacagtcaa ttatagagaa tcttatggga tatatgcttg taatggaatt
     5521 ttatttaatc atgaatcacc tcgccgtggt gaaacatttg taacaagaaa aatcacccga
     5581 gcattagcaa atattgcgta cggattagag ccatgtttgt atttaggcaa tatggatgct
     5641 ttgcgcgatt gggggcatgc aaaagattat gtaagaatgc aatggttaat gttacaacaa
     5701 gaaacacctg atgatttcgt aatagctacg ggcaagcaga tctctgtaag agagtttgtg
     5761 agaatggctg ctgctaattt aggtattgag cttgaattta ttggttgtgg tttgaaagaa
     5821 gtaggagttg tgaaagcaat tcatggaaat gaagctcctt cggttaaaat tggcgatgtt
     5881 attgttcaag tggatcctcg ttattttcga cctgccgaag ttgaaacttt gctcggttat
     5941 cctgaaaaag ccaaaataaa actagggtgg gaacctgaaa taacgatcga agaaatgtgt
     6001 aaggaaatga taagaaatga tcttaacata gcaaagcaac attctctgtt aaagttaaat
     6061 gggtatgatg tgtccttgtc agtggagaaa taatgaaatg aaaatgaaaa aaatatatat
     6121 tgcagggcat agagggatgg ttggttctgc aatcaaacgt cagttaatga tgaatagttc
     6181 tgttgagtta atttttcgta gtagtcacga acttgacttg atgaaccaag atgctgtcca
     6241 aaattttttt aatacagaaa ggatcgatga agtctattta gcagctgcta aagttggtgg
     6301 gatatatgcg aataataaat atccagctga ctttatatat aataatatac tcatacaagc
     6361 caatgtaatc aattcagctc atagagctgg ggttcaaaaa ttattatttt taggctcatc
     6421 atgtatctat cctaaatttg cagaacaacc tataaaagaa gaatccctac tttgtggttc
     6481 attagaacct acaaatgaac cttatgcgat tgcgaaaata acagggatta agttgtgtga
     6541 gtcttataat agacaatatg gtcgtgatta taggagcgtt atgcctacaa atctttatgg
     6601 gcctaatgac aattttcatc atgaaaattc acatgtatta cctgccttaa tacggcgttt
     6661 ccacgaagca aagattaata atcttccaga ggtcagtgtt tggggttctg gcaatgcgtt
     6721 acgagaattt cttcatgtag atgatatggc agctgcatcc atatatataa tgaatttacc
     6781 taatgaacgt tataaagaat atactaaacc agagtgctca cacataaatg ttggttctgg
     6841 taaagattat tcaattcggc aattggcaga aatggttgcg gaaattacta attataaagg
     6901 aaatatttac tttgatagta ctaaacctga tggaacacct cgtaaattaa tgagcaatga
     6961 acgattgagt aaactaggct ggtcacccaa aatcgagtta tatgatggag taaaaaatac
     7021 ttaccagtgg tttttgaata atatagaaaa tataagaaaa taaaaacctt acataaaaaa
     7081 ttatgggtag gtcctcctgg aagaaatatt ttataaatga catttatatt tgtacagagg
     7141 ttatagtaga tgctttttcc gataattatg gctggtggca ccggtagtcg actttggcca
     7201 ctgtctcgag agtcttttcc aaagcaatat ctaaaacttt atggtgacat gacaatgtta
     7261 caatctacaa ttgcgagatt aaatacatta gattgttcga ccccattagt catttgtaat
     7321 gaagagcata gattcatcgt tgcggaacag ttaaagaaaa ttaataaatt agataagaat
     7381 attctgcttg aaccacaagg taaaaatacg gccccagcaa taaccttgtg tgcgtttgct
     7441 gctttaaaat atgcaaaaaa tgatgaggat ccattactac ttgtattggc tgctgatcat
     7501 gtaataggaa acatatcttc ttttactaag tcagttaaaa gtgcattacc gcatgctcat
     7561 gctggcaagc ttgtaacatt tggtataata cctacaacac ctgaaactgg ttatggatat
     7621 attcaccgag gagaaaaatt taatgatgcg tacaaagttt catcttttac agaaaagcct
     7681 gatatttatc gtgcgcaaga atttttaaaa acaggtgaat attattggaa cagtggtatg
     7741 ttcctattta gcgctaaacg actgcttgca gagatggaaa agtatcgccc agatattttt
     7801 tctgcatgtg aacaagcttt tttatcgcca tctaatgatc tagattttat tcgtgtggat
     7861 cgtgataagt tttcgttatg tcctagtgag tcaattgact atgctgtgat ggagaatacg
     7921 gatgatgcca tcgttgtacc tatggatgca gaatggtctg atgttggttc gtggtcttca
     7981 ttatgggata tttcagataa ggatgtaaat gggaatgctg ttagtggtga tttgattagt
     8041 tttaactctt ataataactt tattaaagct gaagataaac tagttgcttt ggttggcgtt
     8101 aatgatttag tcttggtgca tacggatgat gctatattag tgtcaagcaa atatgaagtg
     8161 caaaatgtaa aaaaaatagt tgaagtcctt aaccaaaacg gcagagatga gtcaaaaaaa
     8221 cataaagaag tatatcggcc ttggggtgaa tatcattata ttacggaggc agagaaatac
     8281 aacgttagac gcgtactaat taatccaggt caaaaaatct caacgcaaac acacaaccat
     8341 agatcagagc actgggttat agtttcagga atagcaaaaa ttacaattaa taacgatata
     8401 aatataatta aagaaaatga atctatttat atacctcccg gtactatgca taccatagaa
     8461 aactcagaaa tgtttccatt agaaataatt gaaattcaaa ccggtcaatt attaatgaag
     8521 atgacgttat tcgtccttaa actttaatgt aaaagagaaa tgaatatttg taattataag
     8581 tagttattaa gctaatttta atgaggtttt atgagcaaga aatttaatac tttatctatc
     8641 atcaggaata gtggcgttgc ctttggtacc agtggtgctc gaggcctcgt tacagaattc
     8701 acccccgaag tatgcggagc tttttctcat gcttttatta acgtaatgaa gcaaaagtac
     8761 aagtttcatg gagtcgcatt agcaattgat aatcgcccta gtagttactc tatagctcaa
     8821 gcatgtgcat ctgtgattgc gcaatacggc ttaaaggtaa attattacgg tgttatccca
     8881 acaccggcat tagcttataa ggcactggag gataaaatac cgtgtataat ggtaacgggt
     8941 agtcacatac cacatgatcg taatggatta aagttttata gcccaattgg cgagatcact
     9001 aaggaagatg aaattcgcat taaagatgtt tctatagatt ttacattgga ctcatccata
     9061 ccaaatttat atccgtgtcc tgacgcagct agttgttatt ttaaaagata cattgacttt
     9121 tttcctgaag gaattttaaa aggtaaaaga gttggctttt acgagcattc aagtgctggg
     9181 cgtgacttat acattcctat ttttgaaaag ttaggagcag agatagttcc catcggtaga
     9241 agtgataaat ttatcccaat agatacagaa gctgttggtc atgatcttgt tattcaggca
     9301 aaaaaatggg cgttagaata taaattagat gctattttta gtactgatgg cgatggcgat
     9361 cgcccaatgt taagtgatga gaatggaaaa tggttgcgag gagatatact tggactgtta
     9421 tgtgctgaaa cattgaatat aaaatctatt gcgattccaa ttagttgtaa ctccttaata
     9481 atgcaacgta attgttttga agaagttgtt ttaacaaaaa ttggttcgcc ttacgtatta
     9541 gatgcttttt cttctttaaa agataaatat cataattatg caggttttga ggctaatggt
     9601 ggttttatat taggttcgga tataacagtt gataatagaa agatcaaagc cttacctaca
     9661 cgagatgcat tactccctat attagtttta ctttctgcaa ataaaaaaat ttcaagcatg
     9721 gtacatagct taccaagtag atttacttat tctgaccgaa ttgctaattt tcttccagaa
     9781 aaaagagata ggttatttac aatatgtaaa aataatccac atacaatagt tgatcttttg
     9841 ggagagaatg aggcgggcat tgaagttaaa gatataaata atttagacgg ttatagattt
     9901 acactaaata ataatgtcat cattcatcta agatcttccg gaaatgctcc agagttgaga
     9961 tgttattgtg aggctgacag tgagttttgt gctcaaaaat atgtggagag aattttaagt
    10021 tcggttgctt gccttataaa aataaattaa tattttaagg ttgtttagtt tttagtgcag
    10081 tttcaccttt tcatgactgc actatcattt tatatcaaaa cttaacaagc tagacagcaa
    10141 ggttgacgag gcaatgcaga ataataaacg cgttgtattt atgaatgtgt ctctaaatat
    10201 aatgcagcaa ctgattaata cggtatatac ctttatcatc cccccattaa ttattataaa
    10261 ttatggttcg gatataaatg gtttgatttc ttctgttaaa caatgccttg tatatttagc
    10321 tctcgtagag gctggtgttg gaggggcagc tattgtagca ctattcaaac cattgtcaga
    10381 aggggatcgt gtgctacaaa gtcagatcat atcggaggct aaatattttt actcaaaaat
    10441 tggtgttgta tttattattt tacttttatt attttcaata atatacgcac aaataatttc
    10501 tgaccaaatt aacatcaatc tagcaattag tctttttgtt attatggggc taataaatgc
    10561 ggtggatttt ttattaatat caaagtatca ggtttttctt actgctgata ataaattata
    10621 tgtaacttcg gctacaaaaa tattaacaat catattagat atggggtgtg cttatattct
    10681 gataactgaa ggtattaata ttattttggt gcaaatttgc tccgtgttta tctatatgat
    10741 tagatatgtt atttattatt tttattttct gaaaaaatac agttatatat catttgatct
    10801 aaaaaataaa actgcattgc ttcatcagaa atcagcagtt ttaattcatc agatatcagg
    10861 agcattagtt ttcaatagcc caatgatatt gattactatt tttcttaacc taaaggcagt
    10921 aagtatatat tctatttata tgcttatatt tatggccatt aaggtttttt tatcgaccat
    10981 taccaatggt atgcaatccg tattcggccg tatgattgtt aatgaaaact ttaatgctgt
    11041 aagagaatat ttttcaaagt ttgaaatttt atatttttca tgtttgggat ggttctattc
    11101 atgtgctttg attttatcgt atgattttat atatttatat actttaaaat ttaacgatcc
    11161 tgattatgtt caaactatat tgatttatct ttttgttatt ataggtatag ctgataacat
    11221 tcgcgtccca ggaatgacat taatagcggc taaaggtcac tatgaacaaa caaaatacag
    11281 atctattatt gaggctttat taaatatcgt tttttcaata gtttttatta aaatatatgg
    11341 acttcctggt gtattgctag gttccattat ctcttattca tacaggacaa tagatattat
    11401 ttattatagt agcaataaaa ttttaaaaca atcgataata tttaaaatga ttatgttatt
    11461 ttttttggtt tttttcattt tgggtaatta ttttgtcgtt gaaagtttga aaatgccatt
    11521 ttttataaat gattttttta aatggatttt atattcaact tttgttgcag ttctatcagc
    11581 tttgttatgg ttattgtttt ataaaattat gaggctaatg agtgacaaaa tttattaaat
    11641 tttttatgcg taaattgtaa cttttatatt gcatactggt atattttaaa ggttttcata
    11701 caagccgcta cttatatgcg gtaaccccct gacaggagta aacaatgtca aagcaacaga
    11761 tcggcgtcgt cggtatggca gtg
//

</pre>