<pre>LOCUS       AF361371               12105 bp    DNA     linear   BCT 23-JUL-2016
DEFINITION  Escherichia coli O104 antigen gene cluster, complete sequence.
ACCESSION   AF361371
VERSION     AF361371.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12105)
  AUTHORS   Wang,L., Briggs,C.E., Rothemund,D., Fratamico,P., Luchansky,J.B.
            and Reeves,P.R.
  TITLE     Sequence of the E. coli O104 antigen gene cluster and
            identification of O104 specific genes
  JOURNAL   Gene 270 (1-2), 231-236 (2001)
   PUBMED   11404020
REFERENCE   2  (bases 1 to 12105)
  AUTHORS   Wang,L., Briggs,C.E., Rothemund,D., Fratamico,P., Luchansky,J.B.
            and Reeves,P.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAR-2001) Department of Microbiology, The University
            of Sydney, Sydney, NSW 2006, Australia
FEATURES             Location/Qualifiers
     source          1..12105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O104"
     gene            141..737
                     /gene="galF"
     CDS             141..737
                     /gene="galF"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate uridylytransferase"
                     /protein_id="AAK64366.1"
                     /translation="MNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPL
                     RYNLAAMIARFNETGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQ
                     PQTLDSDIMAVGRYVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGD
                     SYDCGKKMGYMQAFVKYGLRNLKEGAKFRKGIEKLLSE"
     gene            1098..1718
                     /gene="wckD"
     CDS             1098..1718
                     /gene="wckD"
                     /codon_start=1
                     /transl_table=11
                     /product="WckD"
                     /protein_id="AAK64367.1"
                     /translation="MKKKLIIIGAGGFAKAVIDSLDHNEYALEGFVDSLKSGMHQGYP
                     IVGHSLSDISLPTEYYYFIAIGDPDDRALWLSQIRDLKLETINVIDKTAIVSTRSSIG
                     TCIYIGKMAIVNCDSQLEDGVVINTRALIEHGNHISYCTNISTNVVLNGDVYVGEKSF
                     IGSCTVVNGQIKIGSSTIVGSGSVVIRNIPNNVVVAGTPTRLIRER"
     gene            1723..2763
                     /gene="nnaB"
     CDS             1723..2763
                     /gene="nnaB"
                     /note="NeuNAc condensing enzyme"
                     /codon_start=1
                     /transl_table=11
                     /product="NnaB"
                     /protein_id="AAK64368.1"
                     /translation="MSKIYIVAEIGCNHNGDFNLAKKMVEEAKKAGVDAVKFQTFKAD
                     QLISKYAPKAEYQIKVTGNQESQLEMTRKLELPYEEFIKLEEYAKELGLDVFSTPFDF
                     ESIDFLASRNQKMWKIPSGELLNLPYLEKISRLPIEGKEIVLSTGMATIEEIKASVNV
                     LTKNGMAPEKITILHCNTEYPTPYEDVNLNSIIGLKNEFKGYNVGFSDHSPGYYAAIA
                     SVPYGITFIEKHFTLNKNFEGPDHKASVTPDELRLLCEGIRAVEKCLGSYAKIVTKSE
                     QKNKIVARKSIVAKCNIKKGEIYTIDNITTKRPGNGISPMYWYDILGKKAEKNFDEDQ
                     LISHSGFDEQEI"
     gene            2769..4034
                     /gene="nnaC"
     CDS             2769..4034
                     /gene="nnaC"
                     /note="CMP-NeuNAc synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="NnaC"
                     /protein_id="AAK64369.1"
                     /translation="MTNRIAIIPARAGSKGLPNKNVLMLIDKPLIAYTIEAAKESQCF
                     NKIIVSTDSLEYKSIAERYGAEVIIRSEELASDTATTYMVIENVLSHNKDCDYFMLLQ
                     PTSPFRNAHHIKEAITLFEGDVEANFLVSMTESEISSDLMKTLDSSMRLSNFNNDYSG
                     YRRQDNQKEYSPNGAIFIGTVEEYLKKKHFFGADSLAYIMNKADSIDIDDRLDFEFAI
                     SRMLQKNKNKIDKEVVARRIKQKFSITHAIKPITLIGHSIFDNWELSDLAGKEVNNYG
                     VSGITSEDYYNLILDANLIKEIGDVVFLMLGTNDIVIQGWDENDTLRWLMKIIDRIVK
                     LNPNVIIYFLSLPPVNGRVDRNNKVIEHLNAFLFKGLDKINHVRWVSLSDMFYDDFGN
                     LSADFTYDGLHFNGKAYRQLKKELMELIK"
     gene            4031..5194
                     /gene="nnaA"
     CDS             4031..5194
                     /gene="nnaA"
                     /note="GlcNAc-2-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="NnaA"
                     /protein_id="AAK64370.1"
                     /translation="MKKILYVTGSRAEYGIMKRLLQQLKEDDSIDLSIVATGMHCDKK
                     YGMTYKVIENDGFRVDKIIDIEINNDTNAEIIKTMSRCLSEFGIYFQDNKFDAVMILG
                     DRYEIMSVALAAAMHNLPIIHFHGGEKTLGNYDEFIRHSITKMSSLHLASTHEYRKRI
                     IQMGEHPSNVYDIGALGAENSLLLTLPPKDVLEQKFGGLSKPYFMVVFHPETLSGVSP
                     EIQVQSLLKSLHTFIKKYNFVFIGSNSDTGSDKITQKIKDFCKENNTKYFVSILPEEY
                     LALCKYSQGLIGNSSSGLIEVPSLGVPTVNIGDRQKGRVHGATVINCECSTEGISEAI
                     KKCLDEHYINGIKNAVNPYYKHNVMNNAYKHIKEFLLSNHEQYKDFYDLSFEL"
     gene            5279..6229
                     /gene="wbwA"
     CDS             5279..6229
                     /gene="wbwA"
                     /note="putative glycosyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbwA"
                     /protein_id="AAK64371.1"
                     /translation="MKMRNNVFVFDSPYCLLIYCILFNHQLHDTIYIYSDNVSLRDIN
                     FPGKERYIIKKGNNKTSKIFYYFCFLFRLIINPKLRKLISNRKDYRFLGQDHLFFSKP
                     FLNEFILLEDGLANYRYPHYSRLYKLIIGGHTFGRSTKVNKILLSGMIDIIDPTINDK
                     VEFFDLIAAWEKLNSVQKKEINHIFNYCIEDELVADVMILTQPLSEDGFISENEKIRL
                     YDEIIKEYDDKKIVIRQHPRELTDYSLYFKDVKVNKVKAPVELVVLNSPMIKTAVTLF
                     SGGIFNIPCREKIFKGTSFSPLLSKHFGNISNKFEVKGKK"
     gene            6226..7338
                     /gene="wzy"
     CDS             6226..7338
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="AAK64372.1"
                     /translation="MTSYFIYNFYVLFAIIFAYVHERINLFTSRGVKFLSIGCVFCSL
                     FLLSALRFDVGPDYETYVYIYKNIVAGRIDITYDKYFFGYLSTLFSSLDRGYVLVFAV
                     YSFLTLFFVFKVILERKIFLWGIIVFCFVGFYLDSLDRIRQVLAVAIFLYSIKFIEKK
                     EPLRYFIAIIIASFFHLSALMLMPVYFYNRFKFEPLTLIIGLFGLLVLYLSGFTTEIQ
                     ALIYNNIPYYNEIYGNSQFAHLQGGFGTGLGFVFHIFLIMFSIYNLKDNYILRNLLFT
                     AGLLMIIAPGNLNIMRFGQYFYISLIIALPLAIKSLKKEYSYNLLLKCLFFCLLFIFF
                     QSQQSRNNYSYQSIFSDSFNSELFTPRSSDRTIINH"
     gene            7344..8567
                     /gene="wbwB"
     CDS             7344..8567
                     /gene="wbwB"
                     /note="putative glycosyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbwB"
                     /protein_id="AAK64373.1"
                     /translation="MKNILFINNALSGTGGARVILNLAKSLRNRGHKVALLLDRVDNI
                     HYKIDDGIEIYVRDKFKIKKVNPSTINKNDSQAFFTNQSNSVFFNFIKLYAKKLKSFF
                     YFLKAPCEMYAFKKFINTKKFDAIINNNVYVNVDRIYFESKLSNNYYVNFHNSPIEIF
                     SRREFSTLLPLSKIFRNVKLLSVSKGIADELLTLKGFNDKEIITIYNPFNFTELEGNS
                     RIQCEGNIPLPKEFIVTVSTLTDRKRVDRTIKAMPKIIREYGEIDLLIIGEGQLRNDL
                     QNLVKELNIEKYVHFLGFQTNPYYFINKAQLLILSSDSEGLPTVIIESLILGTPVLST
                     DCPTGPNEILVNWGDESLVSLTKTSDENNICNELAEKAIRLLCKKYDKKYVKHKSDLQ
                     RFDEKKISTSWEILF"
     gene            8549..9844
                     /gene="wzx"
     CDS             8549..9844
                     /gene="wzx"
                     /note="O antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="AAK64374.1"
                     /translation="MGDIILMNMLKYIQPILSLLLRMVTVISSFLFSWIVARLFGAES
                     AGTIFFYITIITIFVTIASQGAELGIAKAVARLAGNEKKQIGVLRSVVLRTNKMFLIL
                     SPAFLFYLLYEGYEINNLIILFVLMLILGYCFLYMNILSFFYQGIGNITMMILSQRAI
                     FNLFSFLLIAFLWGVFYLENKKLSFMTLEQQFVIITIAGLISFGLLYLYHRLTSNYSP
                     LYYKPEGFDKSCKELFKIQLLQIITMYGSQIIINIFSVKADIAGFIVAQRISTLLGFF
                     VLAISGVISSQVSRAYSLKDFTSIHKSAYHSVLFSSFLGVPVAGILILYSKECLLLFG
                     NDFYQYQAVLIVLIVSQVFNCLTGACDIILMFIDGEKEHKKNVLTGTFLAIVLSFILI
                     PIYSALGAAIATAFSAIIVNILDVISIKKKAGFWIFKTV"
     gene            9869..10675
                     /gene="wbwC"
     CDS             9869..10675
                     /gene="wbwC"
                     /note="putative glycosyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbwC"
                     /protein_id="AAK64375.1"
                     /translation="MKFSVLLSLYYKESALFLHDCLESIASNTCAPDQIVIVFDGYIS
                     DDLLHVVNEFSLRLPIDIVRLSNNVGLGKALNHGLQYCRNELIFRMDTDDICLPERFA
                     LQLSYMKSHPEVVLLGSAIEEFDNTMKIRQGKRFSVIYHEDIKKFAKKRNPFNHMTVV
                     FRKSVIEKLGGYQHHYLMEDYNLWLRILAADYRTHNLSEVLVNVRAGRNMLCRRKGYS
                     YIKSEILLAKLKYELQLDNLTGVIYTGVIRIIPRILPVSLLKLVYNILRK"
     gene            10796..>12105
                     /gene="gnd"
     CDS             10796..>12105
                     /gene="gnd"
                     /note="6-phosphogluconate dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gnd"
                     /protein_id="AAK64376.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAEDPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLP"
ORIGIN      
        1 tcctggtaac tcacgcgtcc aagaacgcag tcgaaaacca cttcgacacc tcttatgaat
       61 tagaatctct ccttgagcag cgcgtgaagc gtcaactact ggcggaagtt cagtccatct
      121 gcccgccggg cgtgaccatt atgaacgtgc gtcagggcga acctttaggt ttgggccact
      181 ccattttatg tgcgcgacct gccattggtg acaacccatt tgtcgtggtg ctgccggacg
      241 ttgttatcga tgatgccagc gccgacccgt tgcgttacaa ccttgctgcc atgattgcgc
      301 gcttcaatga aacgggacgc agccaggtgc tggcaaaacg tatgccgggt gacctctctg
      361 aatactccgt catccagacc aaagaaccgc tggaccgtga aggcaaagtc agccgcattg
      421 ttgaattcat cgaaaaaccg gatcagccac aaacgctgga ctcagacatc atggccgtgg
      481 gtcgctatgt gctttctgcc gatatttggc cggaacttga acgcactcaa cctggtgcat
      541 gggggcgtat tcagctgact gatgccattg ctgaactggc gaaaaaacag tccgttgatg
      601 caatgctgat gactggtgac agctacgact gcggtaaaaa aatgggctat atgcaggcgt
      661 ttgtgaagta tggcttacgc aacctgaaag aaggggcgaa gttccgtaaa ggtattgaga
      721 aactgttaag cgaataatga aaatctgact ggatgtaacg gttgataaga aaattataac
      781 ggcggtgaag attcgtggcg aaagtaattt gttgcgaata ttcctgccgt tgttttatat
      841 aaaccatcag aataacaacg agttagcaat aggattttag tcaaagtttt ccaggatttt
      901 ccttgtttcc agagcggatt ggtaagacaa ttagaatatc ttcttttaaa gttttaaaat
      961 aatttaaaag gattctaatt tcaaaaaata aatctctaca gtgcactggt agctgttaag
     1021 cctggggcgg tagcgtgtct gataatgccc tcactggaag gcgaattcaa atgctaaact
     1081 tcacttatgg tgcgtgaatg aagaaaaaat tgatcataat cggggctggt gggtttgcta
     1141 aagcagtaat agatagtctg gatcataatg aatacgctct tgaagggttt gttgattcac
     1201 tgaaatccgg aatgcatcaa ggatatccta tcgttggtca ttcattaagt gatattagtt
     1261 tgccaactga gtactattat tttattgcca ttggtgatcc tgatgataga gcattatggt
     1321 taagtcaaat acgagatcta aagcttgaaa cgataaatgt cattgataaa acagcaattg
     1381 tatcaacaag atcttcaatc ggaacatgta tttacattgg gaaaatggct atagtaaatt
     1441 gtgattcaca attagaggat ggagtcgtaa ttaatactag agccttgata gaacatggga
     1501 atcatatttc atattgtacg aatatttcaa ctaatgtggt ccttaatggt gatgtatatg
     1561 ttggagagaa aagttttata ggtagttgta ctgttgtaaa tgggcaaata aaaataggaa
     1621 gctcgacaat tgttggatct ggatcagtag taatacgaaa catccctaac aatgttgttg
     1681 ttgcaggaac accaacaaga ttaataagag aaaggtaagc aaatgagtaa aatatatatt
     1741 gtggctgaaa ttggatgtaa tcataatggt gattttaatc ttgcaaaaaa aatggttgaa
     1801 gaagccaaaa aagctggtgt tgatgcagtc aaatttcaga cttttaaagc tgatcaatta
     1861 atttcgaaat acgcacctaa ggcagagtat cagataaaag taactggtaa tcaggaatcc
     1921 caattagaaa tgacaaggaa gcttgagctg ccttatgaag agtttataaa gcttgaagaa
     1981 tatgcaaaag agttaggact tgatgtcttt tcaacaccat ttgattttga atctatcgat
     2041 tttcttgctt caagaaatca aaaaatgtgg aaaatacctt caggtgaatt gcttaatctc
     2101 ccgtatcttg aaaaaataag cagattgccg attgagggaa aagagattgt attgtcaaca
     2161 ggaatggcca caattgaaga aatcaaagca agtgtaaatg ttcttactaa aaatggaatg
     2221 gctcctgaaa agatcacaat attacattgt aacactgaat atcctacacc atatgaagat
     2281 gtaaatttaa actcaattat tggtctaaag aatgaattta aaggatataa cgttggcttt
     2341 tctgaccatt caccaggtta ttatgcagct attgcatcag ttccgtatgg aattacattc
     2401 atcgaaaaac attttactct aaataaaaat tttgaagggc ctgatcataa ggcatcagtt
     2461 acaccagatg agctacgatt attgtgtgag gggattcggg ctgttgaaaa atgtttagga
     2521 agctatgcga aaatagtaac aaaatctgaa caaaaaaata agatagtagc aagaaagtcc
     2581 atagtggcaa aatgcaacat taaaaaaggt gaaatatata cgattgataa tatcaccaca
     2641 aagcggccag gaaacggaat tagtcctatg tattggtatg atatcttagg caagaaggcg
     2701 gaaaaaaatt tcgatgagga tcaattaata agtcattctg gattcgatga acaggagatc
     2761 taatttaaat gactaatagg atagcgataa ttccggctag agcgggatca aaagggttac
     2821 caaataaaaa tgttttaatg ttaatagata aacctcttat agcttatact atagaggcgg
     2881 caaaagaatc gcagtgcttt aataaaatta ttgtttcaac agattcatta gaatacaaaa
     2941 gtatcgctga gagatacggt gcagaagtta ttattagaag tgaagagctt gcatcagata
     3001 ctgcaactac atatatggtt atagagaatg tattgagtca caataaagat tgtgactatt
     3061 ttatgttatt acaaccgacg tctcctttta gaaatgccca tcatataaag gaagccatta
     3121 cgctttttga gggggatgtt gaagccaatt ttttagtttc aatgacagaa agcgaaatat
     3181 cctcagattt aatgaaaact ttagattcct caatgcgact gagtaatttc aataatgact
     3241 acagtggcta tagacgacaa gataatcaaa aagaatacag tcccaatggc gcaattttta
     3301 ttggcacagt tgaagagtat cttaaaaaga aacatttttt tggcgctgat agcttggctt
     3361 atattatgaa taaggctgat tcaatagata ttgatgatag gctggatttt gaatttgcta
     3421 tttcacggat gttacaaaaa aataaaaata aaatagacaa agaagttgtt gcgcgacgaa
     3481 ttaaacaaaa attttcgata actcatgcca taaaaccgat cacattaata ggtcactcca
     3541 tatttgataa ctgggagtta tctgatttag caggtaagga ggtaaataat tatggtgtct
     3601 ctggtattac atctgaagat tattataacc ttatccttga tgctaatctc ataaaagaaa
     3661 ttggtgatgt ggttttttta atgttgggta caaatgatat tgttattcag ggttgggatg
     3721 agaatgacac cttaagatgg ctcatgaaaa taattgatag aatagtaaaa cttaatccaa
     3781 atgtaattat ctacttttta tcattgcctc cagttaatgg aagggttgat agaaataata
     3841 aagtcattga gcatttgaat gctttcttgt ttaagggttt agataaaatt aatcacgtca
     3901 gatgggtatc actttctgat atgttttatg atgactttgg gaatttatct gctgatttta
     3961 catatgatgg gttacatttc aacggaaaag catatagaca actaaaaaaa gaacttatgg
     4021 agttaataaa atgaaaaaga ttttatatgt aacaggttct cgtgctgaat atggaataat
     4081 gaaaagatta ttacagcaac taaaagaaga tgatagtata gatctatcta tagttgcaac
     4141 tggaatgcac tgtgataaga agtatggaat gacatacaaa gttattgaaa atgatggttt
     4201 tcgtgtcgat aaaataatag atatcgagat taataatgac acgaacgctg agataataaa
     4261 aaccatgtcg agatgtttat ctgaatttgg tatatatttt caagataata agtttgatgc
     4321 tgtgatgata cttggggata ggtatgaaat catgtcggta gcattagcag cagctatgca
     4381 taacttacca attattcatt ttcatggagg tgagaaaact ttaggtaatt atgacgaatt
     4441 tattaggcat tcaatcacta aaatgagttc attacatctg gcctcaacgc atgaatacag
     4501 aaaaaggata attcagatgg gggagcatcc atctaacgtt tatgatatag gtgctcttgg
     4561 agctgaaaat tcacttttac ttactctacc accaaaagat gttcttgaac aaaaatttgg
     4621 agggttatct aaaccttatt ttatggttgt ttttcatcct gaaacgctta gcggtgtttc
     4681 tcctgaaatt caagttcaat ctttattaaa aagtttacac accttcatta aaaaatataa
     4741 ttttgttttc attggttcta attctgacac tggttcagac aaaatcacac aaaaaattaa
     4801 agatttttgt aaagagaata atacgaaata ttttgtttct atactaccag aggaatatct
     4861 tgcactatgt aagtactcgc aaggtttaat aggtaattct tcttctggtt taattgaagt
     4921 tccttcgctt ggagtaccca cagttaatat tggagacagg caaaaaggga gggttcacgg
     4981 agctactgtt ataaattgtg aatgcagtac tgaaggcatt tctgaagcaa taaaaaaatg
     5041 cttagatgaa cattacatta atggtattaa gaatgcggtg aacccttatt ataaacataa
     5101 tgtgatgaat aatgcctata agcatatcaa agaattcctg ttaagtaatc atgaacaata
     5161 taaggatttt tacgatttgt catttgaact ttaaggtaac agccctaagt ttatttaagc
     5221 cttactgtaa actaggtgtt taaaaataga caaacctttc gtagtataga gctatataat
     5281 gaaaatgaga aataatgtat ttgtttttga ttctccatat tgcttgctta tttactgtat
     5341 attatttaac catcaattgc atgatactat atatatatat tcagataatg tttcgcttcg
     5401 tgatataaat ttcccaggta aagagcgata tataattaaa aaagggaaca acaaaactag
     5461 taaaatattt tattattttt gttttttatt caggttgata ataaatccga agttaagaaa
     5521 attaatttct aatagaaaag actatcgatt tttagggcaa gatcatcttt ttttttctaa
     5581 acccttctta aacgagttca tcttgcttga ggacggcttg gcaaattata gatacccaca
     5641 ttattctcgt ctatataaac ttataatagg aggccataca tttgggcgct ctaccaaagt
     5701 aaataaaata cttctttctg gaatgattga tataattgat ccaactataa atgacaaagt
     5761 tgagtttttt gaccttattg cagcttggga aaaacttaat tcagtacaaa aaaaagaaat
     5821 taatcatatc tttaattact gtatcgaaga tgaattggtt gctgacgtca tgattttaac
     5881 tcagccttta agtgaagatg gttttattag tgaaaatgaa aaaatcagat tatacgatga
     5941 aataataaaa gagtatgatg ataaaaaaat agtaataaga cagcatcctc gcgaattgac
     6001 ggattattca ttatatttta aagatgtgaa agtgaacaag gttaaggctc ctgttgaatt
     6061 agtagtgctt aattctccaa tgattaaaac agctgttact ctattctctg ggggaatatt
     6121 taacattcca tgtcgcgaaa aaatattcaa aggaacatca tttagtcctt tattatcaaa
     6181 acattttggt aatatatcaa ataaatttga agttaaaggt aagaaatgac atcttatttc
     6241 atatataatt tttatgtatt atttgcaatt atctttgctt acgttcatga aagaattaac
     6301 ttatttactt cacgaggtgt caagttttta agcataggat gtgtattttg ttccttgttt
     6361 ttattatctg cgctacgttt tgatgtaggc cctgactatg aaacgtatgt atatatatat
     6421 aaaaatatag tagcaggtag aatagatatt acatatgata aatacttttt tggatatctg
     6481 tctacattat ttagttcatt agatcgaggt tatgttcttg tctttgcagt ctactcattt
     6541 ttaaccttgt tttttgtgtt caaagttatt ttggagagaa agatattctt atggggaata
     6601 atagtttttt gctttgttgg tttttattta gattcacttg accgtatcag acaagtatta
     6661 gccgttgcca tatttttgta ttcaataaaa tttatagaaa aaaaagagcc tttaagatat
     6721 tttatagcca ttatcattgc tagttttttc catttatctg cattaatgtt aatgcctgtt
     6781 tatttttata atcgatttaa atttgaacca ttaactttaa taatcggttt atttggtttg
     6841 ttagtgttgt atctttcagg ttttacaacg gaaatacaag ccttaatata taataatata
     6901 ccatattata atgaaatata tggcaatagc caatttgcac atttgcaagg agggttcggc
     6961 actggacttg ggtttgtttt tcacatcttt ctaataatgt tttcaattta taatcttaaa
     7021 gataattata tattaagaaa tttattgttt actgctgggt tattaatgat aattgccccg
     7081 ggtaatttaa atataatgag gtttgggcaa tatttttata tatcattgat tattgcttta
     7141 ccattagcca tcaaaagcct taaaaaggaa tacagttata atcttctttt gaagtgttta
     7201 tttttttgtt tgctttttat tttttttcaa tcgcaacaaa gtagaaataa ctactcatat
     7261 caatctatct tttcagatag ctttaactcc gaattattta ctccacgatc atcggataga
     7321 acaattatca atcactaatc tatatgaaaa atatactatt tattaataac gctttaagcg
     7381 gaacaggtgg ggctagagtt attcttaatt tagctaagtc tcttcggaat agaggtcata
     7441 aagtagcatt acttcttgat cgcgttgata atatacatta taaaatagat gatgggatag
     7501 agatttatgt tagagataag tttaaaataa aaaaagtgaa tccttcaact ataaataaaa
     7561 atgattcgca agctttcttt actaatcaaa gtaattctgt tttttttaat tttataaaac
     7621 tatatgctaa gaaattaaag agtttttttt attttttaaa agctccatgc gagatgtatg
     7681 catttaagaa atttattaac actaaaaaat ttgatgcaat tattaataat aatgtatatg
     7741 tcaatgttga taggatttat tttgagagta aattatctaa taactattac gtcaactttc
     7801 ataacagtcc aattgagata ttcagtagaa gagaatttag tactttactt ccattgagca
     7861 aaatatttcg taatgttaaa ttactttctg tatcaaaagg aattgcagat gagttactga
     7921 ccttaaaagg tttcaatgat aaagagatta taactatata taatcctttt aattttactg
     7981 aactagaagg taattcaaga atccaatgtg aaggaaatat acccttacca aaggaattta
     8041 ttgtgactgt ctctacatta acggatagaa agagagttga tagaacaata aaagctatgc
     8101 ccaaaattat aagagagtat ggagaaatag atttacttat tattggcgaa ggacaacttc
     8161 gcaatgattt gcaaaattta gtcaaggaac ttaatattga aaaatatgtt catttcttag
     8221 gttttcagac taatccatac tattttatta ataaagctca gcttctaatt ttgagttctg
     8281 attcagaagg attacctacc gtaattatag agtcactaat attaggcacg ccggtattaa
     8341 gtactgattg tcctactgga ccaaatgaaa ttcttgtaaa ctggggtgac gaatcattag
     8401 tttctcttac aaaaacctct gatgagaaca atatatgcaa tgagcttgcc gaaaaagcca
     8461 tcagactttt atgtaagaaa tatgataaga aatatgtaaa acataaatca gacctgcaaa
     8521 gattcgacga aaaaaaaatc tccacatcat gggagatatt attttaatga atatgttaaa
     8581 atatatacag ccaatattat cacttttatt aagaatggtt actgttattt cttcgttcct
     8641 ttttagctgg attgtggctc gactttttgg tgcggaaagt gctggtacta ttttcttcta
     8701 tataacaatt attacaattt ttgtgaccat tgcttctcaa ggtgctgagc ttgggatcgc
     8761 gaaggcagta gcacgtttag ccggaaatga gaaaaaacaa atcggtgtat taagaagtgt
     8821 tgtcttaaga acaaataaaa tgtttttaat tttgtcacca gcatttttat tttatttgtt
     8881 atatgaaggc tatgaaataa acaatttaat tattttgttt gttttaatgt tgatactagg
     8941 atattgtttt ctttatatga atattctttc atttttctat caaggaatag gaaatataac
     9001 gatgatgatc cttagtcaac gggctatttt taatctattc tcgtttttat tgattgcgtt
     9061 tctatgggga gtattttatt tagaaaataa aaaattatca ttcatgacgc tagaacaaca
     9121 gttcgtgatt attactattg cgggattaat atcctttgga ctgttgtatt tatatcatag
     9181 attgacatcg aattattcac ctttatatta taaaccagaa gggtttgata aatcctgtaa
     9241 agagctattt aaaattcagc ttctgcaaat aattacaatg tatggctcac aaattattat
     9301 taatattttt tcggtcaaag cagatatcgc aggttttatt gtcgcgcaaa gaatttcaac
     9361 tttacttggt ttttttgtat tagcaataag tggtgtcatt tcaagtcagg tttctagggc
     9421 gtatagttta aaggatttta catctattca taaaagtgcg tatcattcag ttttattttc
     9481 ttcatttttg ggagtgcctg tagcaggaat tttgattcta tattctaaag aatgtttatt
     9541 actatttgga aatgatttct atcaatatca ggcagttttg atagtattaa tagtatctca
     9601 agtatttaat tgcttaactg gtgcatgtga tataattctc atgtttatcg atggggaaaa
     9661 agagcataag aaaaatgtcc ttacgggaac ctttcttgca atagttcttt cctttatatt
     9721 gattcctata tattcagctt taggcgcagc tattgcaact gctttttcag caattatagt
     9781 taacattctt gatgtgatat ctattaagaa aaaagctggt ttttggattt ttaaaactgt
     9841 ataaaacatt cagggtttgt atatcataat gaaatttagc gttctgttat ccctgtatta
     9901 caaagagtct gcactttttc tgcatgattg tttagaaagt atagcaagca atacatgcgc
     9961 acctgatcaa attgtaattg tctttgatgg ttatattagt gatgatttgc tacatgttgt
    10021 taatgaattt tcattacgtt tacccattga tattgtgcga ttaagtaata atgttggtct
    10081 aggtaaggct cttaatcatg gtctacagta ttgtcgcaat gaactgattt ttaggatgga
    10141 tactgatgat atttgtttgc ctgaacggtt cgctcttcaa cttagctata tgaaatcaca
    10201 ccctgaagtt gttcttttgg gaagtgccat tgaagaattt gataatacaa tgaaaattag
    10261 gcaaggaaaa agattttcag ttatatacca tgaagatata aaaaaatttg caaaaaaaag
    10321 aaatcctttc aaccatatga ctgttgtttt cagaaagtcc gtaattgaaa agcttggtgg
    10381 atatcaacat cattatctaa tggaagatta taacttatgg cttaggatac ttgcagccga
    10441 ttatcgcact cataatttga gtgaggttct tgttaatgtt cgggcaggca gaaatatgct
    10501 gtgtcgacgg aaaggttatt cctatattaa gagtgagata cttttggcga aattaaaata
    10561 tgaattacag ttggataatc tgacaggtgt catatataca ggtgtaattc gtattatccc
    10621 tagaatattg cctgtttcat tactgaaatt agtatacaat atattgcgga aataatttcc
    10681 tccgctcatt tactattgta atatcacttt tattaagctt ttcaaatcaa cttagttacg
    10741 ttagtattta atatacttta cccgctccgg cggtgccccc tgacaggagt aaacaatgtc
    10801 aaagcaacag atcggcgtag tcggtatggc agtgatgggg cgcaaccttg cgctcaacat
    10861 cgaaagccgt ggttataccg tctctatttt caaccgttcc cgtgaaaaga cggaagaagt
    10921 gattgccgaa aatccaggca agaaactggt tccttactat acggtgaaag agtttgttga
    10981 atctctggaa acgcctcgtc gcatcctgtt aatggtgaaa gcaggtgcag gcacggatgc
    11041 tgctattgat tccctcaaac catatctcga taaaggcgac atcatcatcg atggtggtaa
    11101 caccttcttc caggacacca ttcgtcgtaa ccgtgagctt tctgccgaag gctttaactt
    11161 tatcggtacc ggtgtttctg gtggtgaaga aggtgcgctg aaagggcctt ccattatgcc
    11221 tggtgggcag aaagaagcct atgaactggt tgcgccgatc ctgaccaaaa tcgctgcagt
    11281 ggctgaagac ggtgagccat gcgttaccta tattggtgct gatggtgcag gtcactatgt
    11341 gaagatggtt cacaacggta ttgaatacgg tgatatgcag ctgattgctg aagcctattc
    11401 tctgcttaaa ggtggcctga acctttccaa cgaagaactg gcgcagacct ttaccgagtg
    11461 gaataacggt gaactgagca gctacctgat tgacatcacc aaagatatct tcaccaaaaa
    11521 agatgaagac ggtaactacc tggttgatgt gattctggat gaagcagcaa acaaaggtac
    11581 cggtaaatgg accagccaga gcgcgctgga tctcggcgaa ccactgtcgc tgattaccga
    11641 gtctgtgttt gcacgttata tctcttctct gaaagatcag cgcgttgccg catctaaagt
    11701 tctctctggt ccgcaagctc agtcagcagg cgacaaggct gagttcatcg aaaaagttcg
    11761 ccgtgcgctg tatctgggca aaatcgtttc ttacgctcag ggcttctctc agctacgcgc
    11821 cgcgtctgaa gagtacaact gggatctgaa ctacggcgaa atcgcgaaga ttttccgtgc
    11881 tggctgcatc atccgtgcgc agttcctgca gaaaatcacc gatgcttatg ccgaagatcc
    11941 gcagatcgct aacctgctgc tggctccgta cttcaagcaa attgccgatg actaccagca
    12001 ggcgctgcgt gatgtcgttg cttatgcagt acaaaacggt atcccggttc cgaccttcgc
    12061 cgctgcggtt gcctattacg atagctaccg tgccgctgtt ctgcc
//

</pre>