LOCUS       AB812052               19924 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: 35w.
ACCESSION   AB812052
VERSION     AB812052.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 19924)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..19924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="35w"
                     /db_xref="taxon:562"
                     /note="serogroup:O120"
     gene            <1..863
                     /gene="wcaM"
     CDS             <1..863
                     /gene="wcaM"
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01573.1"
                     /translation="ANITNCKFSDLQGDAIEWNVAINDSDILISDHLIERINCTNGKI
                     NWGIGIGLAGSTYDNNYPENQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNI
                     TPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNFRVND
                     IQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINVMQES
                     SVGPALSMNFDMRKDVRGVFMAKRKTLLSLTNIHAVNEKEQSSVDIDRINHHIVNVEK
                     INFRLPERRE"
     CDS             complement(921..1826)
                     /note="O120_01;
                     transposase of IS2"
                     /pseudogene="unknown"
                     /codon_start=1
                     /transl_table=11
     CDS             complement(1784..2194)
                     /note="O120_02"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase of ISEc27"
                     /protein_id="BAQ01574.1"
                     /translation="MIVLILVFRLVIGEQMIDVLGPEKRRRRTTQEKIAIVQQSFEPG
                     MTVSLVARQHGVAASQLFLWRKQYQEGSLTAVAAGEQVVPASELAVAMKQIKELQRLL
                     GKKTMENELLKEAVEYGRAKKWIAHAPLLPGDGE"
     gene            2357..3352
                     /gene="gne"
     CDS             2357..3352
                     /gene="gne"
                     /note="O120_03"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4-epimerase"
                     /protein_id="BAQ01575.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALVGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            3595..4488
                     /gene="galF"
     CDS             3595..4488
                     /gene="galF"
                     /note="O120_04"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01576.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGTKFRKGIEKLLSE"
     CDS             complement(4525..4788)
                     /note="O120_05"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BAQ01577.1"
                     /translation="MHATAPGFTATRALHACLRCDELYPLAPNPKNSNANCLTNPLWK
                     QGKSWKTLTKTLLLTRCYSDGLYKTTAGIFATNYFCCKSSLPL"
     gene            4861..5946
                     /gene="rmlB"
     CDS             4861..5946
                     /gene="rmlB"
                     /note="O120_06"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6 dehydratase"
                     /protein_id="BAQ01578.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAVMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKADETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            5946..6845
                     /gene="rmlD"
     CDS             5946..6845
                     /gene="rmlD"
                     /note="O120_07"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="BAQ01579.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATGVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALIKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            6903..7781
                     /gene="rmlA"
     CDS             6903..7781
                     /gene="rmlA"
                     /note="O120_08"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="BAQ01580.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            7786..8346
                     /gene="rmlC"
     CDS             7786..8346
                     /gene="rmlC"
                     /note="O120_09"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="BAQ01581.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCAVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFLYKTTNYYHPESDRGIIWNDPNIGIVWPER
                     ENIVLSKKDINQPNLSYSFSLKQLLS"
     gene            8359..9840
                     /gene="wzx"
     CDS             8359..9840
                     /gene="wzx"
                     /note="O120_10"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="BAQ01582.1"
                     /translation="MSLLSNAKWVSLSQFGRIIIQLISLTVLTRFIPPADYGLMALGT
                     VVMNFVFIIRDLGTASAIIQRKEVDDDILNSVFWLNVTMGVGLCILIILCTPLIELLF
                     KNEKLDNILYLLCFCFPLASIGSAHQALMERNSHFKKIAIIELLSSLSGFVVALLVAI
                     NNGGVYALVYQLICSSFISSILFFYLSSWTPSLHFKFEGVKKIIGFSSRLTVFNLVNY
                     FSRNADAMIISRKYDANILGAYSLAYRIMLFPLQSLTFIASRSLYPILSRNQDDLKLI
                     REIYLKTIFIISMITIPLMLGLVFLRTPFITIALGPNWAITSNILLWLAPTGIIQSIL
                     SCSGSILMSTNNTKTLMLLGIFGAAIQVLSFFIGSLFSINIFVFAYFIANVINALVVL
                     YFTFKSIKLNIYSFCKILLIPTLSCSVMIIALHFVKKIIYINNVVNDVSYLIIQVIIG
                     CIVYFIVFYIFVNICLRFKIMSENMIPDFLLRIKFFSHKRFLN"
     CDS             9837..10682
                     /note="O120_11"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ01583.1"
                     /translation="MNKQLFLIVVYNKKIAESCTFNKVVNSKFSDDSLCVIWNNGPTS
                     CEEEFLNVYEYNKNIRYHETLHNISLAKIYNEVINKYNAQIYIILDDDSSLTDAYFEA
                     VKQISPNEVGMPVIYYDRKIINPCINGQIYSKGINIDANDTITTIGSGLVVGRDVASQ
                     LFKAYNSVFDERFYLYGVDTTFCFRLNKLKINDKIKIIDGFEHGLSRLEGKNDKLTLF
                     RKIERSNDLALQIRYYKEKKHWFLMMVLLLASSIKKFITFQPQQYKYSTIIKTFVKGK
                     HERQR"
     gene            10666..11925
                     /gene="wzy"
     CDS             10666..11925
                     /gene="wzy"
                     /note="O120_12"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="BAQ01584.1"
                     /translation="MNVRDNDNAIKLLEFVLIFSFFIIALFFLYSPSYLIYIVVSAFV
                     LIKFNNNYSFGLFILLIMVWLIVNSTNVISYQNFVLASDDFSSYYNNYLGFLHYGINE
                     ENLFEFGYSEIMLPLLNYIASLIIGQPEPYKLKFLYSVLQSTGVLYCCVKISRHYKLD
                     LSNSILLTAAVLVFYKPLVGIQLSRQMFSSIFIIAFIFTNNKFERIIFSLMAFCFHSS
                     AIILLPITQYLLTKRKRKDFYYSLIIVLLAYAAFIVVFTSFKNELINIPILNKLDYTF
                     RMFNEPEKIKQSVFTSFLLLIYLIPALIFSILNKKSDGFKYNLAIICVFLISFCIVPG
                     FNNRLFFAELYVFLGFFYYMVFFYNRVNAREHVSRLIIFILFTILFFQSQFSSENRQF
                     DSFELEPFYYLKYLEYRETEINRGLLE"
     CDS             11927..12718
                     /note="O120_13"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ01585.1"
                     /translation="MPNLVIYTAVTGHYDELGNVYEEPSVDYICFTDYNFTGTVPKPW
                     KQIKLPPSNFSDKDLARYCKLNPHVLLPQYQHSLWIDGNIRIKGKIRNFIIDILSKHR
                     IAAYEHWWRDKTEQEFHECARSGFDPAWKLYKQIERYKHEGYTSSDFFENNILMRNHM
                     ESDIIKMHEIWWGEYISGGKRDQYSFTYAAYKSDVNIYSLGVHDARVIKQYFDYVTHS
                     KKRPLKQYIWIIINRIYISLTRWSVNEPSRTNKLVLNNERDSNEK"
     CDS             12693..13613
                     /note="O120_14"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ01586.1"
                     /translation="MKEIQMKNKVAGVIVTYNPEIKILLSALESSTKQLDYIYLIDNC
                     SLNKNEVADLAGRFGNVKFQALSKNIGLASAQNMGINTAKNNNLIEYVVLFDQDSIIE
                     NNFIASLLDDYKKVLAKDSKIGAIGPVFYDPSTKINYPATVYIGPFIKKISIKDEPQF
                     ATYLIASGCLIKIDTLSSVGLMQDDLFIDYIDVEWSLRAKSKGYNLYISPNAKMAHTI
                     GDARRKFLGRTISIHSPFRRYFLVRNSFYMIRLNYIPLGYKTRECIFNIARILTSIIY
                     SDQKIKTIKSAFYGVIDGIAGRFGPLKRKI"
     CDS             13625..14728
                     /note="O120_15"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ01587.1"
                     /translation="MKILYIITKADEIGGAQIHVRDLARRLHNDGNKVTVIVGEDGAL
                     VRQLKELHISVRIVENLVRQISPLNDIKAIFNIRKIIKDNSPDIIGLHSSKAGIVGRL
                     AACRLNIPVVFTAHGWAFAEGISDKKRKLFIFIEKILSPFLDKIITVSEQDKKLALDL
                     GVASSEKQVVIHNGMPDITIKRKDYSDSTKVRLISVARFSEQKDHETLFHALKLISTD
                     NWTLTLVGKGPKLENIKKLASDLNIANNILFLGERDDVDNLLSESDVFCLITNWEGLP
                     LSIIEAMRASLPVVATDVGGVSELVEHKKTGLLHSPKNIQELANILDTIINDSDLRYK
                     LGNAGRVKYQNNFTFEHMYYATIDEYNKLIHTK"
     gene            14886..16292
                     /gene="gnd"
     CDS             14886..16292
                     /gene="gnd"
                     /note="O120_16"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01588.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPVGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            16541..17707
                     /gene="ugd"
     CDS             16541..17707
                     /gene="ugd"
                     /note="O120_17"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01589.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            17797..18831
                     /gene="wzz"
     CDS             17797..18831
                     /gene="wzz"
                     /note="O120_18"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ01590.1"
                     /translation="MAIIAKLWRLKIILSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGS
                     AAPKVSEIQSMLIGRFSTTFSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAKYIQQVDDQVNEELEQDLKDNIALQMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQIQQTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQT
                     RQNLLDIDNLDVDKLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(19017..19628)
                     /gene="hisI"
     CDS             complement(19017..19628)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01591.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLHQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
ORIGIN      
        1 gtgccaacat caccaactgt aagttcagcg acttacaggg cgacgctatt gaatggaacg
       61 tggcgattaa cgacagtgat attttgatat ccgaccatct cattgagcgc attaactgta
      121 ccaacggcaa aatcaactgg ggcatcggca taggccttgc gggaagtacc tacgataata
      181 attatccgga aaaccaggca gtgaaaaact ttgtcgtggc gaatatcacg ggatcggatt
      241 gtcggcaact gatccatgtt gaaaatggta aacattttgt tatccgtaat atcaaagctc
      301 gtaatatcac gccggatttc agtaagaaag caggtattga taacgccaca gttgctattt
      361 atggttgtga caactttgtg attgataata ttgaaatgat taatagtgcc gggatgttaa
      421 tcggctatgg ggtaattaaa ggcaaatatc tctcgatacc gcagaatttc cgagtgaatg
      481 atattcaact ggataatact catcttgctt ataaattgcg cggcattcaa atctccgccg
      541 ggaatgctgt ctcctttgtg gcgctgacta acattgagat gaagcgtgcg tcgctggagt
      601 tacacaacaa accgcaacat ctttttatgc gcaatatcaa tgtgatgcag gaatcctcag
      661 ttggacccgc attgagcatg aactttgaca tgcgcaaaga cgttcgaggc gtttttatgg
      721 caaagcgtaa aacgctgctt tcactcacga atattcatgc agtgaatgaa aaagagcaaa
      781 gctccgtcga tatcgacagg attaatcacc atattgttaa tgtggagaag attaacttta
      841 gattgccgga acggagggag tagatttgcg accattcata aacttactgg atataatgtt
      901 ctaaaagatt ggatttgccc ctatatttcc agacacctgt tatcacttaa cccattactg
      961 gcctgctgcc gcagatattc ccgtggcgag cgataaccca gtgcactatg cggatgccat
     1021 tcgttataat gctcgaacgc ctctgcaagg ttctttgctg ccgttaaccc gtctggttta
     1081 ggcatgatac tgatgtagtc acgctttatc gttttcacga agctctctgc tatgccgtta
     1141 ctctccggac tccgcaccgc cgtgttcttc ggttcaagcc ccaacatccg ggcaaactgc
     1201 cgtgtttcat tagcccggta gcatgaacca ttatccgtca gccactctac tggagacgcc
     1261 ggaagctcgt tgccgaagcg gcgttccacc gctcccagca ttacgtcctg tactgtttca
     1321 ctgtcgaagc cgcccgtagt gactgcccag tgcagtgcct cacggtcaca gcagtccagc
     1381 gcgaacgtga ctcgcagttt ttctccgtta tcacagcgga actcgaaccc gtcagagcac
     1441 catcgttgat tgctttcttt cacggccact ttgccagtat gtgcccgttt cgatggcggt
     1501 acagcggttt ttcgctcaag caacagcgca ttctggcgca tgatccggta aacacgtttg
     1561 gcattgatcg caggcatacc atcaagttcg gcctgtctgc gaagcagcgc ccatacccga
     1621 cgataaccat acgtgggcag ctctccgata acatggtgta tacggagaag cacatccgta
     1681 tcatctgagt gacggctgcg gcgaccatct ttccagtcat cggttcgtct gagaattacg
     1741 tgcaactgcg cacgcgacac ccggagacaa cggctgacta agcttactcc ccatccccgg
     1801 gcaataaggg cgcgtgcgct atccactttt ttgcacgccc gtattcaacg gcttctttaa
     1861 ggagttcatt ttccatcgtt tttttgccga gcaggcgctg gagttcttta atctgcttca
     1921 tggcaacagc aagttcagag gcaggaacga cctgctctcc tgcggccaca gcagtaagac
     1981 ttccctcctg gtattgcttg cgccagagaa ataactggct ggctgccaca ccgtgttgcc
     2041 gggcaacaag ggagaccgtc attcccggtt caaaactctg ctgaacaata gcgatctttt
     2101 cctgtgtagt acgccgtctg cgtttctccg gtcctaagac atcaatcatc tgctctccaa
     2161 tgactagtct aaaaactagt attaagacta tcacttattt aagtgatatt ggttgtctgg
     2221 agattcaggg ggccagtcta aagattataa tcctcacact aactgttaaa gctgggactg
     2281 ggcagcattg actactcagg gaaggtactc atctttatct tgtgtttctt acgtaacaac
     2341 cagactaaag tgatttatga acgataacgt tttgctcata ggagcttccg gattcgtagg
     2401 aacccgacta cttgaaacgg caattgctga ctttaatatc aagaacctgg acaaacagca
     2461 aagccacttt tatccagaaa tcacacagat tggcgatgtt cgcgatcaac aggcactcga
     2521 ccaggcgtta gtcggttttg acactgttgt gctgctggca gcggaacacc gcgatgacgt
     2581 cagccctact tctctctatt atgatgtcaa cgttcagggc actcgcaatg tgctggcggc
     2641 catggaaaaa aatggcgtta aaaatattat ctttaccagt tcagttgctg tttatggttt
     2701 gaacaaacac aaccctgacg aaaaccatcc acacgatcct ttcaaccact acggcaaaag
     2761 taagtggcag gcagaggaag tactgcgtga atggtataac aaagcaccaa cggaacgttc
     2821 attaaccatc atccgtccta ccgttatctt cggtgaacgc aaccgcggta atgtctataa
     2881 cttgctgaaa cagatcgctg gcggcaagtt tatgatggtg ggcgcaggga ctaactataa
     2941 gtctatggct tatgttggaa acattgttga gtttatcaag tacaaactga agaatgttgc
     3001 cgcaggttat gaggtttata actacgttga taagccagac ctgaacatga accagttggt
     3061 tgctgaagtt gaacaaagcc tgaacaaaaa gatcccttct atgcacttgc cttacccact
     3121 aggaatgctg ggtggatatt gctttgatat cctgagcaaa attacgggca aaaaatacgc
     3181 tgtcagctct gtgcgcgtga aaaaattctg cgcaacaaca cagtttgacg caacgaaagt
     3241 gcattcttca ggttttgtgg caccgtatac gctgtcgcaa ggtctggatc gaactctgca
     3301 gtatgaattc gtccatgcca aaaaagacga cataacgttt gtttctgagt aatgcagacg
     3361 agtcagagaa aaaagaacca ggttggacta cgattattcg gaaagaggta ctgattaaat
     3421 tatcagtatg tggatttccg agtaatccga gtaacccaga tgctgatggt gtgtagtgtt
     3481 atcgtaaggt ttaatcaaag ttatgcggag ctaagctttg gtatatagcc ataacgtttg
     3541 gctaacctat ggcaataatt tacgcactat cagccactta gaggtaggaa aagaatgacg
     3601 aatttaaaag cagttatacc ggtagcaggt cttgggatgc atatgttgcc tgccactaag
     3661 gcgataccca aagagatgct accgatcgtc gacaagccaa tgattcagta cattgttgac
     3721 gagattgtgg ctgcagggat caaagaaatc ctcctggtga ctcatgcgtc caagaacgcg
     3781 gtcgaaaacc acttcgacac ctcttatgaa ttagaatctc tccttgaact gcgcgtgaag
     3841 cgtcaactgc tggcggaagt acagtccatc tgtccgccgg gcgtgaccat tatgaacgtg
     3901 cgtcagggcg aacctttagg tttaggccac tctattttgt gtgcacgacc cgccattggt
     3961 gacaatccat ttgtcgtggt actgccggac gttgttatcg atgatgccag cgccgacccg
     4021 ctgcgttaca accttgccgc tatgattgcg cgcttcaacg aaacgggacg cagccaggtg
     4081 ctggcaaaac gtatgccggg tgacctctcc gaatactccg tcatccagac caaagagccg
     4141 ctggaccgtg agggtaaagt cagccgcatt gttgaattta tcgaaaaacc ggatcagccg
     4201 cagacgctgg actcagacat catggccgta ggtcgctatg tgctttctgc cgatatttgg
     4261 ccggaactgg aacgtactca gcctggtgca tggggacgta ttcagctgac tgatgctatt
     4321 gccgagctgg cgaaaaaaca atccgttgat gcaatgctga tgaccggcga cagttacgac
     4381 tgcggcaaaa aaatgggcta tatgcaggcg tttgtgaagt atggcctacg caacctgaaa
     4441 gaagggacga agttccgtaa aggtattgag aagctgttaa gcgaataatg aaaatctgac
     4501 cggatgtaac ggttgataag aaaattataa cggcagtgaa gatttgcagc aaaagtaatt
     4561 tgttgcgaat attcctgccg ttgttttata taaaccatca gaataacaac gagttagcag
     4621 tagggtttta gtcaaagttt tccaggattt tccttgtttc cagagcggat tggtaagaca
     4681 attagcgttt gaatttttcg ggtttggcgc gagtgggtaa agctcgtcac atcgtagaca
     4741 tgcatgcagt gctctggtag ctgtaaagcc aggggcggta gcgtgcatta atacctctat
     4801 taatcaaact gagagccgct tatttcacag catgctctga agtaatatgg aataaattaa
     4861 gtgaaaatac ttgttactgg tggcgcagga tttattggtt ctgctgtagt tcgtcacatt
     4921 ataaataata cgcaggatag tgttgttaat gtcgataaat taacgtacgc cggaaacctg
     4981 gaatcacttg ctgatgtttc tgattccgaa cgctatgttt ttgaacatgc ggatatttgc
     5041 gatgcagctg taatggcacg gatttttgct cagcatcagc cggatgcagt gatgcacctg
     5101 gctgctgaaa gccatgttga ccgttcaatt acaggtcctg cggcatttat tgaaaccaat
     5161 attgttggta cttatgtcct tttggaagcc gctcgcaatt actggtctgc tcttgatggc
     5221 gacaagaaaa atagcttccg ttttcatcat atttctactg acgaagtcta tggtgatttg
     5281 cctcatcctg acgaggtaaa taatacagaa gaattaccct tatttactga gacaacagct
     5341 tacgcgccaa gcagccctta ttccgcatcc aaagcatcca gcgatcattt agtccgcgcg
     5401 tggaaacgta cctatggttt accgaccatt gtgactaatt gctctaacaa ttatggtcct
     5461 tatcatttcc cggaaaaatt gattccattg gttattctca atgctctgga aggtaaagca
     5521 ttacctattt atggtaaagg ggatcaaatt cgcgactggt tgtatgttga agatcatgcg
     5581 cgtgcgttat ataccgtcgt aaccgaaggt aaagcggatg aaacttataa cattggtggg
     5641 cacaacgaaa agaaaaacat tgatgtagtg ctcactattt gtgatttgtt ggatgagatt
     5701 gtcccgaaag agaaatctta tcgtgagcaa atcacttatg ttgccgatcg tccgggacac
     5761 gatcgccgtt atgcgattga tgctgagaag attggtcgcg aattgggatg gaaaccacag
     5821 gaaacgtttg agagtgggat tcgtaaaacg gtggaatggt acctgtccaa tacaaaatgg
     5881 gttgataatg tgaaaagtgg tgcctatcaa tcgtggattg aacagaacta tgagggccgc
     5941 cagtaatgaa tatcctcctt tttggcaaaa cagggcaggt aggttgggaa ctacagcgtg
     6001 ctctggcacc tctgggtaat ttgattgctc ttgatgttca ctccactgat tactgtggtg
     6061 attttagtaa tcctgaaggt gtcgctgaaa ccgtaagaag cattcggcct gatattattg
     6121 tcaacgcagc cgctcacacc gcagtagaca aagcagaatc agaaccagag tttgcacaat
     6181 tacttaacgc gacgggtgtc gaagcgatcg cgaaagcagc caatgaagtc ggtgcttggg
     6241 ttattcacta ctctactgac tacgtatttc cggggactgg tgaaatacca tggcaggagg
     6301 cggatgcaac cgcaccgcta aatgtttacg gtgaaactaa gttagctgga gaaaaagcat
     6361 tacaagagca ttgtgcgaag caccttattt tccggaccag ctgggtctat gcaggtaaag
     6421 gaaataactt cgccaaaaca atgttgcgtc tggcaaaaga gcgtgaagaa ttagccgtta
     6481 ttaatgatca gtttggtgcg ccaactggtg cagagttact ggctgattgt acggcacatg
     6541 ccattcgtgt ggcactgatt aaaccggaag tcgcaggctt gtaccatctg gtagctagtg
     6601 gtaccacaac gtggcacgat tatgctgcgc tggtttttga agaggcacgc aaagcaggca
     6661 ttccccttgc actcaacaag ctcaacgcag taccaacaac agcctatcct acaccagctc
     6721 gtcgtccaca taactctcgc cttaatacag aaaaatttca gcagaacttt gcgcttgtct
     6781 tgcctgactg gcaggttggt gtgaaacgaa tgcttaacga attatttacg acaacagcaa
     6841 tttaatagtt tttgcatctt gttcgtgatg gtggagcaag atgtattaaa aggaatgatg
     6901 aaatgaaaac gcgtaaaggt attattttgg cgggtggttc tggtactcgt ctttatcctg
     6961 tgactatggc agtcagtaaa cagctgttac cgatttatga taaaccgatg atctattatc
     7021 cgctctctac actgatgttg gcgggtattc gcgatatttt gattattagt acgccacagg
     7081 atactcctcg ttttcaacaa ctgctgggtg acggtagcca gtgggggcta aatcttcagt
     7141 acaaagtgca accgagtccg gatggtcttg cgcaggcgtt tattatcggt gaagagttta
     7201 ttggtggtga tgattgtgct ttggtacttg gtgataatat cttctacggt cacgacctgc
     7261 ctaagttaat ggatgccgct gttaacaaag aaagtggtgc aacggtattt gcctatcacg
     7321 ttaatgatcc tgaacgctac ggtgtcgttg agtttgataa aaacggtacg gcaataagcc
     7381 tggaagaaaa accgttacaa ccaaaaagta attatgcggt aactgggctt tatttctatg
     7441 ataacgacgt tgtcgaaatg gcgaaaaacc ttaagccttc tgcccgtggt gaactagaaa
     7501 ttaccgatat taaccgtatt tatatggaac aggggcgttt atctgttgcc atgatgggac
     7561 gtggttatgc atggttggac acggggacac atcaaagtct tattgaggca agcaatttca
     7621 tcgcgacaat agaagaacga caggggctga aagtttcctg cccggaagaa attgcttacc
     7681 gtaaagggtt tatcgatgct gagcaggtga aagtattagc tgaaccgttg aagaaaaatg
     7741 cttatggcca gtacctgcta aaaatgatta aaggttatta ataaaatgaa cgtaattaaa
     7801 acagagattc cagacgtatt aattttcgaa ccgaaagttt ttggtgatga gcgtggtttc
     7861 tttatggaaa gctttaatca gaaagttttc gaagaagctg taggacgtaa ggttgaattt
     7921 gttcaggata accattcgaa gtctagtaaa ggtgttttac gcgggctgca ttatcagtta
     7981 gaaccgtatg cgcaagggaa attggtacgt tgcgctgttg gtgaggtttt tgatgtagct
     8041 gttgatattc gtaaatcgtc gcctaccttt ggtaaatggg ttggggtgaa tttatctgct
     8101 gagaataaac ggcaattgtg gatccctgag ggatttgcac atggtttttt agtgctgagt
     8161 gagactgcgg aatttttata taaaacgacg aattattatc atcctgagag tgatagaggg
     8221 attatttgga atgatcccaa tataggtatt gtttggcctg aaagagaaaa tatagtttta
     8281 tcgaaaaagg atataaatca acctaattta tcatatagct tctctctcaa acagttgttg
     8341 tcgtaagaga aaagatctat gtcccttcta agcaatgcaa aatgggtaag cttatctcaa
     8401 tttggacgta ttattataca attgattagc ttgactgttt taacaagatt tataccacca
     8461 gcagattatg gattaatggc tttaggcaca gttgttatga attttgtctt cataatacgt
     8521 gatcttggaa ctgcatcggc cataatacaa agaaaagaag tagatgacga tattttaaat
     8581 tcagtttttt ggttgaatgt aactatggga gtggggttat gcatactaat tatactatgt
     8641 actccactaa ttgagctgtt atttaagaat gagaagttag ataatattct gtatttgtta
     8701 tgcttttgct ttccattagc tagtattggt tctgctcatc aggcattaat ggaaagaaac
     8761 tctcatttta aaaaaatagc aatcatagaa ttgctctcat ctctttctgg ttttgttgtt
     8821 gcattgctag tagcgatcaa taatggaggt gtttatgcat tagtatacca attaatttgt
     8881 tcatcattta tttctagtat attattcttt tatctatcct cttggacgcc atccttgcat
     8941 tttaaatttg agggggttaa aaaaattata ggttttagta gcaggttaac tgtttttaat
     9001 ttagttaact atttctcacg taatgcagat gctatgataa taagtagaaa gtatgatgct
     9061 aatattctcg gcgcttactc tctagcttat agaattatgt tgtttccgct ccagagttta
     9121 acatttattg cgtcaagatc actatatcca atattaagta gaaaccaaga tgatttgaaa
     9181 cttattaggg agatatattt aaaaacaata tttataattt caatgattac cataccatta
     9241 atgttaggat tagtattttt aaggacgccg ttcattacaa tagctttagg gcctaactgg
     9301 gcaataactt ctaatatttt actttggttg gcaccaactg gaattattca gtcaattctt
     9361 agttgctccg gtagcatatt aatgtcaact aataacacga aaacattgat gttattaggt
     9421 atatttgggg ccgcgataca agtattatca ttcttcattg gttctttatt tagtataaat
     9481 atttttgttt ttgcttactt tatagcaaat gtaatcaatg cgctagttgt tttatatttt
     9541 acgtttaaaa gtataaagct taatatttat tcattctgta agatcctact gatcccaacc
     9601 ttatcttgtt cggtgatgat aatagcgctt catttcgtta aaaaaatcat ttacattaat
     9661 aatgtggtaa atgatgtgag ctatttaata attcaggtaa tcatcggttg tattgtatac
     9721 tttattgttt tttatatctt cgtaaatata tgtcttcgat ttaaaatcat gtcagaaaat
     9781 atgatcccag attttctgtt gagaataaag ttctttagcc ataagagatt tttaaattga
     9841 ataaacaatt atttttgatt gttgtttata ataagaaaat agctgagagt tgtactttta
     9901 ataaggttgt taactctaag tttagtgatg attcattatg tgttatatgg aacaatggac
     9961 ccacaagttg tgaggaggag tttttgaatg tatatgagta taataaaaac attcgctatc
    10021 atgaaacatt gcataatatc tcattggcca agatttataa cgaggtaatt aataaatata
    10081 acgctcagat ttatatcatc ctagatgatg attcttcatt aactgatgca tattttgaag
    10141 ctgttaaaca aatatcacct aatgaagtag gtatgcctgt catatattat gatagaaaaa
    10201 taattaaccc atgcataaat ggtcagattt atagtaaagg aattaatatt gatgctaatg
    10261 ataccataac aactatcggg agtggattgg ttgtgggtag ggatgtagca tctcaattat
    10321 tcaaagcata taattctgta tttgatgaac gtttttatct atatggagta gatactacgt
    10381 tttgtttccg acttaataaa ttgaaaataa atgataaaat taagattata gatggctttg
    10441 agcacggcct ttcaaggctt gaagggaaaa atgataaatt aactcttttt agaaaaatag
    10501 agagaagtaa tgatctcgca ttacagatcc gatattataa agaaaaaaaa cattggtttt
    10561 taatgatggt gctgttactc gcgagttcaa ttaagaagtt tattactttt cagccacaac
    10621 aatataaata ttctactatt attaaaacat ttgttaaggg aaagcatgaa cgtcagagat
    10681 aatgataacg caatcaagtt attggagttt gttttaattt tctctttttt tattattgca
    10741 ttatttttct tatattcccc atcttatctt atttacattg tagttagtgc gtttgttttg
    10801 attaagttca ataataacta tagttttggt ttgtttatat tattgataat ggtatggctg
    10861 attgtcaatt ctactaatgt catttcatac caaaattttg tgctcgcgag tgatgatttc
    10921 tcttcttatt ataataacta tcttggattt cttcattacg ggataaatga agaaaatctt
    10981 tttgaatttg gctattcaga aataatgttg cctttgttga actatattgc aagtttaatt
    11041 attgggcaac cagaaccata taagttaaag tttctgtatt ctgtattgca aagcactggt
    11101 gtattatatt gctgcgtaaa aatatctcgt cactataaac ttgatttgag taatagtata
    11161 ttattaacag ctgccgtatt ggtgttttat aagcctttag taggtattca actatctaga
    11221 caaatgttta gctctatttt tattatagct tttattttca cgaataataa atttgagcga
    11281 attatctttt cattaatggc cttttgtttt cattcatcag cgataatatt attaccaata
    11341 acgcaatatc ttctgacaaa aaggaaaaga aaggacttct attatagttt aattatcgtt
    11401 ctgcttgcat atgctgcatt tattgttgtg tttacatcgt ttaaaaatga attaataaat
    11461 attcctattt taaataaatt agactacact tttcgtatgt ttaatgaacc agaaaaaata
    11521 aagcaatctg tatttacatc ttttctcttg ttaatatatt taatccctgc gctgattttt
    11581 tccatcttaa ataaaaaatc tgatggattt aaatataatt tagctatcat ttgtgtattt
    11641 ttaatatctt tttgtattgt acctggtttc aataacagat tattctttgc tgagttatat
    11701 gtctttcttg gtttttttta ttatatggtg tttttttaca atagagtgaa tgcaagagag
    11761 catgtatcga gattaatcat ctttattctt tttactattc tattttttca atctcaattt
    11821 tcatcagaaa ataggcagtt tgatagcttt gaactagaac ctttctatta tcttaaatat
    11881 ttagaatata gagagacaga aataaacaga ggactattag agtaacatgc ctaatttagt
    11941 tatttatact gctgttactg ggcattatga tgaactgggg aatgtttatg aggaaccttc
    12001 agttgattat atctgcttta cagactacaa ctttaccggt acggtaccta aaccgtggaa
    12061 gcaaattaaa ttgccgcctt caaatttctc tgataaagac ttagcaagat attgtaagct
    12121 taatcctcat gtcctattgc ctcagtatca gcattcgttg tggattgatg gcaatattcg
    12181 aataaaaggt aaaatacgaa attttattat tgacattttg agcaagcatc gaattgcagc
    12241 atatgaacat tggtggcgag acaaaacaga acaggaattt catgaatgtg ctagaagtgg
    12301 tttcgatcct gcttggaaat tatataaaca aattgagcgc tataagcatg aaggatacac
    12361 ctcctcagat ttttttgaga ataatatact catgcgtaac catatggaaa gtgacataat
    12421 caaaatgcat gaaatatggt ggggggagta tatttcagga ggtaagcgtg atcaatattc
    12481 ttttacttat gctgcttata aaagtgatgt aaatatatat tcattagggg ttcatgatgc
    12541 tagagttata aaacaatatt ttgactatgt gacacactcc aagaaaaggc cgttaaaaca
    12601 atatatctgg attataataa atagaatcta tatttctcta acgagatgga gcgtaaatga
    12661 accatctcgg acaaataaat tagtgctaaa taatgaaaga gattcaaatg aaaaataaag
    12721 ttgctggagt cattgttaca tataatccag aaattaaaat acttctcagt gcattggagt
    12781 catcgacaaa acagttagac tatatttatt tgatagataa ttgctcatta aataaaaatg
    12841 aggtggctga tttagctgga agatttggga atgtaaaatt tcaggcgctc tctaaaaata
    12901 ttggattagc cagcgcccaa aacatgggca tcaatactgc taaaaacaat aacctaatag
    12961 aatatgtagt tttattcgat caggatagca taatagagaa caattttatt gcttcattac
    13021 ttgatgatta taaaaaagtt ttagcaaagg attctaagat aggagctata ggcccggttt
    13081 tttatgatcc atctactaaa attaattatc ctgcaacagt atatataggt ccttttataa
    13141 aaaaaatatc tataaaggat gagccacaat tcgcaacgta tttaattgcc tctggctgtt
    13201 taataaaaat agatactttg tcttcggttg gattaatgca ggatgatttg ttcatcgatt
    13261 acatcgatgt tgaatggagt ttaagagcaa agagtaaagg ttataattta tatatatcac
    13321 caaacgcaaa aatggcccat acaattggag acgcgaggcg taaatttttg gggcggacaa
    13381 tatctattca ctcccctttt agacgttact tcttggttag aaatagtttt tacatgataa
    13441 ggttaaatta tattccttta gggtacaaga ctagagagtg tatttttaat attgccagga
    13501 tattaacatc tatcatatat tccgatcaaa aaattaaaac aataaaatcg gctttttatg
    13561 gagtcattga tggaattgca ggtcgatttg gccctctaaa acgtaaaata taaagagaaa
    13621 tttcatgaaa attttatata taatcacaaa agctgatgaa atcggtgggg cacaaattca
    13681 tgtgagagac cttgctcgtc gtttacacaa tgatggtaat aaagttacag taatcgtagg
    13741 agaggatggc gctttagtta gacaacttaa agagcttcac atttcggtgc gaatagtaga
    13801 aaatttagtt aggcaaattt caccactaaa tgatataaaa gccattttta acatcagaaa
    13861 gataattaaa gataattcac cggatattat tggtcttcac tcctctaagg ctggaattgt
    13921 tggtcgacta gcagcttgtc gattaaatat tccagtagta tttaccgctc acggttgggc
    13981 atttgcagag ggtatcagtg acaaaaaaag gaaattattt attttcattg agaaaatact
    14041 atctcctttt ttggataaga taattactgt ttcggagcaa gataaaaagt tggcgctaga
    14101 tttaggcgtt gcatcctctg aaaaacaagt cgttattcat aacggaatgc ctgatattac
    14161 tattaagcgt aaggattatt cggattcaac taaagttcga ttaatcagtg tagctcgttt
    14221 ttcagagcaa aaagatcatg aaactttatt tcacgcactc aagttaattt ctacagataa
    14281 ctggacacta actctggttg ggaaaggtcc taagttagaa aatattaaaa aacttgcatc
    14341 tgatttgaat attgcaaata atattctctt tcttggggaa agagatgatg tcgataattt
    14401 attaagtgaa agtgatgttt tctgtttaat aacaaactgg gagggattgc cattaagtat
    14461 tatcgaggct atgcgtgcgt ccttgccagt ggtagctaca gatgttggag gtgtctctga
    14521 gttagtagag cataaaaaaa ccggcttgct tcattctcct aaaaacatac aagaacttgc
    14581 taatatcctg gatactatta tcaatgatag tgacttgaga tataaattgg gaaatgctgg
    14641 aagagttaaa taccaaaata atttcacatt tgaacatatg tattatgcaa caatagatga
    14701 atataataaa ttgatacata ctaaatagtt aattatattt ttcttgtgtt cgctcttttc
    14761 taacagtttt aatttcttta attttaagag ataaatatat tagacaagtt attccaatat
    14821 gagtaaattg gcataacttg taactttaga ccgcaacccg cagtaacccc tgacaggagt
    14881 aaataatgtc aaagcaacag atcggcgtcg tcggtatggc agtgatgggg cgcaaccttg
    14941 cgctcaatat cgaaagccgt ggttataccg tctctatttt caaccgttcc cgtgaaaaga
    15001 ccgaagaagt gattgccgaa aatccaggca agaaactggt tccttactat acggtgaaag
    15061 agtttgttga atctctggaa acgcctcgtc gcatcctgtt aatggtgaaa gcaggtgcag
    15121 gcacggatgc tgctattgat tccctcaagc catacctcga taaaggtgac atcatcattg
    15181 atggtggtaa taccttcttc caggacacca ttcgtcgtaa ccgtgagctt tctgccgaag
    15241 gctttaactt cattggtacc ggtgtctccg gtggtgaaga aggcgcgctg aaaggtcctt
    15301 ctattatgcc tggtgggcag aaagaagcct atgaactggt tgcgccgatc ctgaccaaaa
    15361 tcgccgcagt ggctgaagac ggtgagccat gtgttaccta tattggtgcc gatggcgcag
    15421 gtcactatgt gaagatggtt cataacggta ttgaatacgg cgatatgcag ctgattgctg
    15481 aagcctattc tctacttaaa ggtggtctga acctcaccaa cgaagaactg gcgcagacct
    15541 ttaccgagtg gaataacggt gaactgagca gctacctgat cgacattacc aaagacatct
    15601 tcaccaaaaa agatgaagac ggtaactacc tggttgatgt gatcctggat gaagcggcta
    15661 acaaaggtac cggtaaatgg accagccaga gcgcgctgga tctcggcgaa ccgctgtcgc
    15721 tgattaccga gtctgtgttt gcacgttata tctcgtctct gaaagatcag cgcgttgccg
    15781 cgtctaaagt tctctctggc ccgcaagcgc agccagtagg cgacaaagct gagtttatcg
    15841 agaaagttcg tcgtgcgctg tatctgggca aaatcgtttc ttacgctcag ggcttctctc
    15901 agctgcgtgc ggcgtctgaa gagtacaact gggatctgaa ctacggcgaa atagcgaaga
    15961 ttttccgtgc tggctgcatc attcgtgcgc agttcctgca gaaaatcacc gatgcttatg
    16021 ccgaaaatcc gcagatcgct aacctgctgc tggctcctta cttcaagcaa attgccgatg
    16081 actaccagca ggcgctgcgc gatgtcgtcg cttatgcggt acagaacggt atcccggttc
    16141 cgaccttcgc cgctgcggtt gcctattatg acagctaccg tgccgctgtt ctgcctgcga
    16201 acctgatcca ggcacagcgt gactatttcg gcgcgcatac ttataagcgc attgataaag
    16261 aaggtgtgtt ccataccgaa tggctggatt aatctgattt aaatcaatta atcaatgcaa
    16321 ggcccggaga aaccctccgg gcttttttat tatacaaagc ggcaggttat ggcctttttt
    16381 tataatttat ggttaaaaaa gcgatataat acagcgccgc acagcaggat cgctgccttg
    16441 acagttcatc tacatcagcg ttaaaaatcc cgcagtagat gaagttgtgg tgatggatta
    16501 atgaccactc taaatgttta accggaagaa gtcagagcta atgaaaataa caatttcagg
    16561 aacaggttat gttggtcttt caaatggtat tctgattgcg caaaaccacg aagtggttgc
    16621 actggatatc gttcaggcca aagtggacat gcttaacaag aagcagtcac cgattgttga
    16681 taaggagatt gaagagtatc tggcgactaa agatctcaat ttccgcgcta cgacagataa
    16741 gtatgacgcg tataaaaatg ccgattacgt tattattgcc actcctaccg attatgaccc
    16801 gaaaacaaat tattttaata cctcaagtgt cgaagcggtc atccgtgatg tgacagaaat
    16861 taatcccaac gcggtaatgg tgattaaatc tacaattccg gtaggtttca ccaaatcaat
    16921 taaagaacgt ctgggtatcg ataaccttat tttctctccg gaatttctcc gtgaaggtaa
    16981 agctctttac gataatctcc atccttcacg tattgtcatt ggcgagcgtt cagaacgcgc
    17041 agaacgtttt gctgcgttgt tacaggaagg cgcgattaag caaaatatcc cgaccctgtt
    17101 taccgactcc actgaagcag aagcgattaa actgtttgct aatacctatc tggcgatgcg
    17161 cgtagcatac tttaatgaac tggatagcta tgcagaaagt ttaggtctga atactcgtca
    17221 gattatcgaa ggtgtttgtc tcgatccgcg tattggcaac cattacaaca atccgtcgtt
    17281 tggctatggt ggttattgtc tgccgaaaga taccaagcag ttactggcga actaccagtc
    17341 tgtgccgaat aacctgatct cggcaattgt cgatgcaaac cgcacgcgta aagactttat
    17401 tgccgatgcc attttgtcac gcaagccgca agtggtgggt atttatcgtc tgattatgaa
    17461 gagcggttca gataacttcc gcgcgtcttc cattcagggg attatgaaac gtattaaggc
    17521 gaaaggcgtt gaagtgatca tctacgagcc ggtgatgaaa gaagactcat tcttcaactc
    17581 tcgtctggaa cgtgatctcg ccaccttcaa acaacaagcc gacgtcatta tttccaaccg
    17641 tatggcagaa gagcttaagg atgtggcaga taaagtctac acccgcgatc tctttggcag
    17701 cgactaacat cctgttatca gggcgatttt cgccctgatt gtcttatgtt ccctttgtaa
    17761 taattcatta tttttatcat ttatcctata gcattcatgg cgattatcgc taaactatgg
    17821 cgacttaaaa ttattttgtc cgttagggta atgatgagag tagaaaataa taatgtttct
    17881 gggcaaaacc atgacccgga acagattgat ttgattgatt tactggtcca actatggcgt
    17941 ggcaagatga ctatcatcat ttctgtcatt gtggctattg ccctggctat agggtatctg
    18001 gccgttgcca aagaaaagtg gacttcaaca gctatcgtta ctcaacctga tgtgggacaa
    18061 atcgctggtt ataacaatgc cataaatgtg atttatggtt cggcagcgcc gaaagtgtct
    18121 gagatccagt cgatgttaat tggtcgtttc agcacgacat tttctgcatt agcagaaacg
    18181 ctggataatc aggaagaacc tgaaaaactg accattgagc ctaccgttaa aaatcagtca
    18241 ttacctttag cggtttctta tgttggtgaa acacctgaag gtgcgcagaa acagctggcg
    18301 aagtatatcc agcaagttga cgaccaggta aatgaagaac tggaacaaga cctgaaagac
    18361 aacatcgcgc tgcaaatgaa aaacttgcag gattctctga agacgcagga agtggtcgct
    18421 caagaacaga aagatctgcg tatccgtcag attcaggaag cgttgcagta tgcgaatcag
    18481 gcgcaggtga caaagccgca gattcaacag acacaggacg taacccagga tactatgttc
    18541 cttttgggga gcgaagcgct ggagtcgatg attaagcacg aagcgacccg tccgttggtg
    18601 ttctcatcaa actactatca gactcgtcaa aacctgctgg atatcgacaa tcttgacgtt
    18661 gataaacttg atattcatgc ttaccgctat gtaatgaaac cgacgttacc tattcgtcgc
    18721 gatagcccga aaaaggcaat taccttgatt ctggcagtgc tgctgggcgg catggttggc
    18781 gcggggattg tgcttgggcg taacgcgctg cgtaattaca acgcgaaata atattattgt
    18841 gcatttaaga gaaacgggca gggtggtgac accatgcccg ttttttttgc cggatgcgat
    18901 gctggcgcat cttatccggc ctacgtgtgt tgagataatg tgtaggcacg ataagcttgc
    18961 gcatcgggca atggctccgg atgcgacaac aacatcgcat ccgctgacct cgcaactcac
    19021 tgatgccgtt tacgcaggtt ctcaatcacc gttgttaaat ccagatcctg atcctgcaac
    19081 aacaccagca ggtgatacat caaatcagac gcctcgttgg tcagttcaaa gcggtcatgt
    19141 accgttgctg ccagtgcggt ttccacgcct tcttcgccca ctttctgcgc aatgcgttta
    19201 gtgccgctgg catacagttt ggcggtgtag gaggtttccg ggtcggcaga tttacgttcg
    19261 gcgagcagtt gttccagttg atgcaggaac agccactggt gagcggtatc gccgaagcag
    19321 ctgctggtgc ctttgtggca ggttggcccg atgggattcg ccagcaccag taacgtgtcg
    19381 ttgtcgcagt ccggcgcaat atttactacg ttgaggaaat tacctgacgt ttcgcctttg
    19441 gtccacagtc gctgtttagt gcgcgagaag aaggtgactt tgccgctttc gatggttttg
    19501 tctaaggctt ccgggttcat atagcccagc attagcactt cgccagatac cgcgtgttgc
    19561 acaatcaccg gcatcagacc gtcggttttt tcccagtcca gttcgcgacg ttgttgttct
    19621 gttaacatat cctgatctcc acgccctgtg ttgccaggta cgcttttaat tcaccaatat
    19681 tgattatttg tttgtggaat acggaagctg ccagcgcacc gtcaacgtcg gcatcgcgga
    19741 aggcttccag gaagtgttcc atggtgcccg cgccaccgga ggcaatcagc gggacgtggc
    19801 aaacttcacg cacttttttc agctgttcga ggtcgtaacc gttacgcacg ccgtcctgat
    19861 tcatcatgtt gaggacgatt tctccggcac cgcgtttttg cacttcctgc acccagtcga
    19921 gtgt
//