<pre>LOCUS       KJ778805                9918 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain H 509a serotype O100:K-:H2 O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ778805
VERSION     KJ778805.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9918)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9918)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Swimley,M.,
            Baranzoni,G.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="H 509a"
                     /serotype="O100:K-:H2"
                     /db_xref="taxon:562"
     misc_feature    1..9918
                     /note="O-antigen gene cluster"
     gene            115..1197
                     /gene="rmlB"
     CDS             115..1197
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AIG62807.1"
                     /translation="MKILVTGGAGFIGSAVVRHIIKNTQDEVVNVDKLTYAGNLESLT
                     EVSDSARYVFEHADICDKSAMDRIFAEHKPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARAYWSTLDDVAKKAFRFHHISTDEVYGDLPHPDEHPVSTDLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWLRTYGFPTIVTNCSNNYGPYHFPEKLIPLVILNALDG
                     KPLPIYGKGDQIRDWLYVEDHARALYTVVTQGKPGETYNIGGHNEKKNLEVVHTICDL
                     LDEIVPKEGSYRDQIMYVADRPGHDRRYAIDAHKIAAELGWKPAETFESGIRKTVEWY
                     LSNQDWVNNVKSGAYKTWIEQNYGER"
     gene            1200..2099
                     /gene="rmlD"
     CDS             1200..2099
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="AIG62808.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSKEYCGDFSNPE
                     GIAETVRAIKPDVIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKEAAKIGAWVVHY
                     STDYVFPGDGDKPWRETDATAPLNVYGQTKRDGEKALQAYCPNHLIFRTSWVYAGKGN
                     NFAKTMLRLAKERKELSVINDQVGAPTGAELLADCTAHAIRVAMVKPEVAGLYHLVAS
                     DTTTWYDYAALVFDEARKAGIELAIEKLNAVPTSAYPTPARRPQNSRLSTEKFQHNFG
                     LVLPAWDVGVKRMLAELFTTKSL"
     gene            2152..3030
                     /gene="rmlA"
     CDS             2152..3030
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase 1"
                     /protein_id="AIG62809.1"
                     /translation="MATRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIKDILIISTPQDTPRFEQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGN
                     DSCALVLGDNIFYGHDLPKQLEAAVSKGNGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYLEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKKLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            3034..3582
                     /gene="rmlC"
     CDS             3034..3582
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="AIG62810.1"
                     /translation="MNVIKTDIPDVLIFEPKVFGDDRGFFFESFSQKVFEEAVGRKVE
                     FVQDNHSKSCKGVLRGLHYQLEPYAQGKLVRCVAGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSNIAEFVYKTTNYYHPESDRGLIWNDRDIGIVWGDT
                     DNVLLSEKDQKQPSLTELLNQQ"
     gene            3596..3988
                     /gene="tagD"
     CDS             3596..3988
                     /gene="tagD"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="AIG62811.1"
                     /translation="MKRIITFGTFDVFHVGHINIIERAKKLGDYLIVGVSSDELNFSK
                     KQRYPIYNQTDRLKIISSMKCVDEVFIEESLELKLEYIKKYKADILVMGDDWTGRFDW
                     VKTDCEVIYLPRTPSVSTTEIIEIVKSI"
     gene            4001..5242
                     /gene="wzx"
     CDS             4001..5242
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62812.1"
                     /translation="MPKKNVVENIIFLSIIQFANYLAPLMVLPFLSRTLGVEGFGLIM
                     VAFSACTLCFLLTDFGFNLSAPYWIARNSNYRNRVSRTIGAIFLIKFIILLIIGLILF
                     LYVLFLTSNTMHMRLMYTIIYCIAIAQAFQPVWFFQGVEKMKNVTIFMVTAKLSYLIL
                     IFLFVRNKEDDLLVLLCFFISNFIATIIGVGLIWGAGYKIMIPTKKIIADTFKASSSF
                     FLSRLAVGVYTNASTLIVGAASGVVQAGLYSSAEKLYQAGQNLTSPISQALYPYVART
                     GNKNILFKTILLLLIPMILGCSICFIYAAEIIKLIYGASFVNAQHVLRIFLIISIITF
                     VSINLGYPAFAAMGRVNLANYTVMLGGIVQVLLLAILYTINSINAVNVALSVLTTEII
                     VLAVRILLLIKPNINTHEEYR"
     gene            5226..6422
                     /gene="tagF"
     CDS             5226..6422
                     /gene="tagF"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol:poly(glycerophosphate)
                     glycerophosphotransferase"
                     /protein_id="AIG62813.1"
                     /translation="MKNIVKIPVKFVAVCARMLSITLACLIPKEKKLWVFGGWYGQRI
                     SDNPKYLLQYVNKYHAKNIRAVWIYKNANVKAEALKLGIEAYHYKSIKGKLIQLRAGL
                     VLFTHTIHTDLNSKCISWNTKRIQMWHGIPLKKIGFDDNFTASKLKKSKLYRFTLNDI
                     NSYVLSSGNAVSKIFQSAFDESLGKMAETGFPRNDVFFNKQHQKISGKYNVLYMPTLR
                     AGYGATFNLLDKRYDFQFEEIDSFLKEHDITLTLRIHPANNPDAKMVELIAASDSIKM
                     SSSDDVYDEIGMYDCLITDYSSIMFDFALSHKPIIFAAFDIKDYLRDDRGMYFNYEDI
                     ASNNIAFSWQDVMFSLKENINVREHQDYKFLLQFHDDQNLLGKEHYYSRKVYEFLIEK
                     LNIHVS"
     gene            6456..7625
                     /gene="wzy"
     CDS             6456..7625
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG62814.1"
                     /translation="MAFIRFVNFLIIFFISIDMGGELGIRNVSFLLLVLLIAYYIVTC
                     KKIKFRLPYVLLIILFLGYPVASLFIGMSNNFDADTGISQISIFVFAFLYFLKRIIEE
                     DNNLPLMPIQYFNNAMLIISLFYVLMLISYQFFPSIFNSIALYIHDRGAGFAGVRTEN
                     GVGFANIYLKSSLFLIFTYCCFLKRNTIKAAIIILATVITTSKALLLTEVLLLVTKLK
                     GRLISVVVFVSALLLFIAIYFDEIMVVFNYMNETLQGNSETYLVRVHHFEDLVALFSE
                     NPINFLFGFGAGSSFFSSYYGKYIFNIELDHLNAIRKFGLIWFLSFMWLVFFNIIQLV
                     RLKRNELALGLFLSFVLAGTNPVLLSPIFFILLIECHLLTLKVPCSTSGLHNSKV"
     CDS             7634..8575
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="AIG62815.1"
                     /translation="MFNKSKKLAILLSSYNGEQFIEQQLQSLIDMKADFIFDIHIRDD
                     GSSDKTCETIKKFSQKHANIHLYEENNVGVIASFLWLVEKVSGYDYYAFCDQDDFWQP
                     LKALAAIDKFEKHEEITPLVYCSAYDYVDQNLNFIGRFTSKSNFSPSNLLIENCAPGC
                     TMLFNSALREKYLSISAANISNRVVMHDWLFLLLGLHFGMVIYDRNSYLLYRQHANNV
                     IGKKSGLLPIFRSKYKQFIKECKRPQHLLYLQNELFSDITSGQVNSLTHKLSCSFVSA
                     QRNFYSRAKFAFSGKIKRIRKVDDLIFKILFVFGYYK"
     gene            8591..9730
                     /gene="pglA"
     CDS             8591..9730
                     /gene="pglA"
                     /codon_start=1
                     /transl_table=11
                     /product="N,
                     N'-diacetylbacillosaminyl-diphospho-undecaprenol
                     alpha-1,3-N-acetylgalactosaminyltransferase"
                     /protein_id="AIG62816.1"
                     /translation="MSQLKKLHIVLVGNTLWSVYNFRRGLIQSFMSNGHRVTIVGPKD
                     EFTEKLEGLGCDVVDIPISSQGKNPFIDMLLINRLRVVYKRIKPDFVFHYTIKPNIYG
                     SIAAGLSRTKSIAITTGLGYVFNNSSFFTHIISHLYCFAFKFAKEVWFLNEDDRNVFI
                     QRGIISPGKTHILDGEGVDTSFFIPRAKPISHKKSKVTFLLVARMLWDKGVGIYVDAA
                     RKLKEQYPDTEFQLLGACDVDNPSAIPRDIIDNWHEEGVVNYLGMTNDVRTVVSQADC
                     IVLPSYYREGVPRTLMEAASMGKPVLTTDNVGCRNVVIEAQTGFLCKTKDVDSLVDAM
                     NKILHLTPEERSEMGLNGRDYMISRFDERVIIADYYNAIQKYVEV"
ORIGIN      
        1 cactggtagc tgttaagcca gcggcggtag cgtgcaaaat ttctgaagcc gatattaatt
       61 acccggtatc agaacgccaa tatctcgaca aattcaatag acggaacaga tcacgtgaaa
      121 attcttgtga cgggcggggc cggctttatc ggctcagcag tggtacgaca tattattaaa
      181 aatacccagg atgaagtcgt caatgtggat aaactgacgt atgccggtaa ccttgagtcg
      241 cttaccgaag tgagtgatag cgccagatat gtctttgagc atgcggacat ttgcgacaag
      301 agtgccatgg atcgtatctt tgctgagcat aagccggatg ccgttatgca cctggcggct
      361 gagagtcatg tcgatcgttc catcaccggc ccggccgcgt ttattgaaac caatatcgtc
      421 ggtacctatg tattgcttga agcggctcgt gcttactggt ccactttaga tgatgtagcg
      481 aagaaggcgt ttcgtttcca tcatatctcg accgatgaag tgtatggcga tttgccgcac
      541 ccggacgagc atccggtttc taccgatctg ccgctcttta ccgaaaccac agcttatgcg
      601 ccaagcagcc cgtattctgc ttcaaaagct tccagcgatc atctggttcg cgcctggctg
      661 cgtacctatg ggttcccaac catcgtcact aactgctcca ataactacgg cccgtatcat
      721 ttccctgaga aactgatccc gctggtcatc ctgaatgcgc tcgatggcaa accactgcca
      781 atttatggca aaggtgacca gatccgcgac tggttgtatg ttgaagatca tgctcgtgcg
      841 ctctacaccg tcgtaaccca gggtaagccg ggtgaaactt acaacattgg tggtcataac
      901 gagaagaaaa acctcgaggt ggttcatacc atttgcgatc tgctggatga aattgttccg
      961 aaagagggct cttatcgcga ccagatcatg tatgttgccg atcgtccagg ccacgatcgc
     1021 cgctatgcta tcgacgcaca taagattgcc gctgaattgg gttggaagcc tgcagaaaca
     1081 tttgaaagtg gcattcgtaa gacagtggaa tggtatttga gtaaccagga ctgggttaac
     1141 aacgttaaaa gcggtgccta taagacctgg atcgaacaga actacggaga acgataacta
     1201 tgaatattct tctgttcggc aagacagggc aggtgggttg ggaacttcag cgcgcactcg
     1261 ctccattagg taatttaatt gcccttgatg tgcattccaa agagtattgt ggtgatttta
     1321 gcaatccgga agggattgct gagactgtcc gtgctatcaa acctgatgtg atcgttaacg
     1381 cagcagctca cactgctgta gataaagcag aatctgagcc agagtttgcg cagttactga
     1441 acgcaaccag cgttgaagct attgcgaagg aagctgcaaa aattggggcc tgggttgttc
     1501 attattcaac agattacgtt tttccgggcg atggcgataa accatggcgt gaaacggatg
     1561 caacagcgcc tttgaatgtt tatgggcaga ctaaacgtga tggtgaaaaa gcgctgcaag
     1621 catattgccc taaccatctt attttccgta ccagctgggt ctacgcgggt aaaggtaaca
     1681 atttcgcgaa aaccatgctg cgtctggcga aagagcgtaa agaactttct gttatcaacg
     1741 atcaggttgg tgcacctaca ggtgcagaat tactggcaga ctgtaccgcg catgctattc
     1801 gtgtagcgat ggtaaaacca gaagtggctg gtctttatca cttggtagca tccgatacga
     1861 caacctggta tgactatgca gcactggtat ttgatgaagc cagaaaggca ggcatcgaac
     1921 tggcgattga aaagctcaac gctgtgccga caagcgctta cccgacacct gctcgtcggc
     1981 cgcagaattc gcggttaagt acagagaaat tccagcataa ctttggtctg gtattgcccg
     2041 catgggatgt tggcgtgaag cgcatgctcg ctgagctttt taccactaaa tcactttaac
     2101 cattagataa atgcccggat tcgggcattt tgcgtttaca gagaaattat aatggctacg
     2161 cgtaaaggta ttattcttgc tggtggatcc ggcactcgtc tctaccctgt gaccatggcg
     2221 gtgagtaaac aactgctgcc catttacgac aaaccaatga tttactatcc gctgtctacg
     2281 ctgatgctgg cagggattaa agacattctg atcatcagta cgccacagga taccccgcgc
     2341 tttgaacagc ttctgggcga cggcagtcag tgggggttaa accttcagta taaagtacag
     2401 ccaagccctg atggactggc tcaggccttt atcattggcg aagagttcat tggtaacgac
     2461 agctgtgcgt tagtgctggg cgataatatt ttctacggcc atgacctgcc taaacagctg
     2521 gaagccgcag tgagtaaagg caacggtgca actgttttcg cctatcacgt caacgatcct
     2581 gaacgttatg gtgtggtcga gttcgacaaa aatggtacgg ccatcagtct ggaagagaag
     2641 ccgctggaac ctaaaagcaa ctatgctgta acgggtctct atttctacga caatgatgta
     2701 gtggaaatgg cgaagaacct taaaccttca gcgcgcggtg agttggaaat taccgatatt
     2761 aaccgtatct acctggagca ggggcgtctg tccgttgcga tgatggggcg tggctatgca
     2821 tggctggata cgggtactca tcagagcctt attgaggcaa gtaattttat tgcgacgatt
     2881 gaagagcgtc aggggcttaa ggtttcttgc ccggaagaga ttgcgtatcg caaagggttt
     2941 attgatgcgg agcaggtgaa gaagctggct gagccgctga agaaaaatgc ctatggtcaa
     3001 tacctgctta agatgattaa aggttactaa gacatgaatg ttattaaaac agatatccca
     3061 gatgtactta tttttgagcc aaaagttttt ggtgacgatc gtggtttttt ctttgagagt
     3121 ttcagccaga aagtattcga agaggcagta gggcgaaaag ttgagtttgt acaggataat
     3181 cattctaaat cctgcaaggg tgtgcttcgc ggtttacatt atcaactgga accgtatgcg
     3241 cagggaaaac tggtgcgctg tgttgctggt gaagtatttg atgttgcggt cgatattcgt
     3301 aaatcctctc cgacttttgg aaaatgggtt ggcgtgaatc tttccgctga gaataaacgt
     3361 cagttgtgga tcccagaagg gtttgctcat gggtttcttg tgctgagtaa tattgctgaa
     3421 tttgtatata aaactacaaa ttactaccac cctgaaagcg atagggggct tatctggaat
     3481 gatcgtgata taggtattgt atggggtgat acagataatg tcctactttc agaaaaagac
     3541 caaaaacagc cttctcttac tgaactttta aaccaacaat aggatgtgct gcactatgaa
     3601 acgaattatt acttttggca catttgatgt atttcatgta ggccacatca atattattga
     3661 gagagcgaaa aagctaggtg attacttaat tgtaggggtc tcttcagacg agcttaattt
     3721 tagcaaaaaa cagcgatatc caatatataa ccaaactgat cgtttaaaaa taattagttc
     3781 aatgaaatgt gttgatgagg ttttcattga ggaatctttg gagttgaaat tagagtacat
     3841 aaaaaaatac aaagctgaca ttttggttat gggagatgat tggactggaa ggtttgattg
     3901 ggtaaaaaca gattgcgaag tgatttattt accaagaaca ccttcggttt caaccacaga
     3961 aattatagaa atcgttaaat ccatttagtg gaagacaatt gtgccaaaaa aaaatgttgt
     4021 agaaaatatt atttttttat caataattca attcgccaat tatcttgcac ctttgatggt
     4081 cttacctttc ctcagtcgca ctttgggcgt ggaggggttt gggctcatta tggtagcatt
     4141 ttctgcatgt acattatgtt ttctcctcac tgattttggc tttaatttat ctgctccata
     4201 ttggatagct aggaattcaa attataggaa tagagtgtcg cgaacaatag gcgctatatt
     4261 tttaattaaa tttataatat tactaattat aggattaata cttttcttgt atgtattatt
     4321 cttaacgagt aacacgatgc atatgagatt aatgtataca ataatttatt gtatagctat
     4381 tgcacaagca ttccaaccag tatggttttt tcagggggtt gagaaaatga aaaatgtgac
     4441 catatttatg gtcactgcaa agttatctta tttaatactt atatttctct ttgtgagaaa
     4501 taaagaagat gaccttctgg ttctgttatg cttctttata agtaatttta ttgcaacgat
     4561 tattggtgtc gggcttatct ggggggcagg ttataaaatc atgattccga caaaaaaaat
     4621 cattgccgat actttcaaag caagttcctc cttcttcctt tcaagattgg cagtcggtgt
     4681 ttatacaaat gctagcacac tgattgttgg tgcagcttcg ggtgtggttc aagcgggttt
     4741 gtattcaagt gcggaaaaat tatatcaagc aggtcaaaat ttaacatctc caataagcca
     4801 agcattgtat ccatatgttg caagaacagg aaataaaaat attcttttca aaacgatctt
     4861 actacttttg attccaatga tattgggctg ctcaatctgt tttatttacg cagccgaaat
     4921 aatcaaatta atatatggtg ccagttttgt taatgcacaa catgtattaa gaatattttt
     4981 gataatatca ataattactt ttgttagtat aaatttgggt tatcctgcct ttgcggcaat
     5041 ggggagagtt aatctggcta attatacagt aatgcttggt gggatcgtgc aagttttact
     5101 gttagcgata ctttatacta taaatagtat taatgctgtg aatgttgctt tgtctgtact
     5161 tacgacagaa attatagttt tagccgtaag aatattatta cttataaaac caaatattaa
     5221 tactcatgaa gaatatcgtt aaaataccgg ttaaatttgt ggctgtatgc gctcggatgc
     5281 tatcaataac tttggcatgt cttataccaa aagaaaaaaa actctgggtt tttggtggat
     5341 ggtatggaca acgaatttct gacaacccaa aatacttact ccaatacgta aataaatatc
     5401 acgcgaaaaa tattcgagct gtttggattt ataaaaatgc taatgttaaa gcagaggcat
     5461 tgaagcttgg tatcgaagcg tatcattata agagtattaa aggaaaactc atacaacttc
     5521 gtgctggtct ggttttattt acacatacaa tacacacaga tttaaattca aagtgtatct
     5581 cctggaatac aaaacgtata caaatgtggc acggtatacc tcttaaaaaa ataggatttg
     5641 atgataactt cacagcttca aaacttaaga aaagtaagct gtatagattt acactcaacg
     5701 acattaattc ttatgtgtta agttcaggaa acgccgtatc gaaaatcttt caatctgctt
     5761 ttgatgaatc acttggaaaa atggctgaaa ctggctttcc gagaaatgat gtttttttca
     5821 ataagcaaca ccagaaaata agtggtaaat ataatgtctt gtatatgcca accttaaggg
     5881 ctggttatgg agcaacgttt aatttattgg ataaaagata tgatttccag ttcgaagaaa
     5941 tagattcttt tttaaaggag catgatataa cactgacatt gagaattcac cctgcaaata
     6001 atcctgatgc taaaatggtt gaactgatag cagcaagtga cagtataaaa atgtcatcgt
     6061 cggatgatgt ttatgatgaa attgggatgt atgattgttt gattaccgat tattcaagta
     6121 taatgtttga ttttgcactg agtcataaac caattatctt tgctgctttt gatattaaag
     6181 attatctcag agacgacaga ggtatgtatt ttaattacga agatattgct agtaataaca
     6241 tcgcctttag ttggcaagat gttatgtttt cattaaaaga aaatataaat gtacgagaac
     6301 atcaagatta taaatttctt ttacaattcc atgacgatca aaatttactc ggtaaagaac
     6361 attattactc acgtaaggtt tatgaattct taattgaaaa gttaaatatt catgtttctt
     6421 agtccaaatg agctacttca cgagtctcta ttaaaatggc attcattaga ttcgttaatt
     6481 ttctgattat ctttttcatt tctatcgata tggggggcga attaggtatt aggaatgttt
     6541 catttctctt gttggtatta ttaattgcct attatatcgt cacttgcaaa aaaatcaaat
     6601 ttaggctgcc ttacgtactc cttattatat tgtttttagg ataccctgtt gcatctttat
     6661 ttattgggat gtctaataat tttgatgcag atactggcat atcacaaatc agtatatttg
     6721 tttttgcatt tttatacttt ctaaaaagga taatcgaaga ggataataat ttacctctga
     6781 tgccgataca atattttaat aatgcgatgc tgattatctc tttgttctat gtcttaatgc
     6841 ttatttcgta tcaatttttc ccttctattt tcaatagtat tgctctttac atacacgata
     6901 gaggtgctgg ttttgctggc gtaagaactg aaaatggagt tggttttgct aatatttatc
     6961 ttaaatctag ccttttttta atatttactt attgctgttt tttgaagaga aataccatta
     7021 aggctgctat tataatattg gctactgtga ttacgacatc taaagcgctt ctattaacag
     7081 aagtattact tcttgtaacc aaattaaagg gtagattgat ttctgttgtt gtttttgttt
     7141 cggctttgtt gttatttatt gcaatttatt tcgatgaaat catggttgta ttcaactaca
     7201 tgaatgaaac attgcaaggc aatagcgaaa catatttggt tagagtgcat cactttgaag
     7261 atctagttgc gcttttctcg gaaaatccaa ttaattttct ttttggtttt ggtgcaggaa
     7321 gttcattttt ctcatcgtat tatgggaagt acatatttaa tatagaatta gaccatctaa
     7381 acgcgattag aaagtttggc ctcatctggt ttttatcttt tatgtggctt gtattcttta
     7441 atattatcca acttgttaga ttaaaaagaa atgaattggc tcttgggctt tttctttctt
     7501 tcgtcctggc aggtaccaac ccagtattac tgagccctat attctttata ttattaatcg
     7561 aatgtcattt attgacactt aaagtaccat gttcaactag tgggctacac aatagtaagg
     7621 tgtaattctt tttatgttta ataaatctaa aaagttagct attttacttt ccagttataa
     7681 tggcgagcaa tttatagaac aacaactaca atctcttatc gacatgaagg ctgatttcat
     7741 tttcgatatt catattagag atgatggttc aagtgataaa acttgtgaaa ctattaagaa
     7801 gttttcccag aaacatgcta acattcattt atacgaagag aataatgttg gagttattgc
     7861 aagttttctt tggcttgtag aaaaagtatc aggctatgat tattatgctt tttgcgatca
     7921 agatgatttt tggcaaccat tgaaagcctt ggcagcaatt gataaatttg aaaaacacga
     7981 ggaaataact ccgctggtgt attgctcagc ttatgattac gtcgatcaaa atcttaactt
     8041 tattggtcgc tttacttcca aaagtaattt ttcccctagt aatctgttaa tagaaaattg
     8101 tgctcctggt tgtacgatgt tattcaattc agcgctgcga gaaaaatatt taagcataag
     8161 tgctgcaaat ataagtaatc gagttgtgat gcatgattgg ctttttttat tattgggcct
     8221 tcatttcgga atggtaattt atgacagaaa ttcctattta ttatataggc aacatgcaaa
     8281 caatgttata ggtaaaaaat ctgggttgct gcctatcttt agatccaagt ataagcaatt
     8341 catcaaagaa tgtaaaagac cgcagcattt actttatctt caaaacgaat tgttctctga
     8401 tatcacctct ggacaagtaa attccttaac gcataaacta agttgtagtt tcgtttccgc
     8461 tcaaagaaat ttttactcta gagctaaatt tgcctttagc gggaaaataa agcgtatccg
     8521 aaaagttgat gatttaatat ttaagatttt atttgttttt ggatattata aataatatgg
     8581 tttggaatca atgagtcaac ttaaaaaatt acatattgtc cttgtaggta atacattatg
     8641 gtcagtttat aatttccgta gaggtctaat tcaaagcttt atgagcaatg gtcatagagt
     8701 gacaatagtt ggacctaagg atgagtttac tgagaaatta gagggattag gctgtgatgt
     8761 tgttgatatt cctatttcat cgcaaggtaa aaatcctttt attgacatgc tacttataaa
     8821 tcgactgaga gttgtttata aaaggattaa accggatttt gtttttcact atactataaa
     8881 gccaaatata tatggctcga ttgctgctgg cttgtctcga acaaaatcaa tagctattac
     8941 taccgggctt ggttatgttt tcaataactc ttcatttttc actcacataa tttcacattt
     9001 gtactgcttt gcatttaaat tcgcaaaaga agtctggttt ttgaatgaag atgatagaaa
     9061 tgtgtttata caaagaggta ttatctcacc tggcaagact catattcttg atggcgaagg
     9121 tgtcgatact agtttcttca ttcctagggc aaaacctatt tcgcataaaa aatcaaaagt
     9181 tacgtttttg ctcgttgcaa ggatgctgtg ggacaaaggt gttgggatat acgttgatgc
     9241 ggctcgtaaa ctaaaagaac agtacccaga tactgaattt cagttgttag gggcctgcga
     9301 tgtcgataat ccgagcgcaa ttccacgtga tattatagat aattggcatg aagagggtgt
     9361 agttaattat ttgggaatga cgaatgatgt gcgcaccgtt gtatcccaag ccgactgtat
     9421 tgtattgcct tcttattatc gcgagggagt tcctcgaaca ttgatggaag cagcatcaat
     9481 gggcaaacca gtacttacga ccgataatgt cggatgtaga aatgttgtta tagaagcgca
     9541 aacaggtttt ttatgcaaga caaaagatgt cgatagttta gttgacgcta tgaataagat
     9601 tctccattta acccctgagg aacgtagcga aatgggtcta aatggtcggg attatatgat
     9661 aagccgcttc gatgaaaggg ttattattgc tgattattat aatgctattc aaaaatatgt
     9721 tgaagtatag ttgcccctaa ggatttgttt atctactgac catccgtatt tgtataaagc
     9781 atcttccgct atccatgatg acttaacctg agttaacatc aggtatacat tcaagccgcg
     9841 cacagttacg gcgaacacac ctgacaggag tatgtaatgt ccaagcaaca gatcggcgtt
     9901 gtcggtatgg cagtgatg
//

</pre>