<pre>LOCUS       AY208937               15155 bp    DNA     linear   BCT 07-JUL-2003
DEFINITION  Escherichia coli O121 O-antigen gene cluster, complete sequence.
ACCESSION   AY208937
VERSION     AY208937.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15155)
  AUTHORS   Fratamico,P.M., Briggs,C.E., Needle,D., Chen,C.Y. and DebRoy,C.
  TITLE     Sequence of the Escherichia coli O121 O-antigen gene cluster and
            detection of enterohemorrhagic E. coli O121 by PCR amplification of
            the wzx and wzy genes
  JOURNAL   J. Clin. Microbiol. 41 (7), 3379-3383 (2003)
   PUBMED   12843098
REFERENCE   2  (bases 1 to 15155)
  AUTHORS   Fratamico,P.M., Briggs,C.E., Needle,D., Chen,C.-Y. and Debroy,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-DEC-2002) Microbial Food Safety, Eastern Regional
            Research Center, Agricultural Research Service, US Department of
            Agriculture, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA
FEATURES             Location/Qualifiers
     source          1..15155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /serotype="O121"
                     /db_xref="taxon:562"
     gene            153..1433
                     /gene="wbqA"
     CDS             153..1433
                     /gene="wbqA"
                     /note="WbqA"
                     /codon_start=1
                     /transl_table=11
                     /product="putative UDP-glucose-6-dehydrogenase"
                     /protein_id="AAO39690.1"
                     /translation="MSFNLENYKIGIIGLGYVGLPLAVEFGKKIKTLGFDINEKRIKE
                     LSAGNDFTLECSSDELAQAYYLQYSCDYKELATCNVFIVTVPTPIDEYKQPDLTPLVK
                     ASETLGKIIKKDDIIIYESTVYPGATEEVCIPVIEKISGLVFNIDFFAGYSPERINPG
                     DKEHRVTTIKKVTSGSTPEIAEIVDKLYGTIITAGTHKASSIRVAEAAKVIENTQRDL
                     NIALINELAMIFNKLGIDTEEVLKAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQA
                     IGYHPEVILAGRRINDGMSKYVASQFIKAMLKKKFQIEGANVLIMGLTFKENCPDLRN
                     TKVVDILAELKEYNVNVDIYDPWVDVEEAYYEYGINVLNTLPDKKYDGVIFAVAHSQF
                     KNMSKEEVHGIIRNDGVIYDLKYIIDPNLVDIRL"
     gene            1449..2498
                     /gene="wbqB"
     CDS             1449..2498
                     /gene="wbqB"
                     /note="WbqB"
                     /codon_start=1
                     /transl_table=11
                     /product="putative UDP-glucose-4-epimerase"
                     /protein_id="AAO39691.1"
                     /translation="MCQRGCLMKYKKIEDALSSKSETWLITGVAGFIGSNLLEALLKL
                     NQKVVGLDNFATGHQANLDEVKESVSAEQWSAFTFVEGDICEPETCVEVVKGVDHVLH
                     QAALGSVPRSIKDPITTNNTNISGFINMLVAAKDANVRSFTYAASSSTYGDHPALPKI
                     EENIGNPLSPYAVTKYVNELYAQVFARTYGFKSIGLRYFNVFGKRQDPNGAYAAVIPK
                     WTAAMINDEPLYINGDGETSRDFCFIENVVQMNILAAQSDASARDQVYNVAVGDRTTL
                     KQLFTVLKNALNENGVSYNKEPEYKDFRAGDVRHSQADITKAKTRLGYEPQFSISEGI
                     LKAMPWYVNSLGNKK"
     gene            2501..3589
                     /gene="rmlB"
     CDS             2501..3589
                     /gene="rmlB"
                     /note="RmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="putative dTDP-glucose-4,6-dehydratase"
                     /protein_id="AAO39692.1"
                     /translation="MKILITGGAGFIGSAVIRYIINHTNDSVVNVDKLTYAGNLQSLK
                     DIEKNTRYKFECADICDSAKMQEIFTNHQPDAVMHLAAESHVDRSINGPFEFIQTNII
                     GTFILLEIAKSYWLQLSESKKKKFIFHHVSTDEVYGDLAHPDVLDEGSPLPLFTEKNG
                     YAPSSPYSASKASSDHLVRAWYRTWGLPVIVTNCSNNYGPYHFPEKLIPLTILNALQG
                     QPLPVYGTGDQIRDWLYVEDHARALHLVISSKRVGETYNIGGYNEMKNIDVVQKVCEI
                     LDEIQPLSEGSYKKLINFVKDRPGHDRRYAIDATKITEHLGWQPIETFESGIRKTVHW
                     YLQNKAWVESVKNGNYKKWLTQQYGEMK"
     gene            3556..4455
                     /gene="rmlA"
     CDS             3556..4455
                     /gene="rmlA"
                     /note="RmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glucose-1-phosphate
                     thymidylyltransferase"
                     /protein_id="AAO39693.1"
                     /translation="MAYSAVWRNEMKGIILAGGSGTRLYPITKGISKQLMPVYDKPMI
                     YYPLSVLMLAGIKDILIITTPEDLNNFQRLLGDGKRFGINLSFEVQLKPEGLAQAFII
                     GGKFIGNDAVCLALGDNIFYGQNFSPKLKEAAQLIDGATVFGYQVKDPERFGIVEFDA
                     NKKALSIEEKPAKPKSNYAVTGLYFYDNNVVNIAKTIKPSERGELEITSINEVYLRNG
                     KLNVELLGRGFTWLDTGTHQSMLEASHFVETIEQHQGFKIACLEEIALYNGWLTKDEV
                     FKIGNEYKKNGYGQYLLSLVKDK"
     gene            4455..5558
                     /gene="vioA"
     CDS             4455..5558
                     /gene="vioA"
                     /note="VioA"
                     /codon_start=1
                     /transl_table=11
                     /product="putative dTDP-4-amino-4,6-dideoxyglucose
                     aminotransferase"
                     /protein_id="AAO39694.1"
                     /translation="MEKPIFVTQPNLPPLEEFIPYLEIIWQNKQFTNNGPMHQKLEKK
                     LCEFLGVEYISLFNNGTIALITAVQALGVKGEVITTPYSFVATAHSLVLNGLKPVFVD
                     IDSKTLNIDPRRIEEAITPETQAIMPVHCYGNPCDTQAIADIAQKYNLKVIYDAAHAF
                     GVEDDDGSVLRHGDLSVLSFHATKVFSTFEGGAIVCNSKEMKEKIDRLKNFGYIDETN
                     INIIGSNGKMSEVNAAFGLLQLEHMDTFLRGRMNADMFYRQKLKDITGISIVIPSGQK
                     ISNFSYFPILVESDFPLSRDELFNYLKNQNIFARRYFYPVIPDFQAYLNVGEVCDVKN
                     AREIASKVLCLPMHAELSSDILEYIVSTIREIK"
     gene            5555..6271
                     /gene="wbqC"
     CDS             5555..6271
                     /gene="wbqC"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="WbqC"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AAO39695.1"
                     /translation="MIAIMQPYLFPYLGYFQLITASDTFVLFDDVNYIKKGYINRNNI
                     LLNGSAYRFTVPVQNISQNKKINEHYFSDDTDNLLKTFNMAYAKAPYYMDIYPIIERA
                     LKGTERKVSSVCFNAINDILDYLGIEKNIVFSSNXDYDRCASATDKLIGITKALESNY
                     YINASGGQNLYDYDYFKSKNITLAFIQMDDIEYTQNGKNAEFIPNLSILDVLMWSSPK
                     NVLELLNKYTLITSGHNYGK"
     gene            6261..6683
                     /gene="wbqD"
     CDS             6261..6683
                     /gene="wbqD"
                     /note="WbqD"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acetyltransferase"
                     /protein_id="AAO39696.1"
                     /translation="MANDTLIFLSKVIQDDLNLKCSILNNYHKIDDYIQKLIEHATII
                     SIRENGQVIGAICFYCNNQKDLIAYVSMIAVHIDHRGKGIGDELLSFAIANCRRKGFE
                     LCKLEVNKRNVRALTLYSKNDFSILSESENTYLMQKKL"
     gene            6793..8187
                     /gene="wzx"
     CDS             6793..8187
                     /gene="wzx"
                     /note="Wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O antigen flippase"
                     /protein_id="AAO39697.1"
                     /translation="MGAESYGLIGFFTMLQALFGLLDLGLTPTIGRETARYHGGTMTV
                     LDYRKLLRTLHILFFTIATIGGVFLFFLAPIISVHWLKVTTISIDTVNYCVKIMAFSI
                     ALRWIGGLYRGIISGSERLDWLSYLNIFITTLRFIIIFPVMKVKGYTPFVFFTYQLIV
                     AIIEYLSLLCLSTLLTPKLKSANEKIGFSFSPIMSLMRFSLTVAFTSSIWILITQSDK
                     LILSGILELSEYGHFTLSVLLASGILMINIPVTNSILPRLARLHAENKHDELLRIYKN
                     TTMLVCILGVSASLVVAIYAEPLLLIWTGDAEVSASASPILSLYAIGNGLLAVSAFPY
                     YLQYAQGRLKYHFWGNIVMFFMLIPTIIVLARNFGGVGAGYAWLIVNVFYLFVWTALV
                     HHKLIPGLHLSWLMSIGMITIPTGIIVFFLSFIVRFSGNRLHDIIVLGLISILALMVS
                     IVSCWLIKRMNLGV"
     gene            8193..9083
                     /gene="wbqE"
     CDS             8193..9083
                     /gene="wbqE"
                     /note="WbqE"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyl transferase"
                     /protein_id="AAO39698.1"
                     /translation="MEGTVPKVSVCVITYNQAKYIKQCIESLITQDCDFDYEIIVSDD
                     CSTDNTREILEHLYHQYPEKIRIFIHEKNLGVTKNYLFLHEQAQGEYIAHVDGDDYYF
                     SNKLSLQARYLDENKECNIVWHPMLLDNNSRVFNGYQQSGTNFVDLKFTQGDIIQYIS
                     VGKNSSKMYRKTVRDIDIPAFELVDYLVNVEQVQNGYGGYASNEPLGVYRVGVGISSS
                     GDKTRIALRDTFLYLLKKYPKYRLEINTAALTYFIRDIFARRKSAKIFLHVWIKTFHP
                     FSVIKLLKGMSTIKKLKYRA"
     gene            9086..9607
                     /gene="wbqF"
     CDS             9086..9607
                     /gene="wbqF"
                     /note="WbqF"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acetyltransferase"
                     /protein_id="AAO39699.1"
                     /translation="MIKIVNEDLHAFTKNGSFASKVKCTIISHTFHLVLMIRLGQFLS
                     KIPVIGAFFRLVIEYSIRIIFSSDISLRARIGGGLVIMHGHDIVIGRDVVIGRNCKIL
                     NGVTLGNKDTESTENQQPVVGDNVIIGTGAKILGKVVIGNNVKIGANSVVISDIAPDS
                     VAVGIPARVTKGI"
     gene            9639..10829
                     /gene="wzy"
     CDS             9639..10829
                     /gene="wzy"
                     /note="Wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O antigen polymerase"
                     /protein_id="AAO39700.1"
                     /translation="MINLNPVAILTLLYLSINFLSMLIGCSSGEIQVETSIFRVSEES
                     LIYSFLLQAICLIFLYYIYKYFTNRISYPPLTFKAKWGRALLIIQIAFIIFNTQMGVN
                     TAGSVERIEGQSLSNYLFIILQPDILVAVISVCLNSGFLFWTNILVYLLSMFLRGWMG
                     GTFVILFLILSRYQNLRISLKTFLVSLCSLLLLFSILPALIEAKWAMRTGISLSVFIS
                     NMSSYVTPENYYAGINYLLNRFQHVGHLALIYENADDLFKKYNAGYFSSYYMDGIPQY
                     LLVKMYNLDMYKLSFYLVQYFFDITEPTWNINTGVVGWLYILRYESILFAFYIMLLLL
                     VPYYVVSRFAGKRMLSVLACFSIIYLFHGWLGAYVNLAFYACIISLLANIRLYRTVYI
                     PCEK"
     gene            10837..12729
                     /gene="wbqG"
     CDS             10837..12729
                     /gene="wbqG"
                     /note="WbqG"
                     /codon_start=1
                     /transl_table=11
                     /product="putative asparagine synthase"
                     /protein_id="AAO39701.1"
                     /translation="MCGLAGFLESNLNDNNSAKVLHSMGESIYRRGPDGTGIWFENIE
                     NKINVGFVHTRLAIIDLSDAGQQPMHSHCGRYVIAFNGEIYNHLELRYLLESNNHISW
                     RGHSDTETLLACFGIWGIDKTLQACVGMFAIALWDKKEHRLTLARDRVGEKPLYWGWQ
                     DDALLFGSELKALKTHPAFSAEINRDALCLLLRHNYIPAPHTIYRGIQKLQPGHYLSV
                     ELSNDERKETLHQYWSYEKVVQEGLVNPFMGSPIQAVEHLESLIAQSINGQLISDVPL
                     GAFLSGGIDSSLIVSIMQSLSSSPVKTFSIGFDDKKYDEAIFAVEVARHLGTDHTEMY
                     VNDKDIQDVIPLLPEVYCEPFADSSQLPTFLVSKMAQQHVTVALSGDAGDELFGGYTP
                     YSFTPKYWNYARNIPLQIRKFASRYIDKLPVNPKFKKLIECMAVQNPQLFYRNIISHW
                     LNPEELVKNSSEPSTAFSSSTTFPVNGGYVDWMMSVDAQVYMTDDILVKVDRAAMYNS
                     LETRVPLLDHRIIEFAWQLPVSLKINNGVGKWPLREILYKRVPKSMIERPKKGFSVPL
                     SSWLRGPLRDWAEELIAYKRLADEGYFNVAAVRSCWNLHIQGQADYSRKLWSILMFQS
                     WLENQK"
     gene            12684..13841
                     /gene="wbqH"
     CDS             12684..13841
                     /gene="wbqH"
                     /note="WbqH"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyl transferase"
                     /protein_id="AAO39702.1"
                     /translation="MEHTYVSIMVRKPEMKVVHIIIDLNVGGAELMLQRLIKHTADNN
                     VEHVVISLTDIGTLGEEILKSGVDVKCLNINSPIKYATSLLKLYKLFTKIKPDVVHTW
                     MYHSDLIGGISAKLAGVKKIIWCVRSTDISKGGNKLTLLIRWLCAKISSFIPDTIVYA
                     ANASKEVHEQCGYDKNKSLVIANGFDLTKLSPDKFSRSDLRKEIGLAENDVVVCSVGR
                     YSPVKDHSTFISAALQLAEEFSNVRFLLVGRGLTTQNKIIMTQLSVSCHSDKFILLGE
                     RSDVPACLNASDIFCLHSVTEGFPNVLGEAMAMGIPSVTSNVGDAAFLLDKVDFVVPA
                     GNSYLLAQKLREVILLSRKNRTKLGRILRLRIENEFSMDTVANKYLSLYKN"
     gene            13842..14966
                     /gene="wbqI"
     CDS             13842..14966
                     /gene="wbqI"
                     /note="WbqI"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyl transferase"
                     /protein_id="AAO39703.1"
                     /translation="MKKIVFIINNVDFLISHRLPILLEAQKNGFQVHVIAPNSRNNEL
                     LKKHKIMGHDLFLSRGGNNPFYDLFTLLQLTKILKFLKPDLVHLVTIKPTLYGGIAAR
                     IAKVPHVVAAVSGLGTVFLSRGIISGLRRLLVTTLYHSALKHKRIRVIFQNPDDRELL
                     VSAGILKVSNSCLIRGSGVDLREYPYLPEKVHGKTVVMASRLLRDKGVYEFIEAARLL
                     KQRNVEADIRIIGSPDTCNPTSITEAEISKWASENIVEFCGFRSDIAKQYSNANVICL
                     PSYREGLPKCLVEAAACGRAVVTTDVPGCRDAIVANVTGMLVAVRDPVSLADAIEFLL
                     KNPDERIKMGKAGRLLAENEYSIEHIVNQHLSIYNDLIHS"
ORIGIN      
        1 tattggtagc tgtaagccaa gggcggtagc gtgtttaaat aaatacatta gtactactac
       61 atattacatc attgtaggct atttaagcgc tacatgttaa gcgacagcgc tagcaatcaa
      121 atcttttaaa gttacttctc aggaatagta aaatgagctt taatcttgaa aattataaaa
      181 taggaattat tgggctgggt tacgttggct tacctttagc tgttgaattt ggcaaaaaaa
      241 taaagactct tggttttgat atcaacgaga aacggataaa agaattatca gcaggtaatg
      301 actttacgct tgaatgtagt tctgatgaac tggctcaagc ttactatctt caatattcat
      361 gtgattataa agaattagcg acttgcaatg tattcattgt gacggtacct actccaattg
      421 atgaatataa acaacctgat ttaacacctc ttgttaaagc ctcggaaaca ctcggaaaaa
      481 taatcaaaaa agatgatatt attatctacg aatcaactgt ttatccaggt gcaacagaag
      541 aagtatgtat cccagtaatt gagaagatct caggtctggt atttaatata gatttttttg
      601 ccggttacag cccggaacgc attaatccgg gtgacaaaga gcaccgcgtg acaacgatca
      661 aaaaagtaac ttctggttca acgcctgaaa ttgctgagat tgtcgataaa ttatatggaa
      721 ccatcatcac cgctggaacg cataaagcat cttcgatacg tgtggcggaa gcggctaaag
      781 tgattgagaa cacccagcgt gatttgaata ttgctctgat taacgaactt gcaatgattt
      841 tcaataagct ggggattgat acggaggaag tattaaaagc cgcaggcact aaatggaact
      901 tcttaccatt cagaccaggc ttagttgggg ggcattgcat cggagttgat ccctactatt
      961 tgactcataa agcgcaagcg ataggttatc atccagaagt catcctggcg gggcgtagaa
     1021 ttaatgatgg aatgagtaaa tatgtagcat cacagttcat taaagccatg ctcaagaaaa
     1081 aattccaaat tgaaggtgct aatgtcttaa ttatggggct tacctttaaa gaaaactgtc
     1141 cggatcttcg taatacaaaa gttgtagata tcttggctga actgaaagaa tacaacgtga
     1201 acgtggatat ttatgatcca tgggttgatg ttgaagaagc ctattatgag tatgggatta
     1261 atgtattaaa cacattacct gataagaaat atgatggagt gatctttgca gttgctcact
     1321 ctcaatttaa aaatatgagt aaagaagaag tccatggaat aattagaaat gatggtgtta
     1381 tttatgattt gaaatacatc attgacccta acttagtaga tattcgtctt taatcaatac
     1441 ttgatgcaat gtgtcagaga ggatgcctaa tgaaatataa aaaaatagaa gacgctttgt
     1501 cttctaaatc tgaaacatgg ttgattacag gtgttgcggg atttatcggt tctaacctat
     1561 tagaggcctt actaaaactt aatcaaaaag tagttggctt agataatttt gcaactggac
     1621 atcaagcaaa ccttgatgag gttaaagagt ctgtttcagc tgaacaatgg tctgctttta
     1681 cctttgtgga aggagacata tgtgagccag agacgtgtgt ggaggttgtt aaaggagttg
     1741 atcatgtcct acatcaggct gcattaggtt ctgtgcctcg ttcaattaaa gatccaatta
     1801 ctacaaataa taccaatata tcgggtttca ttaatatgtt ggttgccgcc aaagatgcca
     1861 atgtcagaag tttcacttac gctgcaagta gctcaacgta tggtgatcat cctgcattac
     1921 ctaaaattga agaaaatata ggtaatccgc tatctccata cgcagtgaca aagtatgtaa
     1981 atgaattata tgcgcaagta tttgcaagaa cctacggatt caaatcaatt ggattaagat
     2041 actttaatgt atttggaaaa cgccaggatc ctaatggggc atatgctgct gtgataccga
     2101 agtggactgc tgcaatgatc aatgatgaac cgctgtatat caatggtgat ggcgaaacaa
     2161 gtagagactt ttgttttatt gagaatgtcg tgcaaatgaa tattcttgct gcacagtctg
     2221 atgcgtctgc aagagatcag gtttataacg ttgcagttgg tgaccgtact acgcttaaac
     2281 aattattcac tgtattaaag aatgcactta atgaaaatgg tgtttcgtat aacaaagaac
     2341 ctgaatataa agattttcgt gcaggtgatg ttcgccattc gcaagctgat atcactaaag
     2401 ccaaaactag acttggatac gagccacagt tcagtatatc ggagggcatc ctcaaagcga
     2461 tgccgtggta tgtaaactct ctgggcaaca aaaaataacc atgaaaatcc ttattacagg
     2521 tggtgctggt tttattgggt cagctgttat tagatacatc attaatcata ctaatgacag
     2581 tgtcgtaaat gtcgataaat tgacttacgc aggaaacctt caatcactta aagatataga
     2641 aaaaaatacg cgctataaat ttgagtgcgc agatatctgc gatagcgcaa agatgcaaga
     2701 gatatttacc aatcatcaac cagatgctgt tatgcatttg gccgctgaga gtcatgttga
     2761 tcggtcaatt aatggcccgt ttgagtttat acagaccaat atcataggca cctttatctt
     2821 acttgaaata gccaagtctt actggctgca acttagtgaa agcaaaaaaa agaagtttat
     2881 ttttcatcat gtttcgacag atgaagtgta tggtgatcta gctcatccag atgtactgga
     2941 tgaaggttca ccattgcctt tatttactga aaaaaatggc tacgcgccaa gcagtcctta
     3001 ttcagcctcc aaagcatcta gcgatcattt agttcgtgct tggtacagaa cttgggggct
     3061 tcctgtaata gtaacgaatt gttccaataa ttatggtccc tatcatttcc ccgaaaaatt
     3121 aatcccattg acgatcctta atgcgctgca aggtcagcca ttgccagttt atggcacagg
     3181 ggatcaaatc cgggattggt tatatgttga agaccatgct agggcacttc atcttgtcat
     3241 cagcagtaaa cgtgttggag aaacatataa cataggtggc tataatgaaa tgaagaatat
     3301 tgatgtcgtt cagaaggtct gcgaaatatt agatgaaatc caaccattga gtgaaggatc
     3361 ttacaaaaaa ctgattaatt ttgtaaaaga cagaccgggg catgaccgtc gctatgccat
     3421 tgatgctacc aaaattacag agcatttagg ttggcaacca attgagacat ttgagtctgg
     3481 tattagaaaa acagttcatt ggtatctaca aaataaagct tgggttgagt cagttaaaaa
     3541 tggaaattat aaaaaatggc ttactcagca gtatggagaa atgaaatgaa aggaataatc
     3601 ctcgccggtg gctctggtac aagactttat cccatcacga agggaatatc aaaacagttg
     3661 atgcctgttt atgataaacc tatgatctat tatccacttt ctgtattaat gctagcaggt
     3721 atcaaagata tattaatcat aacaacgcct gaagatctga ataattttca gcgattactc
     3781 ggcgatggta aacgttttgg tattaatctc tcatttgagg tgcagttaaa acctgaaggg
     3841 ttagcacagg ctttcattat tggggggaaa tttataggca atgatgcggt ctgtttggca
     3901 cttggagata atatttttta tggacaaaac tttagtccaa aactcaaaga ggctgcacaa
     3961 ttaattgatg gtgcaaccgt atttggatat caggtaaaag atccagaacg atttggtatc
     4021 gtagagtttg acgctaataa aaaagcactg tctatagaag aaaaaccggc aaaaccaaaa
     4081 tcaaattatg cagttacagg tctttacttt tatgataata acgtagttaa cattgccaaa
     4141 acaattaaac cttcagagcg cggcgagtta gagattacca gtataaacga agtctatctt
     4201 cgcaatggga aattgaatgt tgagttgttg gggcgaggct ttacctggct tgataccgga
     4261 acacatcaaa gcatgttgga agcatctcat tttgtggaaa ctatcgaaca acatcaaggt
     4321 ttcaaaatcg catgtctaga agaaatcgcc ttgtacaatg gatggctaac caaagatgaa
     4381 gtattcaaaa taggtaatga gtacaaaaaa aatggttacg gtcaatatct tctctctttg
     4441 gttaaggaca aataatggaa aagccaatct ttgtaacgca acctaattta ccaccgctag
     4501 aggagtttat accatatctg gaaatcattt ggcagaataa gcaatttaca aataatggtc
     4561 caatgcatca aaaattagaa aaaaaattat gcgagtttct tggtgttgaa tacattagtc
     4621 tatttaataa tgggactatt gcgcttataa ccgcagtaca ggctttgggt gttaaaggcg
     4681 aagtaattac cacaccatat tcctttgtag caactgcaca ctccttggtc ttaaatgggc
     4741 ttaaacctgt ttttgtcgat attgattcca aaaccttaaa tatcgatccg agaagaattg
     4801 aagaggcgat tacccctgaa acgcaggcaa taatgccggt gcactgctat gggaatcctt
     4861 gcgatacaca agctattgct gatattgcgc aaaaatataa tttaaaggtc atttatgatg
     4921 ctgcgcatgc ctttggcgtt gaagatgatg atggaagtgt tcttcgccat ggagatctaa
     4981 gtgtcttaag tttccatgca actaaagtgt tcagcacttt tgaaggcgga gctattgtgt
     5041 gtaatagtaa agaaatgaaa gaaaaaattg atagactaaa aaactttggt tatatcgatg
     5101 aaactaacat caatatcatt ggctctaatg gaaaaatgag cgaagttaat gctgctttcg
     5161 gcttgctaca attagaacat atggatactt ttctacgtgg tcgaatgaat gctgacatgt
     5221 tttatcggca gaaacttaaa gatatcactg gtataagcat agtaattccc agcggccaga
     5281 aaatatcgaa tttttcatat ttccctatat tggttgaatc tgattttccg ttatctcgtg
     5341 atgaactatt taattatctg aagaaccaga atatttttgc aagacgttat ttttatcctg
     5401 ttataccaga ttttcaagcg tatttgaatg taggtgaagt ctgtgatgtc aagaatgccc
     5461 gtgaaatagc ctcgaaagtg ctttgcctac cgatgcatgc agaattgagc tctgatatct
     5521 tagaatatat tgtaagtacg attagggaga ttaaatgatt gccataatgc agccctattt
     5581 atttccatat ttagggtatt tccaattgat cacggcatca gatacattcg tgctttttga
     5641 tgatgttaat tatataaaga aaggatatat caacagaaac aacattttgc taaatggatc
     5701 tgcatatagg tttacggttc ctgtgcaaaa tatatcacag aataaaaaaa ttaatgagca
     5761 ttatttttct gatgacactg ataatctatt aaaaaccttt aacatggcat atgcaaaagc
     5821 tccatactat atggatatat atcctattat agagagagct ttaaaaggaa cagagagaaa
     5881 ggttagttct gtttgtttca atgctattaa cgatatactg gattatctcg ggatagaaaa
     5941 aaatattgtc ttctcaagta ataktgatta tgacagatgt gctagtgcaa ctgataaact
     6001 aattgggata acaaaagcat tagaaagcaa ttactatatt aatgcaagtg gtggacaaaa
     6061 tctctacgat tacgattatt ttaaaagcaa aaatattacg ctcgcgttta ttcaaatgga
     6121 tgatattgag tatacacaga atggaaagaa tgctgagttt attcctaact tgtcaatatt
     6181 agatgtctta atgtggtctt ctccaaaaaa tgtgttggaa ttgctaaata aatatactct
     6241 gataacaagc gggcataatt atggcaaatg atacgttgat ttttttaagt aaagttatcc
     6301 aagatgatct aaacctgaaa tgttcgattc ttaataacta tcacaagata gatgactaca
     6361 ttcaaaaatt aatcgaacat gctacaataa tttcaatcag agagaatggt caagtaattg
     6421 gtgctatctg tttctattgc aataatcaaa aagatctaat tgcgtatgta agtatgattg
     6481 cagttcatat tgatcataga gggaaaggaa tcggcgacga gcttctaagt tttgcaattg
     6541 ctaattgtag aagaaagggg ttcgaacttt gcaagttaga agtcaacaaa agaaatgtta
     6601 gagcattaac cctttacagt aaaaatgatt tttcaatatt atcagaatct gagaatactt
     6661 atttgatgca gaaaaaatta taaataggct ttgaatattg tgtctttaaa aaaaaatatt
     6721 atcattagct atgctgcaca aatatatgtc agtgctgtcg ggattttaat actccctctg
     6781 tacatcaagt acatgggagc agagtcatat ggattaattg gcttttttac tatgcttcag
     6841 gcgctgtttg gtctcttaga cttagggcta actccaacaa ttggtcgtga aacagctcgc
     6901 tatcatggcg ggacaatgac agtgctggac tacagaaaat tgctaagaac tcttcatatt
     6961 ttgttcttca ctatcgctac tattggtgga gtatttctat ttttcttagc acccatcatt
     7021 tcggttcatt ggttaaaggt tacaacaata tcaattgaca ccgtaaatta ctgtgtaaag
     7081 ataatggcat tctctatagc attacgatgg ataggtgggt tatatagggg aatcattagc
     7141 ggtagcgaaa ggttagactg gctaagctat ttaaatattt ttataacaac gcttagattc
     7201 atcattatat ttcctgtcat gaaagtaaaa ggttatactc ctttcgtatt tttcacatat
     7261 caattaatcg ttgcgatcat tgagtatttg tctttattat gtttgtctac tttattaact
     7321 cctaaattga aatcagcaaa cgagaaaatt ggtttttcat tttcgcctat tatgtcactg
     7381 atgcgctttt ccttaacagt tgcttttact tcctctattt ggatattgat tactcagagt
     7441 gataaactca tcttgtcggg tatattggaa ttaagtgaat acgggcattt cacactttct
     7501 gtgttgttgg ctagtggcat tctgatgatt aatattccgg ttacaaactc tatattaccg
     7561 aggcttgcgc gtttacatgc tgaaaataag catgatgaac ttctccgtat ttataaaaat
     7621 acgactatgt tagtatgcat cttgggagta agcgcatcgc tagttgttgc tatctatgca
     7681 gaaccattgc ttcttatctg gactggtgat gcagaagttt cagcaagtgc atcgcctatt
     7741 ctctcattgt atgccattgg aaatggttta ctggcagtat cagctttccc atattatcta
     7801 caatatgctc aagggcggct aaagtatcac ttctggggaa atattgtaat gttcttcatg
     7861 ctaatcccca caattattgt acttgctcgt aattttggtg gagttggcgc gggttatgcc
     7921 tggttaatag ttaatgtctt ttatcttttt gtatggactg cattggttca tcataaatta
     7981 atacccggat tacatctctc ttggttaatg agtatcggta tgataacaat tcctaccggt
     8041 attattgtat tttttctctc ctttattgtc cgatttagcg gtaatagatt gcatgacata
     8101 attgtgctgg gcttaatatc aattttggct ctgatggttt caatagtatc atgctggcta
     8161 atcaaaagaa tgaatctcgg ggtgtaaaat aaatggaagg aacagttcct aaggtctcag
     8221 tctgtgtgat aacatataac caggcgaaat atataaagca atgcatagaa agcctaatca
     8281 ctcaggactg tgatttcgat tatgaaataa ttgtgagcga tgattgttcg acggataata
     8341 cacgagaaat tttagaacac ttatatcatc aatatccaga aaagatacgt atatttatac
     8401 atgaaaagaa tcttggggtt actaaaaatt atcttttcct gcatgaacag gcgcaaggcg
     8461 agtatattgc acatgttgat ggtgatgact actatttttc aaataaatta agtttacaag
     8521 cacgatatct tgatgaaaat aaagagtgta atattgtttg gcatcctatg ttgttagata
     8581 ataattcacg agtatttaat gggtatcagc agagtggaac taattttgtc gatttaaaat
     8641 ttactcaagg tgacataatt caatatattt ctgtcggtaa aaacagctca aagatgtatc
     8701 gaaaaaccgt acgagatatt gatatacctg cattcgagct cgttgactac ctcgttaatg
     8761 ttgaacaagt tcagaatggc tatggtggat atgcttcgaa tgaacctctg ggagtatatc
     8821 gagtaggtgt aggaatttca tcttctggag acaaaactcg cattgctctt cgggatactt
     8881 ttctttatct attaaaaaag tatccaaaat acagattaga aataaataca gcggccttga
     8941 catattttat ccgcgatatc tttgcaagaa gaaaatctgc aaagattttt ttacatgtat
     9001 ggataaaaac gtttcatccc ttttcagtaa ttaaattact taaggggatg agtacaatta
     9061 aaaaattaaa atatagagca taagtatgat taaaatcgtt aacgaggact tacatgcttt
     9121 tactaaaaat ggcagtttcg ctagtaaagt taaatgcaca attataagcc atacttttca
     9181 tctagtgctc atgatacgct tagggcagtt tctatcaaag atacctgtga tcggcgcatt
     9241 ttttagattg gtcattgaat attccataag aatcatattt tctagtgata taagtctaag
     9301 ggcaaggatt ggtggtggat tagtaataat gcatggccat gatatcgtta ttggaagaga
     9361 tgttgtcata ggcagaaatt gtaaaattct gaatggcgtc actttaggta ataaggacac
     9421 ggaatcaaca gaaaaccagc aaccagtagt gggtgataat gtgataattg gtactggagc
     9481 taaaattctg gggaaagtag ttataggaaa taatgttaaa attggagcta atagtgtagt
     9541 tatttctgac attgctccag attctgttgc tgttggtata cctgcaaggg ttactaaagg
     9601 tatctaatag gttaagataa gtaacgaaga gattcatcat gataaatcta aatccagtag
     9661 ctatcctaac cttactttat ttatctatca attttttatc aatgttgata ggatgttcta
     9721 gtggtgaaat acaagtagaa acatcaattt ttagagttag tgaagaaagt ctaatttatt
     9781 catttttact tcaagctatc tgtcttattt ttttatatta catttataaa tattttacaa
     9841 atagaatctc atatccccca ttaacattta aagcaaagtg gggaagggcg ttacttatca
     9901 ttcaaatcgc atttattatt tttaacacac aaatgggcgt taatacagcc ggtagtgttg
     9961 aaaggataga aggacaatcg ttaagcaact atctttttat aattttacaa cccgatattc
    10021 tagtagccgt tatttcagta tgcttaaatt caggtttcct attttggact aatatcttag
    10081 tatacctgct atccatgttc cttagaggtt ggatgggtgg aacctttgtc atactctttt
    10141 tgatattatc acgttatcaa aatttaagga tctcactaaa aacttttttg gtatccttat
    10201 gttctctatt gcttcttttc tccattttgc ctgcactcat tgaagcgaaa tgggcaatga
    10261 ggaccggtat atctctatca gtatttataa gcaatatgag ttcatatgtt actccagaga
    10321 attattatgc tggcataaac tatctgttaa atagatttca acatgtcggc catttagcgt
    10381 tgatatatga aaatgcagat gatttattta aaaaatataa cgcaggttat tttagctcat
    10441 attatatgga tggcattcct cagtatcttc ttgttaagat gtataactta gatatgtata
    10501 agctcagctt ttatcttgtt caatatttct ttgatataac agaaccgact tggaatatta
    10561 acacgggcgt ggttggatgg ctttatatat taaggtatga atccatactt tttgcgtttt
    10621 atataatgtt attgttgctg gttccttatt atgtagttag tagatttgct ggtaagagaa
    10681 tgttgagtgt tttggcttgc ttttctatta tttatttatt tcacggatgg ttgggagcct
    10741 atgttaactt agcattttac gcatgtataa taagtctgct tgcaaatata agattataca
    10801 gaactgtata tattccatgt gagaaatagg attgttatgt gtggattagc tggtttcctt
    10861 gaatcaaatt taaatgataa taatagtgct aaagttttac attcgatggg tgaatccata
    10921 taccgaaggg gaccagatgg tacaggaata tggtttgaaa acattgagaa taaaataaat
    10981 gttggttttg ttcatactcg attagctatt attgatctct cagacgccgg tcaacagccg
    11041 atgcattctc actgcggtcg ttatgtaatt gcattcaatg gtgaaatata taatcatctt
    11101 gaactaagat atctattaga aagtaataat catatttcct ggcgtggcca ttctgatacg
    11161 gaaacgcttt tggcatgctt tggtatatgg ggtattgata agacattgca ggcatgtgtt
    11221 ggaatgtttg caatagcctt atgggacaaa aaagaacatc ggttaacatt agcacgtgat
    11281 agagttggtg aaaaaccgtt gtactgggga tggcaagatg atgcccttct ttttggctca
    11341 gaattaaaag cgttgaaaac tcatccggca tttagtgcag aaataaacag agacgcactt
    11401 tgcttgctat taaggcataa ctatattcct gcaccacata caatatatcg agggatccaa
    11461 aaactacaac cgggccacta tctaagtgtt gagttatcaa atgatgaaag aaaggagaca
    11521 cttcatcagt actggtcata tgagaaagta gtacaagaag gattagtgaa tcctttcatg
    11581 ggatcaccta tacaagcagt agaacatctt gagtctttaa tcgctcaaag tattaatgga
    11641 cagttaattt ctgatgtccc tcttggcgct tttcttagtg gtggaattga tagtagtctg
    11701 attgtgtcta tcatgcagtc tttatcatca tctccagtga aaacattctc tataggattt
    11761 gatgataaaa aatatgatga ggctatcttt gctgttgaag ttgcccgtca tttaggtacc
    11821 gatcacaccg aaatgtacgt aaatgacaaa gatatacagg atgttattcc acttttgcct
    11881 gaagtgtatt gtgaaccttt cgctgatagt tctcagttgc caactttttt ggtcagtaaa
    11941 atggcacagc aacatgttac cgtcgcgttg agtggtgatg caggtgatga gttatttggc
    12001 ggatatacgc catatagctt tacgccaaag tattggaatt atgcgcgaaa tataccattg
    12061 caaattagaa aatttgcttc taggtatatt gacaaattgc cagttaatcc gaagtttaaa
    12121 aaattaatag agtgtatggc agttcagaat ccgcagcttt tttacaggaa tatcattagc
    12181 cattggctta atcctgaaga gttagtcaaa aactcgagtg agccatcaac tgcatttagt
    12241 tcatctacta cgttccctgt aaatggcgga tatgttgact ggatgatgtc ggtagatgct
    12301 caagtctata tgacagatga tattttagtc aaagttgaca gggctgctat gtataatagc
    12361 ctcgaaaccc gagtgccatt attggaccac cgtattatcg aatttgcttg gcagttaccg
    12421 gtttctttaa agattaataa tggtgtaggt aaatggcctc tgcgagaaat actctataaa
    12481 cgagtaccga aatcaatgat tgaacggccg aaaaaaggat tctctgtacc gctatcatcg
    12541 tggttacgcg gacctttgcg tgattgggcg gaagagttga ttgcttataa acgtctcgca
    12601 gatgaaggat attttaatgt tgctgctgtg agaagttgct ggaatctgca tatccaggga
    12661 caggctgact attcaagaaa attatggagc atacttatgt ttcaatcatg gttagaaaac
    12721 cagaaatgaa agtcgttcat attataatcg atttgaatgt tggtggggcg gagctcatgc
    12781 ttcagcgtct tattaagcat accgctgata ataatgttga acatgttgtc atctctctta
    12841 ctgatattgg cacactcggt gaggaaattc taaagagtgg ggtagatgta aagtgcttaa
    12901 acattaattc cccaattaag tatgccacca gtttattgaa gctttataag ctatttacta
    12961 aaattaagcc tgatgttgtt catacctgga tgtatcacag tgatcttatt ggtggtatct
    13021 ctgcgaaatt agccggcgtt aaaaaaataa tttggtgtgt tagaagtact gacattagta
    13081 aaggaggaaa caagcttacg cttctgataa gatggttatg cgctaaaata tcatcgttta
    13141 ttcctgatac tatcgtttat gctgcaaacg cttcgaagga agttcatgaa cagtgcggtt
    13201 atgataagaa taaatcgttg gtcattgcca atggatttga tttaactaaa ctatcgccag
    13261 ataaattttc cagaagcgat ttaagaaaag aaatcggctt agcagaaaat gatgttgtgg
    13321 tatgttcagt tggtcgctat agtccagtaa aagatcactc aacctttatt tcagcagctc
    13381 ttcaacttgc agaagaattt agtaatgtac gcttcttatt agtcggtcgt ggcttgacca
    13441 cccagaataa aataataatg acacagctta gtgtaagctg tcactcagat aaatttattc
    13501 ttcttggtga gcgtagcgat gttcctgcat gtttaaatgc ttctgatatt ttttgccttc
    13561 attcagtaac agaaggcttc cctaatgtct taggagaagc aatggcaatg ggaattcctt
    13621 ccgtgacttc aaacgttggt gatgccgcat ttttacttga taaggttgat tttgttgttc
    13681 cagccggaaa ctcgtattta ttagcacaga aactgcggga agttattttg ttaagtcgaa
    13741 aaaaccgcac taagttaggt cgtatattaa ggctgcgaat tgagaatgag ttttctatgg
    13801 atactgtagc caataaatat ttatccttat ataaaaatta aatgaaaaag attgttttta
    13861 taatcaataa tgtcgatttt ttaatatctc atcgattacc tatcttactc gaggctcaga
    13921 aaaatgggtt tcaggttcat gtgattgctc ctaattccag gaataatgag ctactaaaga
    13981 agcataaaat aatgggtcat gatctctttc tttccagagg aggtaataac cctttttatg
    14041 atttatttac tttgcttcag cttacaaaaa ttctgaaatt tctcaagccc gaccttgtcc
    14101 atcttgtcac gattaaacca acactttatg gtgggattgc tgccagaatc gccaaagtgc
    14161 ctcatgtcgt tgcagctgtc tctgggctgg gaaccgtatt cttgagcaga ggaatcatca
    14221 gtggcttacg tcgcttactg gttacaacct tgtatcactc cgctctgaaa cataaaagaa
    14281 tacgcgttat ctttcagaac cctgatgatc gtgagttact tgtgagcgca ggcatattaa
    14341 aggtttccaa ttcctgtctg atcagaggtt cgggggttga tttaagagag tatccttatc
    14401 ttcctgaaaa agttcacggt aaaactgtag taatggcctc caggctacta agggataaag
    14461 gggtttatga gtttatcgaa gccgctcgct tacttaaaca aaggaatgta gaggctgata
    14521 ttagaatcat tggttctcca gatacctgta atccgacaag tatcactgag gctgaaataa
    14581 gcaaatgggc atcagaaaat attgttgaat tctgcggatt tagaagtgac attgctaagc
    14641 agtactctaa tgctaatgta atatgcttgc cctcatatcg agaagggtta cctaaatgtc
    14701 tggttgaagc cgcagcgtgt ggtagggcag ttgttacaac tgatgtacca ggttgcaggg
    14761 atgcgatagt ggcaaatgtt actgggatgt tagtcgcagt tcgggatcct gtatcgttag
    14821 cagatgcgat tgagtttctg ctaaaaaatc cagatgaaag aataaaaatg gggaaggcgg
    14881 ggagattgtt agctgaaaat gagtactcaa tcgaacatat agtgaatcag cacttatcca
    14941 tttacaatga cttaattcac tcctgacgct tagtgctggt gccatttatg atcattcttt
    15001 ttgcatccta tttggcactt gatgctatcc atcgcgccct aacctaagtt aacatcttca
    15061 atacattcaa gccgcgcata cgtcgcgtgg tgaccacacc tgacaggagt atgtaatgtc
    15121 aaagcaacag atcggcgtcg tcggtatggc agtga
//

</pre>