<pre>LOCUS       KP835694               12911 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain 2745-53 serotype O125ab:H19 O-antigen gene
            cluster, complete sequence.
ACCESSION   KP835694
VERSION     KP835694.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12911)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12911)
  AUTHORS   Yan,X., Baranzoni,G.M., Fratamico,P.M., Debroy,C., Tebbs,R.S.,
            Liu,Y., O'Connell,C.D., Swimley,M. and Matheny,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2015) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. V. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2745-53"
                     /serotype="O125ab:H19"
                     /db_xref="taxon:562"
     misc_feature    1..12911
                     /note="O-antigen gene cluster"
     gene            101..1372
                     /gene="wzx"
     CDS             101..1372
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AKM71197.1"
                     /translation="MKVYNVFILNGLSVFVKMATLLGLNKVFAIYLGTSGYALIGQIQ
                     NILNIAMVAPSQAISTSIVKYTSEYKNSNKEKIWSFWINAIYLSTIMGFITAVIIIIF
                     KNIIIEQFFNSNDKVSIAIYFLGGSVILYAWNNIILSMLNGLSFIKNYIWINIISSLI
                     SAIYAFILTISFGMRGALIAVVSNQAILFFVTFFYSKKYLREYYVNNCNRIDISILKR
                     IIIFSLMTVTSAILVPVSTLYVRNEIITSINIEYAGLWDAVNRISSVYLMLITTTLGV
                     YFFPRFSSASNRSELLNEIKVCYLYLVPLVCISSVLMYTFRGLIVNILFTHEFVPIVD
                     VIVYQLIGDFFKIIAWVLGYLLISKAFVKEYIVLEVLQWSVYCILSKYSLCYVENAQI
                     SYVFKAYIISQALYMFSLIVTICYLLNKNKV"
     CDS             1379..2299
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="AKM71198.1"
                     /translation="MINKNKVSVIIPVYNAEKYLGACIDSILEQTYKIFEIIIINDGS
                     QDGTQKILDEYKNKYPSIFKIKHRENKGIVATLNELLDMATGVYIARMDADDIAHRER
                     LRLQVEIMDADQKIACCGTNIKVFESAVNSGKIIKMPNSDEACKIGLLVGSCFAHPTV
                     MMRSSIIKKECLYYKPRYQYAEDYEFWSRLANYGKFCNINIPLLYYRNHGNQISNIKK
                     HIQADIHISIAIDNLKKQGIILDKDELGMVLFPQSSHRFLNAICYYFKVYFIIFSKLG
                     VKIKYINGVFLRKFAYYCKIKINSILKKGY"
     CDS             2301..3146
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="AKM71199.1"
                     /translation="MNNFYKISLIGTTQGGRDKEIERLLNSIASSTYKNLEIIFIDQT
                     DDIIIEQLFLKHAKNIKYKLVKSNKVPLSTARNQGLKLCSGNVIGFCDDDAFYAPDFF
                     EKVIQSNIKKETVLSVPVLDFQTMKSYANRVYPKTKKEMGYNQIIKSSLSVGTFIFTH
                     KLDNIYFDERLGVGTLFGGSEETELFFRLKSLGFRVCFQPIFGVYHDNDLYCEGIPEK
                     YRKYAVGYGVVIKKYLVKSRYLLLLEIFRITVKSFFGIVLKKQKKLYMYRLIGFWTGL
                     LKYDL"
     gene            3136..4458
                     /gene="wzy"
     CDS             3136..4458
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AKM71200.1"
                     /translation="MIYKQYKLVLAQFYGFIGLIMIAVLSTSVKVEIDAILIWFLSVI
                     FTIAIIFFVYNRMWVWFDGMFIILSPFYFFVSFLLYYFFGVNVFNVENNIINTSFLYC
                     VAVWCIVASISLIRPHGINIFLLNKQYNYDNNYNLFIPSCIAWLISLFFLYQSRNLSL
                     NALGESTRLELINSVSQSGWYLKYVVIAYFWILLDLKVVKKNLTNYSFVIYSIPVLLY
                     FYTLMLVGSRREIALSFIMAVLLSLYKAKFKIPLKVALFSILFVSTIILFGIYRSDYN
                     VESSIRLLNALGEFIYPISTLQYYSSIHYQTQFFGISYLQFIVNFIPKELFMGLKPDT
                     LAIQFAKEVYLPSQEFMMGYAFTPFTEAFINYNYYGVIIFPAILCGYSYIMERLIGNN
                     YFLYLVFLSQSINFQRSEFSSMIFELGMFFMAYICLRLSMQIVFFKFK"
     CDS             4468..5481
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="AKM71201.1"
                     /translation="MTILVTGGAGYIGSHTVLSLLEQNEDVVIIDNFSNSTEDIIKVI
                     SKLSGKKIRAYKVDLLDKKELKTIFTNHTFSKVLHFAGLKSVSESVQSPIRYYMNNIV
                     GLLNLLELMKIFHVNKIIFSSSATVYGLPETIPINEKCRIGETTNPYGTSKYYSEVIL
                     KDASLANPKLDVIILRYFNPVGAHESGLIGENPRGTPANLVPYMMNVALGKLPALTIY
                     GHDYPTPDGTGIRDYIHVNDLAEGHISAFKMFGKGGNYKIYNLGTGRGYSVLEVIKTF
                     QSIIGKNINYSFGPNRSGDVAECWADVSKAEKELDWKARRDLTEMLSSAWKWHTLNPD
                     GYN"
     CDS             5497..6525
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol 3-phosphate glycosyltransferase"
                     /protein_id="AKM71202.1"
                     /translation="MLYLDGIIYSLQSYGGISVYYNQIIKAIKATNIEYTILNYENTG
                     CQFISEDVRQPGRYINLYPRFLERYRAVDIPENSLLHSSYYRFNKGKKNKNITTVHDF
                     TYEKYMSGLRKYVHCHQKYNAIKNSDKIICISENTANDLLRYCPVPESKIEIIHNGVS
                     DNFYELKNVNYNDDDNFMLFIGSRKAYKNFQLAVEAINKLEGFKLYIVGGGPLGKAEK
                     FLLDKYIPYRYIHYPYLTEQELNLKFNQAHSLIYPSSYEGFGIPLLEAMRAGCPFIAY
                     NHSSIPEVAGDAGYLMDELSISNLIDGISFINRNRNQIIKKGLSQAQRFSWKKCTDKT
                     IDIYKKLL"
     gene            6536..7657
                     /gene="gmd"
     CDS             6536..7657
                     /gene="gmd"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="AKM71203.1"
                     /translation="MTKKALITGITGQDGSYLAEFLLNKGYEVHGIKRRSSIFNTGRI
                     DHIYQDPHNINLKLHLHYGDLTDTSNLTRILSEVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAIGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEVPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANISQGIEKCLYL
                     GNMDSLRDWGHAKDYVRMQWMMLQQDQPEDFVIATGKQISVREFVRMSAKEVGLELEF
                     SGTGVDEIAMVVNKTSDCAVGVNIGDVIVRVDPRYFRPAEVETLLGDPTKAKNVLGWE
                     PEITVEEMCAEMVASDLEKAKQHALLKSHGFDVAVSLER"
     gene            7661..8626
                     /gene="fcl"
     CDS             7661..8626
                     /gene="fcl"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="AKM71204.1"
                     /translation="MTKKRIFVAGHRGMVGSAICRQLLARNDVELIVKTHKELDLTVQ
                     KDVECFFEQERIDQVYLAAAKVGGIHANNTFPAEFIYQNLLIESNIIHSSYKAGIKKL
                     LFLGSSCIYPKFAEQPMKESALLTGTLEPTNEPYAISKIAGIKLCESYNRQYGCDYRS
                     VMPTNLYGMNDNFHPNNSHVIPALMRRFHEAKELGLNEVVVWGTGTPKREFLYVDDMA
                     AASVYVMELDDEIYKKNTQPMLSHINVGTGVDCSIREMAETMALVVGYDGKIVFDITK
                     PDGSPRKLMDVTRLENLGWKYRYNLKQGLELTYKWFINNLDSFRS"
     CDS             8629..9084
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="AKM71205.1"
                     /translation="MNKRLETELFKSIVEHTPLISIDLIIRNEDGKALLGQRLNRPAQ
                     NYWFVPGGRILKDESFENAFKRVTFEELGVQISINEAKFLGIYEHFYSDNFSGTCFST
                     HYVVHGYEISLMPHQINYPTLQHSTYNWFDIAELLANSSVHQYTKNYFK"
     gene            9096..10481
                     /gene="manC"
     CDS             9096..10481
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase 1"
                     /protein_id="AKM71206.1"
                     /translation="MLLPVVMAGGSGTRLWPLSRTLYPKQFLSLTSRLTMLQETLRRL
                     EGVEHRPALVICNEVHRFIVAEQMRNVNLANSGILLEPIGRNTAPAVALAALKAISSG
                     EDPILLVLAADHEIQDQKRFISSILAAKEFAEEGKLVTFGIVPTKPETGYGYIKTGEN
                     LNEYGFKVSAFVEKPELDVAKKYLEDGDYLWNSGIFMFRASVFIDELNKFRPDILKIC
                     QQALKFSTQDLDFIRIDNDSFSCCPEESIDYAVMEKTTEAVVVPLNAHWSDVGSWSAL
                     WEISRKDKSGNAIRGDVLIHDSSDSYLYSQHRLIGVVGVKDLVVVETKDAILVAHKDK
                     VQQVKNIVEKLKVNNRTEYLQHREIFRPWGSHDSIAEGSRFEVKHVVIHPGHKTAKQI
                     HYHRTEHWIVVSGTAKVHYEDEIFLVSENESTYIPIGVPHFIENPGKIPLEIIEVRSG
                     VYLDEDDVVRI"
     CDS             10565..11311
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="AKM71207.1"
                     /translation="MKVSIITVTYNSEKTLRDTLESIELQTYKDIEYIIIDGGSTDNT
                     LKLINEVSTRVTKCLSESDNGIYDALNKGINLSTGDIIGFVHSDDLLARPDIIETIVS
                     RFHETKADVVYGDLVFFEKNQIDIIKRYWRSGPFKRSKLSLGWAPPHPSFYMRRELYK
                     DDGYFDLSYRIAADYDQMVRILKRDDIKVIYVPQVFVKMRLGGESTRIDNAISSTKEI
                     VEVMKNHNVNWKIAIIIRKISKLMQLFAHK"
     CDS             11336..12706
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="AKM71208.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
ORIGIN      
        1 ctggtagctg tatagccagg ggcggtagcg tggtttatat ttatggtata attaaatatg
       61 agttttgtta tcataaccta cttaatatgt taggtgaact gtgaaagtat ataatgtctt
      121 tattcttaat ggcctttctg tatttgtcaa aatggcaact ctacttggac ttaataaagt
      181 attcgcaata tatttaggta cgtctggata tgcacttatt ggccaaatcc aaaatatcct
      241 taatatagct atggttgcgc catcacaagc tatttcaacg agtattgtta agtatacgtc
      301 agagtataag aatagtaata aggaaaaaat ctggagtttt tggattaatg caatttattt
      361 gtctactata atgggattta taaccgctgt tatcataatt atctttaaaa atataattat
      421 cgaacaattt ttcaattcta atgataaagt aagcatcgca atttatttct tgggagggag
      481 tgttatttta tatgcatgga ataatataat tctttctatg ttaaatggat tgtcatttat
      541 taaaaattat atatggatta atatcatatc gagtttaata tctgcaatat acgcctttat
      601 attgacaatc tcttttggta tgagaggagc attgattgct gtagtttcaa atcaagctat
      661 attgtttttt gtgacttttt tttactcaaa aaaatattta agagaatact atgttaataa
      721 ttgcaatcga attgatattt caatattaaa acggatcata attttttctt taatgacagt
      781 cacttcagct atattagtgc cagttagtac gttatatgtg agaaatgaaa tcataacatc
      841 aataaatatt gagtatgctg ggttatggga tgctgttaat agaataagtt cagtctattt
      901 gatgttaatt acgactacat tgggggtata tttctttcca aggttttcaa gtgcatctaa
      961 ccgttcagaa ttgcttaatg aaataaaagt ttgttatttg tatttggttc cgttagtttg
     1021 tatatcttca gtcttgatgt atacatttcg cggtttgata gtaaatattc tatttacaca
     1081 cgagttcgtt cctattgttg atgttatagt ttatcaacta attggagatt tttttaagat
     1141 aattgcatgg gtgcttggtt atttgctgat tagcaaagca ttcgtaaaag aatatatagt
     1201 gttagaagtg ttacaatggt ctgtatattg tatattaagt aaatattcgt tatgctacgt
     1261 cgaaaatgct caaatttctt atgtttttaa agcgtatata atatctcaag cattatatat
     1321 gttctcctta atagtaacga tctgttatct gcttaataaa aacaaagtat gatatattat
     1381 gattaataaa aacaaagtat ccgttataat accggtatat aatgctgaaa aatacttagg
     1441 cgcatgcatt gacagtatat tggaacaaac atataaaata tttgaaatta tcatcatcaa
     1501 tgatggctcg caggatggta cacaaaagat acttgatgaa tataaaaata aatatccctc
     1561 gatattcaaa attaagcata gagaaaataa agggatagta gctactttaa atgaattatt
     1621 agacatggct acgggagtgt atattgctag aatggatgct gatgatattg ctcacagaga
     1681 aaggttgaga ttacaagttg aaataatgga tgcagatcaa aaaatagcgt gttgtggaac
     1741 taatatcaaa gtttttgagt cagctgttaa tagcggaaaa attataaaaa tgcctaactc
     1801 agacgaagcc tgtaaaatag gattgttggt gggcagttgt tttgctcatc caactgtaat
     1861 gatgagaagc agcattataa aaaaagaatg cctatattat aagccacgct accaatatgc
     1921 tgaagattat gagttttgga gtagattagc aaactacgga aaattctgta atataaatat
     1981 acccttgctt tattatcgaa atcacggaaa tcaaatatcg aatataaaaa agcatataca
     2041 agcagatatt cacatatcta ttgccattga taatttaaaa aaacagggga ttattctcga
     2101 taaagatgag ctagggatgg ttttgtttcc gcaatcaagt catcgttttc ttaatgctat
     2161 ttgttattat tttaaggtgt attttattat tttttctaag ttaggagtaa agattaaata
     2221 tataaatggt gtttttctta gaaaatttgc ttattattgt aaaataaaga ttaattcaat
     2281 cttaaaaaaa ggatattaat atgaataact tttataaaat tagtttgata ggcacaactc
     2341 aaggtggaag ggataaagaa attgaacgac tgctaaatag catagctagc agtacgtaca
     2401 aaaacctaga aattatattt attgatcaga ctgatgatat cataattgag caattatttt
     2461 taaaacacgc aaaaaatata aaatacaagt tggttaaatc aaataaagta ccactttcaa
     2521 ctgctcgtaa tcaagggctt aaactatgca gtggtaatgt tattgggttc tgtgatgatg
     2581 atgcttttta tgctcctgat ttttttgaga aggtgattca atcaaatatc aaaaaagaaa
     2641 ccgttctttc agttcctgta ttagattttc aaacaatgaa atcttatgcc aatagagttt
     2701 atcctaaaac taaaaaggaa atgggttata atcaaataat taaatcgtca ctaagtgtgg
     2761 gtacttttat ttttactcat aaactcgata atatatattt tgatgaaagg ttaggtgttg
     2821 gtactttatt cggtggatcc gaagaaacgg agttattttt tcggctgaaa tctcttgggt
     2881 ttagagtatg ttttcaacct atatttggag tttatcacga taatgattta tattgtgaag
     2941 ggataccaga aaaatacaga aaatatgcag ttggatatgg tgtcgtgata aagaagtatt
     3001 tagtgaaatc tcgttattta ttactattgg aaattttcag gatcacagta aaatcatttt
     3061 ttggtatcgt tttaaaaaaa caaaaaaaat tatatatgta cagattaatc ggtttctgga
     3121 cgggattgtt aaaatatgat ttataaacaa tacaaactgg tattggctca attttatggc
     3181 tttattgggt tgataatgat tgctgtattg agcacttctg tcaaagttga aattgatgct
     3241 atcctaattt ggtttttatc tgtaatattt acaattgcaa ttattttttt tgtatataat
     3301 cgaatgtggg tatggtttga tggaatgttt ataatattat cgccttttta tttttttgtg
     3361 tcttttttgt tgtattattt ttttggtgtt aatgtattta atgttgaaaa taatataatc
     3421 aacacttcat tcttatattg tgttgctgtt tggtgtatag ttgcatcgat tagtttgatt
     3481 aggcctcacg gtatcaatat atttctttta aacaaacaat ataactatga caataactat
     3541 aatctattta ttccatcatg catagcatgg ttaatatcat tattttttct atatcaatca
     3601 agaaatcttt ctctgaatgc tttgggcgaa agtactcgcc tagagttaat taattcggta
     3661 agtcaaagtg gatggtatct gaaatatgtg gttattgctt atttttggat tttacttgat
     3721 ttaaaggtag taaaaaaaaa tctcacaaat tatagtttcg taatatacag catacctgtg
     3781 cttttatatt tttatacttt aatgctggta ggttcaagac gagaaattgc tttatctttt
     3841 attatggctg tattgctatc actttataaa gctaaattta agataccatt aaaagtagcg
     3901 ttgttcagta ttctatttgt atcaacgatt atactttttg ggatatatcg ttcagattat
     3961 aatgtcgaat caagcataag attattgaat gctttgggtg aatttattta tcctatatct
     4021 acacttcaat attattcttc gatacattat cagactcaat tttttggtat atcctatctt
     4081 cagttcattg taaactttat accaaaagaa ttatttatgg gattaaagcc agacacatta
     4141 gctattcaat ttgcaaagga agtttatttg ccaagtcaag agtttatgat gggatatgca
     4201 tttactcctt ttacagaagc ttttattaat tataattatt atggtgtaat tatatttcct
     4261 gctattttat gtggttattc atatataatg gaaaggttaa ttgggaacaa ctattttctt
     4321 tatcttgttt ttctttccca atctatcaat ttccaacgct cagagttttc ttctatgata
     4381 tttgaattag gtatgttctt tatggcatac atatgcttga gattatcgat gcaaatagtt
     4441 ttttttaaat tcaaataagg tgtttttatg acaatattag ttacgggtgg agctggatat
     4501 attgggtccc atactgttct gtcattattg gagcagaatg aagacgttgt catcatagat
     4561 aatttttcta attctacaga agatatcatc aaagtcattt caaagcttag tggtaagaaa
     4621 atcagagcat ataaggtaga tttactggac aaaaaagaat taaagactat attcacgaat
     4681 cacacattct ctaaagtatt acattttgca ggccttaagt ctgtctcaga atcagttcaa
     4741 agtcctatac ggtattatat gaataatatt gttgggcttt taaatttgtt agagttaatg
     4801 aagatctttc atgtaaataa aattatcttt agctcatctg ctacggtgta cggtcttcca
     4861 gaaacgatac ctatcaatga aaaatgtcgt ataggtgaaa caacgaatcc atacgggacc
     4921 agtaaatatt actccgaggt tatattaaaa gatgcttctc tggcaaaccc taaattagat
     4981 gtgattattt tgcgatattt taatccagtg ggggcgcatg agtctgggtt aataggtgaa
     5041 aatcccagag gtactcctgc taacttagtt ccatatatga tgaatgttgc tttaggtaaa
     5101 ttacctgcgc ttactattta tggtcatgac tatccaacgc ctgatgggac tggtatcaga
     5161 gattatattc atgtaaatga tttagctgag gggcatattt cagcatttaa aatgtttggc
     5221 aaaggaggaa attataaaat atataactta ggtacgggta gaggatattc tgtattagaa
     5281 gtaataaaaa catttcaatc aattattggg aaaaatatta attatagttt tggccctaac
     5341 cgaagcggcg atgttgcaga atgttgggcg gatgtttcca aagctgaaaa agaactggac
     5401 tggaaggcta ggcgagattt gaccgaaatg ctttcttctg cgtggaaatg gcatacgcta
     5461 aatcctgatg gatacaatta aatataaaag gtgcctatgt tatatttgga tggtattata
     5521 tatagtctcc agtcatacgg tggcatttct gtttattata atcaaataat aaaagcaata
     5581 aaagccacaa atattgaata tacgatatta aactatgaga atacaggctg tcaatttata
     5641 agtgaagatg tgcgacaacc tggacgttat attaatctat atcctcgctt tcttgaaagg
     5701 tatcgagctg ttgatattcc tgaaaactct ctattacatt ctagttatta tagatttaat
     5761 aagggcaaaa aaaataaaaa cataacaaca gtacatgact ttacatatga aaaatatatg
     5821 tcaggtttac gtaaatacgt tcattgtcat cagaagtaca atgctataaa aaacagtgat
     5881 aaaataattt gcatttcaga aaatactgcc aatgatttgt tgaggtattg tccagtacct
     5941 gaaagtaaaa tcgaaatcat tcacaatggg gtgtctgata atttttatga attaaaaaac
     6001 gttaattata acgatgatga taatttcatg ctttttattg gttcgagaaa agcatataaa
     6061 aactttcaac ttgcagttga agcaataaac aaattagagg ggttcaaact atatattgta
     6121 ggtggaggcc ctttgggaaa ggctgagaaa tttttacttg ataaatatat accatatcga
     6181 tatattcatt atccatattt aactgagcaa gagttgaatc taaaatttaa tcaagctcat
     6241 tctcttattt atccgtcaag ttatgaagga tttggtattc ctcttttaga agcaatgagg
     6301 gcaggatgcc cattcattgc gtataatcac tcctcaatac cagaggttgc aggtgacgcc
     6361 ggttatttga tggatgagtt aagtataagc aaccttattg atggtatatc atttataaat
     6421 agaaatcgaa atcaaattat taagaaaggt ttatctcaag cacaacgttt ttcttggaaa
     6481 aaatgtactg acaaaactat cgatatttac aagaaactac tgtaggaata attttatgac
     6541 aaagaaagca ttaattactg gcattactgg tcaagatggt tcatatttgg cagagtttct
     6601 actaaataaa ggttacgagg ttcatggaat aaaacgacgt tcttctattt ttaatactgg
     6661 acgtattgac catatatacc aagatcccca taatattaat ttaaaattac accttcatta
     6721 tggtgactta acagatactt ctaatttgac tcgtatatta tctgaagttc aacctgatga
     6781 agtttacaac ctcggagcaa tgagtcacgt agctgtttcc ttcgaatccc cagaatatac
     6841 agctgatgtt gatgctatag gtactttacg attattggaa gctattcgtt ttttgggatt
     6901 agaaaaaaaa acccgatttt atcaggcgtc aacctctgaa ttgtatggat tagttcagga
     6961 agttccacaa aaagagacaa cgccgttcta tcctcgttca ccttatgccg tagctaaact
     7021 gtatgcatat tggatcactg taaactatcg tgagtcttat ggaatgtatg catgcaacgg
     7081 tattttgttc aatcatgaat ccccacgtcg tggggaaacg tttgtaacac gtaaaatcac
     7141 acgtgctata gctaatattt cgcagggaat agaaaaatgc ttatatcttg gcaacatgga
     7201 ttctttacgt gattgggggc atgcgaaaga ttatgttcgt atgcaatgga tgatgcttca
     7261 acaagaccag ccagaagact ttgttattgc caccggcaaa caaatatctg tacgtgaatt
     7321 tgtccgtatg tctgctaaag aagttggact tgaacttgag ttttccggta ctggtgttga
     7381 tgagattgca atggttgtaa ataaaacatc agattgcgcc gttggtgtta atattggtga
     7441 tgttattgtt cgcgttgacc ctcgttattt tcgaccagca gaagttgaaa ctcttcttgg
     7501 tgatccaaca aaagcaaaaa atgtcttggg ttgggagcca gagattacag ttgaagaaat
     7561 gtgtgcagaa atggtggcta gtgatttaga aaaagctaaa cagcatgcac ttcttaaaag
     7621 ccatggtttt gacgtggcag tctctttgga gcgttaaagt atgaccaaga agcgaatttt
     7681 tgtggctgga catcgtggga tggttggttc tgctatttgt cgtcaattat tagcacgtaa
     7741 tgatgttgag ttaattgtca aaactcacaa agaactcgat ttgacagttc aaaaggatgt
     7801 tgaatgtttt tttgagcaag agagaataga tcaggtttac cttgcagcag caaaagtagg
     7861 aggaattcat gctaataaca cttttccagc tgaatttatt tatcagaacc ttctcattga
     7921 aagcaatatt attcactcat cttataaagc gggcataaag aaactattat tcttagggtc
     7981 aagttgtatc tatccaaagt ttgcagaaca gccaatgaaa gaatctgcac ttttgacggg
     8041 aactcttgag cctactaatg agccttatgc tatttccaaa atagcaggta taaagctatg
     8101 tgaatcttat aatcgccaat atggatgtga ttatcgcagt gtaatgccta ctaacttata
     8161 tggtatgaat gataattttc atcccaataa ctcacatgta attccagcgc ttatgcggcg
     8221 ttttcatgaa gcaaaggaac ttggtttaaa tgaagtggtt gtctggggaa cgggaactcc
     8281 aaaacgcgag tttttatatg ttgatgatat ggcagctgca tcagtatatg tgatggagct
     8341 tgatgatgaa atttataaaa aaaatactca accaatgtta tctcatatta acgtaggtac
     8401 tggtgttgat tgttccatac gtgaaatggc agaaacaatg gctttagttg ttggctatga
     8461 tggaaaaatt gtttttgata taacaaaacc agatggttcg cctcgtaaac ttatggatgt
     8521 tacacgcctt gagaatttgg gctggaaata tcgttataat ttaaaacaag gactagaatt
     8581 aacatataaa tggtttataa ataatcttga ctcatttagg agttaatatt gaacaagaga
     8641 ttagaaacag agttatttaa atcaatagtt gaacatactc cactaatctc gatagacctt
     8701 attataagaa acgaagatgg taaggcgctg cttggtcagc gccttaatcg tccagcacaa
     8761 aattattggt ttgtgccggg gggacggatt cttaaagatg agtctttcga gaacgcattc
     8821 aaaagagtta cttttgaaga attgggtgtt caaattagca ttaatgaagc aaaatttctt
     8881 gggatttatg aacatttcta cagcgacaat ttttctggga cttgtttttc aactcattat
     8941 gtcgtacacg gatatgagat tagtttgatg ccacatcaaa ttaactatcc aacactacaa
     9001 catagtactt acaattggtt cgatatagcc gaattgttag ctaattcctc agttcatcag
     9061 tacacaaaaa attattttaa gtgaaggtta tttatatgct acttcctgtt gtaatggccg
     9121 gtggttctgg aactagattg tggccacttt ctcgaacact atatccgaag caattcctgt
     9181 cgttaactag tcgtttgact atgctacaag agaccttaag aaggcttgaa ggggttgaac
     9241 atcgtcctgc tctggttatt tgtaatgaag ttcatcgttt tatcgttgca gaacagatgc
     9301 gtaatgtaaa tttagcgaat agtggcattt tactagagcc gataggtcgt aatactgccc
     9361 ccgcagttgc gcttgctgct cttaaagcga tttcatcagg tgaggatcca atactgcttg
     9421 tactagcagc cgatcatgaa atacaagacc aaaagcgctt tatatcgtca atattggctg
     9481 caaaagaatt tgcagaggaa ggtaagctgg taacttttgg tattgtgcca accaaaccag
     9541 aaactggata tggctatata aaaaccggag aaaatttaaa tgaatatggt tttaaagttt
     9601 ctgcttttgt tgaaaagcct gagctagatg tggccaaaaa atatcttgaa gatggagatt
     9661 atctttggaa tagtggaata ttcatgttta gggcttctgt gtttattgat gagttgaata
     9721 aatttagacc agacatacta aaaatatgcc aacaagcatt gaagttctct acacaagatc
     9781 ttgattttat ccgtatagat aatgactcat ttagttgttg ccctgaagaa tccattgatt
     9841 acgctgttat ggagaaaaca acagaggctg tggtagttcc attaaatgcc cactggagcg
     9901 atgttggttc ttggtctgca ttatgggaaa ttagcagaaa agataaaagt ggaaatgcaa
     9961 ttagaggaga tgtattaatt catgattctt ctgacagtta tctttactca caacatagat
    10021 taattggtgt agtaggagtt aaagatttag tagttgttga aactaaagat gctattctcg
    10081 ttgcgcataa agataaagtt caacaggtaa aaaatattgt agaaaaactt aaagttaata
    10141 accgtacgga atatctgcag catcgtgaaa tatttaggcc atggggcagt catgactcta
    10201 tagcggaagg atctcgattt gaggttaaac atgtcgttat tcatcctgga cataaaactg
    10261 ctaaacagat tcattaccat cgtactgagc attggattgt tgtttctgga acagcaaaag
    10321 tccattatga agatgaaata tttcttgttt ctgaaaatga atcaacttat ataccaatag
    10381 gtgtacctca ttttattgag aatccaggaa agatcccttt ggagattatt gaggtacgtt
    10441 caggtgttta cttggatgaa gatgatgttg ttagaatcta aaatgatgct ggatactaat
    10501 gcaacattat tattcgtaat gcggtactga cgttatttaa ttatgattat tattggatgt
    10561 taaaatgaaa gtcagtatta ttacagtgac ttataatagt gaaaagactt taagggatac
    10621 tttagagtca atagagttgc aaacctataa agatatagag tatataatta ttgatggtgg
    10681 ttctacagat aatacgttaa aactaattaa cgaagtatca acaagagtaa ccaaatgtct
    10741 atcagaaagc gataacggta tctatgatgc tttaaataaa ggtataaatt tatctacggg
    10801 agatattatt ggattcgttc attctgatga ccttttagca agacctgata ttattgaaac
    10861 tattgtaagt cgtttccatg agacaaaagc ggatgttgta tacggtgatt tggttttttt
    10921 cgaaaaaaac caaattgata taatcaaacg atactggcgt agtggtcctt ttaaacgttc
    10981 taagctatca ttaggctggg caccaccgca tccatctttc tatatgagga gggaattata
    11041 taaagacgat ggatattttg atttatccta taggattgct gcagactatg atcagatggt
    11101 caggattttg aaacgtgatg atattaaagt tatttatgtt cctcaagtat ttgtaaagat
    11161 gagattgggt ggggaaagta ccagaattga taacgcgata tcaagtacaa aggaaattgt
    11221 agaagttatg aaaaaccata atgtaaattg gaaaattgct atcattatca gaaaaatctc
    11281 aaaactaatg caattatttg cgcataaata attttattat tggtaaggtt tttatatgaa
    11341 aaaattaacc tgctttaaag cctatgatat tcgtgggaaa ttaggcgaag aactgaatga
    11401 agatattgcg tggcgcattg gtcgcgccta tggcgaattt ctcaaaccga aaaccattgt
    11461 gttaggcggt gatgcccgcc tcaccagcga aaccttaaaa ctggcgctgg cgaaaggttt
    11521 acaggatgcg ggcgtcgacg tgctggatat tggtatgtcc ggcaccgaag aaatctattt
    11581 cgccacgttc catctcggcg tggatggcgg cattgaagtt accgccagcc ataacccgat
    11641 ggattacaac ggcatgaagc tggtgcgcga aggggcccgc ccgatcagcg gcgataccgg
    11701 actgcgcgat gtccagcgtc tggcagaagc caacgacttt cctcccgttg atgaaaccaa
    11761 acgcggtcgc tatcagcaaa tcaatctgcg tgacgcttac gttgatcacc tgttcggtta
    11821 tatcaacgtc aaaaacctca cgccgctcaa gctggtgatt aattccggga acggcgcggc
    11881 gggtccggtg gtggacgcca tcgaagcccg ctttaaagcc ctcggcgcac cggtggaatt
    11941 aatcaaagtg cacaacacgc cggacggcaa tttccccaac ggtattccta acccgctact
    12001 gccggaatgt cgcgacgaca ctcgcaatgc ggtcattaaa cacggcgcgg atatgggcat
    12061 tgcttttgat ggcgattttg accgctgttt cctgtttgac gaaaaagggc agtttatcga
    12121 gggctactac attgtcggtt tgctggcaga agcgttcctc gaaaaaaatc ccggcgcgaa
    12181 gatcatccac gatccacgtc tctcttggaa taccgttgat gtggtgaccg ccgcgggcgg
    12241 cactccggtg atgtcgaaaa ccggacacgc ctttattaaa gaacgtatgc gcaaggaaga
    12301 cgccatctat ggtggcgaaa tgagcgctca ccattacttc cgtgatttcg cttactgcga
    12361 cagcggcatg atcccgtggc tgctggtagc cgaactggtg tgcctgaaag gaaaaacgct
    12421 gggcgaactg gtgcgcgacc ggatggcagc gtttccggca agcggtgaga tcaacagcaa
    12481 actggcgcaa cccgttgagg cgattaaccg cgtcgaacag cactttagcc gcgaggcgct
    12541 ggcggtggat cgtaccgatg gcatcagcat gacctttgcc gactggcgct ttaacctgcg
    12601 ctcctccaac accgaaccgg tggtgcgcct gaatgtggaa tcgcgcggtg atgtgccgct
    12661 gatggaagaa aagacaaaac ttatccttga gttactgaat aagtaattga gtaaattcat
    12721 ataaataggt tttaaaagac ggaaaagatg agatattcag tgtggtatcg caaaggtaat
    12781 gctattcacc atctctatga gtgagttaac atctatacca catttaagcc gcacactcgg
    12841 cggtgaccac ccctgacagg agtaaacaat gtcaaagcaa cagatcggcg tcgtcggtat
    12901 ggcagtgatg g
//

</pre>