<pre>LOCUS       EU694095               10884 bp    DNA     linear   BCT 12-JAN-2009
DEFINITION  Escherichia coli serogroup O107 O antigen gene cluster, complete
            sequence.
ACCESSION   EU694095
VERSION     EU694095.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10884)
  AUTHORS   Wang,Q., Perepelov,A.V., Feng,L., Knirel,Y.A., Li,Y. and Wang,L.
  TITLE     Genetic and structural analyses of Escherichia coli O107 and O117
            O-antigens
  JOURNAL   FEMS Immunol. Med. Microbiol. 55 (1), 47-54 (2009)
   PUBMED   19040662
REFERENCE   2  (bases 1 to 10884)
  AUTHORS   Wang,Q., Perepelov,A.V., Feng,L., Knirel,Y.A., Li,Y. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2008) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P. R. China
FEATURES             Location/Qualifiers
     source          1..10884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O107"
     misc_feature    1..10884
                     /note="O antigen gene cluster"
     gene            107..1192
                     /gene="rmlB"
     CDS             107..1192
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ACH97127.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNNEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            1192..2091
                     /gene="rmlD"
     CDS             1192..2091
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ACH97128.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRMALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLKAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            2149..3027
                     /gene="rmlA"
     CDS             2149..3027
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ACH97129.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAFRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGD"
     gene            3032..3574
                     /gene="rmlC"
     CDS             3032..3574
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ACH97130.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQRQFEEIVGRKIS
                     FVQDNHSKSKKGVLRGLHYQLPPFAQGKLVRCINGEVYDVAVDLRPESINYGKWVGII
                     LSAENKKQLWIPEGFAHGFLVISEFAEFAYKTTNYYSKESERGIRWNDPTINIQWPKM
                     GQYLLSPKDALAPFIELRID"
     gene            3574..4791
                     /gene="wzx"
     CDS             3574..4791
                     /gene="wzx"
                     /note="O antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ACH97131.1"
                     /translation="MENKKNHIYGIVNNIIVMLSPIIIIPFAIKNVGIDEYGYFVQVN
                     IIYSCLISIFTASLSGYFIKSYLEKKLSFHDIFIIQLFINLFSTAIIGIYILLRFDVV
                     APYIFVVAMLTNTLNFEWFFHAIGAQKQLLIRNLIIKTMFVIIAIFILEVYKNIAVYF
                     VIFGLNIIIANFLLGIYSFTKIKNKEYILFSNGRGGNRLFCFIKDAKFFFLSPTIGAV
                     YQYGDQILVSLLFPKTALVFINLAKQIIGASVMVSGTLCRVEQKNIFSSEKIERLTKI
                     KKTLMAYVFYLMTTSILIILIGPLILGMLIKETVSLGLNYYILIAGIYVFTSLSIFID
                     YIIGLTFRTEHYTSISNICCATIVTLLNAMFLEKYGATFSLFSLFIGELLVFILLSTM
                     HYINIKRRTYYNG"
     gene            4784..6106
                     /gene="wzy"
     CDS             4784..6106
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ACH97132.1"
                     /translation="MVNILLCINIIILLICICIYLIALKSKKAPRLFALYLGAFILFI
                     ESHIILAITTSFNFGTSEWFFNGEFDYNTKTEVITSINLFIIGMILGSVFIASTITYK
                     SSSYDVTFENKSIARFSWLLLVSILPFVVVYLINLIAFISSNGFYSLYINGNKISGGY
                     ILDLFFLTLYSLLISLKNKKKILFIILCVACVYLFIGTRLEFMFKVFPVLIYYILISK
                     NIHKYFRLKNILAISILFWGLIFSMQYSVSARDNIEMGSNIITTFLKQQGVSVNVIGI
                     AIKDKNNSLLSESVILSPLYDSAISLANSLVGVQSNGNSVEFAENSFSLSHKLSYLED
                     PSAYLAGYGVGGAAIAELYIVGGYLACLIGGMLTYIFISILEKIAKKSFFNFIFVMLI
                     TGKILYSPRGEFLSFMSADRMLILFLIFTFSYKFLLATSNKKMSFKNE"
     gene            6099..6899
                     /gene="wclW"
     CDS             6099..6899
                     /gene="wclW"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WclW"
                     /protein_id="ACH97133.1"
                     /translation="MNELVSIIIPLYNQGEYIEETLNSVKQQSYSNYELIIVDDGSND
                     QFTIEKIKELRFKGYTIISKQNGGLASARNEGIRNAIGKYIIVLDSDDIIHRDYIKNL
                     LEVITNTKARIVYSKALLFEYRTGMWMLPSFTMRRMLKSNIIFCSAMYYREDWFNVGG
                     YDESLRTGLEDWDFWLSLIGLYNNHNDIVARVNKPLFFYRIRRNSMLRSINYTEKEKI
                     VNYIHKKHIQLFELYKVKPLIYSKQNLISKIFNKIASKICGIGYNVKR"
     gene            6886..7830
                     /gene="wclX"
     CDS             6886..7830
                     /gene="wclX"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WclX"
                     /protein_id="ACH97134.1"
                     /translation="MLNDKKEMVAIVLASYNGQLYIKEQVLSILNQSYQYIKLYCSDD
                     GSTDGTIDYLKEYQKNNIVIAKNDGKKGPAYNFINGLKLVGDEKYIIFSDQDDVWMPE
                     KVSTLKSYADKLLNNDKPGAIYCDALIVDHSLTQLGKRMYGEHHICPTKISELLYLNG
                     GVQGASMLINRKMMDEMLSYNKYIYMHDQLATYLAVVYKNIYYINIPLMLYRQHSKNV
                     IGVNTNKIEKMKLLFSSNMIDSRSYKFITEFSQHYACNNHINSRNSLEYYIELKENIL
                     KSIIKEFLNGNATLHNSRSKLLIKLFFKFIINKVVQKK"
     gene            7827..8888
                     /gene="wclY"
     CDS             7827..8888
                     /gene="wclY"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WclY"
                     /protein_id="ACH97135.1"
                     /translation="MKIAYVVSSKKKCGPNIVILNIVKELANKHEMEIFFLDESDDDV
                     FECVNVKSTQIKKASDLKEHLKRFDIIHSSGIRPDALVVLCKVIYRVKCKIITTIHNY
                     VFQDLYYSYGLVKSLIWGLLWCSIWLFFDKLVILSKNADNYYWFLPSAKKNIIYNGID
                     DNDCLQNKKCNYRKEFNIPDDGILAGSCANLTKCKGIDLVIQTLTKEHKIYYIVAGDG
                     IEKHNLINLVKARKLHERVYFIDFLDEPESFMSQLDVFLMPSRSEGFGLTVLESTKLG
                     IPVITSNIPIFMELFDQMCLTFDIKNPSTLIDVITYAKKNRLHLSQKFHAIFQDRFTS
                     SKMATKYENVYNNLFREVL"
     gene            8885..9694
                     /gene="wclZ"
     CDS             8885..9694
                     /gene="wclZ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WclZ"
                     /protein_id="ACH97136.1"
                     /translation="MSFSVLMSLYNGESSEYLNDCLSSINSQILKPNQIVIVYDGPIR
                     KELDDIVTRWEKQLPIHCVKLTSCTGLGNALNYGLKFCDYDIVCRMDTDDICRNDRFL
                     RQVKILESDNNLALIGSYISEFDSTPSNSHAVRTVPIEHNDICLYAKKRNPFNHMTVA
                     FRKSVVTSLGGYKDEYLYEDYALWIKMLKNGFLTKNIPEALVYARVGNNMELKRGGWK
                     YFTSEVKAQYGFYKIGFLNKQEMIINIVLRAPVRLMPNFMRKIIYRNFLRN"
     gene            9708..10727
                     /gene="gne"
     CDS             9708..10727
                     /gene="gne"
                     /note="UDP-N-acetyl-glucosamine-4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gne"
                     /protein_id="ACH97137.1"
                     /translation="MSVLVTGGAGYIGSHTVLTLLQKGIDVIVIDDFSNSSLESLERV
                     KKITNRDIKVYKGDVADLRLLNLIFSNHDIHTVIHFAGSKSVGESISKPIHYYNNNVV
                     ASLVLINEMMKRGIYNLIFSSSATVYGNSNTMPVTENAPIGMTTNPYGTSKLMIEKIL
                     DDVTRANSSFRVTVLRYFNPVGAHFSGEIGEDPNGIPNNLMPYVCQVAIGKYKQVYYS
                     ISILYMYICTGVRDFIHVMDLAEGHVAALEHRNEGPNHKVYNLGTGKGYSVLDLLTTF
                     ERVTFRSVPYVLSERRPGDIAECWSDPSKAYRELGWKAKRGLEEMVRDAWNWQQKNPN
                     GYIKA"
ORIGIN      
        1 attggtagct gtaagccaag ggcggtagcg tgcattaata cctctattaa tcaaactaag
       61 agccgcttat ttcacagcat gctctgaagt aatatggaat aaaagagtga agatacttgt
      121 tactggtggc gcaggattta ttggttctgc tgtagttcgt cacattataa ataatacgca
      181 ggatagtgtt gttaatgtcg ataaattaac gtacgccgga aacctggaat cacttgctga
      241 tgtttctgat tctgaacgct atatttttga acatgcggat atttgcgatg ctgctgcaat
      301 ggcacggatt tttgctcagc atcagccgga tgcagtgatg cacctggcag ctgaaagcca
      361 tgttgaccgt tcaattacag gtcctgcggc atttattgaa accaatattg ttggtactta
      421 tgtccttttg gaagccgctc gcaattactg gtctgctctt gatagcgaca agaaaaatag
      481 cttccgtttt catcatattt ctactgacga agtctatggt gatttgcctc atccagatga
      541 agtaaataat aacgaagaat tacccttatt tactgagacg acagcttacg caccaagcag
      601 cccttattcc gcatcaaaag catccagcga tcatttagtc cgcgcgtgga aacgtaccta
      661 tggtttaccg accattgtga ctaactgttc gaataactac ggtccttatc actttccgga
      721 aaaattgatt ccactagtaa ttcttaatgc tctggaaggt aaggcattac ctatttatgg
      781 caaaggggat caaattcgtg actggctgta tgttgaagat catgcgcgtg cgttatatac
      841 cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacaca acgaaaagaa
      901 aaacatcgat gtagtgctca ctatttgtga tttgttggat gagattgtac cgaaagagaa
      961 atcttatcgt gagcaaatta cttatgttgc cgaccgcccg ggacatgatc gccgttatgc
     1021 gattgatgct gagaagatta gccgcgaatt gggttggaaa ccacaggaaa cgtttgagag
     1081 cgggattcgt aaaacagtgg aatggtacct gtccaataca aaatgggttg ataatgtgaa
     1141 aagtggtgcc tatcaatcgt ggattgaaca gaactatgag ggccgccagt aatgaatatc
     1201 ctccttttcg gcaaaacagg gcaggtaggt tgggaactac agcgtgctct ggcacctctg
     1261 ggtaatttga ttgctcttga tgttcactcc actgattact gtggtgattt tagtaatcct
     1321 gaaggtgtag ctgaaaccgt aagaagcatt cggcctgata ttattgtcaa cgcagcagct
     1381 cacaccgcag tagacaaagc agaatcagaa ccggagtttg cacaattact taacgcgacg
     1441 agtgtcgaag cgatcgcgaa agcagccaat gaagtcggcg catgggttat tcactactct
     1501 actgactacg tatttccggg gaccggtgaa ataccatggc aggaggcgga tgcaaccgca
     1561 ccgctaaatg tttacggtga aaccaagtta gctggagaaa aagcattaca agagcattgt
     1621 gcgaagcacc ttatttttcg taccagctgg gtatatgcag gtaaaggaaa caattttgcc
     1681 aagacaatgt tacgtctggc aaaagagcgc gaagaattag ctgtcattaa cgatcagttt
     1741 ggtgcaccaa caggtgctga actgctggct gattgtacgg cacatgcaat tcgtatggca
     1801 ctgaataaac cagaagtcgc aggcttgtac catctggtag ccagtggtac cacaacctgg
     1861 cacgattatg ctgcgctggt ttttgaagag gcacgaaaag caggtattcc ccttgcactc
     1921 aacaagctca aggcagtacc aacaacagcc tatcctacac cagctcgtcg tccacataac
     1981 tctcgcctta atacagaaaa atttcagcag aattttgcgc ttgtcttgcc tgactggcag
     2041 gttggtgtga aacgaatgct caacgaatta tttacgacta cagcaattta atagtttttg
     2101 catcttgttc gtgatggtgg agcaagatga attaaaagga atgatgaaat gaaaacgcgt
     2161 aaaggtatta ttttagcggg tggttctggt acacgtcttt atcctgtgac tatggctgtc
     2221 agtaaacagc tattacctat ttatgataag ccgatgatct attacccgct ctctacactg
     2281 atgttggcgg gtattcgcga tattctgatt attagtacgc cacaggatac tcctcgtttt
     2341 caacaactgc tgggtgacgg tagccagtgg gggctaaatc ttcagtacaa agtgcaaccg
     2401 actccagatg ggcttgcgca ggcgtttatt atcggtgaag agtttatcgg tggtgatgat
     2461 tgtgctttgg tacttggtga taatatcttc tacggtcacg acctgcctaa gttaatggat
     2521 gccgctgtta acaaagaaag tggtgcaacg gtatttgcct atcacgttaa tgatcctgaa
     2581 cgctacggtg tcgttgagtt tgataaaaac ggtacggcga tcagcctgga agaaaaaccg
     2641 ctacaaccaa aaagtaatta tgcggtaacc gggctttatt tctatgataa cgacgttgtc
     2701 gaaatggcga aaaatcttaa gccttctgcc cgcggtgaac tggaaattac cgatattaac
     2761 cgtatttata tggaacaggg gcgtttatcc gttgccatga tgggacgtgg ttatgcatgg
     2821 ctggacacgg ggacacatca aagtcttatt gaagcaagca acttcattgc aacaattgaa
     2881 gagcgtcaag gcttgaaagt ttcctgcccg gaagaaattg ctttcaggaa agggtttatt
     2941 gatgctgagc aggtgaaagt gttagccgaa ccgctgaaga aaaatgctta tggtcagtat
     3001 ctgctcaaaa tgattaaagg tgattaataa aatgaacgta attaaaacag aaattccaga
     3061 tgtactgatt tttgaaccaa aagtgtttgg ggatgaacgt ggttttttta tggaaagctt
     3121 taatcaacga caatttgaag aaattgttgg acgcaaaatt tcgtttgttc aggataatca
     3181 ttcaaagtca aaaaaaggtg ttttacgtgg ccttcattat cagttgcctc catttgcaca
     3241 aggtaaatta gttagatgta tcaatggtga agtatatgat gttgcagttg atctacgacc
     3301 tgaatcaatt aattatggta aatgggtagg catcattctt tctgctgaaa ataagaagca
     3361 gctatggata ccagaaggtt ttgcacatgg ttttttagtt ataagcgaat ttgctgaatt
     3421 tgcgtacaaa accacaaatt attattcaaa agaaagtgaa agaggcatac gttggaatga
     3481 tccaacgata aatatccaat ggccaaaaat gggacaatat ttactttctc cgaaagatgc
     3541 gttagcacca tttatagaat taagaattga ttaatggaaa acaagaaaaa tcatatttat
     3601 ggaattgtaa ataatattat tgtgatgtta tctcccatta taataattcc gtttgcaatt
     3661 aaaaatgtcg gcattgatga atatggctat tttgtacaag ttaatataat ttattcgtgt
     3721 ttgatatcca tattcactgc ttctctatcg ggatatttta taaagagcta tcttgagaaa
     3781 aaattatcct ttcatgatat ttttataatt caattgttta ttaatttgtt ctccactgcg
     3841 atcataggta tatatatact gctaagattt gatgtcgttg ctccgtatat atttgttgtt
     3901 gctatgctaa ctaatacttt aaatttcgaa tggttttttc atgcaattgg cgcacagaaa
     3961 caattattaa taaggaattt aattataaaa acaatgtttg taattatagc aatatttatc
     4021 ttagaagtat ataaaaatat tgctgtttat tttgttattt ttgggttgaa tattattatt
     4081 gcaaattttt tattgggtat atatagtttt acgaaaataa aaaataagga atatatactt
     4141 ttttccaatg ggaggggggg gaatcggttg ttttgtttta ttaaggatgc aaaattcttc
     4201 tttttgtcac cgacaattgg tgcagtatat caatatggcg atcaaatatt agtttcatta
     4261 ttattcccaa aaactgcttt ggtttttata aatttagcta aacaaataat tggtgcgtca
     4321 gtgatggtgt cgggtactct atgtagagtt gaacaaaaga acattttttc atctgagaaa
     4381 attgaaagat tgacaaaaat aaaaaaaaca cttatggcat atgtttttta tttgatgact
     4441 acatctatat taataatatt aattggtcct ttaattcttg gcatgttaat taaagaaact
     4501 gtatcacttg gattaaacta ttacattcta attgctggta tatatgtctt tacttcattg
     4561 agtatattta tcgattatat aataggattg actttcagga ctgagcatta cacttctatt
     4621 tcgaatattt gttgtgcaac catagttact ttattgaacg caatgttttt agaaaaatat
     4681 ggtgcaactt tttcgctttt ttcattattc attggagagc ttttggtttt tattttatta
     4741 tctacaatgc attatataaa tataaagcga aggacatatt ataatggtta atattctgtt
     4801 atgtattaat ataataatac tcttgatttg catttgtatc tatttgatcg ccttaaaaag
     4861 taaaaaagct ccacgtttat tcgcactata tttaggagct tttattcttt ttattgagtc
     4921 ccacataatt ctagcaataa caacttcttt taattttggt acgtcagaat ggttttttaa
     4981 tggagagttt gattataata caaaaacaga agttataaca agcatcaatc tatttataat
     5041 aggtatgata ttaggttcgg tatttattgc atcaacaata acctataaaa gttcttccta
     5101 tgatgtgact tttgagaaca aatcgatagc tagattttcg tggctactgt tagtatctat
     5161 cttaccattt gtagttgttt atttaattaa tttaatagct tttatttcat caaacggttt
     5221 ttattcttta tatattaacg gtaataaaat atcgggtgga tacattcttg atttgttttt
     5281 tctaacactt tactcattat tgatatcttt aaaaaataag aaaaagatac tgttcattat
     5341 cttgtgtgta gcctgtgttt atttattcat aggaacaaga ttggaattta tgtttaaagt
     5401 atttcctgtg ttaatctatt atatacttat ttcaaaaaat atacacaaat atttcaggtt
     5461 gaaaaatatc cttgctatat caatactctt ttggggttta atatttagta tgcaatactc
     5521 ggtttcagca agagataata ttgaaatggg gtcgaatatc atcacaacgt tcttaaaaca
     5581 acaaggcgta tcagtcaatg ttattggtat tgctataaag gacaaaaata attcattatt
     5641 aagtgaatct gtgattttgt cgccactata tgatagtgct atttctttag ctaattcttt
     5701 agtaggtgtc caaagcaatg gaaattcggt agagttcgca gagaattcat tctcattgtc
     5761 gcacaaattg agttatttag aagatccttc agcatacctt gctggctatg gtgttggggg
     5821 agccgctatt gctgagctgt acatagttgg tggttacttg gcttgtttaa ttggaggtat
     5881 gcttacatat atttttatct ctatactaga aaaaatcgcc aaaaaatcct tttttaattt
     5941 tatttttgtt atgttgataa ctgggaaaat attatactca cctcgagggg agtttctttc
     6001 atttatgtct gctgacagaa tgttaatatt atttttaatt tttacgtttt cttataaatt
     6061 tctattggca acttcaaata aaaaaatgag ttttaaaaat gaatgaactt gtatctataa
     6121 ttataccgtt atacaaccaa ggagagtata tcgaggaaac tctgaattcg gtcaagcaac
     6181 agtcttattc aaattatgaa ttgatcattg tagatgatgg cagtaacgat caattcacaa
     6241 ttgaaaagat aaaagaactc aggttcaaag gatatacgat tatctcaaag caaaacggcg
     6301 gattagcatc agcaagaaat gaaggtatta gaaatgctat aggcaaatat attattgttc
     6361 ttgattctga tgatataatt catcgcgatt atattaaaaa tcttcttgaa gttataacta
     6421 atacaaaagc acgaatcgta tatagtaagg ctttattgtt tgaatacaga acgggaatgt
     6481 ggatgctacc atcatttaca atgcgaagaa tgctgaaaag taatattatt ttttgttctg
     6541 ctatgtatta tcgtgaagat tggtttaatg tgggaggcta tgatgaatct ttaagaacag
     6601 gccttgagga ctgggatttt tggctatcat taataggttt atacaataat cataacgata
     6661 tcgttgcgag ggtaaataag cctctgtttt tttatcgaat tagaaggaat tcaatgctaa
     6721 gatcgataaa ttacactgaa aaagaaaaaa ttgttaatta tatacataaa aaacacattc
     6781 aattatttga actgtataaa gttaagcccc ttatttatag taaacagaat ttaatatcca
     6841 aaatttttaa taaaattgcc tccaaaatat gtggaatagg atacaatgtt aaacgataaa
     6901 aaggaaatgg ttgctattgt tcttgcctca tataatggac agttatatat taaagaacaa
     6961 gttttaagta ttttaaacca gagttatcaa tatattaaat tgtattgtag cgatgatggt
     7021 tctactgatg gaacaatcga ctatttaaag gagtatcaaa aaaataatat tgttattgca
     7081 aagaatgatg gaaaaaaagg ccctgcgtat aattttataa acggattaaa attggttggt
     7141 gatgaaaagt acatcatttt tagcgatcag gatgatgttt ggatgcctga aaaagtttca
     7201 acattgaagt cgtatgcaga taaattattg aataatgaca aaccgggggc aatttattgc
     7261 gacgctttaa ttgttgatca ttctcttact caacttggta aaagaatgta tggtgagcac
     7321 catatatgtc ctaccaaaat ctctgaactt ttatatttaa atggtggagt gcagggagca
     7381 tctatgctta ttaatcgaaa aatgatggat gaaatgttat catataataa atatatttat
     7441 atgcatgatc agttagcgac atatttagct gttgtttata agaatatcta ttatataaat
     7501 ataccattaa tgttgtacag gcaacattct aaaaatgtca ttggagttaa tactaataaa
     7561 atagagaaaa tgaaactgtt atttagttct aatatgattg atagtcgatc atataaattt
     7621 ataactgaat ttagtcaaca ctatgcttgt aacaatcata tcaatagtag aaatagtcta
     7681 gaatattata tcgagctgaa agaaaatata ttaaaatcta taataaagga atttttgaat
     7741 ggtaatgcta cacttcataa cagtcgaagt aaattactaa taaagctttt ctttaaattc
     7801 attattaata aagtcgttca aaaaaaatga aaatagcgta tgttgtgtct tctaagaaaa
     7861 aatgtggccc aaatattgtc atattgaaca ttgttaaaga attggctaat aaacatgaaa
     7921 tggaaatatt tttccttgat gagtctgatg atgatgtatt tgaatgtgtg aacgtaaagt
     7981 ctacacaaat aaaaaaagct tctgatttga aggagcatct aaaaaggttc gatataattc
     8041 attctagtgg aatacgtccc gatgcattgg ttgttttatg caaagttatt tatcgtgtga
     8101 aatgtaaaat aattacaaca atacataact atgtatttca agacctttat tactcatatg
     8161 gtttggttaa atccttaatt tggggcttac tttggtgttc tatctggcta tttttcgata
     8221 aattagtaat tctttcaaaa aatgccgata attattactg gtttctcccg tctgcaaaaa
     8281 aaaatatcat ttataatggg atagatgata atgattgcct tcaaaataaa aaatgtaatt
     8341 atcgtaagga atttaatatt cctgatgatg ggatattagc aggctcttgt gctaatttaa
     8401 ccaagtgtaa agggatcgat cttgttattc agaccttaac taaagaacat aaaatttact
     8461 atatagttgc cggtgatggt attgaaaaac ataatcttat taatcttgta aaagcgcgta
     8521 agctgcatga aagagtgtat tttatcgatt tccttgatga accagaaagt tttatgtccc
     8581 agttggatgt ttttttgatg ccatccagaa gtgaaggatt tggacttact gttttggaat
     8641 caactaagtt aggtatacct gtaataacgt ctaatattcc aatatttatg gagttatttg
     8701 atcaaatgtg tctcacattt gatattaaaa atccatctac attaatcgat gtaataacct
     8761 acgcaaaaaa aaatagattg catctttcgc aaaaattcca tgctatattt caagataggt
     8821 ttacttcttc aaagatggct acaaaatatg aaaacgtcta taataattta tttagagagg
     8881 ttttatgagc ttttccgttt taatgtcttt atataatggt gaaagttccg agtatcttaa
     8941 tgattgctta agtagcatta attcgcaaat attaaaaccg aaccaaattg taatcgtata
     9001 tgatggccca attagaaaag aattggacga tatagttaca agatgggaaa agcaattgcc
     9061 aatacattgt gtaaagctca cttcctgtac aggattaggt aatgcattga attatggttt
     9121 gaaattttgt gattacgata ttgtctgtcg tatggacact gatgatatat gtagaaatga
     9181 cagattttta agacaagtta aaatacttga aagtgataat aatctggctt tgattgggtc
     9241 ttatatctca gagtttgact ctactccctc aaacagtcat gctgttagga ccgttcccat
     9301 tgagcataat gatatttgtt tatatgcaaa aaaaagaaat ccgtttaacc atatgactgt
     9361 agcatttaga aaaagtgtag taacttctct cggtgggtat aaggatgaat atttatatga
     9421 agactacgct ctgtggatta agatgcttaa aaatggattt ttgactaaaa atattccaga
     9481 ggcgctagtt tatgcgcggg ttggtaataa tatggagctt aaacgcggtg ggtggaaata
     9541 ttttacatca gaagttaagg ctcaatatgg cttctataaa attggttttc ttaacaaaca
     9601 ggaaatgatt attaatattg ttttgagagc accggttcgt ttaatgccta actttatgag
     9661 aaagattatt tatagaaatt tccttagaaa ctgatatagg tatataaatg agtgtattag
     9721 tgacaggtgg ggcaggttat attggtagtc atacagtttt gactctctta caaaaaggaa
     9781 tagatgtcat tgtgattgat gatttttcta attctagctt agagtctttg gaaagagtta
     9841 aaaaaattac aaatcgagat attaaagtat ataaggggga tgtagcagat cttcgtttat
     9901 tgaatcttat tttttctaat catgatattc atacggttat tcattttgct ggttctaaat
     9961 ctgttgggga gtctatatca aaaccaattc attactataa taacaatgtt gtagctagcc
    10021 ttgttcttat taatgaaatg atgaaaagag gtatttataa tttaattttt agttcatctg
    10081 caacagtata cggtaactcg aataccatgc cagttactga aaatgcacct attgggatga
    10141 ctacaaatcc ttatggcact tctaaactta tgattgaaaa aatattagat gatgttacaa
    10201 gggcaaattc gagctttaga gtgacggttt tgaggtattt taaccctgtg ggtgctcatt
    10261 tctctggaga gattggtgag gatccaaatg gtattccaaa taatcttatg ccatatgttt
    10321 gtcaggtggc aattggtaaa tataaacaag tatactatag tataagtata ctatacatgt
    10381 atatatgcac tggtgtccgt gattttatcc atgtaatgga tctcgctgaa ggtcatgttg
    10441 ctgcgttgga acatagaaac gaaggtccca atcataaagt ttacaacctc ggaacaggaa
    10501 aggggtattc tgttttggat cttttgacca cttttgaacg agtaaccttt cgatccgtac
    10561 cttacgtttt gagtgaaaga cgccctggag atatcgccga atgttggtct gatccttcta
    10621 aggcttatag ggaacttgga tggaaagcga agcgtgggtt ggaagaaatg gttcgagatg
    10681 cctggaattg gcaacaaaag aatccgaacg gttatataaa agcatgaatg ctctatggta
    10741 tttttgtcaa cggtaaaaat tatcgatttt atgtatcctg agttaatata gcatctacat
    10801 tgacctgagt tatgtttcgc agtatcaccc tgacaggagt aaacaatgtc caagcaacag
    10861 atcggcgtcg tcggtatggc agtg
//

</pre>