<pre>LOCUS       DQ990684               13283 bp    DNA     linear   BCT 27-MAY-2010
DEFINITION  Escherichia coli strain O118 O-antigen gene cluster, complete
            sequence.
ACCESSION   DQ990684
VERSION     DQ990684.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13283)
  AUTHORS   Liu,Y., Fratamico,P., Debroy,C., Bumbaugh,A.C. and Allen,J.W.
  TITLE     DNA sequencing and identification of serogroup-specific genes in
            the Escherichia coli O118 O antigen gene cluster and demonstration
            of antigenic diversity but only minor variation in DNA sequence of
            the O antigen clusters of E. coli O118 and O151
  JOURNAL   Foodborne Pathog. Dis. 5 (4), 449-457 (2008)
   PUBMED   18673069
REFERENCE   2  (bases 1 to 13283)
  AUTHORS   Liu,Y., Fratamico,P. and DebRoy,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2006) Microbial Food Safety Research Unit,
            ERRC-ARS-USDA, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA
FEATURES             Location/Qualifiers
     source          1..13283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="O118"
                     /db_xref="taxon:562"
     misc_feature    143..12776
                     /note="O-antigen gene cluster"
     gene            143..1501
                     /gene="wbhU"
     CDS             143..1501
                     /gene="wbhU"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphorylase"
                     /protein_id="ABM53639.1"
                     /translation="MKNIAVILASGTGSRFGAKIPKQFVKLAGKPVIQYTLHAFQNAK
                     KIDEIIVVTNEEYVDFVSALTMEHNYFKVTKIIHGGKERYESSWAALQSIELDECNVI
                     FHDAVRPFVSQKIIDDCIDGLNNYNAIDVVVDPTDTIVKIKNKEIMSIPDRRFLARGQ
                     TPQGFKKSLIEKAYIDFLKEEKKVASDDCGIFLKYMPNEPIGIIKGDESNFKITHQQD
                     IYLADNLIKDGLLELISENNDSIARTISEKVIVIFGGNSGIGKTLLVKAKSLDGNVYS
                     FSRSNGIDISNAMNVRKALQSVFEKEGRIDYIINTAAVLIKKPINFMSDEDIEQSCNT
                     NYLGAINIAREGYGYLKKTKGMLINFTSSSFTRGRASYSLYSSSKAAVVNLTQALSEE
                     WLNLGIKVNCINPERTDTPMRRVNFGIEPPESLLTSEQVADMTLSIMSSDYTGQVVAV
                     KK"
     gene            1506..2726
                     /gene="wzx"
     CDS             1506..2726
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="ABM53640.1"
                     /translation="MSLRKNISGLAIAQIFSYIAPLLQFPYLSRIVGDSYLGAIIFIL
                     SISQMAMVLTDYGFDLSIGEQIARGKRNNKWLSVFYCQTGVIKSFLLVVAFVFIISVI
                     RLSKSDIPEQLIIGLMICVTLNAYNPYWIFQGLEKIYIYSALVIISRLIGLVLIYLLI
                     RTPDDYVYYSWILAFQAGMTTIISHIIISRWGVRVCRIKVKGIIKQFKYSFDFFLSRA
                     CVSLYSSGCSVFLATFGGSLNQVAIYGVAEQLYKAGVQVFSPVISALTPYMVRTKNYI
                     IFYKIIVVCITVTVIGMCVGFLFGAQIITLIYGNGYQESKSVLDVFMVVILASVFGML
                     LGYPALLPLGLGKVANYSVIASGILQIIMFCILYIGLFPITAISVCLTYLICDWFMFF
                     IRLYNFMKYRKING"
     gene            2719..3537
                     /gene="wbhV"
     CDS             2719..3537
                     /gene="wbhV"
                     /codon_start=1
                     /transl_table=11
                     /product="LicD-family phosphotransferase"
                     /protein_id="ABM53641.1"
                     /translation="MDNQLRAAQMRMLAILNEVDRICRLNKINYWLDAGTLLGAKRHG
                     GFIPWDDDIDICMLRDDYNKFIMACDKQLNKEIFFLQTAYSDEYYVDFNTPCKIRMNN
                     TEIIEKSEVKYKYYDERSHHGLFLDIFPYDKYSSNKYIRKYVERFISQLCKLKVLSKY
                     SGFPITKKILIKCLSSIICKRRLLAFVDLVSFRMSERKHDYLLGAGIETPFHRAYFSE
                     DTIFPLSEIKFEGRMYLCPNNVDKYLCMMFGDNYMTIPPAAQRVWHYHSINIGK"
     gene            3544..4644
                     /gene="wzy"
     CDS             3544..4644
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="ABM53642.1"
                     /translation="MVEGFVIYNSIVLLALLFCYLKRFFLPRYFTVCLFLVLWVPLAL
                     RYGIGRDYFSYIEIYKNVLVARDGIELGFYYINYILAYFNFHYQYLFALSSFITVYFC
                     VKSFDRDYTLVSIILFCALIYLQAFSIIRQMIAVSLCLYSCSLWNKGCKCKSSVFLIL
                     APLFHFSAIIILLLAIASRYIKINTTRCYFVFVFALVFIFILNGVDFIFSNAILLDSK
                     YGYYVTSAFNRPTEIGSGIGVAIALFLPFMVFIKSTQLYEYNKNYNFMLLINLVYVIS
                     YTLSLKIYIFARFTDVLSFVQILLIPVILKISSPKGIYYNLMMAVILLHILLFEANLN
                     ANTISAGNISNSGLGIMPYKSILNTKIVGIYD"
     gene            4637..5746
                     /gene="wbhW"
     CDS             4637..5746
                     /gene="wbhW"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="ABM53643.1"
                     /translation="MTSKILFITPLPPPVTGQSLACIELYNYLDKDNNCIIDVINLSK
                     QSFISGKGSFKRVAEIAGVLLRLLFLRRDYDLIYFTPAESKMGSYKDQLIYLFMFAKL
                     KKMYIHLHGGAGMKKLLEKDKSLIFKLNKYFISRLAGVIVLGERLKSIYNGIIESDKI
                     YAVENFSQDYLFIRQSELKLKFNNRNKIKILFLSNLLPGKGYIELLKSADFLDDSCVE
                     FHFAGGFEDEQAKKIFLEAINLKNNIFYHGIVSGDEKRNLLIKSDIFCLPTYYPYEGQ
                     PISILEAYAAGCVVLTTNHSGIFDVFEPWRNGIEVDKKSTSDLVNAINYFNSNREEIR
                     KIAINNIVDARKKYRLKHHLEKMMTVLMNHEVIQQ"
     gene            5743..6879
                     /gene="wbhX"
     CDS             5743..6879
                     /gene="wbhX"
                     /codon_start=1
                     /transl_table=11
                     /product="amidotransferase"
                     /protein_id="ABM53644.1"
                     /translation="MKKYKICNRCVMDTSDPNISFDDNGQCDYCSNFDKNILPNWNAN
                     NSGLSKLLEMSESIKKDSKGSDFDCIIGLSGGLDSSYAAYIAKEKMGLRPLLFHVDAG
                     WNTDQAVGNIEKLIEGLGLDLYTEVINWEEMKDLQLSFLKSGIPDQDLVQDAAFFSSL
                     YKFARKHKIKHVITGSNFSTECCREPEEWGGYLGIDTMLFNDIHRQFGTRPLNSFPLV
                     DILIYKVFYQKILGMKVHHPLNLVPFTKKDAENELSEKFGWQPFQHKHHESRFTRFYE
                     DYWLPRRFGYEKRRAHFSSLIMTGQMTREQALERLLKPEMDEHFLEQEFEYIAHKLGI
                     SVDDLKELFSIPKKTYKDYKNKRWLISLGTNILRTLGLEKRYFR"
     gene            6876..7502
                     /gene="wbhY"
     CDS             6876..7502
                     /gene="wbhY"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="ABM53645.1"
                     /translation="MIKIIDYGIGNIQAFLNVYKRLGINSGIAHSIDDIKSATHLILP
                     GVGAFDQAMTLFNNSGLRESIEKRIFEDRVPIIGICVGMQMLATSSEEGSLPGLGWIP
                     GTVRAFSSNISSRSLPMPHMGWNSLIKKVNTPLLKDFKEESSFYFLHSFYYECDDAGD
                     VLATAEYGHNFHCVIGRNNIYGIQCHPEKSHSSGYQLLKNFAEIQYNA"
     gene            7495..8283
                     /gene="wbhZ"
     CDS             7495..8283
                     /gene="wbhZ"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol phosphate synthase"
                     /protein_id="ABM53646.1"
                     /translation="MLRPRIIPCLLVHDSGLVKTVNFKSPKYVGDPINAVKIFNEKEA
                     DELMVLDIDATSRGLEPNYALIKKLAAECRMPLCYGGGVTSVAQAVKIISLGVEKVSI
                     SSAAVENPNIVKDLAEAVGKQSVVVVLDVIKRKGLFSKGYELSTRNNTRTYKIDPISF
                     AQKMVALGAGEIVINFVDNDGVMGGYDVEYSSTIKSQINVPVTFLGGAGNYEHLSALI
                     DQCGIVGAAAGSLFVFKGKYRAVLISYPTPEQKDIICNNLKKNY"
     gene            8293..9330
                     /gene="fnlA"
     CDS             8293..9330
                     /gene="fnlA"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucosamine synthetase"
                     /protein_id="ABM53647.1"
                     /translation="MMFKDKILLITGGTGSFGNAVLNRFLSTDIKEIRIFSRDEKKQD
                     DMRKKYNNQKLKFYIGDVRDYRSILNATRGVDFIYHAAALKQVPSCEFHPMEAVKTNI
                     LGTENVLEAAIANEVKRVVCLSTDKAVYPINAMGISKAMMEKVMVAKSRNVDRNKTVI
                     CGTRYGNVMASRGSVIPLFVDLIRAGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGN
                     NGDIFVQKAPAATIDTLAIALKELLNVPDHPVNVIGTRHGEKLYEALLSREEMIAAID
                     MGDYYRVPPDLRDLNYGKYVEQGDSRISEIEDYNSHNTQRLDVEGMKELLLKLAFIRA
                     IRAGEKYNLDS"
     gene            9332..10435
                     /gene="fnlB"
     CDS             9332..10435
                     /gene="fnlB"
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="ABM53648.1"
                     /translation="MRILVTGANGFIGRNLCLRLEELGYKDLIRIDRESTKQDLEQGL
                     QDADFIYHLAGINRPKTDDEFISGNSDLTKHIVEYLLSIGKNTPIMLSSSIQAELNNA
                     YGVSKAVAESYVEKYAAASGSSYYIFRYPNVFGKWCKPNYNSFIATFCYNISNDIEIT
                     INDASAPVNLVYIDDVCTDAIALLSGTVESGYKVVAPIYSTTVGEVAELIYSFKNSRS
                     TLITEAVGTGFTRALYSTWLSYLPAEKFAYKVPFYGDARGVFCEMLKTPSAGQFSFFT
                     AHPGITRGGHYHHSKNEKFLVIRGQACFKFEHVITGERYELKVSSGEFKIVETVPGWT
                     HDITNIGTEELIVMLWANEIFNRDEPDTIARPL"
     gene            10435..11565
                     /gene="fnlC"
     CDS             10435..11565
                     /gene="fnlC"
                     /codon_start=1
                     /transl_table=11
                     /product="C-2 epimerase"
                     /protein_id="ABM53649.1"
                     /translation="MKKLKVMSVVGTRPEIIRLSRVLAKFDEYCEHIIVHTGQNYDYE
                     LNEVFFNDLGVRKPDYFLNAAGKNAAETIGQVIIKVDEVLEIEKPEAILVLGDTNSCI
                     SAIPAKRRKVPIFHMEAGNRCFDQRVPEETNRRIVDHTADINMTYSDIAREYLLAEGI
                     PADRIIKTGSPMYEVLSYYMPQIDGSDVLSRLNLQSGEFFVVSAHREENVDSPKQLVK
                     LANILNTVAEKYNLPVIVSTHPRTRNRIREQGIEFHSNINLLKPLGFHDYNHLQKNSR
                     AVLSDSGTITEESSIMNFPAVNIREAHERPEGFEEASVMMVGLECERVLQALDILATQ
                     PRGEVRLLRQVSDYSMPNVSDKVVRIVHSYTDYVKRVVWKEY"
     gene            11565..12776
                     /gene="wbfG"
     CDS             11565..12776
                     /gene="wbfG"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucosamine transferase"
                     /protein_id="ABM53650.1"
                     /translation="MKLALIIDDYLPNSTRVGAKMFHELAQEFIQRGHDVTVITPGTG
                     MQEEISFDTFQGVKTWRFKSGPLKDVSKIQRAVNETLLSYRAWKAIKKWVKKETFEGV
                     IYYSPSIFWGHLVKKIKARCQCPAYLILRDMFPQWVIDAGMLNAGSPIERYFRLFEKI
                     SYRQANRIGLMSDKNLDVFRKDNKGYPCEVLRNWASLTPTIIPKDYIPLRKRLGLEDK
                     TIFFYGGNIGHAQDMTNLMRLVRNMAVYPQAHFLFIGQGDEVELINSLASEWALTNFT
                     YLPSVNQDEFKFILSEMDIGLFSLSARHSSHNFPGKLLGYMVQSLPILGSVNAGNDLL
                     DIVNQNNAGLIHVNGEDDKLCQSALLMLHDIDVRRQLGLGANILLKEQFSVESAAQTI
                     EMRLEACNAIN"
ORIGIN      
        1 attttttgat tatgtgcgat taaattaaaa tcgtatatca tttagagtta tatctgttct
       61 ggcactcaac tccatttttg gggtaagatt atataggtta tttcgatatc ttgataacag
      121 acttaataga ttaaggggtc ttatgaaaaa tattgcagta attcttgcat caggcactgg
      181 aagtcggttt ggggctaaaa tacctaaaca attcgttaaa cttgcaggaa aacctgtaat
      241 tcaatatact ctacatgcct tccaaaatgc aaagaaaatt gatgaaatta tagttgttac
      301 aaatgaagaa tatgtggact ttgtgtctgc tttgactatg gaacataact actttaaggt
      361 tacgaaaata atccatggtg gtaaggaacg ctatgaatct tcgtgggcag cgttacaaag
      421 tatagaatta gatgaatgta atgtgatatt tcatgacgcc gtgcgaccgt ttgtttctca
      481 aaaaatcatc gatgactgta ttgatggtct taataattac aatgcaattg acgtggtcgt
      541 agatccaacc gatacgattg taaaaatcaa aaataaagag attatgtcta ttcctgacag
      601 aaggttttta gctagagggc aaactccaca aggttttaaa aaatcattaa ttgaaaaagc
      661 atatattgat tttttaaagg aagaaaaaaa agttgcttct gatgattgtg gtattttcct
      721 caaatacatg ccaaacgaac ccatagggat tattaaaggc gatgaatcta attttaaaat
      781 aacgcaccag caagacattt atcttgcgga taatttaatt aaggatggtt tgctggagtt
      841 aatatctgag aataatgatt caattgcgcg aacgatatca gaaaaagtca tagttatatt
      901 tggagggaat agtggtatag ggaaaactct tttagtaaaa gctaaatctc ttgacggaaa
      961 tgtttattca ttcagtcgta gcaatggaat tgacatcagt aatgccatga acgtaagaaa
     1021 ggcattacag tctgtattcg aaaaagaagg tcgaattgat tatataataa atacagctgc
     1081 agttttgata aaaaaaccaa ttaattttat gtcagatgag gatatagaac aaagctgtaa
     1141 tacaaactat ttaggggcaa ttaatatagc tagagaaggc tatggttatt taaagaagac
     1201 aaaagggatg ttaattaact ttacttctag ctcatttaca cgtggaagag cttcatatag
     1261 tctttattca tcatctaaag cagctgtggt gaatttaaca caggccttat cagaagagtg
     1321 gttaaatttg ggaattaaag ttaactgtat aaatccagag cgtacagata ctcctatgag
     1381 aagagtcaat tttggtattg aaccaccaga atccttacta acctcagaac aggttgcgga
     1441 catgacttta tcaattatgt cctctgatta tacagggcaa gtagtagctg tgaaaaaata
     1501 acttcatgtc attgcgaaaa aatattagcg gcttagctat agctcaaata tttagctata
     1561 tagcaccatt actgcaattc ccttatctaa gtaggattgt aggggattca tatttaggtg
     1621 ctattatttt tattctctca atatcacaaa tggcaatggt gttgacagat tatgggtttg
     1681 atttatcgat tggagaacag atagcaagag ggaaaagaaa taataaatgg ttaagcgttt
     1741 tttattgtca aactggagta attaaatcat ttctattagt ggtagcattc gtatttatta
     1801 taagcgtgat tcgcctgtca aaaagtgata ttcctgagca actgataatt ggattaatga
     1861 tttgtgttac attaaatgct tataatccat attggatatt tcagggcctt gaaaagatat
     1921 atatatatag tgcattagta atcatttcaa gattaatagg gcttgtttta atctatttgc
     1981 taattaggac tccagatgat tatgtatatt actcttggat tttggcattt caagcgggga
     2041 tgacaacgat aatcagccat attattatat ccagatgggg ggttcgtgtt tgtcggataa
     2101 aagtaaaagg tataattaag caatttaaat atagttttga ctttttttta tcaagagctt
     2161 gtgtttcatt atactcatct ggttgcagtg ttttcttagc aacatttggt gggagtctga
     2221 atcaagttgc gatctatggc gttgcagaac agttatataa agcgggagta caggtattta
     2281 gccccgttat ttcagcgcta actccttaca tggtaagaac aaaaaactat attatttttt
     2341 acaaaattat agtggtatgc ataactgtca cagtgatagg tatgtgtgtc gggtttttat
     2401 ttggtgctca aatcattaca ttgatttatg gtaatggata tcaggaatcc aaatctgtac
     2461 tagatgtctt tatggttgta attcttgcaa gtgtatttgg tatgctttta ggatatcctg
     2521 cattgcttcc cttaggattg ggtaaggttg ctaattatag tgtcattgct tctggtatat
     2581 tgcaaataat aatgttctgt attttgtata ttggtttatt tcctattaca gctattagtg
     2641 tatgtctgac atatctaatt tgtgattggt ttatgttctt tattcgtctt tataacttta
     2701 tgaaatatag gaaaattaat ggataatcag ttacgtgcag cccaaatgag gatgctggca
     2761 attcttaacg aagttgatcg aatatgcaga ctgaataaaa taaattattg gctagatgca
     2821 gggaccttgt taggtgccaa aagacatggt ggttttattc cttgggatga tgatatagat
     2881 atttgtatgt taagggatga ttataataaa tttattatgg catgtgacaa acaacttaat
     2941 aaagagatct tttttcttca aacagcttat tcagatgaat attatgtgga ctttaataca
     3001 ccatgtaaaa taagaatgaa taatacggag attatagaaa agagtgaagt gaaatataaa
     3061 tactacgatg aaagatctca tcatggtttg tttcttgata tattccccta tgataaatat
     3121 tcatctaata aatatattag gaagtatgtt gaacggttta tatctcaact atgtaaactt
     3181 aaagtattat caaaatactc tggatttcct attacaaaaa aaatacttat aaaatgttta
     3241 agtagtatca tttgtaaaag acgattatta gcttttgtag atttagttag ttttcggatg
     3301 agtgagagga aacatgatta tttactaggc gctggaattg aaacaccatt tcatagagcc
     3361 tacttttctg aggatacaat ctttccatta agtgaaataa aatttgaagg aaggatgtac
     3421 ttatgcccaa ataatgttga taagtatctc tgtatgatgt ttggggacaa ttatatgact
     3481 attccacctg cagctcagag ggtgtggcac taccatagca ttaatattgg gaaatagact
     3541 actatggttg aagggttcgt tatttacaat agcatagtct tgcttgcttt attattttgt
     3601 tatttgaaga gatttttttt accccgttac tttactgtct gcttattttt ggttctttgg
     3661 gttccattag cgcttaggta tggtataggg cgtgattatt ttagctatat tgaaatttat
     3721 aaaaatgttc tagttgcaag agatggtatt gagctgggtt tttattatat aaattatata
     3781 cttgcttatt ttaattttca ctatcaatat ttatttgcac tgtcatcctt cattacagtt
     3841 tatttttgtg taaaaagctt tgatagagat tatacgttag tatctattat attattttgt
     3901 gctttaattt acttgcaggc attcagtatt attagacaaa tgattgcagt gtcactctgt
     3961 ctctatagtt gctcattatg gaataaaggt tgtaaatgta aatcatcagt attccttata
     4021 ttggcacctt tatttcattt ctctgcaata ataatattac ttttagctat agcaagccga
     4081 tatataaaaa taaatacgac aagatgttat tttgtatttg tttttgctct tgtattcata
     4141 tttatattga atggggtaga ttttatattt tcaaacgcta tattattaga tagtaagtat
     4201 ggatactatg taacatcagc atttaatcga cctacagaaa ttggctcagg aataggtgtt
     4261 gctatagcat tatttctacc gttcatggta tttataaaat caactcagtt atatgagtat
     4321 aataaaaact ataattttat gctgcttata aatctagtat atgttatttc ttatacactt
     4381 tctctgaaaa tatatatatt tgcacgattt acagatgttc tatcttttgt tcagatatta
     4441 ttgataccag taatacttaa aatctcttcc cctaaaggga tttattataa tttaatgatg
     4501 gctgttattt tgcttcatat tttattattt gaagcaaacc ttaatgcgaa tacaatttct
     4561 gcaggaaata tttcgaatag cggattaggc atcatgccat ataaaagcat actaaatact
     4621 aaaatagtag gtatatatga ctagtaaaat attgtttata actccattgc ctccaccagt
     4681 tactggacaa tctttagctt gtatcgagtt atataattat ctagataaag ataataattg
     4741 tattattgat gtaataaatc tttcaaaaca gtcctttata tcagggaaag gctcgttcaa
     4801 aagagttgct gaaatagctg gtgtgttatt acgtttatta tttcttagac gagattatga
     4861 tttaatttac tttacaccag ctgaatctaa aatgggaagt tataaagatc aattgatata
     4921 tctttttatg tttgcaaagc taaagaaaat gtatattcac ttgcatggcg gtgcaggcat
     4981 gaaaaaatta ttagaaaaag ataaatcatt aatcttcaaa ttaaataaat atttcatctc
     5041 taggcttgct ggagtgatag tgctgggtga aagattaaag agtatttata atggaattat
     5101 tgaaagtgat aaaatttatg cagttgagaa cttttctcaa gattatttat ttataaggca
     5161 aagtgagtta aaattaaaat ttaataatcg gaacaaaata aaaatacttt ttcttagtaa
     5221 tttgttgcca ggaaaaggat atattgaatt gttaaaatct gcagattttt tagatgattc
     5281 ttgtgtggaa ttccatttcg ctggtggttt cgaggatgaa caagcaaaaa aaattttttt
     5341 agaggctatt aatttaaaga acaatatttt ctatcatggg attgtcagtg gcgatgaaaa
     5401 acggaatcta ctaattaaat ctgatatttt ttgtttacca acatattatc cttatgaggg
     5461 gcaacctata agcatacttg aggcatatgc tgctgggtgt gttgttctta ctaccaacca
     5521 cagtggtata tttgatgtgt ttgaaccttg gcgtaatgga atagaagttg ataaaaaaag
     5581 cacatctgat ttagtaaatg caataaatta ttttaattcg aacagagaag agattcgtaa
     5641 aattgccatc aataatattg ttgatgcaag aaaaaaatat agacttaaac atcatttaga
     5701 aaagatgatg acagtattaa tgaatcatga ggtaatacag caatgaaaaa atataagata
     5761 tgcaatagat gtgttatgga tacatctgat ccaaacattt catttgacga taatggccag
     5821 tgtgattatt gttcaaactt tgataaaaat attttaccaa attggaatgc taataattca
     5881 ggcttatcaa aattgcttga aatgtcagaa tcaattaaaa aagacagtaa gggaagtgat
     5941 tttgattgta ttattggctt atcaggaggt ttagatagct cttatgcagc gtatattgcg
     6001 aaagaaaaaa tgggactacg cccattatta tttcatgttg atgctggatg gaatactgat
     6061 caggcagttg gtaatattga aaagcttatc gaaggacttg gtttagatct ttacacagaa
     6121 gttataaact gggaagaaat gaaagatctt caactgtctt tcttaaaatc aggaattcca
     6181 gatcaagatt tagttcagga tgctgcattt ttttcatctt tatataagtt tgctcggaaa
     6241 cataaaatta aacatgttat tactggttca aatttttcta cagagtgctg tcgcgaacca
     6301 gaagaatggg gagggtattt aggtattgat actatgttat ttaatgatat ccacagacag
     6361 tttggaaccc ggccattaaa ttcattcccc ttagtagata tactcatcta taaggttttt
     6421 tatcagaaaa tccttggaat gaaagtacat catccattga atttagtacc atttactaag
     6481 aaggatgccg aaaatgaact tagtgaaaaa tttggctggc aaccttttca acataagcac
     6541 catgagtccc gttttactcg tttctatgaa gattattggt taccacgtag gtttggttac
     6601 gaaaaacgta gagctcattt ctcaagtcta attatgacag ggcaaatgac tcgcgaacaa
     6661 gctcttgaga ggcttttaaa gccagaaatg gatgaacatt ttcttgaaca agagtttgag
     6721 tatattgcac acaaactagg cattagtgtt gacgatttaa aagaactatt tagtattccc
     6781 aaaaaaacct acaaggatta taaaaataag cgttggttga ttagtttagg tacaaatatc
     6841 ctacgaacac ttggattgga aaaaaggtat ttcagatgat caaaataatt gattatggta
     6901 ttggaaatat acaagcattc cttaatgtat ataagcgatt aggtattaat tcaggtattg
     6961 ctcattctat cgatgatatt aaaagtgcta cacatttgat attacctgga gttggagctt
     7021 ttgaccaagc tatgacactt tttaataact caggtttacg agaaagtatt gaaaaacgta
     7081 tttttgagga tcgggttcca attattggta tctgtgtagg tatgcagatg cttgctacct
     7141 ctagtgagga ggggagtttg cctggtttag gatggattcc tggtacggtt cgagcattca
     7201 gcagtaatat ctcttcaaga agcctcccga tgcctcatat gggatggaat agtttaatta
     7261 aaaaagtaaa tactccatta ctgaaagatt ttaaagaaga gtcttccttc tattttttgc
     7321 actcattcta ttacgaatgt gatgacgccg gtgatgtatt agcaacagct gaatatggtc
     7381 ataattttca ttgtgtcatt ggcaggaata atatatatgg cattcaatgt catccggaga
     7441 aaagtcattc atcaggttat caactcttaa aaaattttgc agagatccaa tataatgctt
     7501 agaccaagaa tcataccctg tttacttgtc cacgattctg gcttagtcaa aacagttaat
     7561 ttcaaatcac cgaagtatgt tggtgatcca attaatgccg ttaaaatttt taatgagaaa
     7621 gaagctgatg aattgatggt ccttgatatt gatgcaactt caaggggact tgaacctaat
     7681 tatgctctaa taaaaaaact cgcagcggaa tgccgaatgc cattatgcta tggaggaggt
     7741 gtaactagtg tagcacaggc tgtaaaaatt atctctcttg gtgttgaaaa ggtatcgata
     7801 agttcagcgg ccgttgaaaa tccaaatatt gtaaaagatt tggcagaagc cgttggtaaa
     7861 caaagtgttg tagttgtgct agatgttatt aaacgtaaag gtcttttttc aaagggttat
     7921 gagctttcga ctaggaataa cacgcgaacg tataaaatcg atcctatctc gtttgctcaa
     7981 aaaatggttg ctttgggtgc aggcgaaatc gtcatcaatt ttgtagataa tgatggtgtt
     8041 atggggggat atgatgttga atattcttcg actatcaaat cgcaaataaa cgtacctgtt
     8101 acatttctcg gtggtgcggg aaactatgaa catctctctg cattgattga ccaatgtggc
     8161 atcgttggtg cagcagctgg tagtcttttt gtatttaaag gtaagtatag agctgtgttg
     8221 ataagctacc ctacacctga gcagaaagat attatttgca ataatttaaa gaagaattat
     8281 taataagggg ttatgatgtt caaagataaa atactgttaa ttactggtgg tacaggttca
     8341 tttggtaatg ctgtattaaa ccgttttctt tctacagata taaaagaaat tcgtattttt
     8401 tctcgcgatg aaaagaaaca agatgatatg cggaaaaaat ataataatca gaagctgaaa
     8461 ttttatattg gtgatgtcag agattaccgt agtattttga atgcgactcg cggtgttgat
     8521 tttatatatc atgcagcggc acttaagcaa gttccatcat gtgaatttca tcctatggaa
     8581 gccgttaaaa ctaatatcct tggtacggaa aatgttcttg aagcagctat agcgaatgaa
     8641 gtgaagaggg ttgtatgcct aagtactgat aaagctgtat acccgattaa cgcaatgggt
     8701 atttcaaaag ctatgatgga aaaggtcatg gtcgcgaaat cccgtaatgt tgatcgcaat
     8761 aaaacagtaa tatgtggtac ccgttatggg aatgttatgg catctcgcgg ttcagttatt
     8821 ccattatttg ttgatcttat tagagcgggc aagccactca caataactga tcctaatatg
     8881 acccgcttta tgatgactct tgaggatgcg gtagatttag ttctttatgc gtttgaacat
     8941 ggtaataatg gtgatatctt tgtgcaaaaa gcacctgcag caactattga cacattagct
     9001 attgctttaa aggaactact aaatgttcca gaccatccgg taaatgtcat tggaacgcgt
     9061 catggcgaga aattatatga agctctactt agtcgtgagg aaatgatcgc tgctatagat
     9121 atgggcgatt attaccgtgt cccgccagat cttcgggacc ttaattatgg aaaatatgtt
     9181 gagcaaggtg atagccgaat atctgaaata gaagattata actctcataa tactcaacgg
     9241 ttagatgttg aaggcatgaa agagctcttg ctaaaattag cctttattcg agcaattcgt
     9301 gctggtgaaa aatataatct ggattcatga tatgagaata ttagttactg gtgcaaatgg
     9361 ttttattggt cgtaatttat gtttgaggct tgaggaactt ggttataaag atcttattag
     9421 aattgatcga gaatcaacga agcaagatct tgaacaaggc ttacaggatg ccgattttat
     9481 ttatcactta gctggtatca atagacctaa gactgatgat gagtttattt ctggaaacag
     9541 tgatttaaca aagcatatag ttgagtatct cctttctatt ggtaagaata caccaattat
     9601 gctaagttct tcgatacaag ctgaacttaa taatgcttat ggggttagca aagctgtagc
     9661 tgaaagctat gtcgaaaaat atgctgctgc tagtggttct tcgtattata ttttcagata
     9721 tccaaacgtt tttggtaaat ggtgtaagcc aaactataat tcttttatag caactttttg
     9781 ctacaatatt tccaatgata ttgagattac tatcaatgat gcatcagcgc cagttaatct
     9841 ggtgtatatt gatgatgttt gtactgatgc tatagctctt ctctctggga cggttgaaag
     9901 cggatataaa gttgttgcac caatttattc aacaacagtt ggtgaggttg cagaattaat
     9961 ttatagcttc aaaaatagcc gttccaccct gatcacagaa gctgtcggga cgggatttac
    10021 ccgtgcattg tattctacat ggctgagtta tttaccagca gagaagtttg cgtacaaggt
    10081 acctttttat ggggatgccc gcggagtctt ttgtgagatg ttgaaaacgc cttcagcggg
    10141 gcagttttca ttttttactg ctcaccctgg tattacgcgt ggcggacatt accatcacag
    10201 taaaaatgag aagtttttgg tcattcgagg tcaggcatgc tttaaatttg aacatgtgat
    10261 taccggtgag cgatatgaac tgaaagtttc atcgggtgag tttaagatcg ttgaaacagt
    10321 tcctggttgg acacatgaca ttacaaatat tggaactgag gaattaatag tcatgctctg
    10381 ggcaaatgaa attttcaacc gtgatgagcc cgatactatt gcgaggcctc tataatgaaa
    10441 aaattaaaag ttatgtctgt tgttggaacc cgtcctgaga ttatccgttt gtcgagggtt
    10501 cttgctaagt ttgatgaata ctgcgagcat attattgtcc atactggtca aaattatgat
    10561 tacgaattaa atgaagtgtt cttcaatgac ttgggtgttc gaaaacctga ttatttttta
    10621 aatgcagcgg gtaaaaatgc ggcggaaacc attggccagg ttattattaa ggtagatgaa
    10681 gtattagaaa tcgaaaaacc tgaagcaata ctggtattgg gcgatacgaa ttcatgtatt
    10741 tctgccattc cggccaaacg ccgtaaagtg cctatatttc atatggaagc aggtaaccgt
    10801 tgtttcgatc aacgcgtgcc tgaagaaacc aatagacgta ttgttgacca tacggctgat
    10861 atcaatatga cctacagtga tattgctcgt gaatatctct tggctgaagg tatcccagct
    10921 gatcggatca taaaaactgg tagccctatg tatgaggttc tttcatatta tatgccccaa
    10981 attgatggtt cagatgtgct atcgcgtttg aatctacagt ctggtgagtt ttttgtagta
    11041 agtgcgcatc gtgaagagaa tgttgattct ccaaaacagc tcgtaaagct tgcgaacatt
    11101 ctaaatactg ttgctgaaaa atataatctt ccagttattg tctccacaca cccaaggaca
    11161 cgtaaccgaa tccgtgagca aggaattgaa tttcattcaa atataaattt actgaaacca
    11221 ttgggtttcc atgattataa ccacttgcag aagaactcac gagctgtgct ttcagatagc
    11281 ggtactatca ctgaagagtc atccatcatg aatttcccag cggtaaacat ccgggaagcg
    11341 catgagcgtc cggaaggctt tgaggaagca tccgtcatga tggtggggtt agagtgtgaa
    11401 cgcgtattac aagcgctgga tattctggca acacaaccgc gaggtgaagt ccgtctttta
    11461 cgtcaggtta gtgattacag catgccaaat gtgtcggata aagttgtcag aattgttcac
    11521 tcttacacag attatgttaa gagagtcgtc tggaaagaat attgatgaaa cttgctttaa
    11581 tcatagatga ttacctgccc aacagtactc gtgttggtgc aaaaatgttt catgaacttg
    11641 ctcaagaatt tatccagcgt gggcacgatg ttacggtaat tactcctggt acgggcatgc
    11701 aagaagagat ttcttttgat acctttcagg gggtaaaaac atggcgtttt aaaagcgggc
    11761 cgctcaagga tgtaagtaaa attcagcgag cggtcaatga aacgcttttg tcctatcggg
    11821 cgtggaaagc catcaaaaaa tgggtaaaaa aagagacctt tgagggtgtg atttattatt
    11881 caccttccat attctggggg catttagtta aaaaaattaa agctcgttgc caatgtcctg
    11941 cttatcttat tttaagagat atgtttccac aatgggtaat tgatgcagga atgcttaatg
    12001 ctggttcccc aatagaacgc tactttcgtc tttttgaaaa aatatcttat cgtcaggcaa
    12061 atcgtattgg acttatgtct gataagaatc ttgatgtttt tcggaaagat aataaaggct
    12121 atccgtgcga agttttgcgt aactgggcat ccctaacacc aacgatcata cccaaggatt
    12181 atataccact acgtaagcga cttggcctag aggataaaac cattttcttc tatggtggaa
    12241 acataggtca tgcacaggac atgacaaact tgatgcgact tgtgagaaac atggcagtat
    12301 atcctcaagc tcatttccta tttattggcc agggtgatga agttgaatta attaattcat
    12361 tagcctctga gtgggcattg acgaatttca cctatttgcc ctcggttaat caggatgaat
    12421 ttaagttcat tttgtcggaa atggatatcg gcttgttttc tctttccgct agacactctt
    12481 cccataattt tcctggtaag ttattaggct atatggttca gtcgctacct attttaggta
    12541 gcgtaaatgc cggaaatgat ttgctcgaca ttgtcaatca aaataatgcc ggattaatcc
    12601 atgtcaatgg tgaggacgat aaattatgtc aatctgcgct attaatgttg catgatattg
    12661 atgtgcgccg gcaacttggt ttgggagcga atatattgtt gaaagaacaa ttctccgttg
    12721 agtctgcggc acagacgata gaaatgaggt tggaggcatg caatgcgatt aattgataat
    12781 gaccaactcg acaaattata tgatcaagcc gggcaatcgg aacgtttacg ttcccacctt
    12841 atgatgcacg gctcgcatca agaaaaggta cagcgtttac ttattgcatt agtaaagggc
    12901 agctatgttg aaccgcatta tcacgcactt cctcatcagt gggaaatgtt catagttatg
    12961 gaggggcaac ttcaggtttg tttgtatggt agaaatggtg aggttataaa gcaatttata
    13021 gcaggagata atactgggat aagcattgtg gagttttctc cgggcgatat acacagtgtc
    13081 gaatgcctat ctcctcgtgc tcttatggtg gaagttaaag aggggccgtt tgactcttct
    13141 tttgcaaaat tgttcgtgtg atgctcgtct aaaatgcatc ttctgctatc tacccaagct
    13201 aaacctgagt taacatccat accatatttc aagccgcgaa tatcttgcgc ggtgaccacc
    13261 cctgacagga gtatacaatg tca
//

</pre>