<pre>LOCUS       KJ755558               10991 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain Bi 626-42 serotype O12:K5:H- O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ755558
VERSION     KJ755558.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10991)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10991)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Liu,Y. and Debroy,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Bi 626-42"
                     /serotype="O12:K5:H-"
                     /db_xref="taxon:562"
     misc_feature    1..10991
                     /note="O-antigen gene cluster"
     gene            85..1044
                     /gene="epsH"
     CDS             85..1044
                     /gene="epsH"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="AIG62541.1"
                     /translation="MKSKLVSIIIPAYNAQDWIERCLVSCLNQSYQNIEIIVINDGST
                     DNTANILEKFNVNKLKIFHEKNSGVAIARRKGLDYSRGEYIFFLDSDDFINESALEIL
                     VTKIDNYNADVVIAQAKHLKKKSTFTSSFKIEIDPLYSFLNGSLPRTLWPLLFKRSVI
                     EKNLIPSQYTVSEDYVIMSKIYTLPIKIVVVNDCLYNYVKHTGSVTANITPQKYNDHY
                     KAHHYIEKQLFETLSDKYKISFIRNNLDFLYSLIIINSPYVSHQMEYIWGKYIKEDIN
                     KATRRLSLKKKIILKLNHVALMRLGPLRMIILLMNKLRRYNEN"
     gene            1156..2376
                     /gene="wzx"
     CDS             1156..2376
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62542.1"
                     /translation="MQMCNYAIPLITLPYLTRCLGFYQYGVLNTAINIILYIILVIDF
                     GFNLSATREISQKKNNFVAVSKIFTDVIFAKIILFFGVLVFTFLILKLIPDYQDVTRL
                     VYFMLPQVLSSILFPLWLYQGYEKIGYAAFINVLGKCITIPLLLIFVQNSKDVTNAAI
                     ILSWSSLVPLLIYYIRSNLPRTKFYTSRCRFKYLNRTLANALVVFIGSIAVNLYTLST
                     PLVLSIVSDFDQVGYFVAADKIRAAFIGVFLIVGQAIYPRASALYNEDIIAYNKFVIS
                     IIFYQIIVCTFSAIAFYYFMPEVAPIILSQNKLNVEELSVIIKLMSPMIILIPLSVIL
                     ANCVLLPQRKNKQYALVPIITVIIHMSYSIILCEKLGALGASYAILLTEIISCALLAY
                     FTFKDRLIQLNPAR"
     gene            2389..3492
                     /gene="wzy"
     CDS             2389..3492
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG62543.1"
                     /translation="MNHSYIYVYIFFVFLSFFELNDTNRARCDLYYAIQVIVLLLTMG
                     LSYQIGVDWVEYERVYNGNSESLNNYEFGYIVLNNIANYLGASFWGFVILIKYTFFIS
                     LFLMVRKFSRLPVLTTTLILGLLFPFINDPLRQIISASILFFTIYFYNRPPKILGIIG
                     GGVFHSTFIIIIIRYLKIFTKRSIVLIFVFVGVAILLFLKYGSFLGDGMLFRKLNFYM
                     EYSSLSNLYGFALRFLLLSFIVFNIDVYKANCKFIRGDICYYFWIFSITYLWLEIIFV
                     TFPLIPQRMRLYLLPFAFILLSNYLYYSRLTFTKNILAFICVIISFSSLCLFLDGPMG
                     HFYSITDNIVLKYIQGFTNDKTYDANQFWINGI"
     gene            3493..4590
                     /gene="mshA"
     CDS             3493..4590
                     /gene="mshA"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="AIG62544.1"
                     /translation="MKKNVLIIASAAKEGGALSILTESLNNLKILNIHFTVVVNPSVV
                     KYLPVANNIKYHMVDTSAWIKRIAYDFYGYPGLKYEEYIACINFQNIPIRTKIKQIVY
                     YHQSLPLSDTLFSPFNKETRVLYLYQKFYGLFFKLNKRYISHLVVQANWIREKISEKF
                     GVENNKIEVIAPQVEIFKYISLEFNKIRPVENVFFYPANAYTYKNHLIIIKALNYLGS
                     VYIKKHRIKVVFTINNSESNYLQSMVLNYNLQDVVSFIGRIPRADVYSLLIKSKALLF
                     PSKLETYGLPLKEARQLGVFIVASKLDYATEVLEGYEYKRLCSPDSAKEWASAIKEII
                     ENKIDVGNTVTRDNIKESDLFSDFIKSKIME"
     gene            4597..5631
                     /gene="fnlA"
     CDS             4597..5631
                     /gene="fnlA"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="AIG62545.1"
                     /translation="MFAEKTLLITGGTGSFGNAVLRRFLDTDIKEIRIFSRDEKKQDD
                     MRKKYNNQKIKFYIGDVRDYSSILNASRGVDFIYHAAALKQVPSCEFHPMEAVKTNIL
                     GTENVLEAAIANRVRRIVCLSTDKAVYPINAMGISKAMMEKVIVAKSRNLDSSKTVIC
                     GTRYGNVMASRGSVIPLFVDLIKSGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGNN
                     GDIFVQKAPAATIQTLAIALKELLNAHEHPINIIGTRHGEKLYEALLSREEMIAAEDM
                     GDYYRVPPDLRDLNYGKYVEHGDRRISEVEDYNSHNTERLDVEGMKKLLLKLPFIRAI
                     RSGEDYELDS"
     gene            5633..6739
                     /gene="fnlB"
     CDS             5633..6739
                     /gene="fnlB"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2-acetamido-2,6-dideoxy-beta-L-talose
                     4-dehydrogenase"
                     /protein_id="AIG62546.1"
                     /translation="MKILVTGASGFIGRNLVFRLKEAGYNELITIDRNSSLADLEQGL
                     KQADFIFHLAGVNRPVKESEFEEGNSNVTQQIVDILKKNSKNTPIMLSSSIQAECDNA
                     YGKSKATAEKIIQQYGETTNAKYYIYRLPNVFGKWCRPNYNSFIATFCHRIANDETIT
                     INDPSAVVDLVYIDDFCSDILKLLEGANETGYRTFGPIYSVTVGEVAQLIYRFKESRQ
                     TLITEDVGNGFTRALYSTWLSYLSPEQFAYTVPSYSDDRGVFCEVLKTKNAGQFSFFT
                     AHPGITRGGHYHHSKNEKFIVIRGSACFKFENIVTGERYEFNVSSDDFKIVETVPGWT
                     HDITNNGSDELVVMLWANEIFNRSEPDTIARVLS"
     gene            6736..7866
                     /gene="fnlC"
     CDS             6736..7866
                     /gene="fnlC"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="AIG62547.1"
                     /translation="MKKLKVMSVVGTRPEIIRLSRVLAKLDEYCDHLIIHTGQNYDYE
                     LNEVFFKDLGVRKPDYFLNAAGKNAAETIGQVIIKVDDVLEQEKPEAMLVLGDTNSCI
                     SAIPAKRRKIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINMTYSDIAREYLLAEGV
                     PADRIIKTGSPMFEVLTHYMPQIDGSDVLSRLNLTPGNFFVVSAHREENVDTPKQLAK
                     LANILNTVAERYNIPVVVSTHPRTRNRINENGIQFHKNILLLKPLGFHDYNHLQKNSR
                     AVLSDSGTITEESSIMNFPALNIREAHERPEGFEEGAVMMVGLESERVLQALEIIATQ
                     PRGELRLLRQVSDYSMPNVSDKVVRIIHSYTDYVKRVVWKQY"
     gene            7866..9074
                     /gene="wbuB"
     CDS             7866..9074
                     /gene="wbuB"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="AJE24506.1"
                     /translation="MKLALIIDDYLPHSTRVGAKMFHELGLELLGKGHDVTVITPDIT
                     LQAIYSVSMIDGIKVWRFKSGPLKDIGKAKRAINETLLSFRAWRALKHLIQHDTFDGI
                     VYYSPSIFWGDLVKKIKQRCQCPSYLVLRDMFPQWVIDAGMLKAGSPIEKYFRYFEKK
                     SYQQADRIGLMSDKNLEIFRQANKGYPCEVLRNWASMTPVCASDDYNSLRQKYDLKDK
                     VIFFYGGNIGHAQDMTNLLRLARNMMRYHDAHFLFIGQGDEVDLINSLAAEWNLTNFT
                     HLPSVNQEEFKLILSEVDVGLFSLSSRHSSHNFPGKLLGYMVQSIPILGSVNGGNDLM
                     DVINKHRAGFIHVNGEDDKLFESAQLLLADSALRKQLGQNANVLLKSQFSVESAAHTI
                     EVRLEAGECV"
     gene            9065..9472
                     /gene="wbuC"
     CDS             9065..9472
                     /gene="wbuC"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin fold metalloprotein"
                     /protein_id="AIG62550.1"
                     /translation="MRLVDDNILDELFRTAVNSERLRAHYLLHASHQEKVQRLLIAFV
                     RDSYVEPHWHELPHQWEMFVVMQGQLEVCLYEKNGEIQKQFIVGDGTGISVVEFSPGD
                     IHSVKCLSPNALMLEIKEGPFDPLKAKAFSKWL"
     gene            9585..10991
                     /gene="gnd"
     CDS             9585..10991
                     /gene="gnd"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase,
                     decarboxylating"
                     /protein_id="AIG62551.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNCELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKGEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
ORIGIN      
        1 ctggtagctg taaagccagg ggcggtagcg tgggttaatt ctaaaattag aattttacga
       61 cactataagt aaaataggtt tataatgaag agtaaattag tttccataat cataccagca
      121 tacaatgctc aggattggat tgaaaggtgt ttagtatctt gccttaatca gagctatcaa
      181 aatatagaaa ttattgtaat aaatgatgga agtacagata atactgctaa tattctcgag
      241 aagttcaatg taaataaact gaaaatattt catgaaaaaa actctggtgt agcaatagca
      301 agacgaaaag gtttggatta ttctagagga gagtatatct tttttcttga tagtgatgat
      361 tttataaatg agtctgcttt agaaatattg gtaaccaaga ttgataatta taatgctgac
      421 gtggttatag cgcaagctaa acatcttaaa aagaaaagca cttttacatc atcttttaaa
      481 attgaaattg atccactata ttcatttttg aatggatctt taccaagaac tttatggcca
      541 ttattattta aaagatctgt tattgagaaa aatctaatac catctcaata tacagttagt
      601 gaagactatg taataatgtc aaaaatatat accttgccaa ttaaaatcgt tgtcgttaat
      661 gattgtctat acaattatgt aaaacacacg ggtagcgtaa cagcgaatat aacgccacag
      721 aaatataatg accactataa agctcaccat tatattgaaa aacagttatt cgaaacgtta
      781 tcagataaat ataaaataag ttttataaga aacaatttag attttcttta tagtttaatt
      841 attatcaatt caccatacgt cagtcatcaa atggaatata tttgggggaa atatattaaa
      901 gaagatatta ataaagctac aaggaggctt tctctgaaga aaaaaataat acttaaactt
      961 aatcacgtag ccttgatgag acttgggccg ttaagaatga taatattatt aatgaataaa
     1021 ctaagaagat acaatgagaa ttaaaactag taatataaaa atgataatga taatattttg
     1081 ataatgacat agttaagtca taaaaggtta ttttctgtga atatagtatt gaaaaattca
     1141 atgtatcttg tttttatgca gatgtgcaat tatgctattc ccttaattac gctaccttat
     1201 ttaacaagat gcttggggtt ttatcaatat ggtgtattga atactgccat caatattata
     1261 ctatacatca tcttggttat tgattttgga tttaatttaa gcgccacaag agaaatatca
     1321 cagaaaaaaa ataactttgt agcggtatct aaaatattta cagatgtgat ttttgcaaaa
     1381 attatacttt ttttcggtgt gcttgttttt actttcctca tacttaaact aattcccgac
     1441 tatcaagatg taacaagatt ggtttatttt atgttacctc aagtcctatc ttcgatatta
     1501 ttcccactgt ggttatatca agggtatgaa aaaattggat atgctgcatt tataaacgtt
     1561 cttggaaaat gtataacaat cccattgcta ttaatctttg tacaaaatag caaagatgta
     1621 acaaatgctg caattatatt aagttggtct tctttagttc ccttattaat ttattatata
     1681 cgtagtaatc ttccgcgaac aaagttttat acctcaaggt gtagattcaa gtacttaaat
     1741 agaacattag ctaatgcact ggtcgttttt attggttcca ttgctgtaaa cctgtatact
     1801 ttaagcacac cattagtatt aagtatagtt agtgattttg atcaagttgg ttattttgtt
     1861 gcagctgata aaataagggc tgcatttatt ggcgtttttt taattgtagg gcaggcgatt
     1921 tatcctagag caagtgccct gtataatgaa gatataattg cgtataataa atttgtcata
     1981 agcattatat tttaccaaat tatagtatgt acgttctctg ctatagcctt ttattacttc
     2041 atgccagagg tggcaccaat tattttgagt caaaacaaat taaacgtaga agagttatct
     2101 gttataatta aattgatgtc gccaatgatt attttgatac ctctttcggt gatattagct
     2161 aactgcgtac ttttgcctca aaggaaaaat aaacaatacg ctttagtgcc tattattaca
     2221 gtcataattc atatgagtta ctctataatt ttgtgtgaaa agttaggtgc gctaggggca
     2281 agttacgcaa tacttctcac agaaattatt agttgtgctt tactagcata ttttacattc
     2341 aaagatagac taattcagct caatccagcc agataatatt ggataattat gaatcattct
     2401 tatatctatg tttatatatt ttttgtgttt ctttcatttt ttgagcttaa tgatactaat
     2461 agagcaagat gtgatttata ttatgccata caagttattg tattattact aacaatgggg
     2521 ttgtcgtatc aaataggtgt tgattgggtt gaatatgaac gtgtttataa tgggaattcg
     2581 gaatcattaa ataattacga attcggttat atagtattga ataatattgc aaactattta
     2641 ggggctagtt tttgggggtt cgtgatatta ataaaatata cgttttttat atcattgttt
     2701 cttatggtaa gaaaattcag tagattacct gttctgacta caacattaat actagggttg
     2761 ttatttccat ttattaatga ccctttacga caaataatat ctgcaagtat attgttcttt
     2821 acgatttatt tctataacag gccccctaaa atattgggga ttataggggg tggggtattt
     2881 cattctacat ttattataat tataattcgt tatcttaaaa tttttacaaa aagatctatt
     2941 gtgcttattt tcgtttttgt gggggtggca attcttttgt tcttaaagta tggttctttt
     3001 ttaggtgatg gaatgttatt taggaaatta aatttttaca tggagtattc atcattatca
     3061 aatttgtatg gctttgccct aaggtttttg ctattgtcat ttattgtttt taatattgac
     3121 gtttacaaag ctaattgcaa attcatcaga ggagatatct gttattattt ctggattttt
     3181 agtataacat atttatggct tgaaataata tttgtaacat tccctttaat ccctcagcgg
     3241 atgcggcttt atttgttacc atttgctttt atactattaa gtaactacct ttattatagt
     3301 cgattaactt tcacaaaaaa tattcttgct tttatatgtg taatcatttc attttcttca
     3361 ttgtgtcttt ttttagatgg tcctatgggg catttttata gcattactga taatatagta
     3421 ttaaaatata ttcagggttt tactaacgat aaaacatatg atgcaaacca gttctggatt
     3481 aatggaattt aaatgaaaaa aaatgtcttg ataatagctt ctgcagcaaa agaaggtggg
     3541 gctttaagca tacttacaga aagtttaaat aaccttaaaa tattaaatat tcattttaca
     3601 gttgtggtca atccatctgt ggttaaatat ttgcctgttg ctaataatat aaaatatcat
     3661 atggtagata catcggcgtg gattaaaaga atagcatatg atttctatgg ctatcctggt
     3721 ctcaagtatg aagaatatat agcgtgcata aattttcaaa atatacctat aagaactaaa
     3781 ataaagcaaa tagtatatta tcatcagtca ttacctcttt cggatacatt attctcaccg
     3841 tttaataaag aaacgcgagt tttatattta tatcaaaaat tctatggttt atttttcaag
     3901 ttaaacaaaa ggtatatatc gcatctagta gttcaggcaa attggattag agaaaaaata
     3961 tctgaaaaat ttggtgttga aaataataaa attgaagtta ttgcacctca agtcgaaatt
     4021 tttaaatata taagtttaga gtttaataaa ataagacctg tagagaatgt atttttttat
     4081 cctgcaaatg cgtatactta taaaaatcat ttgattataa tcaaagcttt aaattattta
     4141 gggagtgttt atattaaaaa acatcgtatt aaggttgttt ttactataaa taatagtgag
     4201 tcaaattatc ttcaatcaat ggttttgaat tataatttgc aggatgttgt tagttttatt
     4261 ggtcgaattc cgagagccga tgtttattct ctcttgataa aatcaaaagc tcttcttttc
     4321 ccaagtaaat tagaaactta tggtttacca ttaaaagagg cgcggcaact aggtgtattt
     4381 attgtcgcat cgaagttaga ttacgcaact gaagtattgg agggatacga atataaaaga
     4441 ttatgctcgc cagatagtgc taaagaatgg gcttcggcta taaaggaaat tatagaaaat
     4501 aagattgatg tcggaaatac agtcactaga gataatatta aagagagcga tttgttttct
     4561 gattttatta aaagtaaaat aatggagtga ttatttatgt ttgcagaaaa aacattgctt
     4621 attactggtg gtacggggtc ttttggtaat gctgttctta gacgtttcct tgacactgat
     4681 atcaaagaaa tacgcatttt ttctcgagat gagaaaaagc aggacgatat gaggaaaaag
     4741 tataataatc agaaaattaa gttctatata ggtgatgttc gcgattattc gagtatcctt
     4801 aatgcttctc gaggtgttga ttttatttat catgcagcag ctctgaagca agtgccttcc
     4861 tgcgaattcc accccatgga agctgtaaaa acgaatattt taggtactga aaacgttcta
     4921 gaagctgcaa tagctaatcg cgttaggcga attgtatgtc tgagtacaga taaagccgta
     4981 tatcctatta atgcaatggg catatctaaa gcaatgatgg aaaaagtcat tgttgcaaaa
     5041 tcacgtaatc ttgatagttc aaaaacagtt atctgcggaa cacgttatgg gaatgtaatg
     5101 gcttcacgtg gatcggtcat cccattgttt gttgatctaa tcaaatctgg taaaccattg
     5161 accattaccg atcccaatat gactcgtttc atgatgacgc ttgaggatgc tgttgatctg
     5221 gtcctttatg ccttcgagca tggaaataac ggtgatattt tcgttcagaa agctcctgcg
     5281 gcaacaattc aaacattagc cattgcactt aaggaattgc taaatgccca tgaacatcca
     5341 atcaatatta ttggaactcg acacggggaa aaactttacg aagcgttatt gagccgagag
     5401 gaaatgatag cagcggaaga tatgggtgat tattatcgtg ttccaccaga tctccgcgac
     5461 ttgaactatg gtaaatatgt ggaacatggt gaccgtcgta tctcggaagt ggaagattat
     5521 aactctcata atactgagag attagatgtt gagggtatga aaaaattact gctaaaactt
     5581 ccttttatcc gggcaattcg ttctggtgaa gattatgagt tggattcata atatgaaaat
     5641 tttagttact ggtgcttcag ggtttatcgg ccgtaatttg gttttccgcc ttaaggaggc
     5701 tggttataac gaacttatta cgatagatcg taactcttct ttggcggatt tagagcaggg
     5761 acttaagcag gcagatttca tttttcacct tgcaggagta aatcgtcctg tgaaggagag
     5821 tgaatttgaa gagggaaata gcaacgtaac tcaacagatt gttgatattc tgaaaaaaaa
     5881 tagtaaaaat actcctatca tgctgagttc ttccatccag gctgaatgtg ataacgctta
     5941 tggaaagagt aaagcgactg cggagaaaat cattcagcag tatggggaaa cgacaaatgc
     6001 caaatattat atttatcgtt tgccgaatgt attcggtaag tggtgtcgcc caaattataa
     6061 ctcctttata gcaactttct gccatcgcat tgcaaatgat gaaactatta caattaatga
     6121 tccttcagca gttgttgatc tggtgtatat agatgacttt tgttctgaca tattaaagct
     6181 attggaagga gcgaacgaaa ctggttacag gacattcggt ccaatttatt ctgttactgt
     6241 tggtgaagtg gcacaattaa tttaccgatt taaagaaagt cgccaaacat taatcaccga
     6301 agatgtaggt aatggattta cacgtgcatt gtactcaaca tggttaagtt atctgtctcc
     6361 tgaacagttt gcttatacgg ttccttctta tagtgatgat agaggggtat tctgtgaagt
     6421 attgaaaacg aaaaacgcgg gccagttttc gttctttact gcgcatccag gaattactcg
     6481 gggggggcat tatcatcatt ccaaaaatga gaaatttatt gtcatccgag gaagtgcttg
     6541 tttcaaattt gaaaatattg tcacgggtga gcgatatgaa tttaatgtct cctcagatga
     6601 ttttaaaatt gttgaaacag ttccggggtg gacgcatgac atcactaata atggctcgga
     6661 tgagctagtt gttatgcttt gggcaaatga aatatttaat cgttctgaac cagatactat
     6721 agcgagagtt ttatcgtgaa aaaattgaaa gtcatgtcgg ttgttgggac tcgtccagaa
     6781 attattcgac tctcgcgtgt ccttgcaaaa ttagatgaat attgtgacca ccttattatt
     6841 catactggcc aaaactacga ttatgaattg aatgaagttt ttttcaaaga tttgggtgtt
     6901 cgcaaaccag attattttct taatgccgca ggtaaaaatg cagcagagac tattggacaa
     6961 gttatcatta aagttgatga tgtccttgaa caggaaaaac cagaagctat gttagttctt
     7021 ggcgatacta actcctgtat ttcagcaata ccagcaaagc gtcgaaaaat tccgatcttc
     7081 catatggagg cggggaatcg ttgttttgac caacgcgtac cggaagaaac taacagaaaa
     7141 atagttgacc acactgctga tatcaatatg acatatagcg atatcgctcg tgaatatctt
     7201 ctggctgaag gtgtaccagc cgatagaatt attaaaaccg gtagcccaat gtttgaagta
     7261 ctcactcatt atatgccgca gattgatggt tccgatgtac tttctcgcct gaatttaaca
     7321 cctgggaatt tctttgtggt aagtgcccac agagaagaaa atgttgatac ccctaaacag
     7381 ctcgcgaaac tggcgaatat acttaatacc gtggctgaaa gatataatat cccggtagtt
     7441 gtttctactc atccgcgcac tcgcaaccgc atcaatgaaa acggtattca attccataaa
     7501 aatatcttgc tacttaagcc attaggattt cacgattaca accatctgca aaaaaattca
     7561 cgtgctgttt tatcggacag cgggactatt acagaagagt cctccattat gaatttccct
     7621 gcactcaata tacgagaagc gcacgaacgc ccggaaggct tcgaagaagg ggcagtaatg
     7681 atggttggtc ttgaatctga gcgcgtgtta caggcattag aaattattgc aacacaacct
     7741 cgtggtgaat tacgcttact tcgtcaggtc agtgactata gtatgccaaa tgtttcagat
     7801 aaagttgtgc gtattatcca ttcatatact gactacgtta aacgggttgt ctggaagcaa
     7861 tactaatgaa acttgcatta atcattgatg attatttgcc ccatagcaca cgagttgggg
     7921 ctaaaatgtt tcatgagtta ggcctggaat tgctgggcaa aggccatgat gtaactgtaa
     7981 ttacgcctga catcacttta caagcaatct attctgttag tatgattgat ggtataaagg
     8041 tttggcgttt caaaagtgga cctttaaagg atataggtaa ggctaagcgt gccataaatg
     8101 aaactctttt atcttttcgt gcatggcgtg cattaaagca cctcattcaa catgatacat
     8161 ttgatggtat cgtttattat tccccctcta ttttttgggg agacttggtt aaaaaaataa
     8221 aacagcgatg ccagtgccca agctatctgg tcctaaggga tatgtttcca cagtgggtca
     8281 ttgatgcagg tatgttgaaa gccggttcgc caattgaaaa atatttcagg tattttgaaa
     8341 aaaaatcata tcaacaggct gaccggattg ggttaatgtc tgataagaat cttgagatat
     8401 ttcgtcaggc taataaaggt tatccatgtg aagttttacg taattgggcc tcaatgactc
     8461 ctgtgtgtgc cagcgatgat tataattccc ttcgccaaaa atacgatcta aaagataaag
     8521 tcattttttt ttatggaggt aatattgggc atgctcagga tatgacaaac ttattgcgcc
     8581 ttgcgcgcaa tatgatgcgt tatcatgatg ctcatttcct gtttataggg cagggtgatg
     8641 aagttgacct gattaattct cttgctgcag aatggaattt aactaatttc actcatctac
     8701 cttcagtgaa ccaggaagag tttaaattaa ttttatctga agttgatgtt ggcctgttct
     8761 ccctttcgtc tcgccattct tcacataatt tcccggggaa attactaggg tatatggttc
     8821 aatcaatccc gatccttggg agcgtgaatg gcggtaatga tctgatggat gtaattaata
     8881 agcacagggc cgggttcatt catgttaatg gtgaagatga taaactgttc gaatctgcac
     8941 aattgcttct tgcagattcg gctttaagaa agcagttagg tcagaacgct aatgtgttgt
     9001 taaaatctca attttcggtt gaatcggcgg cacatactat cgaagtccga ctggaggcag
     9061 gagaatgcgt ttagttgatg acaatattct ggatgagctt tttcgcacag cagtaaattc
     9121 tgaacgtttg cgcgctcatt atttattgca cgcatctcat caggagaagg ttcaacgttt
     9181 acttattgca tttgtacgcg acagctatgt tgagccccat tggcatgagt taccgcatca
     9241 gtgggaaatg tttgtcgtca tgcaagggca attagaagtt tgtttgtatg agaaaaatgg
     9301 tgagatacaa aaacagttta ttgttggaga cggtactgga ataagcgtcg tggaattttc
     9361 cccaggtgat atacatagtg tcaaatgcct gtcaccgaat gcccttatgt tggagataaa
     9421 ggaaggacca tttgacccac tgaaagctaa ggctttttct aagtggttat agggcgatac
     9481 accacccttt attcttctat cttattctat acatgctggg ttaccatctt agcttcttca
     9541 agccgcgcaa ccccgcggtg aacaccccct gacaggagta aacaatgtca aagcaacaga
     9601 tcggcgtcgt cggtatggca gtgatggggc gcaaccttgc gctcaacatc gaaagccgtg
     9661 gttataccgt ctctattttc aaccgttccc gtgaaaagac ggaagaagtg attgccgaaa
     9721 atccaggcaa gaaactggtt ccttactata cggtgaaaga gtttgttgaa tctctggaaa
     9781 cgcctcgtcg catcctgtta atggtgaaag caggtgcagg cacggatgct gctattgatt
     9841 ctctcaagcc atacctcgat aaaggtgaca tcatcattga tggtggtaac accttcttcc
     9901 aggacacaat tcgtcgtaac tgtgagctgt ctgcagaagg ttttaacttt atcggtaccg
     9961 gtgtttccgg tggtgaagaa ggtgcgctga aaggaccttc catcatgcct ggtgggcaga
    10021 aagaagccta tgaactggtt gctccgatcc tgaccaaaat cgccgccgtt gctgaagatg
    10081 gcgaaccgtg cgttacctat attggtgccg atggcgcagg tcactatgtg aagatggttc
    10141 acaacggtat tgaatacggt gatatgcagc tgattgctga agcctattct ctgcttaaag
    10201 gtggcctgaa tctctctaac gaagaactgg cgcagacgtt taccgagtgg aataacggtg
    10261 aactaagcag ctacctgatc gacatcacca aagacatctt cactaaaaaa gatgaagacg
    10321 gtaactacct ggttgatgtg atcctggatg aagcggctaa caaaggtacc ggtaaatgga
    10381 ccagccagag cgcgctggat ctcggcgaac cgctgtcgct gattaccgag tctgtgtttg
    10441 cacgttatat ctcttctctg aaagagcagc gtgttgccgc atctaaagtt ctctctggcc
    10501 cgcaagcgca gccagctggc gacaaaggtg agttcatcga aaaagttcgc cgtgcgctgt
    10561 atcttggcaa aatcgtttct tacgctcagg gcttctctca gctgcgtgca gcgtctgaag
    10621 agtacaactg ggatctgaac tacggcgaaa tcgcgaagat tttccgtgct ggttgcatca
    10681 ttcgtgcgca gttcttgcag aaaatcaccg atgcttatgc cgaaaatccg cagatcgcta
    10741 acctgctgct ggctccgtac ttcaagcaaa ttgccgatga ctaccagcag gcgctgcgtg
    10801 atgtcgttgc ttatgcagta cagaacggta tcccggttcc gaccttcgct gctgcggttg
    10861 cctattacga tagctaccgt gccgctgttc tgcctgcgaa cctgatccag gcacagcgtg
    10921 actatttcgg tgcgcatact tataagcgca ttgataaaga aggtgtgttc catactgaat
    10981 ggctggatta a
//

</pre>