LOCUS       AF172324               14263 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Escherichia coli GalF (galF) gene, partial cds; O-antigen repeat
            unit transporter Wzx (wzx), WbnA (wbnA), O-antigen polymerase Wzy
            (wzy), WbnB (wbnB), WbnC (wbnC), WbnD (wbnD), WbnE (wbnE),
            UDP-Glc-4-epimerase GalE (galE), 6-phosphogluconate dehydrogenase
            Gnd (gnd), UDP-Glc-6-dehydrogenase Ugd (ugd), and WbnF (wbnF)
            genes, complete cds; and chain length determinant Wzz (wzz) gene,
            partial cds.
ACCESSION   AF172324
VERSION     AF172324.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14263)
  AUTHORS   Paton,A.W. and Paton,J.C.
  TITLE     Molecular characterization of the locus encoding biosynthesis of
            the lipopolysaccharide O antigen of Escherichia coli serotype O113
  JOURNAL   Infect. Immun. 67 (11), 5930-5937 (1999)
   PUBMED   10531250
REFERENCE   2  (bases 1 to 14263)
  AUTHORS   Paton,A.W. and Paton,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUL-1999) Molecular Microbiology Unit, Women's and
            Children's Hospital, King William Road, North Adelaide, S.A.,
            Australia
FEATURES             Location/Qualifiers
     source          1..14263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="98NK2"
                     /serotype="O113:H21"
                     /db_xref="taxon:562"
     gene            <1..801
                     /gene="galF"
     CDS             <1..801
                     /gene="galF"
                     /function="putative regulator of UDP-Glc
                     pyrophosphorylase"
                     /codon_start=1
                     /transl_table=11
                     /product="GalF"
                     /protein_id="AAD50483.1"
                     /translation="VDKPMIQYIVDEIVAAGIKEILLVTHASKNAVENHFDTSYELES
                     LLEQRVKRQLLAEVQSICPPGVTIMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDV
                     VIDDASADPLRYNLAAMIARFNETGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSR
                     IVEFIEKPDQPQTLDSDIMAVGRYVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQ
                     SVDAMLMTGDSYDCGKKMGYMQAFVKYGLRNLKEGAKFRKGIEKLLSE"
     gene            1121..2542
                     /gene="wzx"
     CDS             1121..2542
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen repeat unit transporter Wzx"
                     /protein_id="AAD50484.1"
                     /translation="MEKNSDKLRTGVRWSAIERIITQIAQLGILLILSRILGPKAYGL
                     IGMLTIFIAISQTLVDSGFSSALIRRNKNSQKQYSTIFWFNLIVSLLIYAVIFFMSPH
                     ISAFYREKELSDILRVLSLLVIINALSIVHRARLTIMMDFKTQTKISLIAVLGSGVVS
                     VILAKNGWGVWALVVNNLVFATISTFLYFWFCKWLPDFIFQLKFFKVTAAFSVKLMIA
                     SLLNTFFDNIYQLVIGKVFYPTQVGYFTQAKNLTLLPANTYSAIIQRVTYRYFSEIKN
                     DRVRLRQKYEEVMRISAFIFFPLTISFSIFSNNIIVFLIGKAWEEAAKYISILSWCYV
                     LYPIHAISLNILNVFGRSDLFLKVEIIKKIIITVCLITTYKYGISAICMGMVFYSLLA
                     TYINTIHTNRIIKTNFIALIRIISPIAFISMLSATISFVICKFFDNNIIYIFGIMISL
                     IIYYLIYYFVDRKIVLTIWKVNK"
     gene            2544..3464
                     /gene="wbnA"
     CDS             2544..3464
                     /gene="wbnA"
                     /function="putative glycosyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbnA"
                     /protein_id="AAD50485.1"
                     /translation="MNNETLDVSVVIPVYNCGKTIKETINSILIQTGISFEIIVIDDG
                     SNDETARIVNEICDERIKYFYQNNSGISVALNNGIKRAAAKYIARIDGDDIALPTRLE
                     TQFYMLESNPDVAMVASAVEFISDDGIVIGRSFPYTSKYFIRDVLSHHNIYAHPSVMF
                     RKKTILSVGGYNSLLSGYCEDYYLWMQIIKLGKIINVSVPLTQYRISSGQITDWQPSK
                     NYYKVMNRIINSKYIDVNLVNKLAEIKQLEKEKCKNKAVNNIREEIIKKNIFNLAFNI
                     LISFRVSRKFASNTICICKNITTLMKVFLT"
     gene            3480..4667
                     /gene="wzy"
     CDS             3480..4667
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase Wzy"
                     /protein_id="AAD50486.1"
                     /translation="MNTTSVICFIVFLLNPSFGCFLFLLTLFNHEKLSIFFISLFAGI
                     LAYNLVPYDTMDLASHYMKFSELHNMSVSDIWSDGVNIFLYLCMKCLNAAGINKEWLP
                     FIATFIGYYILLNALRNNIPAYWCRKEKMICFIIWLLSISFLSLSNGIRSGLSSAFFI
                     YAVICQRNYRHLSFIIYSVLACMMHSFAIPMVILLFSMPLILKRKMLSQRFFKIAIIM
                     SVSLSFLVDINSLIVFIFNNLSFLPYIDQIYNIYIIGDRWGGGASFDTNTFIARLITQ
                     APFFYCILVLLISKRKDDFFILGAILVSVSLFLSQFWVVSERYQYVSVLLVTLHLIFN
                     NEYFLLRRKIIILLYFVIQTAVLIWGLYRFRYMILPSLEFIIYPFLFCFYNTVDMNKL
                     IKV"
     gene            4670..5722
                     /gene="wbnB"
     CDS             4670..5722
                     /gene="wbnB"
                     /function="putative glycosyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbnB"
                     /protein_id="AAD50487.1"
                     /translation="MRKKILHLVGTLDVGGIEKWVVNMINYELENKNELDFYLYSLDP
                     HKKQIVKQINLDENSITYTNKKNIFSRMYGFYCLMKKISPDVIHYHPGYSSGIYLFIS
                     KIFFKKINIVHSHSDRRSIDRRSSIFKKIYIFIMKFLINRLSDYKIAVSERAGKSLFY
                     GSFITHYCGVPDIMLSLPDIKKVSSSEFKIYHIGRNSDAKNYPFIFSIAHSLREYENV
                     HIYCMGAGLELLQKKSQEENLKNMHFLGFIENPLSHIYIHANVFIMPSLWEGLPLSVV
                     EAQKCNVPCLVSDTITKECDIGMAEFLPLDVSIWQERILNIYKEGYLMKEVNKSKFSL
                     EQDILFFKKIYRDARK"
     gene            5719..6258
                     /gene="wbnC"
     CDS             5719..6258
                     /gene="wbnC"
                     /function="putative O-acetyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbnC"
                     /protein_id="AAD50488.1"
                     /translation="MIIQIIRKIFQIFNSCFYCLFYRKLSINPIKSHFSGDVYIKNNG
                     AIFIGDYFRSRHGVTLNVNGGSINIGKNVFFNRYVSLNCMSHIFIGNNVLIGESSKLY
                     DHDHDFRSGPEEKNNKFVHQPIKVGNNVWIGSNVVILKGVTIGDNSVIGAGCIVTRDV
                     PANSVLIQKRHSTYIKIIK"
     gene            6255..7388
                     /gene="wbnD"
     CDS             6255..7388
                     /gene="wbnD"
                     /function="putative glycosyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbnD"
                     /protein_id="AAD50489.1"
                     /translation="MSVKNILFIDSYYNSLYGAQKSMINLALQLQEKNINVTIASMHS
                     GFLLEEARNNSINTYSFNFNPNILKSEHQYSGLYQKITFIFLLFFSWIRAFILHFHKL
                     KTSDVICVNDIRTFLYLFPLLMLTRKKNIWYIRIREEKRKLTFLFSFITKIIIFISSD
                     VKKNTKIRTNVLVYVLMTGFPRFNLCYKKRKDNNEIRFVTVGSLNQRKNQVETIDLFK
                     KIKHSTRSKCTLDIIGSYEPKDFCYYKELNSIISNDPELNSSVFIHGYCENILNLIED
                     YDVFLFSSLREGLPRSIIEALHAGLFVITRPVDGVNDILLYDYLGTTYTNLKNIPFEF
                     CDIADKWLTPEFRLKRNQYINEKFNNEVFVDNFLSVLDNIEIK"
     gene            7388..8566
                     /gene="wbnE"
     CDS             7388..8566
                     /gene="wbnE"
                     /function="putative glycosyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbnE"
                     /protein_id="AAD50490.1"
                     /translation="MKKIAHIQLLPMLSGVQKVTLQELMILNDNEYTKYLICKEEGEL
                     TEECKRLGIKTHVVKDLTREINAVKDIIALYKIYKFLKANDIDIVHTHFAKTGFLGRV
                     AAKLAGIPLIVHTVHGFPFDSAKNKYIAFFYKVLEFISARFADIIICLHDGDKETCKK
                     LLHVPESKVLVLPNGIAFTEFFRLSECDKKKARTILGIPESSLVFTMVGRLWEQKNPL
                     LLINAAKEVINEYPSDDIIFLLIGDGFLRKEIERIAEREIYHNKIVLLGWRKDIPDIL
                     SCSDVFVLPSRWEGMPLAILEAQATGLPCIVSDIPGNNNLVKDGVNGYLFESGSVTQL
                     KDAILRMRVIEKRHEMGINAYDYVSQKHCIHKRIDNIDNLYKKMLNKKMLNKKMKLKH
                     "
     gene            8601..9614
                     /gene="galE"
     CDS             8601..9614
                     /gene="galE"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-Glc-4-epimerase GalE"
                     /protein_id="AAD50491.1"
                     /translation="MKVLLTGGLGYIGCHIAVELLQQGHDVVIFDNLSNSHLTVLERI
                     KAITQKKVVFIRGDLRSLSDIRKTFESSQIEAVIHLAALKSVSESVRHPLEYYINNVV
                     GSLNLIQAMSHANIKRLIFSSSATVYGQPEHLPITEQASVGKVTNPYGQTKLIIEKIL
                     ADICLADPEWQITSLRYFNPIGAHPSGMIGEDPRGMPNNILPYITQVAIRRRPYFTIL
                     GDDYPTKDGTGVRDYIHVQDLALGHIAALMNAKNKSYQVYNLGTGKGYSVLDIINSFK
                     EINNIDIPFIIEKRRPGDIAECWSSPELAKRELGWQAKFELKDMLRDSWNWQQKNPFG
                     YGN"
     gene            9674..11080
                     /gene="gnd"
     CDS             9674..11080
                     /gene="gnd"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase Gnd"
                     /protein_id="AAD50492.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEESALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQGQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRSAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            11329..12495
                     /gene="ugd"
     CDS             11329..12495
                     /gene="ugd"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-Glc-6-dehydrogenase Ugd"
                     /protein_id="AAD50493.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSDRAERFAALLQEGAIKQDIPILFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNIRASSIQGIMKRIKAKCVPVIV
                     YEPAMKEDDFFRSRVVRDLDAFVQEADVIISNRMSADLADVADKVYTRDLFGND"
     gene            complement(12561..13565)
                     /gene="wbnF"
     CDS             complement(12561..13565)
                     /gene="wbnF"
                     /function="putative nucleotide sugar epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbnF"
                     /protein_id="AAD50494.1"
                     /translation="MKFLVTGAAGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFADERFDRVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKDPQWTVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            13991..>14263
                     /gene="wzz"
     CDS             13991..>14263
                     /gene="wzz"
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant Wzz"
                     /protein_id="AAD50495.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGSAAPKVSEI"
ORIGIN      
        1 gtcgacaagc caatgattca gtacattgtt gacgagattg tggctgcagg gatcaaagaa
       61 atcctcctgg taactcatgc gtccaagaac gcggtcgaaa accacttcga cacctcttat
      121 gaattagaat ctctccttga gcagcgcgtg aagcgtcaac tgcttgcgga agtgcagtcc
      181 atctgtccac cgggcgtgac cattatgaac gtgcgtcagg gcgaaccttt aggtttgggc
      241 cactccattt tatgtgcacg acccgccatt ggtgacaatc catttgtcgt ggtgctgcca
      301 gacgttgtga tcgacgacgc cagcgccgac ccgctgcgct acaaccttgc tgccatgatt
      361 gcacgtttca acgaaacggg tcgtagccag gtgctggcaa aacgtatgcc gggcgatctc
      421 tctgaatact ccgtcattca gaccaaagag ccgctggacc gcgaaggtaa agtcagccgc
      481 attgttgaat ttatcgaaaa accggatcag ccgcagacgc tggactcaga catcatggcc
      541 gtgggccgtt atgtgctttc tgccgatatt tggccggaac ttgaacgcac gcagccaggt
      601 gcatgggggc gtattcagct gactgatgcc atcgctgaac tggcgaaaaa acagtccgtt
      661 gatgccatgc tgatgacagg tgacagctac gactgcggta aaaaaatggg ttatatgcag
      721 gcatttgtga agtatggact acgcaacctg aaagaagggg cgaagttccg taaagggatt
      781 gagaagctgt taagcgaata atgaaaatct gaccggatgt aacggttgat aagaaaatta
      841 taacggcagt gaagattcgt ggtgaaagta atttgttgcg aattttcctg ccgttgtttt
      901 atataaacaa tcagaataac aacgagttag caataggatt ttagtcaaag ttttccagga
      961 ttttccttgt ttccagggcg gattggtaag acaattgatg tataaatctt ggggggggag
     1021 tggcaggtta tacctgttac atgatgaaca cgtcatagtg ctctggtagc tgttaagcca
     1081 ggggcggtag cgtgtgtttt tcatttttaa ggttagaaac ttggaaaaaa attctgataa
     1141 attaagaaca ggggttagat ggagcgctat tgagagaatt attactcaaa tagcccagct
     1201 tggcattttg cttatattgt caagaatctt aggtccgaaa gcctatggtt taatagggat
     1261 gctgacaata tttattgcaa tttcacaaac tcttgtagat agtggatttt catcagcgtt
     1321 aattaggcga aataaaaata gccaaaaaca atattcaaca attttctggt ttaacctgat
     1381 tgtttcttta ttaatatatg ctgttatttt ttttatgtcg ccgcatataa gtgcctttta
     1441 tagagaaaaa gaactttctg atatattaag agttttatca ttgcttgtga tcattaatgc
     1501 tctcagtata gtccatcgag caagattgac tattatgatg gatttcaaaa cacaaacaaa
     1561 gatttcatta atagctgttt taggaagtgg agttgttagt gttattttag ctaagaatgg
     1621 atggggggta tgggcccttg ttgttaataa tttagttttt gcaacaatat caacttttct
     1681 atatttttgg ttctgcaaat ggctccccga ttttatattt caattaaaat ttttcaaagt
     1741 aacagctgca tttagtgtga agttgatgat tgcatcactt ttgaacacgt tttttgataa
     1801 tatttatcaa ttggtaattg gaaaagtatt ttatccaact caggtaggtt actttacgca
     1861 agctaaaaat ttgaccttac ttcctgcgaa tacttattct gccataattc agagggtgac
     1921 ctatagatac tttagtgaaa taaaaaacga tagagtaagg ttaagacaaa aatatgaaga
     1981 ggtaatgcgc ataagtgctt ttattttctt tccattgacg atatcgtttt ctatattcag
     2041 taacaatatt attgtttttc tgataggcaa agcctgggag gaggctgcaa aatatatttc
     2101 aatcctatcg tggtgctatg ttctttaccc aatacatgct attagcctga atatactaaa
     2161 tgtttttggt cgatcagact tatttttaaa agtggaaatt ataaaaaaaa taataattac
     2221 agtatgttta ataacaactt acaaatatgg aatttctgca atatgtatgg ggatggtttt
     2281 ttattcattg ttagccactt atatcaatac cattcataca aataggataa taaaaactaa
     2341 ttttattgca ctcattcgaa ttattagccc aattgctttt atttctatgt tgtcggccac
     2401 aattagcttt gttatatgta agtttttcga taacaatatt atatatattt ttggtattat
     2461 gatttctttg ataatatatt acttgatata ttatttcgtt gatagaaaga tagtattgac
     2521 catttggaag gtaaataaat aatatgaata atgagactct agatgtttct gttgtaatac
     2581 ctgtatataa ctgcggaaaa acaataaaag aaactattaa ctctatttta attcagacgg
     2641 gaataagttt cgaaattatc gtaatagatg acggctctaa tgatgaaact gccagaattg
     2701 tgaacgaaat atgtgatgaa agaataaaat atttctatca aaataacagt ggcataagcg
     2761 tggcattaaa taatggaata aaaagagctg cagcaaaata tatagcaaga atagatggtg
     2821 atgacatagc tttacctact agactagaaa ctcagttcta tatgttggaa agtaatcctg
     2881 atgtagcaat ggtagcaagt gcagttgagt ttataagtga tgatggaatt gttattggaa
     2941 gatcatttcc gtatacatca aaatacttta taagagatgt gctttcccat cataatatat
     3001 atgcacaccc atcagtaatg tttagaaaaa aaacaatttt atctgttggt ggctataata
     3061 gcttattatc tgggtattgt gaggactact atttatggat gcaaattata aaacttggca
     3121 aaattattaa tgtgagtgta ccattgactc aatataggat ttcttctggt caaataactg
     3181 attggcagcc aagtaaaaat tattacaaag taatgaatag aattataaat agcaagtata
     3241 ttgatgttaa cttagtaaat aaacttgctg aaataaagca actagaaaaa gaaaaatgta
     3301 aaaataaagc agttaataat attagagagg aaataataaa aaaaaatatt tttaacttag
     3361 catttaatat attaatatcg tttcgagttt cgagaaaatt tgcttcaaat acaatttgca
     3421 tatgtaaaaa tattactacc ctgatgaagg tatttttaac ctaatactag gagtgattaa
     3481 tgaataccac atcggtaatt tgttttatag tttttttgtt aaatccatca tttggctgtt
     3541 ttttgttttt attaacattg tttaatcatg agaaattatc aattttcttt atatcgttat
     3601 ttgctgggat tctggcatat aatttagttc catatgatac aatggattta gcatctcact
     3661 atatgaaatt ctcagaatta cataatatga gcgtttctga catatggagt gatggtgtta
     3721 atattttttt atatttatgc atgaaatgtt taaatgcagc gggtataaat aaagaatggc
     3781 ttccatttat agcaacgttt attgggtatt atattttatt gaatgctcta aggaataata
     3841 ttcctgcgta ttggtgcagg aaagaaaaga tgatatgttt tattatatgg ttgttaagta
     3901 tttccttttt atctttatct aacgggatta gaagtggatt atcttctgct ttcttcatat
     3961 atgcagtaat atgccagcga aactataggc acttgtcatt tattatatat tcagtattag
     4021 catgtatgat gcatagcttc gccataccaa tggttattct attattctct atgccactta
     4081 tattaaaacg gaaaatgctt tctcaaagat ttttcaaaat tgctattatt atgtctgtat
     4141 ccttgtcttt tttagttgat ataaactcat tgattgtttt tatatttaat aacctctcat
     4201 ttctgcctta tatagatcaa atttataata tctatataat tggtgatcga tggggcgggg
     4261 gagcctcttt tgatactaac acatttattg caagattaat tacacaagct ccattttttt
     4321 attgtattct tgtgctactt atatctaaga gaaaggatga tttttttatt ttgggagcta
     4381 ttttagtaag tgtatcatta tttctttctc aattttgggt ggttagcgaa cgatatcaat
     4441 acgtttctgt tttattagta acattacatt taatttttaa taatgaatat tttcttttaa
     4501 ggcgaaaaat aataatattg ttatattttg tgatccaaac tgctgttctg atatggggac
     4561 tatatcgttt tagatatatg atactaccgt cattagaatt tatcatttat ccattcttgt
     4621 tttgttttta taataccgtt gatatgaata aattaattaa ggtctgatta tgaggaaaaa
     4681 aatattacac ttagttggaa ctttagatgt gggaggaatt gaaaagtggg ttgttaatat
     4741 gattaattat gaattggaaa ataaaaatga gcttgatttt tatttatact cacttgatcc
     4801 tcacaaaaaa caaatcgtta aacagattaa tctagatgaa aatagtataa catatacaaa
     4861 caaaaaaaat atattttcaa ggatgtatgg tttttattgc ttaatgaaaa aaatatcacc
     4921 tgatgtaata cattatcatc caggatattc ttcggggatt tatttgttta tatcaaagat
     4981 attttttaaa aaaataaata tagtgcattc tcattctgat agacgtagta ttgataggcg
     5041 gagcagcatt tttaagaaaa tatacatatt tataatgaag tttttaatta acagattaag
     5101 tgactataaa atagccgttt ctgaaagagc tgggaaatca ttgttttatg gtagttttat
     5161 aacacattat tgtggtgttc ctgatataat gctgtcacta ccggatatta aaaaggtttc
     5221 gtcatcagaa tttaaaattt atcatatagg taggaattct gatgctaaaa attacccatt
     5281 tattttctca atagctcact cattgcgaga gtacgaaaat gtacatatat attgtatggg
     5341 ggcaggttta gagctattgc aaaaaaagtc gcaagaagag aacttaaaaa atatgcattt
     5401 tttagggttt attgaaaatc cattatctca tatatatata catgcaaatg tgtttattat
     5461 gccgtcttta tgggaaggtt tacctcttag cgtcgttgaa gcacaaaaat gtaatgtacc
     5521 atgtttggtt tctgatacaa tcacaaaaga gtgtgacatt ggaatggcag aatttttgcc
     5581 tttggatgtt agtatttggc aggaaagaat attgaatata tataaagaag gatatcttat
     5641 gaaggaagtc aataaaagta aattttcctt agaacaagat attttattct ttaagaaaat
     5701 atacagagat gcacgtaaat gattattcaa ataattagga aaatatttca gatttttaat
     5761 tcttgttttt attgtctttt ttatagaaag cttagtatta atccaattaa gtcacatttt
     5821 tcaggtgatg tttatataaa aaacaatggg gcaatattta ttggtgatta ttttaggagt
     5881 cgccatggtg taactcttaa cgttaatggt ggtagtataa atataggaaa aaatgtattt
     5941 ttcaatagat atgtttcatt gaattgtatg tctcatattt tcattggtaa taacgtttta
     6001 ataggagaaa gttctaagct atatgatcat gaccatgatt ttagaagtgg gccggaagaa
     6061 aaaaataata aatttgttca tcaaccaata aaagtgggca acaatgtttg gataggtagt
     6121 aatgttgtga ttcttaaagg tgttactatt ggggataatt ctgttattgg tgctggttgc
     6181 atagttacaa gagatgttcc ggctaatagc gtattaattc aaaaacgtca ttcgacttac
     6241 ataaagatta taaaatgagc gtaaaaaata ttttatttat agacagctat tataactctt
     6301 tatatggtgc tcagaagagc atgataaatt tagctttaca acttcaagaa aagaatatta
     6361 atgtaactat agcaagtatg catagtggct ttttattaga agaagcaaga aataatagca
     6421 ttaatacata ttcatttaat tttaatccaa atattcttaa gtctgaacat caatattctg
     6481 gcctttatca aaagataact tttatttttc ttttgttttt ttcttggatc agggcgttta
     6541 ttctgcattt ccataagctt aaaacgtcag atgttatttg tgttaacgat attagaacat
     6601 ttttatattt attcccatta ttgatgctta caagaaaaaa aaatatatgg tatataagaa
     6661 ttagagagga aaaaagaaag ctgacgttct tattttcatt cataacaaaa ataataatat
     6721 ttatatcttc agatgtaaaa aaaaatacaa aaataagaac taatgttctt gtatatgtat
     6781 tgatgaccgg atttcctaga tttaatttat gctataaaaa aagaaaagat aataatgaaa
     6841 ttagatttgt tactgttggc tctttgaatc aacgaaagaa tcaagttgag acaattgatt
     6901 tatttaagaa aatcaaacat agtacaagat ctaaatgcac tctggatatt atagggtcct
     6961 atgaacctaa ggatttttgt tattataaag aactaaatag tattatatct aatgatcctg
     7021 aattgaattc atctgtattt atccatggct attgcgaaaa tatattaaat ctgattgaag
     7081 attatgatgt ttttttattt tcttcgttgc gcgaaggatt gcctcgttca attatagaag
     7141 ccctacatgc aggtcttttt gttataacaa ggcctgtaga tggtgttaat gatatactat
     7201 tatatgatta tctagggaca acttatacaa atcttaaaaa tattcctttt gaattttgtg
     7261 atattgctga taaatggcta acaccagagt tcaggttaaa gcgaaaccaa tacataaatg
     7321 aaaaattcaa taatgaagtc tttgttgata actttctttc agtattagat aatatagaaa
     7381 ttaaataatg aaaaagattg ctcatataca attgctgcca atgttaagtg gtgtacaaaa
     7441 ggttacttta caagagctta tgatattaaa tgataatgag tacactaaat atctcatatg
     7501 taaagaagaa ggggaactta cagaagaatg caaacggtta gggataaaaa cccacgttgt
     7561 aaaggatctc acacgtgaaa ttaatgctgt aaaagatata atagcgttgt ataaaatata
     7621 taaattttta aaagcaaacg acattgacat cgtacataca cattttgcta agactggttt
     7681 tttaggtagg gttgctgcaa agctggcagg gatacctttg atcgttcata ctgtccatgg
     7741 atttcctttc gattcagcaa agaacaaata tattgctttt ttttataaag tattggagtt
     7801 tattagtgcc cgttttgctg atatcataat ttgtttacat gatggtgata aggaaacatg
     7861 taagaagtta ttgcatgttc cagaaagtaa agttttagtt ttaccaaatg gtatagcttt
     7921 tactgaattc ttccgcctga gtgaatgcga caagaaaaag gctagaacta tacttggcat
     7981 tcctgaatca tcattagtgt ttactatggt ggggagactt tgggaacaaa aaaatccatt
     8041 attattaatt aatgcagcaa aagaagttat caacgaatat ccatctgatg atataatttt
     8101 tttactcatt ggcgatggat tccttagaaa agagattgag agaattgcag agagagagat
     8161 ttatcataat aaaatagttt tattaggatg gagaaaagat attccagata ttttatcatg
     8221 cagtgatgtt tttgttttac catcaagatg ggaaggtatg ccattagcaa tcttagaagc
     8281 tcaggcgact ggccttcctt gtattgtctc tgatattcca ggtaataata atttagtaaa
     8341 agatggtgtt aatggatatt tatttgaaag tggatcggtt actcaactta aagatgctat
     8401 tttaaggatg agagtgatag aaaaaagaca tgaaatggga ataaatgcgt atgattatgt
     8461 tagtcaaaag cattgtatcc acaaaagaat agataatatc gataacttgt ataaaaaaat
     8521 gttaaataag aaaatgttaa ataagaaaat gaagcttaaa cattgataat ttattaatgg
     8581 tccaattaag gtggtaactc atgaaagttt tattgacagg cggacttggt tatattggtt
     8641 gtcatattgc tgttgagcta ttacaacaag ggcatgatgt tgtaatattt gataatctta
     8701 gtaattcgca tcttactgtt ttagaacgta taaaagcgat tacacagaag aaagttgttt
     8761 tcattagagg cgacttacgc tcacttagtg acattaggaa aacttttgaa tcatcccaaa
     8821 ttgaggcagt gatacattta gccgctctga aatctgttag cgaatcagta agacatccat
     8881 tagaatatta tatcaacaat gttgttggta gtttaaattt aattcaggca atgagtcatg
     8941 caaatattaa aagattaatt ttcagctcat ccgcgactgt ttatggtcaa ccagaacatc
     9001 tccctattac cgaacaggcg agtgttggta aggtaactaa cccttatggt cagaccaaac
     9061 taattataga aaaaattctt gctgatatat gtcttgcaga tccagaatgg caaattactt
     9121 cgctacgata ctttaatcct attggtgcgc acccaagtgg catgataggt gaagatccaa
     9181 ggggaatgcc taataatata ttaccttata ttacccaagt agctatacga cgaagacctt
     9241 atttcacaat actgggtgat gattatccaa caaaagatgg tacaggtgtg agggattata
     9301 ttcatgttca agatttagca ttgggacata tagctgcatt aatgaatgca aaaaataaat
     9361 cttatcaagt atataatctc ggtacaggta aaggatactc tgttctcgat ataataaatt
     9421 cttttaagga gataaataac attgatattc catttattat tgaaaaaaga agacccggtg
     9481 atatagctga atgttggtct tctcctgagc tggcaaagag agaattaggt tggcaagcta
     9541 agttcgaact aaaagatatg ttacgcgact cttggaattg gcagcaaaag aatccatttg
     9601 ggtatggtaa ttaaaattat aatttagtaa ttaatccaga cagagaattt tatccccctg
     9661 acaggagtaa acaatgtcaa agcaacagat cggcgtcgtc ggtatggcag tgatggggcg
     9721 caaccttgcg ctcaacatcg aaagccgtgg ttataccgtc tctattttca accgttcccg
     9781 tgaaaagacc gaagaagtga ttgccgaaaa tccaggcaag aaactggttc cttactatac
     9841 ggtgaaagag tttgttgaat ctctggaaac gcctcgtcgc atcctgttaa tggtgaaagc
     9901 aggtgcaggc acggatgctg ctattgattc cctcaaacca tatctcgata aaggcgacat
     9961 catcattgat ggcggtaaca ccttctttca ggacaccatt cgtcgtaacc gtgagctttc
    10021 tgcagaaggc tttaatttca ttggtaccgg tgtttccggc ggtgaagaaa gtgcgctgaa
    10081 aggtccttcc attatgcctg gcggacagaa agaagcctat gaactggttg cgccgatcct
    10141 gactaaaatc gccgcagtgg ctgaagacgg tgaaccgtgc gttacctata ttggtgccga
    10201 tggcgcaggt cactatgtga agatggttca caacggtatt gaatacggcg atatgcagct
    10261 gattgctgaa gcctattcac tgcttaaagg tggcctgaac ctcaccaacg aagaactggc
    10321 gcagaccttt accgagtgga ataacggtga actaagcagc tacctgatcg acattaccaa
    10381 agacatcttc actaaaaaag atgaagacgg taactacctg gttgatgtga tcctggatga
    10441 agcggcaaac aaaggtacgg gcaaatggac cagccagagc gcactggatc tcggcgaacc
    10501 gctgtcgctg attaccgagt ctgtgtttgc acgatacatc tcttctctga aagatcagcg
    10561 tgttgccgca tctaaagttc tctctggccc gcaaggacag ccagcaggcg acaaggctga
    10621 gttcatcgaa aaagttcgcc gtgcgctgta tcttggcaaa atcgtttctt acgctcaggg
    10681 tttctctcag ctgcgtgctg cgtctgaaga gtacaactgg gatctgaact acggcgaaat
    10741 cgcgaagatt ttccgtgctg gctgcatcat ccgtgcgcag ttcctgcaga aaatcaccga
    10801 tgcttatgcc gaaaatccgc agatcgctaa cctgctgctg gccccgtact tcaagcaaat
    10861 tgccgatgac tatcagcagg cgctgcgcga tgtcgtcgct tacgcagtac agaacggtat
    10921 cccggttccg accttcgccg ctgcggttgc ctactatgac agctaccgtt ctgctgttct
    10981 tcctgcgaac ctgatccagg cacagcgtga ctatttcggt gcacatactt ataagcgcat
    11041 tgataaagaa ggtgtgttcc ataccgaatg gctggattaa tctgatttaa atcaactaat
    11101 caatgcaagg cccggagaaa ccctccgggc ttttttatta tacaaagcgg caggttaggg
    11161 ccttttttta taatttatag ttaaaaacgc gatataatac agagccgcac agcaggatcg
    11221 ctgccttgac agttcatcta catcagcgtt aaaaatcccg cagtagatga agttgtggtg
    11281 gtggattaat gaccactcta aatgtttaac cggaagaagt cagagctaat gaaaataaca
    11341 atttcaggaa caggttatgt tggtctttca aatggtattc tgattgcgca aaatcacgaa
    11401 gtggttgcgc tggatatcgt tcaggccaaa gtagacatgc ttaacaagaa gcagtcaccg
    11461 attgttgata aggagattga agagtatctg gcgactaaag atctcaattt ccgcgctacg
    11521 acagataagt atgacgcgta taaaaatgcc gattatgtta ttattgccac gcctaccgat
    11581 tatgacccga aaacaaatta ttttaatacc tcaagcgtgg aagcggtcat tcgcgatgtg
    11641 acagaaatta atcccaacgc ggtaatgatt ataaaatcaa ctatccctgt tggttttaca
    11701 gagtccatta aagaacggtt tggtattgaa aatgtgatct tttcgccgga gtttttacgc
    11761 gaagggaaag cactttatga taacttgcat ccatcacgca ttgtgattgg tgagcaatcc
    11821 gatcgcgctg aacgttttgc agcgttatta caggaaggcg ctattaagca agacatacca
    11881 atattgttta ctgactcaac cgaggctgag gcgattaaac tttttgccaa tacttatctg
    11941 gcgatgcgtg tggcttattt taatgaacta gatagttacg ctgaaagtct gggtcttaac
    12001 tcccgccaaa ttattgaagg ggtttgcctt gatccgcgta tcggtaatca ctacaacaat
    12061 ccctcatttg gttatggtgg ttattgtctg ccgaaagata ccaagcagtt actggcaaac
    12121 tatcaatctg taccgaataa tctgatttcg gcaattgttg atgctaaccg cacgcgaaaa
    12181 gactttattg ccgattctat ccttgcacgt aaaccgaaag ttgttggcgt ttatcgtctg
    12241 attatgaaga gtggttcaga caatatccgt gcttcctcga ttcagggtat tatgaagcga
    12301 atcaaggcga aatgtgtgcc agtaatcgtt tatgagccag cgatgaaaga ggacgatttt
    12361 ttccgttcgc gcgtggtacg tgatctggat gcgttcgtac aagaagctga tgttattatt
    12421 tctaaccgta tgtctgccga tctggctgat gtagcagata aagtttatac gcgcgacttg
    12481 tttggcaatg attaattatt ttgttttatt ctaagaaaag gccctaatat attagggcct
    12541 tttcttatgg ttttgtaaaa tcaaacttta tagaagttac gataccattc tacaaagttc
    12601 tttaccccct ctttaactga cgtttcaggt ttgaatccta ttacgtcata cagtgctttt
    12661 gtatcagcac tggtttccag tacatcaccg ggttggagag gcatcatatt cttgttggct
    12721 tcaataccca gagcctcttc taaagcattg atatagtcca tcaactccac aggcgaacta
    12781 ttaccaatgt tatagacacg atatggtgct gaacttgttg caggcgatcc agtttctaca
    12841 gtccactgtg gatctttttc tggaataaca tcctgtaagc gaataatagc ttcggcaata
    12901 tcatcaatat aagtaaagtc acgcttcatt ttgccgaagt tgtaaacatc aatgctttta
    12961 ccttccagca tggctttagt aaatttaaat aatgccatat ccggacgtcc ccatggacca
    13021 taaaccgtaa agaaacgcag ccctgtggtc ggtaagccat acaaatgaga atacgtatgg
    13081 gccatgagtt cattcgcttt tttagttgct gcataaagcg aaacaggatg atctacagag
    13141 tcatctgtag agaaaggcat cttgcggttc atgccataaa cagaactgga ggaagcgtaa
    13201 agtagatgct gaacattatt atggcggcat ccttccagta tgttcaggaa tccaatcagg
    13261 tttgcatctg catatgcatt gggattttca agggagtaac gtacaccggc ttgcgcagcg
    13321 aggtttatta cgcgatcgaa ccgctcgtct gcaaacagtg ccgccatttt ctcgcgatcg
    13381 gccaggtcaa ttttataaaa actgaaattg tcgtgcttga gtaaatcaag tcgtgcttgt
    13441 ttgaggttga catcgtaata atcatttaag ttgtcaatcc ctacaacctg atgaccagct
    13501 gcaagaagcc gtttacttac atagaaaccg ataaagccag cagctcccgt aaccagaaat
    13561 ttcatttata atcctcactc aggctagaat atagccaatc ttcatctggc ataactgaaa
    13621 gttaaatcat accgttagac aagaaaaaaa gataatcggt atcggttcta aacctggctg
    13681 ttttttctgg taacgtgctc attttacaat caaagctgtt ctaaactgac tattagacca
    13741 ccttcaaaca acaagccgac gtcattatct ccaaccgtat ggcagaagag cttaaggatg
    13801 tggcagacaa agtctacacc cgcgatcttt ttggcagcga ctaacatcat gttatcaggg
    13861 cgattttcgc cctgattctc ttatgttccc tttgtaataa ttcattattt ttatcattta
    13921 tcctatagca ttcatggcga ttatcgctaa actatggcga cttaaaatta tttcgtccgt
    13981 tagggtaatg atgagagtag aaaataataa tgtttctggg caaaaccatg acccggaaca
    14041 gattgatttg attgatttac tggtgcagtt gtggcgtggc aagatgacaa ttatcatctc
    14101 tgtcattgtt gctattgccc tggctatagg gtatctggcc gttgccaaag aaaagtggac
    14161 ttccacagct atcgttactc aacctgatgt gggacaaatc gctggttata acaatgccat
    14221 aaatgtgatt tatggctcgg cagcgccgaa agtgtctgag atc
//