<pre>LOCUS       KJ778806               10685 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain H 510a serotype O101:H33 O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ778806
VERSION     KJ778806.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10685)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10685)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Swimley,M.,
            Baranzoni,G.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="H 510a"
                     /serotype="O101:H33"
                     /db_xref="taxon:562"
     misc_feature    1..10685
                     /note="O-antigen gene cluster"
     gene            184..1200
                     /gene="galE"
     CDS             184..1200
                     /gene="galE"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="AIG62824.1"
                     /translation="MTILVTGGAGYIGSHTVLQLIEGGYDVVVLDNLSNSSQESLHRV
                     GSLVSKNIPFIKADINDKQKLEEVFKNYDIDAVIHFAGLKSVGESVKKPLEYYINNVS
                     GTLTLLEVMKQANVYKFIFSSSATVYGNHSPVPNLEEYPIGNASCPYGTTKVMLEQVL
                     SDYAKSDDKLKIIALRYFNPTGAHPSGLIGEDPNGIPNNLVPYISQVAIGKLEKLSVY
                     GGDYETIDGTGVRDFIHVVDLAAGHVSALRYIDKLQGYDIFNLGTGTGVSVLQVIDAF
                     EKASNQKVNYEIVARREGDIGSSWADVSKAERILGWKSKYNIEDMMVHTWNWQKQNPN
                     GYGS"
     CDS             1234..3450
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferases group 1"
                     /protein_id="AIG62823.1"
                     /translation="MNLFFIVDKLDWAFGRIAQNIATELSSFNVKIVCTDDFKTVADF
                     AMHMDKNVKEKSILHFFWRDYLMEVMNFTQRNPQYKEILIRNIITTHIPDHLFTYDDI
                     ANYERRIPLMNFVDGYFVTSGKLKELYSELSDFPPPHSVIYDNPEIPMSEVKSYDNHD
                     RKKIVWIGNSKWGEYLGYQDYKGLNTIVLPALDKLKKSGYTFDYIEFDSSKGKTDREV
                     ILKTLSDADILLISSKCEGTPLPLLEAMANSCAVVSTDVGIANEVLPEKQLQYIVSRD
                     AEAFYYSLKGLLDDQRGLEELQRMNYIAYRQQFVDDSLIGDKWALFFKDMCSKSQNEF
                     KNIFLREQKIPSFKERLISKAIYNLSRLALRLNLMNTLKQYSLLRKIYYKVAGTTTNQ
                     VDYDLLDEFYSNAISDKEVLAFYSAYWSGVATSTASFFKDDSIQYPYYSYEFPQVSNH
                     VYLNRLAEKLAESKTLKSVIMSGGTQLQMELAKKIKALSPKIKIFFAWHGSPAQWVDS
                     SQYSTFDGWYELYRERIADGVISFKPELDEILNAYGIRAFSVSNYAVSGSSFSKKLAA
                     PDSANFRVGLFAAMFSWYKNPFPQLLAIGSLKGCELVTNLHLEHNMKWVTSNIKLTTL
                     SENLNNKRFIELLSRLHVICYVTNTECSPMIALESASVGTPCIVGPAGNIYKGFPELE
                     LYLVEKEVDNPTAIRNRLQLVRDNYEEITVLLNAFVLEYNSRLELIKEKMYKELKQ"
     gene            3447..4226
                     /gene="wzm"
     CDS             3447..4226
                     /gene="wzm"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ABC transporter permease"
                     /protein_id="AIG62822.1"
                     /translation="MKESLTDIYLSIKSWHLWTTLGWLELRQRYKRSIVGPFWITLSM
                     AVLIIALGVIYGELFKLEVKEYLPMLAIGLVFWSFISGVINEGCVTFISSSSFISQLP
                     TKKFIYIMQMLWRNFMMLCHNIIIVIGVLIIFNVFSLRNFPLFILGFMIVAVNLIWIA
                     TLFAMISVRFRDFPQMVMSFMQVVFYITPILFSGTMLAKYSLLLKLNPFAWFIEIIRS
                     PLVGKMPELYCYYNSIIFACVGTIISLITFAKCRNRIAYWV"
     gene            4233..4985
                     /gene="wzt"
     CDS             4233..4985
                     /gene="wzt"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen export system, ATP-binding protein"
                     /protein_id="AIG62821.1"
                     /translation="MSHLIDLKDVGVEFPIYDAKSRSFKSQVANLTRGVISSKKESST
                     VTVKSLDGLTFSLKEGDKLGLIGNNGAGKSTLLKLLAGIYEPTSGTIKRKGKIVPLLD
                     IALGMDDDSTGYQNIKLRGLLLGMTNAEIESKIEEIACFTELGDYLNLPIRVYSTGMR
                     VRLAFAVSTSVDPDVLLLDEIIGTGDASFLNKAKKRFEELQERAKVVVLSSHDNNVIL
                     KTCNKVLWLEGGKPKMIGDPEEVLNKYMDSVI"
     gene            5002..6081
                     /gene="pglJ"
     CDS             5002..6081
                     /gene="pglJ"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylgalactosamine-N,
                     N'-diacetylbacillosaminyl-diphospho-undecaprenol
                     4-alpha-N-acetylgalactosaminyltransferase"
                     /protein_id="AIG62820.1"
                     /translation="MKIDYVITGMNVGGAEMQVVELLLELKRCGHSVRLISLTPPVDL
                     IDRLIRNNIPVVSLGMKSKLGLPFAAFKLARLMRQNSPDIVHSHMFHANILVRLVRIF
                     LVDSTSIICTAHSIREGGSARDWLYRLTNKLSDLNTVVSDAARLRFINDKVFPSEKTL
                     TVYNCINIDHFRIIDRQSRKRFRWVTVGRLVKVKNQLLILQAMMKLPLSELIIIGEGE
                     ERLSLQTFIDQNSLSERVTLLGKKDNVVDYYHDSDAFVLASDYEGFALVVAEAMACGL
                     PVVATNCGGPAEIIGNGQDFGITISVNDVHHLTAAMQQIESLDVEQRRGSGYRARERV
                     IEKFSTKKIVSQWEDIYLRIQKNRI"
     gene            6091..7245
                     /gene="pglA"
     CDS             6091..7245
                     /gene="pglA"
                     /codon_start=1
                     /transl_table=11
                     /product="N,
                     N'-diacetylbacillosaminyl-diphospho-undecaprenol
                     alpha-1,3-N-acetylgalactosaminyltransferase"
                     /protein_id="AIG62819.1"
                     /translation="MKIALIGTTAESLIQFRKELINLLHKNGHVVYAFAIDYDDVTKE
                     KVKSFGAIPVHYCFSRTGLNPFSDIINTYKLYKILKSLKLDLVFSYFSKPAIFGTLAA
                     KFARIQKRYAMLEGLGFFFTNSGGQVSLRNKLLKKILVSLYKLSFRHIESLILLNADD
                     KRDLLVNAKIQVKKVHILGGIGLNMSDYPYCPPPVDPVSFIFVARFLQEKGVYEFIEA
                     AQVVKKKFPETHFCMLGHLDLHNPGSLTTERLNDLKCNNIIELPGHVDNVQEWLAKAS
                     VFVLPSWREGFPRSTQEAMAMGRAVITSDVPGCRDTVVDGVNGFLIKPRSSHALAEKM
                     LCFLHQPELITQMGNASHQIALQQFDSYIVNSKLMKILRVDDENTKSNDC"
     CDS             7294..8442
                     /codon_start=1
                     /transl_table=11
                     /product="putative S-adenosylmethionine-dependent
                     methyltransferase/MSMEI_2290"
                     /protein_id="AIG62818.1"
                     /translation="MERLNLSEKPAHSLQEAAIHCARYANILHLVKDKVVLDIACGEG
                     YGSALLMKAGAKRVVGVDISQESIEHAKKLFGGYNVEFIVSDACTISERYGEDFFDMV
                     VSIETIEHINTPEVFLSAIKKTAKENAIFYITCPNDYWYYPNDEQSNPYHVRKYIFQE
                     FTELSTVILGKNVQWAYGCSAFGFCTVSANNRGFEKIGSSWMEISDSENSITVLNTDI
                     DAVSENNCSFFVGLWNAPETNFSNSTFPISMNAYSRMTEEFESNVSLVLPEEQAENKK
                     AISILKQDMKKLTMDLRKSNLLLFALKIENDALRTNISELQQQLARKIAMYDEVTNTN
                     NNLVTTNSSLMERVSQMEIPYYRYVRLSALLPDFLKKMILKIMRLIRK"
     gene            8445..10526
                     /gene="tuaC"
     CDS             8445..10526
                     /gene="tuaC"
                     /codon_start=1
                     /transl_table=11
                     /product="teichuronic acid biosynthesis
                     glycosyltransferase"
                     /protein_id="AIG62817.1"
                     /translation="MSARLKLLPGVKSTEYVYSDLHALLDSNAESSFGPNIGSDGSNL
                     TITCLSMSRVFLTEKLVNSIYQHIPNFKGDILIVDNGSTLEELSVLQTLSDRIPLHIR
                     VIELGNNFGVSGGRNKALEHIKTEWAMFLDNDIYFINNPFPRLQKDISKLGCHFINMP
                     LLDSDGETLFANGGNIFLHIDNDSVHVGAGSACVQEKITTYDGEGFLSTFLFGGASVI
                     KIDSLKSLGKYDENMFIGFEDIDLSLRLYQSGMKVGTCGAISLIHDHPKPSSNKDKDY
                     EKIRFTKSILKESADYLENKWGMVFWSAPVDDWLEEKQRSFELITQKESNYSQIDDNK
                     QNVICDFPVAKPRIALIIDTENWAFSNIANQIVNHLSDSYDFTIIPTEIIDNISQVIM
                     MTKNHDITHFFWRESLRLIYDECYINYNRAIGFDELSFKKQYLDGRIITSSVYDHLFL
                     DEQAIKLRKTFYNDLITAYTVSSSKLYDIYSSIAEYPNPSVLAEDGVDLKLFYPLDLE
                     RFDNIESRTLRVGWAGNSKWAGEVEDFKGYHSLLKPAVEQLQSEGLNIELVLADRQLG
                     FIPHDEMVNYYSQIDVYVCPSKIEGTPNPVLESMACGVPVISTDVGVVNDAFGDMQKE
                     WILPVRSKDVLIDKLRDFYNRRNTVVKCLSSENLQQIKKWDWKVKSENFRIFFDKILE
                     SKKHSKINTEM"
ORIGIN      
        1 aaaaattgga gtgtatagta tgctggtagc tgttgagcca ggggcggtag cgtgtctgaa
       61 taaattaaca tttcggtgtt aaaataaggt aacgttatgt taaaggctaa cttacaacaa
      121 atataagacc aaataattga atacgtaatt agcataaaag attaatacaa tttcggagac
      181 gtcatgacta tacttgtaac aggcggcgct ggttatattg gttcacatac cgttctacaa
      241 ttaattgagg gcgggtatga tgttgttgtg cttgataacc tgagtaattc atcccaagag
      301 tcactacatc gcgttgggtc tttggtttcg aaaaatattc cattcattaa ggctgatatc
      361 aacgataaac aaaagcttga agaagttttt aaaaactacg atattgatgc agtgattcat
      421 tttgcaggtt taaaatctgt tggtgaatca gtaaagaaac cactggaata ttacattaat
      481 aatgtcagtg gaacccttac cctgcttgaa gttatgaagc aagcaaatgt ttacaaattc
      541 atttttagtt cgtctgcgac tgtttatggt aatcattctc cggttcccaa tctggaggaa
      601 tatcctattg gaaatgcttc atgcccctat ggaactacta aagtcatgct ggagcaggtt
      661 ttatcagact atgcgaagtc agatgataaa ttaaaaatta ttgctctgcg ttattttaat
      721 cctacaggtg cgcatcctag tggtcttatt ggggaggacc cgaacggcat tccaaacaat
      781 cttgttcctt atatttctca ggttgctatt ggcaaattag aaaaactgtc cgtttatggt
      841 ggcgattatg aaactattga tggaacagga gtacgcgact ttatccatgt ggtagatctt
      901 gccgcaggcc atgttagtgc tttaaggtat attgataaat tacaaggata tgacattttt
      961 aatcttggta ctggcactgg tgtatcagtt ttacaagtaa tcgatgcctt tgaaaaagca
     1021 agtaatcaga aagttaatta tgaaattgtc gccagacgtg agggcgatat aggatcttct
     1081 tgggctgatg tatctaaagc agaaaggatt ttaggctgga aatccaagta caatattgaa
     1141 gatatgatgg tccatacctg gaattggcaa aaacaaaacc ctaatggcta tggaagttag
     1201 tacctattga tttaatatta taatggcgta tcaatgaatt tatttttcat agtcgataag
     1261 ttggattggg cattcggtag aatagctcaa aatatagcaa cagaattaag tagctttaat
     1321 gtaaaaatag tttgcaccga tgattttaaa actgtggcag actttgctat gcacatggat
     1381 aaaaacgtaa aagaaaagag tatattacac ttcttttggc gtgattatct tatggaagtg
     1441 atgaacttca ctcagcggaa tccccaatat aaagaaattt tgatcagaaa tattatcaca
     1501 acgcatattc ctgatcatct ttttacttat gatgatatcg caaactatga gagaagaatt
     1561 cctcttatga attttgtgga tgggtatttt gttacatccg gaaaattgaa agagctatat
     1621 tctgaactgt ctgattttcc gcctcctcat agcgttatct atgataatcc agaaattcct
     1681 atgagcgagg taaaaagcta tgataatcat gatagaaaaa aaatcgtatg gattggcaat
     1741 agtaaatggg gagaatacct cggttaccaa gattataaag gcctgaatac aatagttttg
     1801 ccggctctag ataaattaaa aaaatctgga tatacgtttg attatattga gtttgatagt
     1861 tctaagggca agaccgatcg tgaggtaatt cttaaaacac tttctgatgc agatattcta
     1921 ctgatcagtt cgaaatgtga aggtacacca ttgccacttc ttgaagcaat ggcaaacagt
     1981 tgtgctgttg tgtcgacaga tgttggtata gcaaatgaag tattacctga aaagcagtta
     2041 cagtatattg tgtctcggga tgcagaggca ttttattatt cattgaaagg tcttcttgac
     2101 gatcaacgcg gtcttgaaga gttacaaaga atgaattata tagcgtatcg tcagcaattt
     2161 gtagatgata gccttatagg tgacaaatgg gcattgttct tcaaagatat gtgttcaaaa
     2221 tcgcaaaatg aatttaagaa tatattcctt cgcgagcaga aaattccatc atttaaagaa
     2281 cgattgatat caaaagctat ttataatcta agcagactag ctcttcgttt gaatttgatg
     2341 aatacgctaa aacaatatag cctgctacga aaaatatatt ataaggttgc aggaacaact
     2401 acaaatcaag ttgattatga tttgttggat gaattttata gtaatgcaat atcagataaa
     2461 gaagtattag cattttactc tgcatattgg tctggtgttg ccacatcaac agctagtttc
     2521 tttaaagatg atagtatcca atatccatat tattcatatg agttcccaca ggtttctaat
     2581 catgtctatc taaacagatt agcagaaaaa cttgctgaat ctaaaacact aaaaagtgtg
     2641 atcatgagcg gtggaacaca attacaaatg gaacttgcca aaaaaattaa ggcattaagt
     2701 ccgaaaataa aaatattttt tgcatggcat ggttcacctg ctcagtgggt tgactcctcg
     2761 caatattcaa cgtttgatgg atggtatgaa ctttaccgag agcgaattgc tgatggggtc
     2821 atctcgttta agcctgagct agatgaaata ctcaatgctt acggtattcg tgcattctca
     2881 gtatcgaatt atgctgtatc aggctcctcg ttttctaaaa aattagctgc acctgattct
     2941 gccaatttcc gcgtaggttt gttcgctgca atgttcagtt ggtataaaaa tccatttcct
     3001 caattactag caattggttc attaaaagga tgtgagctag ttacaaactt gcatcttgaa
     3061 cataatatga agtgggttac ttcaaatatc aagcttacga cattatcaga aaatttaaat
     3121 aataagcggt ttatcgaact gctatctcgt ttacatgtta tatgctatgt aacgaatacg
     3181 gaatgttcgc caatgatagc gttggaatct gcaagtgtcg gaactccatg cattgtgggg
     3241 ccagctggaa acatatataa aggttttcct gagttagaat tgtatttggt tgaaaaagag
     3301 gttgataatc caactgcaat caggaatcgg ttacaattag ttcgtgataa ttacgaggaa
     3361 attacagttt tgcttaatgc atttgttttg gaatacaatt cccggctaga attaattaaa
     3421 gaaaagatgt ataaagagtt gaagcaatga aagaatcact aacagatata tatttgtcaa
     3481 taaaatcgtg gcatctttgg accacattag gttggttaga gttaagacag agatataaaa
     3541 gatccattgt tggaccattt tggattacac taagtatggc ggtccttatt attgccttag
     3601 gggtgattta tggagaacta tttaaactag aagttaaaga atatcttccg atgcttgcaa
     3661 ttggtttggt tttctggagc tttattagtg gcgttataaa tgaaggatgt gttactttca
     3721 tatcgtccag ctcctttata agtcaattgc caactaaaaa attcatatat attatgcaaa
     3781 tgctatggcg aaattttatg atgctttgcc ataatataat aatagtgatt ggtgtgttga
     3841 ttatattcaa cgttttttcg ttgaggaatt tccctctttt tatacttggc tttatgatcg
     3901 tagctgttaa cttgatctgg attgcaactt tgttcgcgat gatttctgta cgttttagag
     3961 attttccgca aatggtcatg agctttatgc aggttgtctt ttacataact ccaatattat
     4021 tttctggaac aatgctggct aaatatagtt tactgctgaa gttaaatcca tttgcttggt
     4081 ttattgagat tattcgatcg cctttagtgg gcaaaatgcc agagctatac tgctattata
     4141 attccattat ctttgcatgc gtaggaacaa ttatctctct aattacattt gcaaaatgta
     4201 gaaaccgcat tgcatattgg gtgtaaatta ttatgtctca tttgattgac ttgaaagatg
     4261 tgggtgttga atttcccatt tacgacgcta aaagtagatc gtttaaatcg caggtggcaa
     4321 atttaacccg tggtgtaatt tcttcaaaaa aagaaagcag cacagttacg gttaaatcat
     4381 tggacggttt gacgtttagt ctgaaagaag gtgataaact cggacttatt ggaaataatg
     4441 gtgcgggaaa atctacgcta ctgaaactct tggctggtat ttatgaacca acatccggca
     4501 caattaaacg aaagggtaag atagttcctc tgttggacat cgctctaggg atggatgatg
     4561 attccacagg ataccaaaat ataaagctac gtggtttatt actaggtatg acgaatgcag
     4621 aaatcgagtc caaaatagaa gagatcgctt gttttactga actaggtgat tatctgaatc
     4681 taccaattcg tgtctactct accggcatgc gcgtgagatt agcattcgca gtatcaactt
     4741 cagttgatcc agatgtactt ctactcgacg aaattattgg tactggcgat gcttccttct
     4801 taaataaagc aaagaaaaga ttcgaggaat tgcaggagag ggcaaaggta gttgtgttgt
     4861 cttcgcatga caataatgtc attctgaaaa cttgtaataa agtcctgtgg ttagagggcg
     4921 gtaaaccaaa gatgattgga gatccggaag aagtactaaa taaatatatg gatagtgtga
     4981 tttaaaaaac aggtggtatg agtgaaaatt gattatgtca ttacaggcat gaatgtcgga
     5041 ggcgcggaaa tgcaggtagt agaactgctt ctcgagctta aacggtgtgg gcattctgtt
     5101 cggttaattt cgcttacacc accagttgat ttgatcgata ggctaattag aaataacatc
     5161 cctgttgttt cattggggat gaagtccaaa ttaggtttac catttgctgc atttaaatta
     5221 gccaggttga tgagacagaa ttctccagat attgtgcatt cgcatatgtt tcatgccaat
     5281 atcctagtac gtcttgtaag aatatttttg gtggatagta catctattat ttgtactgcg
     5341 cacagcattc gtgaaggtgg cagtgctaga gactggcttt atcgtttgac taataaatta
     5401 agtgacttga atacagtcgt aagcgatgcg gcacggctgc ggtttattaa cgacaaagta
     5461 tttccatcag agaaaacact tactgtttat aattgtatca atattgatca ttttcgtatc
     5521 attgatagac aaagcaggaa acgatttcgc tgggtaaccg ttgggcggct tgtaaaagta
     5581 aaaaatcaac ttttgatttt gcaagcgatg atgaagttgc cacttagtga actcatcata
     5641 attggagaag gcgaggaacg actctcatta caaacattta tcgatcaaaa ttctctaagc
     5701 gagcgagtta ctctcttggg taaaaaagat aatgttgttg attattatca tgattctgat
     5761 gcttttgtgc tggcctcaga ctatgaagga tttgcactcg tcgtggccga agctatggca
     5821 tgcggattgc cagttgtcgc aacaaactgt ggtgggcctg ctgaaattat tggaaatgga
     5881 caggattttg gcataacaat ttcggttaat gatgtacatc acttaaccgc tgcgatgcaa
     5941 caaattgaat cattagatgt tgaacagcgt agaggaagtg gctatcgtgc tcgggaaaga
     6001 gtcattgaga aattttctac taaaaaaatc gtatctcaat gggaagatat ctacctgcgc
     6061 atacagaaaa acagaattta gaattcacct atgaaaatag cattaatcgg tactacagca
     6121 gaaagtctaa tccaattcag aaaagaactg attaatttat tacataaaaa tggccatgtt
     6181 gtgtatgcgt ttgcaattga ttatgatgat gtaacaaaag aaaaagttaa atcatttggt
     6241 gctataccag tacattactg tttcagtcga acagggctaa atccattttc tgatattatc
     6301 aatacctata aattatataa gattctaaaa tcacttaagt tggacttggt attttcatat
     6361 ttttcaaagc cagccatttt tggtacttta gcagcaaaat ttgcaagaat acagaagcgt
     6421 tatgcgatgc ttgaagggct tggttttttc tttaccaatt ctggagggca ggtttcatta
     6481 cggaataaat tactaaagaa aatattagta tcactttaca aattaagttt cagacatatt
     6541 gaaagtctta tcttgcttaa tgcggatgat aagcgagacc ttttagttaa tgcaaaaatt
     6601 caagtcaaaa aggttcatat attagggggg attggattaa atatgtctga ttacccctac
     6661 tgcccaccgc ctgttgatcc agtgtctttc atttttgtcg ctagattttt acaagaaaag
     6721 ggagtttatg aatttattga ggctgcacaa gtagtcaaaa agaaatttcc tgaaactcat
     6781 ttttgcatgc tgggtcatct agatctccat aatccgggga gcttaactac agagcggctt
     6841 aatgatttga agtgtaataa catcattgag ttacctggcc acgttgataa cgtgcaggag
     6901 tggcttgcta aagcgagtgt atttgtattg ccttcctggc gtgaagggtt tccgaggagt
     6961 acacaggagg cgatggcaat gggacgtgct gtcataacca gcgatgtgcc aggctgccga
     7021 gatacagtag ttgatggtgt taatggattt ttgattaagc cacgttcttc tcatgcgtta
     7081 gcggaaaaaa tgctctgttt tttacatcaa cctgagctaa ttacccaaat ggggaatgca
     7141 agtcaccaaa tagctttaca acaatttgac tcatatatag ttaattcaaa acttatgaaa
     7201 atactgcgcg tagatgatga aaatacaaag tctaatgact gttaaatcat ataaataaaa
     7261 cgttataata aatacaattt agggggggat attatggaaa gattaaattt gagtgaaaaa
     7321 cctgctcact ctttacaaga agcggctatt cattgtgcca gatatgctaa tatcttacat
     7381 cttgttaaag ataaagtagt tctcgacatt gcctgcggcg aaggatatgg ctcagcacta
     7441 ttaatgaaag ctggtgctaa acgagttgtg ggggtggaca tctctcaaga gtcaattgag
     7501 cacgcaaaga aactttttgg gggatataat gttgagttta ttgtaagtga tgcatgtacc
     7561 atctcagagc gttatggtga agattttttt gatatggttg tttcaataga gacaatagaa
     7621 cacatcaata ctcctgaagt ttttcttagt gctatcaaaa aaactgctaa agaaaatgcg
     7681 attttctata taacttgccc taatgattat tggtattatc caaatgatga acaatccaat
     7741 ccgtaccatg tacgaaaata tatttttcaa gagttcaccg aacttagtac agtgattttg
     7801 ggaaagaatg ttcaatgggc atatggttgt tctgcttttg gattttgtac agtctcagca
     7861 aataatcgag gttttgaaaa aataggttct tcatggatgg aaataagcga ttcggaaaat
     7921 tcaattactg ttttaaatac agatattgac gcagttagtg aaaataattg cagttttttt
     7981 gtgggcttat ggaatgcacc tgaaacaaat tttagcaata gtacattccc aataagtatg
     8041 aatgcttatt cgcgcatgac agaagagttc gagtcgaatg taagtctggt acttcctgag
     8101 gaacaggcag aaaataaaaa agctatatca atattaaaac aagatatgaa aaagttaaca
     8161 atggacctga gaaaatcaaa ccttttatta ttcgcattaa aaatcgaaaa tgatgcattg
     8221 cgtactaata tttccgaatt gcaacaacag cttgccagga aaattgcgat gtatgatgaa
     8281 gtaacaaata caaataataa tttagtaact acaaattcat cattgatgga aagagtgtca
     8341 cagatggaaa ttccatatta tcgatacgta aggctatccg cccttcttcc ggattttttg
     8401 aaaaaaatga tacttaaaat tatgcgtttg atcaggaaat aaaaatgtcg gcacgtttaa
     8461 aattattacc aggggtaaaa tcaactgaat atgtgtattc agaccttcat gctttgctcg
     8521 attctaatgc tgaaagctcg tttggtccga acattggtag tgatggttct aacctaacaa
     8581 taacatgttt atcgatgagt agagtttttc ttactgaaaa acttgttaat tctatatatc
     8641 agcatatacc taatttcaaa ggtgatatcc tgattgttga taacggcagt acattagaag
     8701 aactttcagt tttacaaact ttaagtgata ggattccatt acatattagg gttatcgaac
     8761 ttggtaataa ttttggcgta agtggcggaa gaaataaagc gttagagcat ataaaaacag
     8821 aatgggctat gtttcttgat aatgatattt attttataaa caacccgttc ccgagattgc
     8881 aaaaggatat ttcaaaactt ggctgtcatt tcatcaatat gccattgctt gattctgatg
     8941 gagaaacatt attcgcaaat ggagggaata ttttcctcca tattgataat gattcggtgc
     9001 atgttggtgc aggaagtgca tgcgtccagg aaaaaataac tacatatgat ggtgagggct
     9061 tcctttctac atttctcttt ggcggtgcga gtgttattaa aattgactct ctgaaaagtt
     9121 tagggaagta cgatgaaaat atgttcattg gatttgagga tattgattta tcgcttcgtc
     9181 tttaccagtc aggaatgaaa gtaggtacat gtggtgcaat atctttaatc catgatcacc
     9241 ccaaacctag ttctaataaa gataaggatt atgagaaaat tcgcttcacg aaaagtatat
     9301 taaaagagtc tgcggattat cttgaaaaca aatggggaat ggttttctgg tcagctccag
     9361 ttgatgattg gttggaagag aagcaacgta gttttgaact gataactcaa aaagaaagta
     9421 actattcgca aattgatgat aataaacaaa atgtcatttg tgattttcca gtagcaaaac
     9481 cgagaattgc attgattatt gatactgaaa actgggcatt ctcaaatata gcaaatcaaa
     9541 tagtcaatca tctgtcagat tcgtatgatt ttacaataat accaactgaa atcattgata
     9601 atatcagtca ggttattatg atgaccaaaa atcatgatat aactcatttc ttctggcgtg
     9661 aatcgcttag acttatatat gatgaatgct atataaacta taaccgggct attggatttg
     9721 atgagcttag ttttaaaaaa caatacttag atggaaggat aataacaagt agtgtttatg
     9781 atcatctatt tcttgatgaa caagctataa agttaagaaa aacattttat aatgatttaa
     9841 taactgctta tacagttagt tcatcaaaat tatatgatat atactcatca atagccgagt
     9901 atcctaatcc gtcagttctt gctgaagatg gtgtcgattt aaaactattt tatcctctcg
     9961 atcttgaacg ctttgacaat attgagtcga gaacattgcg cgtaggttgg gctggtaatt
    10021 ctaaatgggc gggagaagta gaggatttta aaggttacca ttctttatta aagccagcag
    10081 ttgagcaatt acaatcagaa ggattgaata tagagttagt ccttgctgat cgtcagctcg
    10141 gatttattcc tcatgatgaa atggtaaatt attatagcca aattgatgtc tatgtctgtc
    10201 cttcaaaaat tgagggcaca ccaaaccctg tactcgaatc tatggcatgt ggcgttcccg
    10261 tgataagtac tgatgtcggt gtggttaatg atgcgtttgg tgatatgcaa aaagaatgga
    10321 ttttgccagt tagaagcaag gatgttttga tagataaact tcgggatttt tataataggc
    10381 gtaacacagt ggtaaaatgt ttatcttcag aaaatctgca gcaaataaaa aaatgggatt
    10441 ggaaagttaa atctgagaac ttccgcattt ttttcgacaa aatattagaa tctaaaaagc
    10501 acagcaagat aaacactgaa atgtgatcaa aatatcattt taaccttaac acaggttttt
    10561 tatcattatc gtgagttaat aagatcgtag tatctacacc tgacaggagt atgtaatgtc
    10621 taagcaacag atcggcgtca tcggtatggc agtaatgggg cgcaacttgg cgctcaatat
    10681 tgaaa
//

</pre>