LOCUS       EU296405                8392 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Escherichia coli serogroup O112ac O-antigen gene cluster, complete
            sequence.
ACCESSION   EU296405
VERSION     EU296405.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 8392)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O112ac"
     misc_feature    41..8245
                     /note="O-antigen gene cluster"
     gene            41..844
                     /gene="wfeJ"
     CDS             41..844
                     /gene="wfeJ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeJ"
                     /protein_id="ACD37012.1"
                     /translation="MEPFSVLMSLYIKEKPEYFEECLISLKDQTLMPNEIIIILDGQI
                     TTALKSVIAKWKKCLPIKIISLDKNVGLGNALKLGLKNCTYRLVARMDTDDICHPQRF
                     EKQILQFENDNDLVLVGGNICEFNEDFSIKISKKRVPISPQDIKKYAQFRNPFNHMTV
                     MFDRYKIIDAGSYSHHLFMEDYNLWLRCLAKNYKMINLTEDLVYVRGGISMIRRRKGI
                     NYVKSEIQLYNIKKRLKIVSGIKGIKILIFRVLPRLMPQSLLFMIYKNR"
     gene            846..1883
                     /gene="wfeK"
     CDS             846..1883
                     /gene="wfeK"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeK"
                     /protein_id="ACD37013.1"
                     /translation="MKIAILVNSLEIKGPVIVAVNLAKQLYEAGEDVTLLYLNDKVER
                     IDTYGIKIKKINLVSFYILREFDIIHSHSLAPDLLNAFQRVIFKNRSITTVHNNIYTD
                     LYPEYTKLKSIILKSLWQISWWFIDEKICISKSLLNQYDNAKKKGWDYVYNSIRDDNN
                     LGLNDFDLNAINVLKKIKREGKIIVGSCAVITERKGLQHLVSAARFLPDKYRIVIVGD
                     GPYKVNLMKHSAENIIFFERTLSAKLYMRYFDIYAMTSLSEGFGLAAVEAALEGCHLI
                     CSNIDTFKELFGENCSYFELEKPSSLVDAIENAELKTGNKLLSYVREKFSGENMLKGY
                     LRIYYSNDKKK"
     gene            1867..2952
                     /gene="wzy"
     CDS             1867..2952
                     /gene="wzy"
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ACD37014.1"
                     /translation="MIKKNNIYRFYILVCVFSGFYIFGFFPAPYYLCLILGFVFLSLS
                     KIKHVDKNILFLLCIVIIYMVFMLFNSRINVWSYYFIGLLTYFIIILLRNNLDYYQKI
                     AIVRKTVLISLLLVILDTIYRFYYPREEYLLAIEQQGKIDYLFYGYKHSFLFQDSNFV
                     GLYILSVFFLFRDNMSIFKWKKSILLTIIILIILSLSRAAIVALILTECIRMLCGMKV
                     DVRLKIVSLIFMAIPTIYLLFVLVANIDDTSFESKFMLLNKFLTTFQERNFTELLFGW
                     GLDNTREHWGIAAHSLFNTLLLEMGLVGFIIITISIVYCWCLNTKTLYHFMGLSIAFL
                     SFGLIFTPFFIPLGLNIIMRDVSNEKL"
     gene            2930..3991
                     /gene="wfeL"
     CDS             2930..3991
                     /gene="wfeL"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeL"
                     /protein_id="ACD37015.1"
                     /translation="MCRMKNYDIIFISGSHQIYGGGQVFIDEINKKLSSQGIKSIILA
                     SDDIFDNSLNIPRVDTWKYKIKNYKKLLEILNEIDCNRKAKIITNDITLSMLAVILRF
                     NGWKIYPLIHMSLYNTSSNYKFIKFLYPYIRGFLIRMASEKILSVNKENDRILGKKVC
                     YIGNFTVNKQDSVQSINVDKNIDLLYVGRFDIEKRPDRFINIVQYLLQRGRPVRATMV
                     GSGKYYDQIRKYVHENNLSDYINLMGFLDKEKIIPLYKRSRMLVITSKTEGLPTVILD
                     AAVYGVNFISPPIGSIPYLHKNWNVGHIVDIHNMGEYIDQRWDILINKVSDMQLTMFA
                     QEHFIDSFILKFMDSLYEG"
     gene            3981..4865
                     /gene="wfeM"
     CDS             3981..4865
                     /gene="wfeM"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeM"
                     /protein_id="ACD37016.1"
                     /translation="MKVKYSIVVPLYNKSSYIKDTLESICHQLTIDTEVIIIDDGSTD
                     DSVSIVEEFIHKYQQIKLFCFENKGVSEARNRGLKNANGDYVIFVDADDIINEGYVND
                     IIKTVNTIPEQFDILGYNYIRGGVPVIPNHVKDGYINYFELYLKYGPPFCSSSVVIAK
                     NKLSDLQLFPEGEWLGEDIYAWAKIIMGGGKVYFRNFNACNYITDINGAMAKQKTKIK
                     LIRKDNEIKIDDPKFDLFINYHKRDYLKSCLIFGDKNSLRQYIKNESSANILFYKILS
                     YLPHALFKFLVCVKRKML"
     gene            4862..5791
                     /gene="wfeN"
     CDS             4862..5791
                     /gene="wfeN"
                     /note="pyruvyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeN"
                     /protein_id="ACD37017.1"
                     /translation="MTTLLFGYFDLNFGDDWLIHEFINQYHKDNVILVVSDENLYLPF
                     KHDIRFRKVSDLGKIKAFLQSDEICIVGGSMFQDSKHWFGHYLKLYIYLLIARILGKK
                     NYVIGCNLNAIKNKKLNMLLKLLYKQVDHFRVRDSNSVKILNKDYNINEALISVKPDL
                     ADKSKLSLTVTKENSCAVSIINNKNTGKKKYYDWLTSNFKELRRKGIYHFKLFGFDSG
                     AESDEKSINDFLVLKRHLFADCTFTIIIYNGNVDEFIMKWSTSKYALCTRFHSYILAR
                     NCKQNFRVFCYSNKIVEYIKTHSPFEMEYVDVA"
     gene            5775..7202
                     /gene="wzx"
     CDS             5775..7202
                     /gene="wzx"
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ACD37018.1"
                     /translation="MSMLLNLIIPIISFGIVSIMGLIMTPFFINNFGYEGLGLISNSN
                     ALIQSLFFAGGAFTTVYSREYSNAVNNIEKSVLLRELNNILTAIFFIAVLLSIVFYIF
                     YIFKDNFSYNYYALVFLASAIIIISQSFNVFPFAANRLYINSVIDCARTLLRNILCMA
                     LFYLSIDNVLSNGWAMILSAVITLLVLKLVFKENKVFYVGRMSNNYNIKNSFWVCINQ
                     GGAYIFSFADVFLINLMLGHYIGGSYAVLIQIPVLLKSVSVVAIGSISSFTVKVHQRF
                     EKDRTIIKKIINKVVVFFSFFISLAFILVDLYKESFFSIWLPTAVTSSMLKQFNYICI
                     IMMLLSISSLVNIFLVAWGHFIIPAKATVFSAVVSVLVVVLLYFFNYKLSEMIIFSVI
                     YLMLILKNLICYLYFFMQSGLSLIHFIFSLILSFCVITINFLVAKSIRYYIESDFLSI
                     VILFFVMMTISLPLLWDFKKLREYI"
     gene            7223..8245
                     /gene="gne"
     CDS             7223..8245
                     /gene="gne"
                     /note="GlcNAc-4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gne"
                     /protein_id="ACD37019.1"
                     /translation="MNVLVTGGLGYIGSHIVVDLLNSQFKVAVVDNLSNSNINVKNKI
                     EFLTKRKVKFYECDVCDNESINKIFNNNDFYAVIHLAGLKSVAESIQEPVRYYANNLE
                     GTLCVIKNSIKYNVKKFVFSSSATVYGLPERIPLDENCQVGKTINPYGTSKFFSEQIL
                     HDIANANSQIDITILRYFNPVGAHSSMSIGEDPRGIPSNLVPYITKVAAGKLPFLSIF
                     GGDYDTKDGTGVRDYIHVMDLAKGHIAALRMAADSSKSNFHIYNLGTGKGYSVLELVN
                     TFETITGKKINYKICDRRKGDVAECWSDPSLALEELHWKAEKDLSDMLKDAWAWEIRD
                     VIPQEE"
ORIGIN      
        1 attggtagct gtaagccaag ggcggtagcg tggggaaata atggaacctt tttctgtact
       61 aatgtcttta tacattaaag aaaaaccaga atattttgaa gagtgtctta taagtctcaa
      121 agatcaaact ttgatgccaa acgagatcat tattatatta gatggtcaaa ttactacagc
      181 attaaaatcc gttatagcta aatggaaaaa atgtttacct ataaaaataa tttcattgga
      241 taaaaatgtt gggttgggta atgctctaaa attaggtctg aaaaattgta cctatcgttt
      301 ggttgccaga atggacaccg atgatatttg tcatcctcag cgattcgaaa aacaaatttt
      361 acagtttgaa aatgataatg atctcgtgct tgtaggcggc aatatctgcg aattcaatga
      421 agacttcagt atcaaaatta gcaaaaaaag ggtgccaatc agccctcaag atattaaaaa
      481 atatgcacaa tttaggaatc cttttaatca tatgactgtt atgtttgata gatataaaat
      541 tatagacgca ggaagctata gtcatcattt atttatggaa gattataact tgtggttacg
      601 ctgtctcgct aaaaattata aaatgataaa tttaacagaa gatttagttt atgttagagg
      661 cggtatttct atgataagaa ggcgcaaagg tataaattac gtaaaaagtg aaattcaatt
      721 atataatatt aaaaaaagat taaaaattgt aagtggtatc aaaggaataa agattctaat
      781 ttttagagta ttacccagat taatgcctca atcgttgtta tttatgattt ataaaaatag
      841 ataaaatgaa aattgcaatt ttagtaaaca gtttagaaat taaagggcct gtcatagttg
      901 cagttaattt agcaaagcaa ctatatgaag cgggcgaaga tgtgacatta ttatatctga
      961 acgataaagt agaacggatt gatacatacg gaataaaaat aaaaaaaata aatcttgtaa
     1021 gcttttatat acttcgagaa tttgacatca ttcattctca ctcattagca ccagatttat
     1081 taaatgcatt tcaaagagtt atttttaaaa ataggagcat aaccacagtt cataataata
     1141 tatacacgga tctctatcca gaatacacta aactaaaaag cataatactt aagtccttgt
     1201 ggcaaataag ttggtggttc atagatgaaa aaatttgtat aagtaagtca ttacttaatc
     1261 aatatgataa tgcaaaaaaa aaaggttggg actatgtata taattcaata cgtgacgaca
     1321 ataatttagg actgaatgat tttgatttga atgcaattaa tgtactaaaa aaaataaaga
     1381 gagaaggtaa aattatagta ggatcatgtg cggttattac agaaagaaaa ggcctacaac
     1441 atttagtaag cgctgccaga ttcttaccgg ataaatatag aattgtgata gttggagatg
     1501 gcccttataa agtaaattta atgaaacata gcgctgagaa tataatattt tttgagcgaa
     1561 cattatcagc taaactatat atgcgttact ttgatatata tgctatgact tctttaagtg
     1621 agggttttgg tcttgctgca gttgaagcag cgttagaagg ttgtcattta atatgtagta
     1681 atattgatac gtttaaagag ctgtttggtg aaaactgtag ctactttgaa ctcgaaaagc
     1741 ctagtagttt agttgatgct atcgaaaatg ctgaattaaa gactggaaat aaattattat
     1801 catacgttag agaaaaattc agtggtgaaa atatgttaaa aggttactta aggatatatt
     1861 actcaaatga taaaaaaaaa taatatttat agattttata ttttagtatg tgtatttagt
     1921 ggtttttata tatttggatt ctttcctgcc ccatactatc tttgtttaat attggggttt
     1981 gtgtttttaa gccttagcaa aatcaaacat gttgataaaa atattttatt tctgttatgc
     2041 attgttatta tctatatggt ttttatgttg tttaactcta gaattaatgt ttggagttat
     2101 tattttattg gccttctgac ttattttatt attatcttac taagaaataa tcttgattat
     2161 tatcaaaaaa ttgcaattgt gagaaagact gtcttaatct cattgttact tgtcatattg
     2221 gatactatat atagattcta ttatccgcga gaagaatatt tacttgcaat agaacaacaa
     2281 gggaaaattg attatctctt ttatggatat aaacatagct ttttattcca agacagtaat
     2341 ttcgtaggtt tatatatttt gtcagttttt tttctgttta gggataacat gtctatcttt
     2401 aaatggaaaa aaagtatcct attgacaata ataatattaa ttattttatc cttatcaagg
     2461 gcggcaatag tcgcattgat tttaaccgaa tgtatacgta tgttatgtgg catgaaagtt
     2521 gatgtacgat tgaaaattgt gtcattaata tttatggcca taccgacaat atatctctta
     2581 tttgttttgg tagctaatat tgatgacaca agttttgaat cgaaattcat gctattaaat
     2641 aaatttctta ctacttttca agaaagaaat tttactgaat tattatttgg atggggctta
     2701 gataatacac gagaacactg gggaatagct gctcatagtc tatttaatac attacttctg
     2761 gaaatgggat tggtgggatt cattataatt accatatcaa tagtatattg ttggtgctta
     2821 aacaccaaaa cgttatatca ttttatgggg ttaagcatag catttctttc atttggtttg
     2881 atttttactc cattttttat tcctcttggc ttaaatataa ttatgcgaga tgtgtcgaat
     2941 gaaaaactat gatattattt ttatctcagg atcacaccaa atatatggag gaggacaggt
     3001 atttatagac gaaattaata aaaaactatc atcgcaaggg attaaaagta taattttagc
     3061 atctgatgat atatttgata attcgttaaa tataccaaga gttgatactt ggaaatataa
     3121 aataaaaaat tataaaaaat tactggaaat tcttaatgaa attgactgta accgcaaggc
     3181 aaaaataatc acaaatgaca taacgttatc gatgcttgcc gttatactaa gatttaacgg
     3241 ttggaaaata tatcctctta tacatatgag tctgtataac acatcgagca attataaatt
     3301 tattaagttc ctatatcctt atatccgagg ttttctgata cgtatggcct cagaaaaaat
     3361 tttatctgtt aacaaagaaa atgatagaat cttgggtaag aaggtatgtt atattggaaa
     3421 ttttacggtt aataaacaag actcggttca atctattaat gtcgataaaa atatagatct
     3481 attatatgtt ggccgttttg atatagaaaa acgtccagat agatttatta acattgttca
     3541 atatttgtta caaagaggac gtcctgtcag ggccacaatg gttggcagcg gaaaatatta
     3601 tgatcaaatt agaaaatatg tacatgaaaa taatcttagt gattatataa atttaatggg
     3661 ttttctagat aaagaaaaga tcattccatt gtacaaaaga agtagaatgt tagttattac
     3721 aagcaaaact gaaggcttac ctactgtaat tctagatgca gctgtgtatg gagttaattt
     3781 tatttctcct cctataggca gtattcccta tttacataaa aattggaatg ttggacatat
     3841 tgttgatatt cataatatgg gggagtatat tgatcagcga tgggatatat taattaataa
     3901 agtatccgat atgcaactca cgatgtttgc tcaagaacac tttattgata gttttattct
     3961 taagtttatg gatagtttat atgaaggtta agtatagcat cgtagtcccg ttatataata
     4021 aatctagcta tataaaagat actttagaaa gtatatgtca tcaattaact attgatacgg
     4081 aggtaataat tattgatgat ggctcaactg atgattcagt ttcaattgtg gaagaattta
     4141 tacataagta tcaacaaata aaattatttt gttttgaaaa taaaggggtg tccgaggcaa
     4201 gaaatagagg gcttaaaaat gcaaatggag attatgtaat atttgtagat gctgatgata
     4261 ttatcaatga aggctatgtt aatgatataa taaaaacagt taatactatt ccggagcaat
     4321 ttgatatatt agggtataat tatattcgcg gtggagtacc agtaatccca aaccatgtta
     4381 aagatggtta tataaattat tttgaattat accttaaata tggccctcct ttttgttcat
     4441 cttcagttgt tatagcaaaa aataaattgt ccgatctaca gctatttcct gaaggagaat
     4501 ggctgggaga agatatttat gcgtgggcaa agataattat gggggggggc aaagtatatt
     4561 ttagaaactt caatgcttgc aattatatta cagatataaa tggtgcaatg gctaaacaaa
     4621 aaaccaaaat taaattaata agaaaagata atgaaataaa aattgacgac cctaaatttg
     4681 atttgttcat caattatcat aaacgagatt atcttaaaag ttgtttgata tttggagata
     4741 aaaactcact tcgccagtat atcaaaaatg aatctagtgc taatatattg ttttataaaa
     4801 ttttgtctta tttacctcat gcattattta aattcttagt gtgtgtaaaa aggaaaatgt
     4861 tatgaccacg ctattatttg gctattttga tttaaatttt ggtgatgatt ggttaattca
     4921 tgagtttata aatcaatatc ataaagataa cgtaatatta gttgtttctg atgaaaatct
     4981 ttatttgcct tttaaacatg acattagatt cagaaaagtg agtgatttag gtaagattaa
     5041 agcttttcta caaagtgacg aaatatgtat tgtcggagga tcaatgttcc aggacagcaa
     5101 acattggttc ggtcattatt taaaattata tatttattta ctgattgcta gaattttagg
     5161 gaaaaaaaat tatgtgattg gctgtaactt aaacgccata aaaaataaaa aactcaatat
     5221 gttattaaaa ttattatata agcaagttga ccatttccgt gtgcgagatt caaattcagt
     5281 aaaaatatta aacaaagatt acaacataaa cgaagcattg atttctgtaa agccagatct
     5341 tgctgataaa agtaagttat cattaacagt tacgaaagaa aacagttgtg cagtgtcaat
     5401 aatcaacaat aaaaacaccg gtaaaaaaaa atattatgat tggttgacga gtaattttaa
     5461 agaattaaga agaaaaggaa tatatcattt caaactattt ggtttcgatt ctggggcaga
     5521 gtcagatgaa aaatcaatta atgattttct tgtattaaaa cgtcatttat tcgctgattg
     5581 tactttcaca atcattatat ataatggcaa tgtagatgaa tttataatga aatggagtac
     5641 aagtaaatat gctttatgta cgagatttca ttcatacata ttagcacgga attgcaaaca
     5701 gaatttcaga gtattctgtt attctaacaa aattgtcgaa tatattaaaa cgcattctcc
     5761 gtttgaaatg gagtatgtcg atgttgctta atttaatcat tccgattatt tcctttggaa
     5821 ttgtgtcaat tatggggttg attatgaccc catttttcat taacaatttt ggctatgaag
     5881 gtttgggatt aatctctaat tcaaacgcgc ttattcaatc tctctttttt gctggaggag
     5941 cgttcactac tgtttactct agagagtatt ctaacgcagt taataatata gaaaagtctg
     6001 tccttttgcg cgaattaaat aatatattaa ctgctatatt ttttatagcg gtattactta
     6061 gtatagtatt ctatattttt tatattttta aagataattt tagctacaac tattatgcat
     6121 tagttttttt agcatcagcc ataattataa tatcacaatc atttaatgtg tttccttttg
     6181 ctgcaaacag gctttatata aactcagtta ttgattgcgc cagaacatta ttgcgtaata
     6241 tattatgtat ggctttattt tatctaagca ttgataatgt actgtctaat ggctgggcaa
     6301 tgatattatc cgcagtcatc acattattag tcctgaaact agttttcaaa gaaaataaag
     6361 ttttttatgt tgggagaatg tccaataatt ataatattaa aaactcattt tgggtgtgta
     6421 tcaatcaggg gggggcttat attttctctt ttgctgatgt attcttgata aatctaatgt
     6481 taggtcatta cattggaggt tcatatgcag tgctaataca aataccagta ttgttaaaat
     6541 ctgtttctgt agtggctata ggaagtatat cttcttttac cgtgaaggtt caccaaagat
     6601 ttgaaaaaga taggacaata ataaaaaaaa ttataaataa agtggtggtc tttttctctt
     6661 tttttatttc ccttgctttt attttggttg atctttataa agaatctttt ttttccattt
     6721 ggttacctac cgctgttacg tcatcgatgt tgaaacaatt taactatata tgtattatca
     6781 tgatgttact ttctatatca tctttggtaa atattttttt agttgcttgg gggcacttta
     6841 taattccagc taaagctaca gttttttctg ctgtggtatc agttttagtt gtggttttat
     6901 tatatttttt caactataaa ctatctgaga tgattatttt tagtgtaatt tatctgatgt
     6961 taattttaaa aaatctgata tgctatttgt atttctttat gcagagtggt ttgtcattga
     7021 tacattttat ttttagtctc atactcagtt tctgcgtaat aactatcaat ttccttgtgg
     7081 ctaaaagtat tcggtattat attgaaagtg attttttgtc gattgtaata ttatttttcg
     7141 tgatgatgac tatatctcta cccttgctct gggattttaa aaaattacga gaatatattt
     7201 aacaagttaa taagaggtct ttatgaatgt actggttacc ggtggtttag gttatattgg
     7261 ttctcatatc gttgtggatt tattaaattc acaattcaag gttgcagttg ttgataattt
     7321 atctaattct aatattaatg tcaaaaataa aattgagttt cttacaaaaa gaaaagtaaa
     7381 attttatgaa tgtgatgttt gtgataatga atcaattaat aaaatattta ataataatga
     7441 tttttatgct gtaatacatt tagctgggct gaaatctgtc gctgaatcta tccaagaacc
     7501 tgttaggtat tacgcaaata acttagaagg cacactatgt gttattaaaa actcaataaa
     7561 atataatgtc aaaaaatttg tttttagttc ttctgccact gtatacggcc tacctgaacg
     7621 aatacctctt gatgaaaatt gtcaggttgg taaaacgatt aatccttacg gcacttcaaa
     7681 atttttttct gaacaaattc ttcacgatat tgctaatgca aactctcaga ttgatatcac
     7741 aatattaaga tattttaacc ctgttggtgc tcatagttcg atgagcatag gtgaagaccc
     7801 cagaggtata cctagcaatt tagtcccata catcacaaaa gttgcagccg gaaaactacc
     7861 attcttatca atatttggcg gagattatga tactaaagat ggaactggcg taagagacta
     7921 tattcatgtc atggatctcg ccaaaggcca tatagcagca ctgcgaatgg cagctgatag
     7981 tagtaaatcc aatttccata tatataattt ggggacaggt aaaggatatt ccgttcttga
     8041 attggtaaat acctttgaaa caataacagg aaagaaaata aactataaaa tatgtgatag
     8101 aaggaaaggt gatgtggctg aatgctggtc agatccttca cttgctttag aagagttaca
     8161 ctggaaagcc gaaaaagatt taagtgatat gttaaaagat gcttgggctt gggaaatccg
     8221 agatgtaata ccgcaggaag aataatatta tggctgtgtg ttaaaaacta cctttatatt
     8281 cattcttaac ttattataat actgcattta taccgttagc aaagtacggt gtctatccct
     8341 gacaggagta aacaatgcca agcaacagat cggcgtcgtc ggtatggcag tg
//