LOCUS       DQ676934               15783 bp    DNA     linear   BCT 06-FEB-2007
DEFINITION  Escherichia coli strain CB9827 serogroup O123 O antigen gene
            cluster, complete sequence.
ACCESSION   DQ676934
VERSION     DQ676934.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15783)
  AUTHORS   Beutin,L., Wang,Q., Naumann,D., Han,W., Krause,G., Leomil,L.,
            Wang,L. and Feng,L.
  TITLE     Relationship between O-antigen subtypes, bacterial surface
            structures and O-antigen gene clusters in Escherichia coli O123
            strains carrying genes for Shiga toxins and intimin
  JOURNAL   J. Med. Microbiol. 56 (PT 2), 177-184 (2007)
   PUBMED   17244797
REFERENCE   2  (bases 1 to 15783)
  AUTHORS   Beutin,L., Wang,Q., Naumann,D., Han,W., Krause,G., Leomil,L.,
            Wang,L. and Feng,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2006) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, TEDA, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..15783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CB9827"
                     /serotype="2"
                     /db_xref="taxon:562"
                     /note="serogroup: O123"
     misc_feature    108..15593
                     /note="O antigen gene cluster"
     gene            108..1181
                     /gene="rmlB"
     CDS             108..1181
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ABG81797.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNSNEALPLFTEMTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVMTQGVVGETYNIGGRNEKKNLDVVNTICDL
                     LDEIVPKQGSYRDQIIYVTDRPGHDRRYAIDASKISDELGWKPQETFESGIRKTIGWY
                     LNNLEWCRRVQDGSYQRERLGLQ"
     gene            1182..2051
                     /gene="rmlA"
     CDS             1182..2051
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ABG81798.1"
                     /translation="MKGIILAGGSGTRLYPVTMAVSKQLLPVYDKPMIYYPLSTLMLA
                     GIRNILIISTPQDIPRFMGLLGDGSQWGLQLEYKIQNSPDGLAQAFILGEDFIGNDNC
                     ALILGDNIFYGHDLQKHLEIALSKDKGATVFAYHVKDPMRYGVVEFDKQGKAISLEEK
                     PEIPKSNYAVTGLYFYDNNVVEIAKSLKPSKRGELEITDVNRLYLERGELSVAMMGRG
                     YAWLDTGTHESLIEASNFIQTIEARQGLKVSCPEEIAFNKKFIDKTQLIKLAKPLEKN
                     SYGKYLIKLAESN"
     gene            2169..3614
                     /gene="wzx"
     CDS             2169..3614
                     /gene="wzx"
                     /note="O unit flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ABG81799.1"
                     /translation="MNSNVKKNISAVNGLKWSAIERICSQGIQLLLMIVLARQLGPGA
                     FGLIGMLTIFITIGQVFIDSGFSAALIRKNERTESDYATVFYFNMTVAILFYAVLFFC
                     APFIAEFYKRNELIELTRVLGLTIIISAFIIVQRIQLSVILDFKTQAISSLSSVIISG
                     GCALLMAYNGFGVWSLVIQTITMGLVNLVILNIYNPWLPKRSFSKKSFHGFFSFGSRL
                     LISSLIDSIYTNIYLVVIGKSFSASTLGQFTQANLLSNTPAMTLTTVVQRVTYPLLSN
                     VNNAKGNIDEIYLRILRLTAAAVFPVMFLLAIIAKPFVVLFLGQQWEPVAELMSILCI
                     GYCLYPVHAINLNLLQVKGRTDLFLKLEIIKKTLITVILIVTIPFGVKIICIGIFAQY
                     YISLLINTYYTGKLSSLSAIAQIKALLPIWLMASISSAISWFLIPREIFSELYQIIGI
                     LITNISLYGIGMYLFQKDIYEMVKFLFIKTK"
     gene            3618..4748
                     /gene="vioA"
     CDS             3618..4748
                     /gene="vioA"
                     /note="dTDP-viosamine synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="VioA"
                     /protein_id="ABG81800.1"
                     /translation="MNKEMKMLNGKILVTQPFLPELREFIPYLEKIWENKWLTNNGPF
                     HQQLENDLCRYLGVEYVSLFNNATIALITAVQSLELTGEVITTPYSFVATTHSLMWNN
                     LNPVFVDVSRDTFNINPSQIEAAITEKTTAIMAVHCYGNPCDVIAIEKIAKKYKLKVI
                     YDAAHAFGVNFKGESLLKYGDLSVVSFHATKVFNTFEGGVIICPNAETKLKIDQLKNF
                     GFEDELTIKSIGINGKMSEVNAAFGLVQLKHVNEAISKRKEINDLYGKLLGNVKGISL
                     AKFDKLATKNFSYYPILIEDDYGMSRDELCHLLQKNNIFARKYFYPLISDMDLYKNME
                     SARKENLHIARDISNKVLCLPIYADLDLDIVRFIARVIGNKK"
     gene            4745..5452
                     /gene="wfbA"
     CDS             4745..5452
                     /gene="wfbA"
                     /codon_start=1
                     /transl_table=11
                     /product="WfbA"
                     /protein_id="ABG81801.1"
                     /translation="MKLAIMQPYLFPYLGYYQLMSSVDKFIIYDDVSYIKNGWINRNR
                     ILVNGNAHYFTVPVIGGSCNNKINTVKIDKTKKKAINKIIITIEQAYKKSVFFDEVFP
                     VIYGVLSKEYDFISDLAITSLLSIKNKLDIGAEVVLTSTNYGNNNLTSQDRVIDINVK
                     EHASTYINSEGGRLLYDKKTFKLNGVNLKFIHPEILPYKQLCNGEFVPSLSIIDVVMN
                     NGWDTTKQLVNSFELKD"
     gene            5456..6430
                     /gene="wfbB"
     CDS             5456..6430
                     /gene="wfbB"
                     /codon_start=1
                     /transl_table=11
                     /product="WfbB"
                     /protein_id="ABG81802.1"
                     /translation="MREHNYAIGGYFSLELQALKNNTANEKVYLQSARACFQLLLESI
                     EVSRVWLPYYICDVVVDTINEIGIEILYYSISKDFIPQTFPVLEENDVFVYVNYFGVC
                     DEQTKLILQKYPPEKVILDNSQAFYSGHNNNLGTIYSPRKFFGVPDGGILITNQTIIL
                     PSSQDNDSSQYINHLIGRLISHPSEYYTDYIKAEERLKKIKKAKVMSYLTRKLLDSIN
                     YHEIKKIRDDNFKFLHHALEKINGIKIPEIVNGPLCYPLLSKNNKLKDILIKNEIYVP
                     TYWKDVLNRVDINSTEFEFVSNLIPLPCDQRYSSIQMKKIINIVLEEN"
     gene            6430..6996
                     /gene="wfbC"
     CDS             6430..6996
                     /gene="wfbC"
                     /note="acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfbC"
                     /protein_id="ABG81803.1"
                     /translation="MNIIGKTVKLRAVEIDDLELLNKWANDPEIWYMLGGWHFPYSKN
                     NTEKWIKNIDNNDSKNQIFAIETEEHGLIGTANLVNIDWKNKNAFHGIMLGNVETRGK
                     GYAQDVVMSLMRYAFDELGLNRLDGDMIEYNKLSINFYIKRCGWKIEGIKKEWFFRKG
                     QYFDKVVVGITKKEYLEHIEKSKYWETK"
     gene            6993..7394
                     /gene="wfbD"
     CDS             6993..7394
                     /gene="wfbD"
                     /codon_start=1
                     /transl_table=11
                     /product="WfbD"
                     /protein_id="ABG81804.1"
                     /translation="MMERFKLGDTATYTQTITDADIKSFAGISGDNNPVHMSDEYAEG
                     SRFKKRIAHGLISASFFSALFGTKLPGPGCVYVNQSLKFLRPVYINDTVTARVVLTDI
                     DVVKRRLFFDTICEVNRKKVITGKAEIYLPE"
     gene            7468..8493
                     /gene="wfbE"
     CDS             7468..8493
                     /gene="wfbE"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfbE"
                     /protein_id="ABG81805.1"
                     /translation="MNVSMSDIKVSVCIISFNQQNYIRQCLDGVFSQKTNFEYEVIIR
                     DDCSTDNTYLTIMEYIDTLDEEKKKNIKITVLDGTKNIGANNNFIETFKTSVGQWLAI
                     CEGDDYWCDQGKLQKQYDYAISHSDCSLVVHPALISENNVIRKTSWACMNKTINQLSD
                     VIRAKGQFSPTGSYFFKREILNVLPLWFSTAPVGDYYMEIFATSLGSCHTIPDAMSVY
                     RINSTGSWSDLLKKDRNGQRIINTYLSQLEYLDKLAEIFPSCVDDITIKRSHAQYAAA
                     MGYLANGDYTNFRILMDKSDTNGWYDNMHSIFYYLRNSRALSMLMFRFKPAIKSAVMW
                     GRRVFNN"
     gene            8477..9727
                     /gene="wzy"
     CDS             8477..9727
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ABG81806.1"
                     /translation="MYLTTNYCDFLSRTRIVRRIFLLVYLISNVYAYIVFTETGVLIG
                     DYQGVLIIYKDQVVYLLMLTIMSYYIVCGPVFNCLSKVKIRLRTFKGLNSFAYILFFF
                     QILFAVFNISTGSNAAGTDFQTGGVIRLLWLFLPVDYLFYIYYFVGREKKVSKIYLAN
                     VVIFILSMLSRGWLGWTLVLLYAELCFFFYSQKKIKIKYLILLFFLLIVAPLAFSLKI
                     QLRADLYSSGIGGVISTLSNIDYIQSYNNFIAGFLSRIQQLSNIVFFYDHQQELYKFV
                     SSDIVSNYAWEGLPQQTVAKLLGLDPGVDMHIFLYSHYISSTSEAVTTLQVGFISWLF
                     LGTLSSVFYPLFVFAIICISLFLSKKLGGEKLCALTWIMIFLSIMCGWYNAYLVYMQA
                     LITFYFIMGFLNLITLEKTKINHT"
     gene            9724..10845
                     /gene="wfbF"
     CDS             9724..10845
                     /gene="wfbF"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfbF"
                     /protein_id="ABG81807.1"
                     /translation="MMDDEKYMSNLVIVNATALASSGALTILNQFLEHALNDSKHKYL
                     CFIHESVKKKSVNNVTVITIKKQSFIQRVWWDLYGLNKYIKKNNLKPKKIVSLQNTSV
                     NSNFEQIIYLHQPIPFSNFRIKIKFQYAIFFMYKYVYPFFIFFRTKNTTFVVQTDWMK
                     DAIVAKKKIAKERVHVIKPDIILPVNNLAYPDEHDKDNSSEVFFLYPATPLFYKNHLI
                     ILDAMRILKTEGILANTKFQVTFKQDDNDELAIKIAKYDLVDNISFLGVLSYEELFHK
                     YLKADAILFPSYLESFGLPLAEGAMLGKYIICSDLPYARDVLNNYSNVEYINHDDASK
                     WALAMKEIILKNRNNTLSESENSYMYCPKTSWADFFELI"
     gene            10858..11889
                     /gene="fnlA"
     CDS             10858..11889
                     /gene="fnlA"
                     /note="L-QuiNAc synthase"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlA"
                     /protein_id="ABG81808.1"
                     /translation="MFKNKVLLITGGTGSFGNAVLQRFLDSDIGEIRIFSRDEKKQDD
                     MRKKYASDKLKFYIGDVRDYSSVLTATRGVDFIYHAAALKQVPSCEFYPMEAVKTNVI
                     GTDNVLEAAIANKVSRIVCLSTDKAVYPINAMGTSKAMMEKVIVAKSRNLPKDITICA
                     TRYGNVMASRGSVIPLFINQILEGRPITITDPCMTRFMMTLDDAVDLVLHAFEHGTNG
                     DIFVQKAPAATIDTLTKSLLKLTKQHSHPINIIGTRHGEKLFEVLCSREEMLVAEDQG
                     NYYRIPSDKRDLNYEKFFDKGTKEIQFVEDYNSHNTRRLDVDEMVALLRKLDYINKIE
                     AGEKADPDA"
     gene            11882..12751
                     /gene="qnlA"
     CDS             11882..12751
                     /gene="qnlA"
                     /note="L-QuiNAc synthase"
                     /codon_start=1
                     /transl_table=11
                     /product="QnlA"
                     /protein_id="ABG81809.1"
                     /translation="MRKKILIVGANGMLGSSLLRYFSSIGDYEVLGTTRSMVVAKQLE
                     QKHNVKIIDNVDVIDFKRLETVVVEHKPNIVFNCVGIIKQLDAAKNNILSIEINSLLP
                     HKLAQLCSAHSAKLIHFSTDCIFKGTKGNYVEDDESDAIDLYGKSKFLGEVEYNGHLT
                     LRTSIIGHELGSNHSLVDWFLSQKKSVKGFTNAIFSGLPTCYMAEVIHKYVLPNNLAG
                     LFHLSVEPISKYDLLNIIKIVYGVSTDIEPTNEFKIDRSLNSTLFRNKTNFVPESWDK
                     LIEKMKDEYNKYF"
     gene            12729..13883
                     /gene="qnlB"
     CDS             12729..13883
                     /gene="qnlB"
                     /note="L-QuiNAc synthase"
                     /codon_start=1
                     /transl_table=11
                     /product="QnlB"
                     /protein_id="ABG81810.1"
                     /translation="MNTINISNSKIRVVTVVGTRPEIIRLSRVIAVLDEYTEHFLVHT
                     GQNYDYELNEVFFNELEIRKPDFFMNAAGQNAAETIGNVIIEADKIFDKLQPEALLIL
                     GDTNSALVSIAAKRRKIPIFHMEAGNRCFDYRVPEEINRKIVDHISDINLTYSEIARD
                     YLLREGLPADQIIKTGSPMREVLNFYKDKISSSSILEKLNLQASSYFLVSSHREENVD
                     SPEKLRSLIETLNIVSEKYKLPVIVSTHPRTRNRIDALGITVSNNIIFSKPFGFLDYI
                     KLQQNARVVLSDSGTITEESSVLNFPALNLREVHERPEGFEEAVVMFVGLDRNRIIQG
                     IDILQGQKRGDNDRDLHMVTDYQADNVSIKILRIIMSYTNFINQKVWKKF"
     gene            13883..15049
                     /gene="wbwH"
     CDS             13883..15049
                     /gene="wbwH"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbwH"
                     /protein_id="ABG81811.1"
                     /translation="MRIALICDDYLPDSTRVSAKMMHELACELLEKGHEPIVICPCNK
                     IQTLEILNLDGVVVYKFPNGAIKNVSKISRAINESMLSFNAWRFIGKYIRETKIDGVV
                     YYSPSIFFGKLANKIKENWHCKSYLILRDSFPQWLVDQGIIKEGGLAERYFRYFEQIN
                     YDAADYIGLMSDRNKDIFINKYQNKYKVQTLFNWADFKGIDNIPSATLRSKLALQNKV
                     IFFYGGNIGHAQDMMNLMRLVRSASYRDDVHFLLIGQGDEVSLVKQFIIDNSLKNCTY
                     LPSITQSEFKSVLKIVDVGLFSLAKNHTVHNFPGKLLGYMANKLPILGSVNASNDVME
                     IINGAKAGFVFVNGNDEALLNAAINLADDTQLRKNLGCNAYSLLQKKILCGNGC"
     gene            15096..15593
                     /gene="wbuC"
     CDS             15096..15593
                     /gene="wbuC"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC"
                     /protein_id="ABG81812.1"
                     /translation="MKVSFVSQDDFENLIQEAFASVRLRSHLLLHESPNDAVQRIMIG
                     LVKGTYIPPHFHEFQHQWEHFHVFQGEVELMLFDSNGCLNKKVILGGQSKNIIAQISP
                     LTPHTLVCRSPTAVIMEIKEGPFDEKCAKVIPSWSYSEDYSILSRDRIIAMMGQLSIG
                     DRFSL"
ORIGIN      
        1 attggtagct gtaagccaag ggcggtagcg tgcattaata cctctattaa tcaaactaag
       61 agccgctaat ttaacagcat gctctgaagt aatatggaat aaattaagtg aaaatacttg
      121 ttactggtgg cgcaggattt attggttctg ctgtagttcg tcacattata aataatacgc
      181 aggatagtgt tgttaatgtc gataaattaa cgtacgccgg aaacctggaa tcacttgctg
      241 atgtttctga ttctgaacgc tatgtttttg aacatgcgga tatttgcgat gctgctgcaa
      301 tggcgcggat ttttgctcag catcagccgg atgcagtgat gcacctggct gctgaaagcc
      361 atgtggatcg ttctatcact ggccctgcgg catttattga aaccaatatt gttggtactt
      421 atgtcctttt agaagccgct cgcaattact ggtctgctct tgatagcgac aagaaaaata
      481 gtttccgttt tcatcatatt tctactgacg aagtatatgg cgacttaccc catcctgatg
      541 aagtaaatag taatgaagcg ttaccgctat ttacggaaat gacagcttat gcaccaagta
      601 gcccatattc tgcttctaaa gcatccagcg atcatttagt ccgcgcgtgg aaacgtacct
      661 atggtttacc gaccattgtg actaattgtt ctaacaatta tggtccttat catttcccgg
      721 aaaaactgat tccattggtt attcttaatg ctctggaagg taaggcatta cctatttatg
      781 gtaaggggga ccaaattcgc gactggttgt atgtagaaga tcatgcccgg gcattgtaca
      841 ccgttatgac ccagggtgta gtaggtgaaa cctacaatat cggtggtcgt aacgagaaaa
      901 aaaacctgga cgtggtaaat actatttgcg atctgcttga tgagattgta cctaaacaag
      961 ggtcttatcg cgatcaaatt atctacgtta ctgaccgtcc tgggcacgat cgccgctatg
     1021 cgattgatgc atcaaaaatc agcgatgagc tgggctggaa accgcaggaa acctttgaat
     1081 cgggaattcg aaaaactatt ggttggtatt taaataattt agagtggtgc cgtcgtgtgc
     1141 aagacggtag ctatcaacgt gaacgtttag gattacagta aatgaaaggt atcatacttg
     1201 caggtggttc aggaactcgt ttatatccag tcacaatggc tgtaagtaaa caattgttgc
     1261 cagtttatga taaaccaatg atttattacc cattaagtac attaatgttg gctggtatac
     1321 gaaatatact tattataagt acacctcaag atattcctcg ttttatgggg ttattaggtg
     1381 atggtagtca atggggactt cagttagaat acaaaataca gaatagtcct gatggattag
     1441 cgcaagcatt tatattaggt gaggatttta ttggaaacga taattgtgca ttgattttag
     1501 gggataatat attctatgga catgatctcc agaaacacct agaaatagcg ctctcaaaag
     1561 ataaaggtgc tacagttttc gcttatcatg ttaaagaccc tatgagatac ggcgttgtag
     1621 agtttgataa acaaggtaaa gccatttcac ttgaagagaa acctgaaatt ccaaaaagta
     1681 attatgcagt aacgggattg tatttttatg ataataatgt tgttgagata gcgaaatcat
     1741 taaaaccctc gaagcgagga gagctagaaa taacggatgt taatcgactc tatttggagc
     1801 ggggtgagct ttctgtagct atgatggggc gaggatatgc ttggctagat actggaactc
     1861 atgaaagtct aattgaagct agcaatttca tccagacaat tgaagctaga cagggtttaa
     1921 aagtatcctg ccctgaagaa atagcattta ataaaaaatt tattgataaa acgcagttaa
     1981 taaaattagc taagcctttg gagaaaaatt catacggaaa atatttaata aaactggcag
     2041 agtcgaattg attttttatg aatgattaat ttgttcatag aataaaaagt gaatgcctcc
     2101 atggttaatg agttaattta acaactaaga aggtgagtat ctatctctaa gtgttattga
     2161 gaaggtatat gaattcaaat gtaaaaaaaa atattagcgc tgtaaatgga ttgaagtgga
     2221 gtgcgatcga aagaatatgt tcacaaggta tccagctact tttaatgata gtcttggcta
     2281 gacaattagg gcctggtgca tttggcctta ttggaatgct gacaatattt attacaatag
     2341 gtcaggtctt tattgatagt ggttttagtg ccgctctcat tcgaaaaaat gaaagaacag
     2401 aatcagacta tgcaactgtt ttttacttta acatgacagt tgccattctg ttttatgcag
     2461 tgttattttt ttgcgcccca ttcatagctg aattttataa gcgtaacgaa ttaattgaat
     2521 taacaagagt tctgggttta acaataataa taagtgcttt tattattgtt caacgaatac
     2581 aattaagtgt cattttggat ttcaaaactc aagctatatc gtcattatcc agtgtcataa
     2641 tctcaggagg gtgtgcacta ttaatggcat ataatggttt tggtgtatgg tcgttagtta
     2701 tacagactat taccatgggg cttgttaatt tagttatttt aaatatatat aatccatggt
     2761 taccgaagag gagtttttca aaaaaatcat ttcatggatt tttttctttt ggctccagac
     2821 ttctgatttc atcactgata gattcaatat acactaatat ttatttggta gttataggga
     2881 agtcctttag cgctagcaca ctgggccaat ttacacaagc taatttatta tcaaatacgc
     2941 cggccatgac gttaacgaca gttgtacaaa gagttaccta tccattatta agtaatgtga
     3001 ataatgctaa ggggaatatt gacgagatat atcttaggat attaaggctt actgccgcag
     3061 ccgtttttcc agtaatgttc ctattggcaa taattgctaa accttttgtc gttctatttc
     3121 ttggccaaca atgggaacct gttgcggaat taatgagcat attatgtata ggatactgtt
     3181 tatatccagt acatgctatt aatctaaact tattacaggt aaaaggacga actgatttat
     3241 ttttgaagtt agaaataata aaaaaaactc tcatcacggt tattctaata gtaacaatac
     3301 catttggtgt taaaataata tgtataggta tttttgcaca gtattatata tccttgttga
     3361 taaatacata ctatacaggg aaactcagca gcttaagtgc aattgcacag ataaaggcat
     3421 tattgccaat ttggttaatg gcatctatca gttcggcaat tagttggttc ttaataccaa
     3481 gagagatatt ttcggaatta tatcaaataa taggaatatt gataaccaat atttcattat
     3541 atggaatagg aatgtatctt ttccaaaaag atatttatga aatggtaaag tttttattta
     3601 taaaaacaaa ataatttatg aacaaagaga tgaaaatgtt aaatggcaag attttagtaa
     3661 cgcaaccatt tttacctgaa ctaagagagt ttattcccta tctggaaaaa atatgggaaa
     3721 ataaatggtt aacaaacaat ggtccatttc atcagcaatt agaaaatgat ttgtgccgtt
     3781 atttaggcgt ggagtacgtc tccttattta ataatgctac aattgctctg attacggcag
     3841 tccaatcatt agaattgact ggtgaagtaa ttacaacccc gtattcattt gtggcaacaa
     3901 ctcactcatt gatgtggaat aatctaaatc cagtatttgt cgatgtcagt agagatacat
     3961 ttaatatcaa tccgtctcaa attgaagcgg cgataacaga aaaaacaaca gccataatgg
     4021 cagtccattg ttatggtaat ccttgtgatg tgattgcaat agaaaaaata gccaaaaagt
     4081 ataaacttaa agtgatatat gatgctgctc atgcttttgg ggtaaatttt aagggggaaa
     4141 gtttattaaa atacggagat ttatcggttg ttagttttca tgcaactaaa gtatttaata
     4201 catttgaagg tggcgtgatt atttgcccaa atgcagaaac taagctcaaa atagatcagc
     4261 taaaaaactt tggttttgaa gatgagctaa caataaaatc aattggtatt aatggaaaaa
     4321 tgagtgaggt caatgcagca tttggccttg tacagttgaa acatgttaat gaagctataa
     4381 gtaaaagaaa agaaattaat gatttatatg gcaagttatt agggaatgtg aaaggcatat
     4441 cattagcaaa atttgataaa ctagctacga aaaatttttc atactacccg attcttattg
     4501 aagatgacta tgggatgagt cgagatgaat tatgtcattt acttcaaaaa aataatatat
     4561 ttgctaggaa atatttttat cctttaataa gcgatatgga tttatataaa aatatggagt
     4621 cagcgagaaa agaaaatcta cacattgctc gagatatttc caacaaagta ctttgcttac
     4681 caatttatgc agatcttgat ttagacattg tgagatttat agcgagagta ataggtaata
     4741 aaaaatgaaa ttagcaataa tgcaacccta tctatttcct tatctaggtt attatcaatt
     4801 aatgtcatca gttgataagt ttattattta tgatgatgtt tcatacatca aaaatggttg
     4861 gataaacaga aatagaattc ttgttaatgg taatgctcat tattttactg tgccagttat
     4921 aggtgggagt tgtaataata aaataaatac tgttaaaatt gacaagacaa agaaaaaagc
     4981 tatcaataaa atcattatta caattgaaca agcctataaa aaatccgttt tttttgatga
     5041 ggtctttcca gtcatttatg gtgtgttatc taaagagtat gatttcatat ccgatctggc
     5101 aataacgtca ttattgtcaa taaaaaacaa acttgatatt ggagcagaag ttgttttgac
     5161 ttcgaccaac tatggtaaca ataatttaac ttcgcaagat cgcgtcattg atattaatgt
     5221 aaaagagcat gcgtccactt atattaattc tgaaggcggg agattacttt atgataaaaa
     5281 gacattcaaa ctaaatggcg taaatttgaa atttatacat ccagaaattt taccttacaa
     5341 acaactttgt aatggtgagt ttgtaccatc cttatcaatt atagatgtag taatgaataa
     5401 tggttgggat actacaaagc agttagtaaa tagctttgaa ctgaaggatt gaacaatgcg
     5461 tgagcataat tatgcgatcg gaggatattt ttcactagaa ttgcaggcgc ttaaaaataa
     5521 cactgcaaat gaaaaagtat atttacaatc agcgagggca tgttttcaat tactgctaga
     5581 aagcattgaa gttagtagag tatggttacc atattatata tgtgatgtcg tcgttgatac
     5641 aataaatgaa attgggattg aaatcttgta ttatagtatt tctaaagatt ttattccgca
     5701 gacgtttcca gttttagaag aaaatgatgt ttttgtctat gttaattatt ttggggtgtg
     5761 tgatgagcaa acaaagttaa ttttgcaaaa atatcctcca gagaaagtta tcttagataa
     5821 ctcacaagct ttttatagcg gacacaataa taatttagga acaatttatt cacctaggaa
     5881 attttttggc gtccctgatg gtggtattct gataactaat cagactataa tattaccttc
     5941 ttcacaggat aatgattcat cacagtatat aaatcatctc attgggcgtc taatatccca
     6001 tcccagcgaa tactatactg attatattaa agctgaagaa cgattaaaaa aaattaaaaa
     6061 agccaaagtg atgtcttatt taactcggaa attattagac tccatcaatt atcatgagat
     6121 taaaaaaata agggatgata attttaaatt ccttcaccat gccctcgaaa agataaatgg
     6181 aataaaaatt ccggagatag tcaatggtcc attatgctat ccattgcttt caaaaaacaa
     6241 caaactgaaa gatattttaa tcaagaatga aatttatgtg cctacatatt ggaaagatgt
     6301 attgaatcga gtagatataa actcgacaga atttgaattt gtctcaaact taatacctct
     6361 gccatgtgat caacgttact catctatcca gatgaagaaa ataatcaata ttgtacttga
     6421 ggaaaactaa tgaatatcat aggaaaaaca gttaagttgc gtgctgtgga aattgatgat
     6481 ttagaattgt taaataaatg ggctaacgat ccggaaattt ggtatatgct tggtggttgg
     6541 catttcccct attccaaaaa taatactgaa aaatggataa aaaatattga taataatgat
     6601 tcgaaaaatc aaatattcgc tattgaaact gaagagcatg gattgattgg cactgctaac
     6661 ttagtaaata ttgattggaa aaataaaaat gcatttcatg ggataatgtt gggaaatgtt
     6721 gaaacacgag gtaaagggta cgcgcaagac gttgtaatga gtctgatgag atatgccttt
     6781 gatgaattag gattaaatcg acttgatggt gatatgattg aatataataa attatcaatt
     6841 aatttttaca tcaaaagatg tggttggaaa atcgagggaa ttaagaaaga gtggttcttt
     6901 agaaaggggc aatattttga taaagtagtt gttggaataa ccaaaaaaga atatttagaa
     6961 catattgaga aaagcaagta ttgggagaca aaatgatgga acgttttaaa ttgggggaca
     7021 cagcaactta tactcaaact ataactgatg cagatattaa aagcttcgca ggtatatctg
     7081 gggataataa tccagtacat atgagtgacg aatatgcaga aggttcaagg tttaaaaagc
     7141 gtatagctca tggtctgata tctgctagtt ttttttcagc tctatttggt acaaaattac
     7201 ctgggcctgg atgtgtatat gtaaatcaaa gcttgaaatt tcttagacct gtttatatta
     7261 atgatacggt tacagcgcgt gtagtcttaa cagacattga tgttgtaaaa agaagactct
     7321 tttttgatac gatttgtgaa gttaaccgaa aaaaagttat cacggggaag gctgaaattt
     7381 acttgccaga ataaagcgct ttaaatttga tgttaacttg ctacatattg atgttttatc
     7441 tctttttatc tttgaagttt atcaaaaatg aatgtttcca tgtcagatat aaaagttagt
     7501 gtatgtatta tttcttttaa ccaacaaaac tatatacgac agtgcttgga tggtgtcttt
     7561 tctcagaaaa caaattttga gtatgaggtt attatacgtg atgattgcag taccgataat
     7621 acatatttaa caataatgga atatattgac actttagatg aagagaaaaa aaagaacata
     7681 aaaataacgg tacttgatgg cacgaaaaat attggagcaa ataataattt tatcgaaaca
     7741 tttaagactt cagtaggtca atggctagct atatgtgagg gagatgatta ttggtgtgat
     7801 caggggaaat tacaaaaaca gtacgactac gctatttcac atagtgattg ctcattagtc
     7861 gtgcatcccg ctctgataag tgaaaataat gtaatacgta aaacatcttg ggcgtgtatg
     7921 aataaaacaa taaatcagct tagcgatgta atacgtgcaa aaggacagtt ctcaccaact
     7981 ggctcctatt ttttcaaacg agaaatctta aatgttctac cattgtggtt ttcaacagct
     8041 cctgttggtg actattacat ggaaatcttt gctacatcgc ttggatcttg tcatacaatt
     8101 cctgatgcta tgtcagttta tagaatcaat tcaacggggt cttggtctga cctgttaaaa
     8161 aaagatagaa atggccaacg gattattaat acttatcttt ctcaattgga gtatttggat
     8221 aaacttgccg aaatctttcc ttcttgtgtc gatgatatca caataaaaag atcgcatgca
     8281 caatatgcag cagcaatggg ttatcttgca aatggtgatt atactaattt tcgtatatta
     8341 atggataagt cagatacaaa cggctggtat gataatatgc attctatttt ttattattta
     8401 agaaatagca gggcgctttc catgttaatg ttcagattca agcctgcaat taaatctgct
     8461 gtcatgtggg ggcgtcgtgt atttaacaac taattattgc gactttttgt cccgaactcg
     8521 aatcgttagg cgcatatttt tgttagttta tttgatttca aatgtttacg cctatatagt
     8581 gtttactgaa acaggagtat taatagggga ttatcaagga gtactcataa tatataaaga
     8641 tcaggttgtg tatttattga tgttaacaat aatgtcatat tatattgttt gtggtcctgt
     8701 ttttaactgt ctatcaaaag taaaaattag actaaggaca tttaaaggct taaactcttt
     8761 tgcttatatc ctgttttttt ttcaaatcct atttgctgtt tttaatattt caacaggttc
     8821 gaatgccgcg ggcactgatt ttcaaactgg tggagtcata agactattat ggcttttttt
     8881 accagttgac tatctatttt atatatatta tttcgtaggc cgagagaaaa aagtaagtaa
     8941 aatatatctg gccaatgtgg ttatttttat tctatcaatg ttaagtagag gatggcttgg
     9001 gtggactttg gttttgcttt atgcagagtt atgtttcttt ttttattcac aaaaaaaaat
     9061 taaaataaaa tatcttatct tattgttttt cttgcttatt gttgcaccat tagcatttag
     9121 tctaaaaatt caattgcgtg ctgatttgta ttccagtggt attggtgggg ttatatcaac
     9181 tttaagtaat attgattaca ttcaatctta taataatttc atcgctggtt tcttatccag
     9241 aatacaacag ttatcaaata ttgttttctt ttatgaccat caacaagaat tatataaatt
     9301 tgtttcttca gatattgtat caaactatgc ttgggaagga ttacctcaac aaactgtagc
     9361 taaacttcta ggattagatc ctggtgttga tatgcatatt tttctttata gtcattatat
     9421 ttcttcaact tcagaggctg taactacatt gcaagttgga ttcatttctt ggctcttttt
     9481 aggcaccctg tcatccgtat tttatccact gtttgtcttt gctattatat gcatatcctt
     9541 atttttatct aaaaaacttg gaggggaaaa actatgtgca cttacgtgga ttatgatttt
     9601 tctttctatt atgtgcgggt ggtataatgc ttatttggta tatatgcagg cattaattac
     9661 attttatttt atcatgggtt ttttgaattt aattactcta gaaaaaacaa aaattaatca
     9721 tacatgatgg atgacgagaa atatatgagt aatttagtaa tagttaacgc aacagcttta
     9781 gcgtctagcg gcgctttgac gattttgaac cagtttttag aacacgcact aaatgattct
     9841 aagcataagt atctttgttt tatacatgag agtgttaaaa aaaagagtgt taataatgtc
     9901 accgttatta ctataaaaaa acaaagtttt attcaacgag tatggtggga tctatatgga
     9961 ttgaataaat atataaagaa aaataatttg aaacctaaaa aaatagtttc cttgcagaat
    10021 acatctgtaa atagtaattt cgagcagata atatatctac accagccgat tccctttagc
    10081 aattttagaa taaaaattaa atttcaatat gctatatttt tcatgtataa atacgtgtat
    10141 ccttttttta tattctttag aactaaaaac accactttcg ttgttcagac cgattggatg
    10201 aaagatgcta tcgtagcaaa gaaaaaaatt gccaaagaac gagttcatgt aattaaacct
    10261 gatataatat taccggttaa taatcttgct taccctgatg aacatgacaa agataattca
    10321 tctgaagtgt tttttttata tccggccacg ccacttttct ataaaaacca tttgattatc
    10381 ttagatgcaa tgaggattct aaaaacagaa ggaatacttg ctaatacaaa attccaagtg
    10441 acttttaaac aagatgataa tgatgagtta gcaattaaaa tagccaaata tgatttagtt
    10501 gataatataa gcttcttggg agtattatcc tatgaagaat tgtttcataa atacttaaaa
    10561 gcagatgcta tactttttcc tagctacttg gaaagctttg gtttaccgtt agcagagggc
    10621 gcaatgcttg ggaaatatat aatttgtagc gatttgccat atgcaagaga tgtgcttaac
    10681 aattattcaa atgtagaata tataaatcat gatgatgcca gtaaatgggc attagctatg
    10741 aaggaaataa tattaaagaa tagaaataat actttatctg aaagtgaaaa tagctatatg
    10801 tattgtccga aaacaagctg ggcagatttc ttcgagttaa tctaattgag gtatacgatg
    10861 tttaaaaata aggtacttct cattactggt ggcacaggtt cctttgggaa tgccgtttta
    10921 caacgctttt tagattcaga tattggcgaa attcgaatct ttagtcgtga tgagaaaaaa
    10981 caagatgata tgcgtaaaaa atacgcaagt gataagttaa aattttatat aggggatgtg
    11041 agggactata gcagtgtttt aacagcgact cgtggggttg attttattta tcatgcagca
    11101 gcattaaagc aagtaccttc atgtgagttt tatccaatgg aagctgtcaa gactaacgtt
    11161 attggaactg ataacgtact tgaagctgca attgcaaaca aagtttcacg tattgtttgt
    11221 ttgagtaccg ataaagctgt ttatccaatt aatgctatgg gtacatctaa ggctatgatg
    11281 gaaaaagtta tcgtagctaa gtcaagaaac ttacctaaag atattactat ttgtgcgacg
    11341 cgatatggaa atgtaatggc ttcccgtggt tcggtcatcc ctctgtttat taatcaaata
    11401 cttgaaggac gcccaatcac tatcaccgat ccgtgtatga ctcgattcat gatgactttg
    11461 gatgatgctg tagatcttgt attacatgct tttgaacatg gtactaatgg tgatattttt
    11521 gttcaaaagg caccagctgc aacaatagat acacttacca agtcattact taagttaact
    11581 aaacaacatt ctcacccaat taatatcatt ggcactcgcc atggtgaaaa actatttgag
    11641 gtactctgca gtcgggaaga aatgttggtt gcagaagatc aaggtaatta ctatcgtata
    11701 cctagtgata aacgtgatct aaattatgaa aaattcttcg acaaagggac taaagaaatt
    11761 cagtttgttg aagactataa ttctcataat acccgtcgtt tagatgttga tgaaatggtt
    11821 gctcttctta gaaaattaga ttacatcaat aaaattgagg ctggtgaaaa ggcagatccc
    11881 gatgcgtaaa aaaattctaa ttgttggcgc aaatggcatg ttaggtagta gtttactacg
    11941 ctacttttca tcaattggag attatgaagt tcttggtacg acaagaagta tggtcgttgc
    12001 aaaacaactt gagcaaaagc acaatgtgaa aattattgac aacgttgatg ttattgattt
    12061 taaacgatta gagactgtag tagtagagca taagccaaat attgttttca actgcgttgg
    12121 gataattaaa caacttgatg cagcaaaaaa caatatatta tctattgaaa ttaactcatt
    12181 acttccacat aaattagctc aattatgttc agctcatagt gctaaactca tacatttttc
    12241 aacagattgc atttttaaag gtaccaaagg taattatgtt gaggatgatg agtctgatgc
    12301 aattgattta tatggtaaat ctaagttctt gggtgaagtt gaatataatg ggcatttaac
    12361 tttgcggact tctattattg gccatgagtt gggatcaaat catagtcttg ttgactggtt
    12421 tttatcacag aaaaaatcgg tgaaaggatt tactaatgcg atattctcag gcctcccaac
    12481 ttgttatatg gcagaggtta tccataaata tgttcttccc aacaatcttg ctggtttatt
    12541 tcatttaagt gtagagccaa ttagtaaata tgatttgtta aatattatta aaatagtata
    12601 tggagtaagt actgatatag agccaaccaa cgaatttaaa atagaccgca gcttgaattc
    12661 tacgctattt cgtaataaga caaattttgt cccagaatct tgggataaat taattgaaaa
    12721 gatgaaagat gaatacaata aatatttcta attcaaaaat acgggttgtt actgtagttg
    12781 gcactaggcc tgaaatcatt cgtttatcga gggtaattgc tgttctcgat gagtacactg
    12841 aacattttct tgttcatacc ggacaaaact atgattacga gcttaatgag gtattcttta
    12901 atgagcttga aatcagaaaa cctgattttt tcatgaatgc cgcgggccag aatgctgctg
    12961 agaccatcgg aaatgtaatt attgaagctg ataagatatt tgataaattg caacctgaag
    13021 ctttacttat tcttggtgat acaaatagtg ctctagtttc cattgcagct aaaagacgaa
    13081 aaattcctat ctttcatatg gaagcgggca accgatgctt tgattacaga gttccggagg
    13141 aaataaatag gaagattgtt gatcatattt cagatattaa tttaacatat agtgagatag
    13201 ctagggatta tcttttgagg gaagggcttc ctgctgatca aataatcaaa accggtagcc
    13261 ctatgcgaga ggttctaaat ttttataagg ataaaatttc atcttcttct attcttgaaa
    13321 aacttaacct tcaagcatct agttatttcc ttgttagtag tcatcgagaa gagaatgttg
    13381 attcgcctga gaagttgcgc tctcttattg agacgttaaa tatagtatct gagaaatata
    13441 aactgccagt aatagtatca acgcatcctc gtactcgtaa tagaattgat gctttaggta
    13501 tcacggtaag taataatatc attttttcta agccattcgg atttttagat tatataaaac
    13561 tgcagcaaaa tgctcgtgtc gtgctctctg atagtggaac tataactgaa gaatcatcag
    13621 ttttgaattt tccagcctta aacctgcgag aagtacatga gagaccagaa ggatttgaag
    13681 aagctgttgt tatgtttgtc gggctagata gaaacagaat tattcaggga atcgatattt
    13741 tgcagggtca aaaacgtggt gataatgatc gtgaccttca tatggttaca gattatcagg
    13801 cagacaatgt ttcaattaaa attcttagaa ttattatgag ctatacaaac tttattaatc
    13861 aaaaggtttg gaaaaaattc taatgcgtat tgcgttaata tgtgatgact atttgcctga
    13921 tagtactcgt gttagtgcta agatgatgca tgaattggct tgtgaacttt tagaaaaagg
    13981 acatgagcca atcgttattt gtccttgcaa taaaatacag acgctcgaaa ttttgaattt
    14041 ggatggtgtc gtcgtttata agttccctaa tggtgctata aagaatgtat ctaaaatatc
    14101 aagagcaata aacgaatcca tgctatcatt caacgcatgg cgatttattg gaaaatatat
    14161 tcgagaaact aaaattgatg gtgttgtgta ctactcacca tctatttttt ttggaaaatt
    14221 agcaaataaa atcaaagaaa attggcattg taaatcatat ttaatcttaa gggattcttt
    14281 tcctcagtgg ctggtagatc aggggataat taaggaggga gggcttgcgg aacgatattt
    14341 taggtacttc gagcagatca attatgatgc tgcagattat attggtttaa tgtcggatag
    14401 aaacaaagat attttcatta ataaatatca gaataaatac aaggtgcaaa ctttattcaa
    14461 ttgggctgac tttaaaggta tagataatat tcccagtgcg actctacgat caaaattagc
    14521 acttcaaaac aaagtaattt ttttttatgg aggcaatatt ggtcatgctc aggatatgat
    14581 gaatttaatg aggttggtta gatcagcatc ttatcgtgat gatgtgcatt ttttattgat
    14641 tggacaaggt gatgaagttt cccttgtaaa gcagtttatt atagataatt ctttaaaaaa
    14701 ctgtacgtat ttaccatcca taacacaatc agagtttaag tctgtattaa agattgttga
    14761 tgttggtttg tttagccttg ctaaaaatca tactgttcat aacttccctg gtaaattgtt
    14821 agggtatatg gcaaataaac tccctatatt aggtagtgtt aatgcaagca atgatgttat
    14881 ggagattatc aatggggcaa aggccgggtt tgtttttgtt aacggaaatg acgaggcttt
    14941 acttaatgct gcaataaatc ttgcggatga tactcagcta agaaaaaacc tgggatgtaa
    15001 tgcttactct ttattacaaa aaaaaattct ctgtggaaat ggctgctgaa aagatattaa
    15061 gcagcttatt ttcctaacag taatatggtg gttttatgaa agtgtctttt gtaagtcagg
    15121 atgatttcga gaatttaatt caggaagcat ttgcatccgt tcgcttgaga tctcacttac
    15181 tcctccatga aagccctaat gatgctgtcc agagaataat gataggactt gtcaaaggaa
    15241 cgtatatccc gcctcatttt catgaatttc agcatcaatg ggaacatttc catgtgtttc
    15301 aaggtgaggt tgaattaatg ctatttgata gtaacggctg tttaaataaa aaggttattc
    15361 ttggtgggca gagtaagaat attattgcac aaatttcacc actcacccca catacactag
    15421 tttgtagatc tcctacagca gttatcatgg agattaaaga aggtcctttt gatgaaaaat
    15481 gcgctaaagt catcccctca tggtcatata gtgaagatta ttccatttta tccagagata
    15541 ggatcatcgc aatgatgggc caattatcaa ttggagatag atttagtctc taaacttcac
    15601 agcaattagc gaagtaatct tctttttcat accttctata ttatatttat ccactactat
    15661 tatagctttg aatactgagt taacatctac actacattca agccgtgcat acgtcgcggt
    15721 gaccacccct gacaggagta aataatgtcc aagcaacaga tcggcgtcgt cggtatggca
    15781 gtg
//